Restriction map for
pFN21AE4138
(Circular) (Six-base) MAPSORT of: pFN21AE4138.seq Check: 7342 from: 1 to: 6352
pFN21AE4138 6352 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2951 4162 278
Size: 53 83 186 936 1415 1211 2468
Fragments arranged by size:
2468 1415 1211 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3695 1937
Size: 1758 4594
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2464 3544 4415 5799 6067 1632
Size: 832 1080 871 1384 268 1917
Fragments arranged by size:
1917 1384 1080 871 832 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6155
AflIII A'CryG_T
Cuts at: 1203 2865 5251 6228 1203
Size: 1662 2386 977 1327
Fragments arranged by size:
2386 1662 1327 977
AgeI A'CCGG_T
Cuts at: 2982 5183 2982
Size: 2201 4151
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2992 1184
Size: 1808 4544
AlwNI CAG_nnn'CTG
Cuts at: 2644 4095 5667 2644
Size: 1451 1572 3329
Fragments arranged by size:
3329 1572 1451
ApaI G_GGCC'C
Cuts at: 2330 3404 2330
Size: 1074 5278
ApaLI G'TGCA_C
Cuts at: 4409 5565 4409
Size: 1156 5196
ApoI r'AATT_y
Cuts at: 1493 2205 3661 3887 4039 6148 1493
Size: 712 1456 226 152 2109 1697
Fragments arranged by size:
2109 1697 1456 712 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 2840 3092 3677 1870
Size: 73 897 252 585 4545
Fragments arranged by size:
4545 897 585 252 73
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3229 3551 3673 5128 618
Size: 325 217 627 1442 322 122 1455 1842
Fragments arranged by size:
1842 1455 1442 627 325 322 217 122
BanII G_rGCy'C
Cuts at: 1391 2330 2709 2847 3404 3671 1391
Size: 939 379 138 557 267 4072
Fragments arranged by size:
4072 939 557 379 267 138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2951 3149 3279 928
Size: 971 1052 198 130 4001
Fragments arranged by size:
4001 1052 971 198 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2968 3002 4543 4577 1903
Size: 34 1031 34 1541 34 3678
Fragments arranged by size:
3678 1541 1031 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4244 5454 4244
Size: 1210 5142
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BfrBI ATG'CAT
Cuts at: 2621 2621
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2688 3612 4963
Size: 107 122 71 631 605 1016 924 1351
Cuts at: 4963 136
Size: 1525
Fragments arranged by size:
1525 1351 1016 924 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2776 6347 2776
Size: 3571 2781
BlpI GC'TnA_GC
Cuts at: 3769 3769
Size: 6352
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2330 2632 2971 3232 3293 3306 3404
Size: 251 289 302 339 261 61 13 98
Cuts at: 3404 3554 4413 5569 1790
Size: 150 859 1156 2573
Fragments arranged by size:
2573 1156 859 339 302 289 261 251
150 98 61 13
BmgBI CAC'GTC
Cuts at: 1318 2011 2739 2866 1318
Size: 693 728 127 4804
Fragments arranged by size:
4804 728 693 127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 4083 5041
Size: 416 53 83 399 24 849 1817 958
Cuts at: 5041 442
Size: 1753
Fragments arranged by size:
1817 1753 958 849 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 6352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3159 3364 3627 5012 1239
Size: 1920 205 263 1385 2579
Fragments arranged by size:
2579 1920 1385 263 205
Bpu10I CC'TnA_GC
Cuts at: 2112 2573 3321 2112
Size: 461 748 5143
Fragments arranged by size:
5143 748 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3008 4477 5342 5640 3008
Size: 1469 865 298 3720
Fragments arranged by size:
3720 1469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5015 882
Size: 617 3516 2219
Fragments arranged by size:
3516 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3134 4143 493
Size: 711 1930 1009 2702
Fragments arranged by size:
2702 1930 1009 711
BsaBI GATnn'nnATC
Cuts at: 6346 6346
Size: 6352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2948 4159 4541 5176
Size: 53 83 186 936 1415 1211 382 635
Cuts at: 5176 275
Size: 1451
Fragments arranged by size:
1451 1415 1211 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2814 2982 4785 5183 5457 5604 6015 2814
Size: 168 1803 398 274 147 411 3151
Fragments arranged by size:
3151 1803 411 398 274 168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6127
Fragments arranged by size:
6127 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2169 2552 2588 2627 1186
Size: 983 383 36 39 4911
Fragments arranged by size:
4911 983 383 39 36
BseYI C'CCAG_C
Cuts at: 2087 2248 3082 3305 3438 3530 5555 6051 2087
Size: 161 834 223 133 92 2025 496 2388
Fragments arranged by size:
2388 2025 834 496 223 161 133 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 3510 1163
Size: 573 921 853 4005
Fragments arranged by size:
4005 921 853 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2443 3802 4563 5591 1540
Size: 275 186 442 1359 761 1028 2301
Fragments arranged by size:
2301 1359 1028 761 442 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3024 3589 4413 4498 5569 2427
Size: 597 565 824 85 1071 3210
Fragments arranged by size:
3210 1071 824 597 565 85
BsmI GAATG_Cn'
Cuts at: 2313 2621 3863 3956 2313
Size: 308 1242 93 4709
Fragments arranged by size:
4709 1242 308 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2159
Size: 6352
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2330 2427 2632 2709 2847 2971
Size: 399 251 289 97 205 77 138 124
Cuts at: 2971 3024 3232 3293 3306 3404 3554 3589 3671
Size: 53 208 61 13 98 150 35 82
Cuts at: 3671 4413 4498 5569 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 289 251 208 205
150 138 124 98 97 85 82 77
61 53 35 13
BspEI T'CCGG_A
Cuts at: 2814 6015 2814
Size: 3201 3151
BspHI T'CATG_A
Cuts at: 2394 4241 5971 2394
Size: 1847 1730 2775
Fragments arranged by size:
2775 1847 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3081 3707 844
Size: 2237 626 3489
Fragments arranged by size:
3489 2237 626
BsrBI CCG'CTC
Cuts at: 1394 1823 3505 4239 1394
Size: 429 1682 734 3507
Fragments arranged by size:
3507 1682 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4847 5021 65
Size: 1183 3599 174 1396
Fragments arranged by size:
3599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2982 3124 4996 5183 1700
Size: 110 1172 142 1872 187 2869
Fragments arranged by size:
2869 1872 1172 187 142 110
BsrGI T'GTAC_A
Cuts at: 96 2158 96
Size: 2062 4290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2665 794
Size: 1871 4481
BstBI TT'CG_AA
Cuts at: 2122 5156 2122
Size: 3034 3318
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2755 2011
Size: 744 5608
BstYI r'GATC_y
Cuts at: 1972 2776 2832 3682 4049 4434 4451 5903 5989
Size: 804 56 850 367 385 17 1452 86
Cuts at: 5989 6001 6347 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 850 804 385 367 346 86
56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2610 3333 3595 2610
Size: 723 262 5367
Fragments arranged by size:
5367 723 262
BtgI C'CryG_G
Cuts at: 513 1065 2004 2754 2963 3197 3224 513
Size: 552 939 750 209 234 27 3641
Fragments arranged by size:
3641 939 750 552 234 209 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2566 487
Size: 683 56 1340 4273
Fragments arranged by size:
4273 1340 683 56
BtsI GCAGTG_nn'
Cuts at: 2795 3362 3868 4662 4682 2795
Size: 567 506 794 20 4465
Fragments arranged by size:
4465 794 567 506 20
ClaI AT'CG_AT
Cuts at: 1554 2488 1554
Size: 934 5418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6317
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 3799 4688
Size: 54 1425 50 275 18 816 1153 889
Cuts at: 4688 6317 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1153 889 816 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4282 1353
Size: 2929 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3401 4939 5313 5459 387
Size: 1064 138 1812 1538 374 146 1280
Fragments arranged by size:
1812 1538 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2464 3159 3364 3544 3627 4415 5012
Size: 393 832 695 205 180 83 788 597
Cuts at: 5012 5799 6067 1239
Size: 787 268 1524
Fragments arranged by size:
1524 832 788 787 695 597 393 268
205 180 83
EcoNI CCTnn'n_nnAGG
Cuts at: 3001 3152 3320 3001
Size: 151 168 6033
Fragments arranged by size:
6033 168 151
EcoO109I rG'GnC_Cy
Cuts at: 2108 2326 2569 3036 3070 3169 3329 3400 3401
Size: 218 243 467 34 99 160 71 1
Cuts at: 3401 3599 2108
Size: 198 4861
Fragments arranged by size:
4861 467 243 218 198 160 99 71
34 1
EcoRI G'AATT_C
Cuts at: 3661 4039 3661
Size: 378 5974
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 2938 5499 1678
Size: 1260 2561 2531
Fragments arranged by size:
2561 2531 1260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2040 2072 2376 2408 2819
Size: 32 135 32 468 32 304 32 411
Cuts at: 2819 2851 3086 3118 4993 5025 5067 5099 1373
Size: 32 235 32 1875 32 42 32 2626
Fragments arranged by size:
2626 1875 468 411 304 235 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3696 3942 669
Size: 988 281 1758 246 3079
Fragments arranged by size:
3079 1758 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6352
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3567 5466 5650 1305
Size: 2262 1899 184 2007
Fragments arranged by size:
2262 2007 1899 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 10
Size: 54 1425 343 816 3714
Fragments arranged by size:
3714 1425 816 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2060 4310 4669 4828 518
Size: 648 843 51 2250 359 159 2042
Fragments arranged by size:
2250 2042 843 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2506 2641 3199 3469 3768 4445 5593 5838
Size: 900 135 558 270 299 677 1148 245
Cuts at: 5838 1606
Size: 2120
Fragments arranged by size:
2120 1148 900 677 558 299 270 245
135
NaeI GCC'GGC
Cuts at: 3126 3126
Size: 6352
NcoI C'CATG_G
Cuts at: 513 1065 2004 2754 513
Size: 552 939 750 4111
Fragments arranged by size:
4111 939 750 552
NdeI CA'TA_TG
Cuts at: 387 2533 387
Size: 2146 4206
NgoMIV G'CCGG_C
Cuts at: 3124 3124
Size: 6352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NsiI A_TGCA'T
Cuts at: 2623 2623
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2729 3710 5255 1132
Size: 97 1500 981 1545 2229
Fragments arranged by size:
2229 1545 1500 981 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6352
PciI A'CATG_T
Cuts at: 5251 5251
Size: 6352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6352
PfoI T'CCnGG_A
Cuts at: 3267 3341 3267
Size: 74 6278
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PmlI CAC'GTG
Cuts at: 3134 3134
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2569 3070 3329 2569
Size: 501 259 5592
Fragments arranged by size:
5592 501 259
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 3922 3922
Size: 6352
PspOMI G'GGCC_C
Cuts at: 2326 3400 2326
Size: 1074 5278
PstI C_TGCA'G
Cuts at: 830 2567 2670 3357 3704 830
Size: 1737 103 687 347 3478
Fragments arranged by size:
3478 1737 687 347 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
PvuII CAG'CTG
Cuts at: 2641 3469 2641
Size: 828 5524
SacII CC_GC'GG
Cuts at: 3200 3200
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SbfI CC_TGCA'GG
Cuts at: 2670 3704 2670
Size: 1034 5318
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SfcI C'TryA_G
Cuts at: 826 1045 2563 2666 3353 3700 4835 5516 5707
Size: 219 1518 103 687 347 1135 681 191
Cuts at: 5707 826
Size: 1471
Fragments arranged by size:
1518 1471 1135 687 681 347 219 191
103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3023 4456 5357 5619 820
Size: 197 926 1080 1433 901 262 1553
Fragments arranged by size:
1553 1433 1080 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3710 1132
Size: 2578 3774
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 3118 1517
Size: 1601 4751
StyI C'CwwG_G
Cuts at: 513 1065 2004 2754 3404 3791 513
Size: 552 939 750 650 387 3074
Fragments arranged by size:
3074 939 750 650 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 3544 4379 4396 4549 4734 5179
Size: 1255 647 1317 835 17 153 185 445
Cuts at: 5179 325
Size: 1498
Fragments arranged by size:
1498 1317 1255 835 647 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2158 4598 6310
Size: 274 80 33 51 1443 181 2440 1712
Cuts at: 6310 96
Size: 138
Fragments arranged by size:
2440 1712 1443 274 181 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3235 3267 6312 6344 3235
Size: 32 3045 32 3243
Fragments arranged by size:
3243 3045 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3313 3313
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 4481 4481
Size: 6352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2949 4160 276
Size: 53 83 186 936 1415 1211 2468
Fragments arranged by size:
2468 1415 1211 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI PacI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI