Restriction map for
pFN21AE4135
(Circular) (Six-base) MAPSORT of: pFN21AE4135.seq Check: 9910 from: 1 to: 6334
pFN21AE4135 6334 bp
With 182 enzymes: SgfI *
March 28, 2012 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 2507 3408 3555 4397 5781 6049 1632
Size: 701 174 901 147 842 1384 268 1917
Fragments arranged by size:
1917 1384 901 842 701 268 174 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 2448 5233 6210 1203
Size: 1245 2785 977 1327
Fragments arranged by size:
2785 1327 1245 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 3047 1166
Size: 1881 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3113 1184
Size: 1929 4405
AlwNI CAG_nnn'CTG
Cuts at: 2374 3191 4077 5649 2374
Size: 817 886 1572 3059
Fragments arranged by size:
3059 1572 886 817
ApaLI G'TGCA_C
Cuts at: 2792 4391 5547 2792
Size: 1599 1156 3579
Fragments arranged by size:
3579 1599 1156
ApoI r'AATT_y
Cuts at: 1493 2014 2430 2624 3643 3869 4021 6130 1493
Size: 521 416 194 1019 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 521 416 226 194 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 3024 3059 3659 1870
Size: 73 1081 35 600 4545
Fragments arranged by size:
4545 1081 600 73 35
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3197 3655 5110 618
Size: 325 217 627 1410 458 1455 1842
Fragments arranged by size:
1842 1455 1410 627 458 325 217
BanII G_rGCy'C
Cuts at: 1391 2208 2565 3464 3653 1391
Size: 817 357 899 189 4072
Fragments arranged by size:
4072 899 817 357 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3505 928
Size: 971 1606 3757
Fragments arranged by size:
3757 1606 971
BbvCI CC'TCA_GC
Cuts at: 2415 2415
Size: 6334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4945 136
Size: 107 122 71 631 605 3273 1525
Fragments arranged by size:
3273 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3367 6329 3367
Size: 2962 3372
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 2796 3060 4395 5551 1790
Size: 1006 264 1335 1156 2573
Fragments arranged by size:
2573 1335 1156 1006 264
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3010 3092 4065
Size: 416 53 83 399 24 1593 82 973
Cuts at: 4065 5023 442
Size: 958 1753
Fragments arranged by size:
1753 1593 973 958 416 399 83 82
53 24
BmtI G_CTAG'C
Cuts at: 1056 2079 1056
Size: 1023 5311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 3020 3478 4994 1239
Size: 882 899 458 1516 2579
Fragments arranged by size:
2579 1516 899 882 458
Bpu10I CC'TnA_GC
Cuts at: 2415 2559 2415
Size: 144 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2014 2157 4459 5324 5622 2014
Size: 143 2302 865 298 2726
Fragments arranged by size:
2726 2302 865 298 143
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4997 882
Size: 617 3498 2219
Fragments arranged by size:
3498 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4125 493
Size: 711 2921 2702
Fragments arranged by size:
2921 2702 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 4523 5158 275
Size: 53 83 186 936 2608 382 635 1451
Fragments arranged by size:
2608 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6109
Fragments arranged by size:
6109 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2213 2216 2432 3251 1186
Size: 922 105 3 216 819 4269
Fragments arranged by size:
4269 922 819 216 105 3
BseYI C'CCAG_C
Cuts at: 2604 5537 6033 2604
Size: 2933 496 2905
Fragments arranged by size:
2933 2905 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2877 1163
Size: 573 1141 4620
Fragments arranged by size:
4620 1141 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2565 2796 3016 3464 4395 4480 5551 2208
Size: 357 231 220 448 931 85 1071 2991
Fragments arranged by size:
2991 1071 931 448 357 231 220 85
BsmI GAATG_Cn'
Cuts at: 2483 3845 3938 2483
Size: 1362 93 4879
Fragments arranged by size:
4879 1362 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2565 2796 3016 3060 3464 3653
Size: 399 418 357 231 220 44 404 189
Cuts at: 3653 4395 4480 5551 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 418 404 399 357 231
220 189 85 44
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 2181 4223 5953 2181
Size: 2042 1730 2562
Fragments arranged by size:
2562 2042 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3689 844
Size: 2845 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4221 1394
Size: 429 2398 3507
Fragments arranged by size:
3507 2398 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3030 4978 5165 1700
Size: 110 1220 1948 187 2869
Fragments arranged by size:
2869 1948 1220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 3477 2230
Size: 1247 5087
BstYI r'GATC_y
Cuts at: 1972 3367 3664 4031 4416 4433 5885 5971 5983
Size: 1395 297 367 385 17 1452 86 12
Cuts at: 5983 6329 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1395 385 367 346 297 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2020 3450 2020
Size: 1430 4904
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 2392 2963 513
Size: 552 939 105 283 571 3884
Fragments arranged by size:
3884 939 571 552 283 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2910 3095 3850 4644 4664 2910
Size: 185 755 794 20 4580
Fragments arranged by size:
4580 794 755 185 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2911 2946 529
Size: 35 2347 35 3917
Fragments arranged by size:
3917 2347 35 35
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DraIII CAC_nnn'GTG
Cuts at: 3086 3086
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2884 3630 3781
Size: 54 1425 50 275 18 1054 746 151
Cuts at: 3781 4670 6299 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1054 889 746 275 151 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4264 1353
Size: 2911 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2206 2563 3462 2206
Size: 357 899 5078
Fragments arranged by size:
5078 899 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2333 2507 3020 3408 3478 3555
Size: 393 489 212 174 513 388 70 77
Cuts at: 3555 4397 4994 5781 6049 1239
Size: 842 597 787 268 1524
Fragments arranged by size:
1524 842 787 597 513 489 393 388
268 212 174 77 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 3449 2315
Size: 1134 5200
EcoRI G'AATT_C
Cuts at: 2014 3643 4021 2014
Size: 1629 378 4327
Fragments arranged by size:
4327 1629 378
EcoRV GAT'ATC
Cuts at: 2659 2659
Size: 6334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2760 2760
Size: 6334
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 5481 1678
Size: 3803 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 2943 2975 3095
Size: 32 135 32 1076 32 263 32 120
Cuts at: 3095 3127 4975 5007 5049 5081 1373
Size: 32 1848 32 42 32 2626
Fragments arranged by size:
2626 1848 1076 263 135 120 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2998 3678 3924 669
Size: 988 281 1060 680 246 3079
Fragments arranged by size:
3079 1060 988 680 281 246
HindIII A'AGCT_T
Cuts at: 748 2767 3554 748
Size: 2019 787 3528
Fragments arranged by size:
3528 2019 787
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 3138 5448 5632 1305
Size: 1026 807 2310 184 2007
Fragments arranged by size:
2310 2007 1026 807 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2886 10
Size: 54 1425 343 1054 3458
Fragments arranged by size:
3458 1425 1054 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2228 3047 4292 4651 4810 518
Size: 648 843 219 819 1245 359 159 2042
Fragments arranged by size:
2042 1245 843 819 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2458 2608 3356 3492 3750 4427 5575
Size: 449 403 150 748 136 258 677 1148
Cuts at: 5575 5820 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 748 677 449 403 258 245
150 136
NcoI C'CATG_G
Cuts at: 513 1065 2004 2109 2392 513
Size: 552 939 105 283 4455
Fragments arranged by size:
4455 939 552 283 105
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 2075 1052
Size: 1023 5311
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2452 2974 3349 3692 5237 1132
Size: 97 1223 522 375 343 1545 2229
Fragments arranged by size:
2229 1545 1223 522 375 343 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 2448 5233 2448
Size: 2785 3549
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 2374 2836 1108
Size: 1152 114 462 4606
Fragments arranged by size:
4606 1152 462 114
PfoI T'CCnGG_A
Cuts at: 3516 3516
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PstI C_TGCA'G
Cuts at: 830 2346 2613 3686 830
Size: 1516 267 1073 3478
Fragments arranged by size:
3478 1516 1073 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2055 2458 2608 3356 3492 2055
Size: 403 150 748 136 4897
Fragments arranged by size:
4897 748 403 150 136
SacI G_AGCT'C
Cuts at: 2208 2565 3464 2208
Size: 357 899 5078
Fragments arranged by size:
5078 899 357
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SfcI C'TryA_G
Cuts at: 826 1045 2342 2609 3682 4817 5498 5689 826
Size: 219 1297 267 1073 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 1073 681 267 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3061 3661 1872
Size: 1189 600 4545
Fragments arranged by size:
4545 1189 600
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2029 2172 4438 5339 5601 820
Size: 197 926 86 143 2266 901 262 1553
Fragments arranged by size:
2266 1553 926 901 262 197 143 86
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3349 3692 1132
Size: 2217 343 3774
Fragments arranged by size:
3774 2217 343
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 3455 1517
Size: 1938 4396
StyI C'CwwG_G
Cuts at: 513 1065 2004 2109 2392 3773 513
Size: 552 939 105 283 1381 3074
Fragments arranged by size:
3074 1381 939 552 283 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2139 3074 3537 4361 4378 4531 4716
Size: 1255 559 935 463 824 17 153 185
Cuts at: 4716 5161 325
Size: 445 1498
Fragments arranged by size:
1498 1255 935 824 559 463 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4580 6292 96
Size: 274 80 33 51 1443 2603 1712 138
Fragments arranged by size:
2603 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6294 6326 6294
Size: 32 6302
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 2194 3670 2194
Size: 1476 4858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2970 3079 2596
Size: 374 109 5851
Fragments arranged by size:
5851 374 109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 3059 3659 1870
Size: 1189 600 4545
Fragments arranged by size:
4545 1189 600
XmnI GAAnn'nnTTC
Cuts at: 4463 4463
Size: 6334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI