Restriction map for
pFN21AE4137
(Circular) (Six-base) MAPSORT of: pFN21AE4137.seq Check: 2310 from: 1 to: 6061
pFN21AE4137 6061 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2441 2600 3223 2441
Size: 159 623 5279
Fragments arranged by size:
5279 623 159
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2248 3404 1937
Size: 311 1156 4594
Fragments arranged by size:
4594 1156 311
Acc65I G'GTAC_C
Cuts at: 2204 3382 2204
Size: 1178 4883
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 3117 4124 5508 5776 1632
Size: 641 844 1007 1384 268 1917
Fragments arranged by size:
1917 1384 1007 844 641 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 2731 4960 5937 1203
Size: 1528 2229 977 1327
Fragments arranged by size:
2229 1528 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 2492 1166
Size: 1326 4735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 3804 5376 3804
Size: 1572 4489
ApaLI G'TGCA_C
Cuts at: 2457 4118 5274 2457
Size: 1661 1156 3244
Fragments arranged by size:
3244 1661 1156
ApoI r'AATT_y
Cuts at: 1493 2799 3370 3596 3748 5857 1493
Size: 1306 571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1306 571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3386 1870
Size: 73 1443 4545
Fragments arranged by size:
4545 1443 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 3382 4837 618
Size: 325 217 627 417 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 417 325 217
BanII G_rGCy'C
Cuts at: 1391 2520 3141 3380 1391
Size: 1129 621 239 4072
Fragments arranged by size:
4072 1129 621 239
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2592 928
Size: 971 693 4397
Fragments arranged by size:
4397 971 693
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2217 2251 2584 2618 4252 4286 1903
Size: 34 280 34 333 34 1634 34 3678
Fragments arranged by size:
3678 1634 333 280 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 2571 2745 2761 1548
Size: 1023 174 16 4848
Fragments arranged by size:
4848 1023 174 16
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3170 4672 136
Size: 107 122 71 631 605 1498 1502 1525
Fragments arranged by size:
1525 1502 1498 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2461 3175 4122 5278 1790
Size: 671 714 947 1156 2573
Fragments arranged by size:
2573 1156 947 714 671
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3792 4750 442
Size: 416 53 83 399 24 2375 958 1753
Fragments arranged by size:
2375 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 2794 2031
Size: 763 5298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3174 3186 3248 3267 4721 1239
Size: 1935 12 62 19 1454 2579
Fragments arranged by size:
2579 1935 1454 62 19 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2969 3852 493
Size: 711 1765 883 2702
Fragments arranged by size:
2702 1765 883 711
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3048 3078 1178
Size: 30 165 30 1645 30 4161
Fragments arranged by size:
4161 1645 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 2405 2687 2732 3069 3246 1186
Size: 1028 191 282 45 337 177 4001
Fragments arranged by size:
4001 1028 337 282 191 177 45
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2557 3511 4272 5300 1540
Size: 275 186 556 954 761 1028 2301
Fragments arranged by size:
2301 1028 954 761 556 275 186
BsiHKAI G_wGCw'C
Cuts at: 2461 2520 2743 3141 4122 4207 5278 2461
Size: 59 223 398 981 85 1071 3244
Fragments arranged by size:
3244 1071 981 398 223 85 59
BsmI GAATG_Cn'
Cuts at: 2848 3572 3665 2848
Size: 724 93 5244
Fragments arranged by size:
5244 724 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2782 2782
Size: 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2461 2520 2743 3141 3175 3380 4122
Size: 399 671 59 223 398 34 205 742
Cuts at: 4122 4207 5278 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 671 399 398 223 205
85 59 34
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2441 2600 3223 3416 844
Size: 1463 134 159 623 193 3489
Fragments arranged by size:
3489 1463 623 193 159 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 2540 4121 5133 2540
Size: 1581 1012 3468
Fragments arranged by size:
3468 1581 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2542 2542
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056
Size: 1419 367 385 17 1452 86 12 346
Cuts at: 6056 1972
Size: 1977
Fragments arranged by size:
1977 1452 1419 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2052 2211 513
Size: 552 987 159 4363
Fragments arranged by size:
4363 987 552 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2515 487
Size: 683 56 1289 4033
Fragments arranged by size:
4033 1289 683 56
BtsI GCAGTG_nn'
Cuts at: 2835 3301 3577 4371 4391 2835
Size: 466 276 794 20 4505
Fragments arranged by size:
4505 794 466 276 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026
Size: 54 1425 50 275 18 1678 889 1629
Cuts at: 6026 8
Size: 43
Fragments arranged by size:
1678 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2382 3069 3991 1353
Size: 1029 687 922 3423
Fragments arranged by size:
3423 1029 922 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3033 4648 5022 5168 387
Size: 1064 138 1444 1615 374 146 1280
Fragments arranged by size:
1615 1444 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2518 3139 2518
Size: 621 5440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 3117 3174 3186 3248 3267 4124
Size: 393 641 844 57 12 62 19 857
Cuts at: 4124 4721 5508 5776 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 857 844 787 641 597 393 268
62 57 19 12
EcoRI G'AATT_C
Cuts at: 2799 3370 3748 2799
Size: 571 378 5112
Fragments arranged by size:
5112 571 378
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373
Size: 32 135 32 3130 32 42 32 2626
Fragments arranged by size:
3130 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2941 3405 3651 669
Size: 988 281 1003 464 246 3079
Fragments arranged by size:
3079 1003 988 464 281 246
HindIII A'AGCT_T
Cuts at: 748 3129 748
Size: 2381 3680
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 2208 3386 2208
Size: 1178 4883
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 5175 5359 1305
Size: 1080 2790 184 2007
Fragments arranged by size:
2790 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2540 2736 2913 4019 4378 4537
Size: 648 1326 48 196 177 1106 359 159
Cuts at: 4537 518
Size: 2042
Fragments arranged by size:
2042 1326 1106 648 359 196 177 159
48
MspA1I CmG'CkG
Cuts at: 1606 2235 2581 3477 4154 5302 5547 1606
Size: 629 346 896 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 677 629 346 245
NcoI C'CATG_G
Cuts at: 513 1065 2052 2211 513
Size: 552 987 159 4363
Fragments arranged by size:
4363 987 552 159
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2735 3419 4964 1132
Size: 97 1506 684 1545 2229
Fragments arranged by size:
2229 1545 1506 684 97
PasI CC'CwG_GG
Cuts at: 1348 3167 1348
Size: 1819 4242
PciI A'CATG_T
Cuts at: 2731 4960 2731
Size: 2229 3832
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3290 1108
Size: 2182 3879
PfoI T'CCnGG_A
Cuts at: 3103 3103
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2509 2696 1613
Size: 896 187 4978
Fragments arranged by size:
4978 896 187
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3072 3104 3072
Size: 32 6029
PstI C_TGCA'G
Cuts at: 830 2841 3159 3280 3413 830
Size: 2011 318 121 133 3478
Fragments arranged by size:
3478 2011 318 133 121
PvuI CG_AT'CG
Cuts at: 2001 2557 2001
Size: 556 5505
PvuII CAG'CTG
Cuts at: 2581 2581
Size: 6061
SacI G_AGCT'C
Cuts at: 2520 3141 2520
Size: 621 5440
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2245 2837 3155 3276 3409 4544 5225
Size: 219 1200 592 318 121 133 1135 681
Cuts at: 5225 5416 826
Size: 191 1471
Fragments arranged by size:
1471 1200 1135 681 592 318 219 191
133 121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 2174 1517
Size: 657 5404
StyI C'CwwG_G
Cuts at: 513 1065 2052 2211 3500 513
Size: 552 987 159 1289 3074
Fragments arranged by size:
3074 1289 987 552 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4307 6019 96
Size: 274 80 33 51 1443 2330 1712 138
Fragments arranged by size:
2330 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2930 3087 4190 2930
Size: 157 1103 4801
Fragments arranged by size:
4801 1103 157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI