Restriction map for
pFN21AE4136
(Circular) (Six-base) MAPSORT of: pFN21AE4136.seq Check: 5105 from: 1 to: 6346
pFN21AE4136 6346 bp
With 182 enzymes: SgfI *
March 28, 2012 13:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4156 278
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3003 3689 1937
Size: 1066 686 4594
Fragments arranged by size:
4594 1066 686
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 4473 4846 1563
Size: 2910 373 3063
Fragments arranged by size:
3063 2910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2207 3068 3465 4409 5793 6061 1632
Size: 416 159 861 397 944 1384 268 1917
Fragments arranged by size:
1917 1384 944 861 416 397 268 159
AflII C'TTAA_G
Cuts at: 820 1017 2755 820
Size: 197 1738 4411
Fragments arranged by size:
4411 1738 197
AflIII A'CryG_T
Cuts at: 1203 2937 5245 6222 1203
Size: 1734 2308 977 1327
Fragments arranged by size:
2308 1734 1327 977
AgeI A'CCGG_T
Cuts at: 2432 5177 2432
Size: 2745 3601
AhdI GACnn_n'nnGTC
Cuts at: 3107 5075 3107
Size: 1968 4378
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2217 1184
Size: 1033 5313
AlwNI CAG_nnn'CTG
Cuts at: 2570 2620 2998 4089 5661 2570
Size: 50 378 1091 1572 3255
Fragments arranged by size:
3255 1572 1091 378 50
ApaLI G'TGCA_C
Cuts at: 3387 4403 5559 3387
Size: 1016 1156 4174
Fragments arranged by size:
4174 1156 1016
ApoI r'AATT_y
Cuts at: 1493 2240 2634 2819 3655 3881 4033 6142 1493
Size: 747 394 185 836 226 152 2109 1697
Fragments arranged by size:
2109 1697 836 747 394 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 2532 3450 3603 3671 1870
Size: 73 589 918 153 68 4545
Fragments arranged by size:
4545 918 589 153 73 68
BamHI G'GATC_C
Cuts at: 2666 3676 4043 2666
Size: 1010 367 4969
Fragments arranged by size:
4969 1010 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3286 3667 5122 618
Size: 325 217 627 1499 381 1455 1842
Fragments arranged by size:
1842 1499 1455 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2119 2260 2523 2605 3665 1391
Size: 728 141 263 82 1060 4072
Fragments arranged by size:
4072 1060 728 263 141 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 928
Size: 971 702 4673
Fragments arranged by size:
4673 971 702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4537 4571 1903
Size: 34 2600 34 3678
Fragments arranged by size:
3678 2600 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 5448 4238
Size: 1210 5136
BclI T'GATC_A
Cuts at: 1548 2824 3220 1548
Size: 1276 396 4674
Fragments arranged by size:
4674 1276 396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4957 136
Size: 107 122 71 631 605 3285 1525
Fragments arranged by size:
3285 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 2586 3315 3763 2586
Size: 729 448 5169
Fragments arranged by size:
5169 729 448
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2051 3391 3603 4407 5563 1790
Size: 254 7 1340 212 804 1156 2573
Fragments arranged by size:
2573 1340 1156 804 254 212 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3277 4077 5035
Size: 416 53 83 399 24 1860 800 958
Cuts at: 5035 442
Size: 1753
Fragments arranged by size:
1860 1753 958 800 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 3056 5006 1239
Size: 959 858 1950 2579
Fragments arranged by size:
2579 1950 959 858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 3391 4471 5336 5634 2870
Size: 521 1080 865 298 3582
Fragments arranged by size:
3582 1080 865 521 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4137 493
Size: 711 2933 2702
Fragments arranged by size:
2933 2702 711
BsaBI GATnn'nnATC
Cuts at: 2853 6340 2853
Size: 3487 2859
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2554 3627 4153 4535
Size: 53 83 186 936 1021 1073 526 382
Cuts at: 4535 5170 275
Size: 635 1451
Fragments arranged by size:
1451 1073 1021 936 635 526 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2432 3571 4779 5177 5451 5598 6009 2432
Size: 1139 1208 398 274 147 411 2769
Fragments arranged by size:
2769 1208 1139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6121
Fragments arranged by size:
6121 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2873 3515 1186
Size: 941 746 642 4017
Fragments arranged by size:
4017 941 746 642
BseYI C'CCAG_C
Cuts at: 3211 5549 6045 3211
Size: 2338 496 3512
Fragments arranged by size:
3512 2338 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2583 2813 2862 1163
Size: 573 847 230 49 4647
Fragments arranged by size:
4647 847 573 230 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2137 3796 4557 5585 1540
Size: 275 186 136 1659 761 1028 2301
Fragments arranged by size:
2301 1659 1028 761 275 186 136
BsiHKAI G_wGCw'C
Cuts at: 2260 3391 4407 4492 5563 2260
Size: 1131 1016 85 1071 3043
Fragments arranged by size:
3043 1131 1071 1016 85
BsmI GAATG_Cn'
Cuts at: 2016 3857 3950 2016
Size: 1841 93 4412
Fragments arranged by size:
4412 1841 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2051 2119 2260 2523 2605 3391
Size: 399 254 7 68 141 263 82 786
Cuts at: 3391 3603 3665 4407 4492 5563 1391
Size: 212 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 786 742 399 263 254 212
141 85 82 68 62 7
BspEI T'CCGG_A
Cuts at: 3571 6009 3571
Size: 2438 3908
BspHI T'CATG_A
Cuts at: 4235 5965 4235
Size: 1730 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 3298 3701 844
Size: 1563 891 403 3489
Fragments arranged by size:
3489 1563 891 403
BsrBI CCG'CTC
Cuts at: 1394 1823 3554 4233 1394
Size: 429 1731 679 3507
Fragments arranged by size:
3507 1731 679 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3587 4841 5015 65
Size: 1183 2339 1254 174 1396
Fragments arranged by size:
2339 1396 1254 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2432 4990 5177 1700
Size: 110 622 2558 187 2869
Fragments arranged by size:
2869 2558 622 187 110
BsrGI T'GTAC_A
Cuts at: 96 2934 96
Size: 2838 3508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 2216 4406 5418 2216
Size: 2190 1012 3144
Fragments arranged by size:
3144 2190 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2620 2800 794
Size: 1826 180 4340
Fragments arranged by size:
4340 1826 180
BstBI TT'CG_AA
Cuts at: 5150 5150
Size: 6346
BstEII G'GTnAC_C
Cuts at: 1184 3557 1184
Size: 2373 3973
BstXI CCAn_nnnn'nTGG
Cuts at: 3139 3139
Size: 6346
BstYI r'GATC_y
Cuts at: 1972 2666 3155 3378 3676 4043 4428 4445 5897
Size: 694 489 223 298 367 385 17 1452
Cuts at: 5897 5983 5995 6341 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 694 489 385 367 346 298
223 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2548 513
Size: 552 939 544 4311
Fragments arranged by size:
4311 939 552 544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 2571 2769 3522 3862 4656 4676 2571
Size: 198 753 340 794 20 4241
Fragments arranged by size:
4241 794 753 340 198 20
ClaI AT'CG_AT
Cuts at: 1554 2156 1554
Size: 602 5744
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 2964 3650 2964
Size: 686 5660
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5353 809
Size: 507 4037 1802
Fragments arranged by size:
4037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2482 2650
Size: 54 1425 50 275 18 390 262 168
Cuts at: 2650 3793 4682 6311 8
Size: 1143 889 1629 43
Fragments arranged by size:
1629 1425 1143 889 390 275 262 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3793 1537
Size: 275 1981 4090
Fragments arranged by size:
4090 1981 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3284 3399 4276 1353
Size: 999 932 115 877 3423
Fragments arranged by size:
3423 999 932 877 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 2527 4933 5307 5453 387
Size: 1064 138 482 456 2406 374 146 1280
Fragments arranged by size:
2406 1280 1064 482 456 374 146 138
EcoICRI GAG'CTC
Cuts at: 2258 2258
Size: 6346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2198 2207 3056 3068 3465 4409
Size: 393 416 150 9 849 12 397 944
Cuts at: 4409 5006 5793 6061 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 944 849 787 597 416 397 393
268 150 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 3281 2357
Size: 924 5422
EcoO109I rG'GnC_Cy
Cuts at: 2362 3052 2362
Size: 690 5656
EcoRI G'AATT_C
Cuts at: 2240 3655 4033 2240
Size: 1415 378 4553
Fragments arranged by size:
4553 1415 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3495 3495
Size: 6346
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 2497 5493 1678
Size: 819 2996 2531
Fragments arranged by size:
2996 2531 819
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 2674 2706 3093
Size: 32 135 32 645 32 425 32 387
Cuts at: 3093 3125 3147 3179 4987 5019 5061 5093 1373
Size: 32 22 32 1808 32 42 32 2626
Fragments arranged by size:
2626 1808 645 425 387 135 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3690 3936 669
Size: 988 281 1752 246 3079
Fragments arranged by size:
3079 1752 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6346
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 2646 3087 3945 2646
Size: 441 858 5047
Fragments arranged by size:
5047 858 441
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5460 5644 1305
Size: 4155 184 2007
Fragments arranged by size:
4155 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 2652 10
Size: 54 1425 343 390 430 3704
Fragments arranged by size:
3704 1425 430 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3398 3540 4304 4663 4822 518
Size: 648 2232 142 764 359 159 2042
Fragments arranged by size:
2232 2042 764 648 359 159 142
MspA1I CmG'CkG
Cuts at: 1606 3509 3762 4439 5587 5832 1606
Size: 1903 253 677 1148 245 2120
Fragments arranged by size:
2120 1903 1148 677 253 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4855
Fragments arranged by size:
4855 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6346
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 2941 3704 5249 1132
Size: 97 1712 763 1545 2229
Fragments arranged by size:
2229 1712 1545 763 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 2937 5245 2937
Size: 2308 4038
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6346
PfoI T'CCnGG_A
Cuts at: 3197 3197
Size: 6346
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 6346
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PstI C_TGCA'G
Cuts at: 830 2616 2625 2994 3698 830
Size: 1786 9 369 704 3478
Fragments arranged by size:
3478 1786 704 369 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 3509 3509
Size: 6346
SacI G_AGCT'C
Cuts at: 2260 2260
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 4594 6306 4594
Size: 1712 4634
SfcI C'TryA_G
Cuts at: 826 1045 2612 2621 2990 3694 4829 5510 5701
Size: 219 1567 9 369 704 1135 681 191
Cuts at: 5701 826
Size: 1471
Fragments arranged by size:
1567 1471 1135 704 681 369 219 191
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 2534 3452 3605 3673 1872
Size: 662 918 153 68 4545
Fragments arranged by size:
4545 918 662 153 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2755 2885 3370 4450 5351 5613
Size: 197 926 812 130 485 1080 901 262
Cuts at: 5613 820
Size: 1553
Fragments arranged by size:
1553 1080 926 901 812 485 262 197
130
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3704 1132
Size: 2572 3774
SrfI GCCC'GGGC
Cuts at: 3452 3452
Size: 6346
SspI AAT'ATT
Cuts at: 5 52 4270 5
Size: 47 4218 2081
Fragments arranged by size:
4218 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6346
StyI C'CwwG_G
Cuts at: 513 1065 2004 2283 3144 3785 513
Size: 552 939 279 861 641 3074
Fragments arranged by size:
3074 939 861 641 552 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 4373 4390 4543 4728 5173 325
Size: 1255 417 2376 17 153 185 445 1498
Fragments arranged by size:
2376 1498 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2934 4592 6304
Size: 274 80 33 51 1443 957 1658 1712
Cuts at: 6304 96
Size: 138
Fragments arranged by size:
1712 1658 1443 957 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 6306 6338 2451
Size: 32 3823 32 2459
Fragments arranged by size:
3823 2459 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6346
XbaI T'CTAG_A
Cuts at: 3682 3682
Size: 6346
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6346
XmaI C'CCGG_G
Cuts at: 1870 2532 3450 3603 3671 1870
Size: 662 918 153 68 4545
Fragments arranged by size:
4545 918 662 153 68
XmnI GAAnn'nnTTC
Cuts at: 4475 4475
Size: 6346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4154 276
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI