Restriction map for
pFN21AE4139
(Circular) (Six-base) MAPSORT of: pFN21AE4139.seq Check: 163 from: 1 to: 6370
pFN21AE4139 6370 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3178 3713 1937
Size: 1241 535 4594
Fragments arranged by size:
4594 1241 535
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 3640 4433 5817 6085 1632
Size: 962 1046 793 1384 268 1917
Fragments arranged by size:
1917 1384 1046 962 793 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6173
AflIII A'CryG_T
Cuts at: 1203 5269 6246 1203
Size: 4066 977 1327
Fragments arranged by size:
4066 1327 977
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 2298 1166
Size: 1132 5238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2952 3014 3385 1184
Size: 1768 62 371 4169
Fragments arranged by size:
4169 1768 371 62
AlwNI CAG_nnn'CTG
Cuts at: 2107 2725 4113 5685 2107
Size: 618 1388 1572 2792
Fragments arranged by size:
2792 1572 1388 618
ApaLI G'TGCA_C
Cuts at: 2234 3370 4427 5583 2234
Size: 1136 1057 1156 3021
Fragments arranged by size:
3021 1156 1136 1057
ApoI r'AATT_y
Cuts at: 1493 2381 2429 2715 3679 3905 4057 6166 1493
Size: 888 48 286 964 226 152 2109 1697
Fragments arranged by size:
2109 1697 964 888 286 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2070 3695 1870
Size: 73 112 15 1625 4545
Fragments arranged by size:
4545 1625 112 73 15
BamHI G'GATC_C
Cuts at: 3700 4067 3700
Size: 367 6003
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2257 3691 5146 618
Size: 325 217 627 470 1434 1455 1842
Fragments arranged by size:
1842 1455 1434 627 470 325 217
BanII G_rGCy'C
Cuts at: 1391 2050 3452 3689 1391
Size: 659 1402 237 4072
Fragments arranged by size:
4072 1402 659 237
BbeI G_GCGC'C
Cuts at: 2261 2261
Size: 6370
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3434 3604 3669 928
Size: 971 1535 170 65 3629
Fragments arranged by size:
3629 1535 971 170 65
BbvCI CC'TCA_GC
Cuts at: 2678 2678
Size: 6370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4561 4595 1903
Size: 34 2624 34 3678
Fragments arranged by size:
3678 2624 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3565 4262 5472 3565
Size: 697 1210 4463
Fragments arranged by size:
4463 1210 697
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2266 4981
Size: 107 122 71 631 605 403 191 2715
Cuts at: 4981 136
Size: 1525
Fragments arranged by size:
2715 1525 631 605 403 191 122 107
71
BglII A'GATC_T
Cuts at: 3319 6365 3319
Size: 3046 3324
BlpI GC'TnA_GC
Cuts at: 3787 3787
Size: 6370
Bme1580I G_kGCm'C
Cuts at: 1790 2238 3374 3418 4431 5587 1790
Size: 448 1136 44 1013 1156 2573
Fragments arranged by size:
2573 1156 1136 1013 448 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4101 5059
Size: 416 53 83 399 24 1751 933 958
Cuts at: 5059 442
Size: 1753
Fragments arranged by size:
1753 1751 958 933 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2517 2930 5030 1239
Size: 1278 413 2100 2579
Fragments arranged by size:
2579 2100 1278 413
Bpu10I CC'TnA_GC
Cuts at: 2678 2678
Size: 6370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4495 5360 5658 4495
Size: 865 298 5207
Fragments arranged by size:
5207 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5033 882
Size: 617 3534 2219
Fragments arranged by size:
3534 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4161 493
Size: 711 2957 2702
Fragments arranged by size:
2957 2702 711
BsaBI GATnn'nnATC
Cuts at: 3525 6364 3525
Size: 2839 3531
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2258 4177 4559 5194
Size: 53 83 186 936 725 1919 382 635
Cuts at: 5194 275
Size: 1451
Fragments arranged by size:
1919 1451 936 725 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2592 4803 5201 5475 5622 6033 2592
Size: 2211 398 274 147 411 2929
Fragments arranged by size:
2929 2211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6145
Fragments arranged by size:
6145 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6370
BseYI C'CCAG_C
Cuts at: 2219 2331 5573 6069 2219
Size: 112 3242 496 2520
Fragments arranged by size:
3242 2520 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2912 1163
Size: 573 1176 4621
Fragments arranged by size:
4621 1176 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2039 2150 2266 3820 4581 5609
Size: 275 186 38 111 116 1554 761 1028
Cuts at: 5609 1540
Size: 2301
Fragments arranged by size:
2301 1554 1028 761 275 186 116 111
38
BsiHKAI G_wGCw'C
Cuts at: 2050 2238 2850 3107 3374 4431 4516 5587 2050
Size: 188 612 257 267 1057 85 1071 2833
Fragments arranged by size:
2833 1071 1057 612 267 257 188 85
BsmI GAATG_Cn'
Cuts at: 3881 3974 3881
Size: 93 6277
BsmBI CGTCTCn'nnnn_
Cuts at: 2359 2359
Size: 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2238 2850 3107 3374 3418 3452
Size: 399 260 188 612 257 267 44 34
Cuts at: 3452 3689 4431 4516 5587 1391
Size: 237 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 267 260 257
237 188 85 44 34
BspEI T'CCGG_A
Cuts at: 2592 6033 2592
Size: 3441 2929
BspHI T'CATG_A
Cuts at: 4259 5989 4259
Size: 1730 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3541 3725 844
Size: 2697 184 3489
Fragments arranged by size:
3489 2697 184
BsrBI CCG'CTC
Cuts at: 1394 1823 2454 4257 1394
Size: 429 631 1803 3507
Fragments arranged by size:
3507 1803 631 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3426 3432 4865 5039 65
Size: 1183 2178 6 1433 174 1396
Fragments arranged by size:
2178 1433 1396 1183 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 2163 2254 2265 5014 5201 1700
Size: 110 346 7 91 11 2749 187 2869
Fragments arranged by size:
2869 2749 346 187 110 91 11 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2107 794
Size: 1313 5057
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6370
BstXI CCAn_nnnn'nTGG
Cuts at: 2842 2842
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 3319 3343 3526 3700 4067 4452 4469 5921
Size: 1347 24 183 174 367 385 17 1452
Cuts at: 5921 6007 6019 6365 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1347 385 367 346 183 174
86 24 17 12
BstZ17I GTA'TAC
Cuts at: 3179 3179
Size: 6370
BtgI C'CryG_G
Cuts at: 513 1065 2361 2841 513
Size: 552 1296 480 4042
Fragments arranged by size:
4042 1296 552 480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5631
Fragments arranged by size:
5631 683 56
BtsI GCAGTG_nn'
Cuts at: 3886 4680 4700 3886
Size: 794 20 5556
Fragments arranged by size:
5556 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DraIII CAC_nnn'GTG
Cuts at: 3411 3608 3411
Size: 197 6173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5377 809
Size: 507 4061 1802
Fragments arranged by size:
4061 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2147 3510 3817
Size: 54 1425 50 275 18 317 1363 307
Cuts at: 3817 4706 6335 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1363 889 317 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2147 3817 1537
Size: 275 335 1670 4090
Fragments arranged by size:
4090 1670 335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2315 3024 3390 4300 1353
Size: 962 709 366 910 3423
Fragments arranged by size:
3423 962 910 709 366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4957 5331 5477 387
Size: 1064 138 3368 374 146 1280
Fragments arranged by size:
3368 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2048 2048
Size: 6370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2517 2594 2930 3640 4433 5030 5817
Size: 393 885 77 336 710 793 597 787
Cuts at: 5817 6085 1239
Size: 268 1524
Fragments arranged by size:
1524 885 793 787 710 597 393 336
268 77
EcoNI CCTnn'n_nnAGG
Cuts at: 2681 2681
Size: 6370
EcoO109I rG'GnC_Cy
Cuts at: 2515 2515
Size: 6370
EcoRI G'AATT_C
Cuts at: 2381 2429 3679 4057 2381
Size: 48 1250 378 4694
Fragments arranged by size:
4694 1250 378 48
EcoRV GAT'ATC
Cuts at: 3139 3139
Size: 6370
FspI TGC'GCA
Cuts at: 2393 2552 4876 2393
Size: 159 2324 3887
Fragments arranged by size:
3887 2324 159
HaeII r_GCGC'y
Cuts at: 1678 2208 2261 5517 1678
Size: 530 53 3256 2531
Fragments arranged by size:
3256 2531 530 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3230 3262 3539 3571 3584
Size: 32 135 32 1658 32 277 32 13
Cuts at: 3584 3616 5011 5043 5085 5117 1373
Size: 32 1395 32 42 32 2626
Fragments arranged by size:
2626 1658 1395 277 135 42 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3572 3714 3960 669
Size: 988 281 1634 142 246 3079
Fragments arranged by size:
3079 1634 988 281 246 142
HindIII A'AGCT_T
Cuts at: 748 3652 748
Size: 2904 3466
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KasI G'GCGC_C
Cuts at: 2257 2257
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5484 5668 1305
Size: 4179 184 2007
Fragments arranged by size:
4179 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4548
Fragments arranged by size:
4548 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 3367 4328 4687 4846 518
Size: 648 1132 1069 961 359 159 2042
Fragments arranged by size:
2042 1132 1069 961 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2363 2963 3786 4463 5611 5856 1606
Size: 501 256 600 823 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 600 501 256 245
NaeI GCC'GGC
Cuts at: 2158 2165 2256 2158
Size: 7 91 6272
Fragments arranged by size:
6272 91 7
NarI GG'CG_CC
Cuts at: 2258 2258
Size: 6370
NcoI C'CATG_G
Cuts at: 513 1065 2841 513
Size: 552 1776 4042
Fragments arranged by size:
4042 1776 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NgoMIV G'CCGG_C
Cuts at: 2156 2163 2254 2156
Size: 7 91 6272
Fragments arranged by size:
6272 91 7
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 3067 3584 3728 5273 1132
Size: 97 1838 517 144 1545 2229
Fragments arranged by size:
2229 1838 1545 517 144 97
PasI CC'CwG_GG
Cuts at: 1348 2465 3559 1348
Size: 1117 1094 4159
Fragments arranged by size:
4159 1117 1094
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6370
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PstI C_TGCA'G
Cuts at: 830 2103 2523 2902 3722 830
Size: 1273 420 379 820 3478
Fragments arranged by size:
3478 1273 820 420 379
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2107 2963 2107
Size: 856 5514
SacI G_AGCT'C
Cuts at: 2050 2050
Size: 6370
SacII CC_GC'GG
Cuts at: 2364 2364
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SfcI C'TryA_G
Cuts at: 826 1045 2099 2519 2898 3718 4853 5534 5725
Size: 219 1054 420 379 820 1135 681 191
Cuts at: 5725 826
Size: 1471
Fragments arranged by size:
1471 1135 1054 820 681 420 379 219
191
SfoI GGC'GCC
Cuts at: 2259 2259
Size: 6370
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 2072 3697 1872
Size: 200 1625 4545
Fragments arranged by size:
4545 1625 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4474 5375 5637 820
Size: 197 926 2531 901 262 1553
Fragments arranged by size:
2531 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3584 3728 1132
Size: 2452 144 3774
Fragments arranged by size:
3774 2452 144
SspI AAT'ATT
Cuts at: 5 52 2872 4294 5
Size: 47 2820 1422 2081
Fragments arranged by size:
2820 2081 1422 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6370
StyI C'CwwG_G
Cuts at: 513 1065 2841 2940 3499 3575 3809 513
Size: 552 1776 99 559 76 234 3074
Fragments arranged by size:
3074 1776 559 552 234 99 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4397 4414 4567 4752 5197 325
Size: 1255 2817 17 153 185 445 1498
Fragments arranged by size:
2817 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4616 6328 96
Size: 274 80 33 51 1443 2639 1712 138
Fragments arranged by size:
2639 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6330 6362 6330
Size: 32 6338
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6370
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 2070 3695 1870
Size: 200 1625 4545
Fragments arranged by size:
4545 1625 200
XmnI GAAnn'nnTTC
Cuts at: 4499 4499
Size: 6370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI Bsu36I FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SrfI SwaI XcmI