Restriction map for
pFN21AE4132
(Circular) (Six-base) MAPSORT of: pFN21AE4132.seq Check: 920 from: 1 to: 6304
pFN21AE4132 6304 bp
With 182 enzymes: SgfI *
March 28, 2012 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2316 3070 3263 2316
Size: 754 193 5357
Fragments arranged by size:
5357 754 193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 3220 3625 3220
Size: 405 5899
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4367 5751 6019 1632
Size: 2735 1384 268 1917
Fragments arranged by size:
2735 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 2084 5033 2084
Size: 2949 3355
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2781 2817 1184
Size: 1597 36 4671
Fragments arranged by size:
4671 1597 36
AlwNI CAG_nnn'CTG
Cuts at: 2074 3062 4047 5619 2074
Size: 988 985 1572 2759
Fragments arranged by size:
2759 1572 988 985
ApaI G_GGCC'C
Cuts at: 2710 2710
Size: 6304
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 3613 3839 3991 6100 1493
Size: 2120 226 152 2109 1697
Fragments arranged by size:
2120 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 2691 2709 3225 3413 3513 3629
Size: 73 504 244 18 516 188 100 116
Cuts at: 3629 1870
Size: 4545
Fragments arranged by size:
4545 516 504 244 188 116 100 73
18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2028 2061 2028
Size: 33 6271
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3220 3349 3625 5080 618
Size: 325 217 627 1433 129 276 1455 1842
Fragments arranged by size:
1842 1455 1433 627 325 276 217 129
BanII G_rGCy'C
Cuts at: 1391 2171 2370 2388 2661 2710 2895 3042 3212
Size: 780 199 18 273 49 185 147 170
Cuts at: 3212 3623 1391
Size: 411 4072
Fragments arranged by size:
4072 780 411 273 199 185 170 147
49 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2998 928
Size: 971 1099 4234
Fragments arranged by size:
4234 1099 971
BbvCI CC'TCA_GC
Cuts at: 2213 2213
Size: 6304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3471 3505 4495 4529 1903
Size: 34 50 34 1450 34 990 34 3678
Fragments arranged by size:
3678 1450 990 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 2417 4915
Size: 107 122 71 631 605 688 57 2498
Cuts at: 4915 136
Size: 1525
Fragments arranged by size:
2498 1525 688 631 605 122 107 71
57
BglII A'GATC_T
Cuts at: 2563 6299 2563
Size: 3736 2568
BlpI GC'TnA_GC
Cuts at: 2364 3302 3721 2364
Size: 938 419 4947
Fragments arranged by size:
4947 938 419
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2635 2710 4365 5521 1790
Size: 249 596 75 1655 1156 2573
Fragments arranged by size:
2573 1655 1156 596 249 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6304
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2889 2917 4035
Size: 416 53 83 399 24 1472 28 1118
Cuts at: 4035 4993 442
Size: 958 1753
Fragments arranged by size:
1753 1472 1118 958 416 399 83 53
28 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2296 3070 4964 1239
Size: 1057 774 1894 2579
Fragments arranged by size:
2579 1894 1057 774
Bpu10I CC'TnA_GC
Cuts at: 2012 2213 3036 2012
Size: 201 823 5280
Fragments arranged by size:
5280 823 201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3206 4429 5294 5592 3206
Size: 1223 865 298 3918
Fragments arranged by size:
3918 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 3017 4967 882
Size: 617 945 573 1950 2219
Fragments arranged by size:
2219 1950 945 617 573
BsaAI yAC'GTr
Cuts at: 493 1204 3121 4095 493
Size: 711 1917 974 2702
Fragments arranged by size:
2702 1917 974 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4111 4493 5128 275
Size: 53 83 186 936 2578 382 635 1451
Fragments arranged by size:
2578 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2102 4737 5135 5409 5556 5967 2102
Size: 2635 398 274 147 411 2439
Fragments arranged by size:
2635 2439 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2843 2873 3098 3128 1178
Size: 30 165 30 1440 30 225 30 4354
Fragments arranged by size:
4354 1440 225 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2288 2414 2927 1186
Size: 886 216 126 513 4563
Fragments arranged by size:
4563 886 513 216 126
BseYI C'CCAG_C
Cuts at: 3280 3599 5507 6003 3280
Size: 319 1908 496 3581
Fragments arranged by size:
3581 1908 496 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2406 3048 3575 1163
Size: 573 670 642 527 3892
Fragments arranged by size:
3892 670 642 573 527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3754 4515 5543 1540
Size: 275 186 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2171 2370 2388 3042 3414 4365 4450 5521 2171
Size: 199 18 654 372 951 85 1071 2954
Fragments arranged by size:
2954 1071 951 654 372 199 85 18
BsmI GAATG_Cn'
Cuts at: 3815 3908 3815
Size: 93 6211
BsmBI CGTCTCn'nnnn_
Cuts at: 2092 2605 2092
Size: 513 5791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2171 2370 2388 2635 2661 2710
Size: 399 249 132 199 18 247 26 49
Cuts at: 2710 2895 3042 3212 3414 3623 4365 4450 5521
Size: 185 147 170 202 209 742 85 1071
Cuts at: 5521 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 249 247 209 202
199 185 170 147 132 85 49 26
18
BspEI T'CCGG_A
Cuts at: 2102 5967 2102
Size: 3865 2439
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 2316 3070 3263 3306 3604 3659 844
Size: 1238 234 754 193 43 298 55 3489
Fragments arranged by size:
3489 1238 754 298 234 193 55 43
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 3126 4191 1394
Size: 429 193 1110 1065 3507
Fragments arranged by size:
3507 1110 1065 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4973 65
Size: 1183 3551 174 1396
Fragments arranged by size:
3551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4948 5135 1700
Size: 110 3138 187 2869
Fragments arranged by size:
3138 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6304
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstXI CCAn_nnnn'nTGG
Cuts at: 2869 2869
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 2563 3634 4001 4386 4403 5855 5941 5953
Size: 591 1071 367 385 17 1452 86 12
Cuts at: 5953 6299 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1071 591 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2244 513
Size: 552 1179 4573
Fragments arranged by size:
4573 1179 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2497 487
Size: 683 56 1271 4294
Fragments arranged by size:
4294 1271 683 56
BtsI GCAGTG_nn'
Cuts at: 2703 3542 3820 4614 4634 2703
Size: 839 278 794 20 4373
Fragments arranged by size:
4373 839 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2458 5311 809
Size: 507 1142 2853 1802
Fragments arranged by size:
2853 1802 1142 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2123 2497 3145
Size: 54 1425 50 275 18 293 374 648
Cuts at: 3145 3232 3751 4640 6269 8
Size: 87 519 889 1629 43
Fragments arranged by size:
1629 1425 889 648 519 374 293 275
87 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2176 4234 1353
Size: 823 2058 3423
Fragments arranged by size:
3423 2058 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2264 4891 5265 5411 387
Size: 1064 138 675 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 675 374 146 138
EcoICRI GAG'CTC
Cuts at: 2169 2368 2386 3040 2169
Size: 199 18 654 5433
Fragments arranged by size:
5433 654 199 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2296 3070 4367 4964 5751 6019 1239
Size: 393 664 774 1297 597 787 268 1524
Fragments arranged by size:
1524 1297 787 774 664 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2071 2209 2473 3295 2071
Size: 138 264 822 5080
Fragments arranged by size:
5080 822 264 138
EcoRI G'AATT_C
Cuts at: 3613 3991 3613
Size: 378 5926
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 5451 1678
Size: 3773 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2548 2580 4945
Size: 32 135 32 498 32 446 32 2365
Cuts at: 4945 4977 5019 5051 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2365 498 446 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3648 3894 669
Size: 988 281 1710 246 3079
Fragments arranged by size:
3079 1710 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3304 748
Size: 2556 3748
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3224 3629 3224
Size: 405 5899
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 2886 5418 5602 1305
Size: 939 642 2532 184 2007
Fragments arranged by size:
2532 2007 939 642 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2125 3147 3234 10
Size: 54 1425 343 293 1022 87 3080
Fragments arranged by size:
3080 1425 1022 343 293 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3000 4262 4621 4780 518
Size: 648 1834 1262 359 159 2042
Fragments arranged by size:
2042 1834 1262 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2351 2578 2721 2761 3095 3720 4397 5545
Size: 745 227 143 40 334 625 677 1148
Cuts at: 5545 5790 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 745 677 625 334 245 227
143 40
NcoI C'CATG_G
Cuts at: 513 1065 2244 513
Size: 552 1179 4573
Fragments arranged by size:
4573 1179 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 2506 3662 5207 1132
Size: 97 1277 1156 1545 2229
Fragments arranged by size:
2229 1545 1277 1156 97
PasI CC'CwG_GG
Cuts at: 1348 2032 2258 2983 1348
Size: 684 226 725 4669
Fragments arranged by size:
4669 725 684 226
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2581 3154 3241 1108
Size: 1473 573 87 4171
Fragments arranged by size:
4171 1473 573 87
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 6304
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 3295 2071
Size: 1224 5080
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspOMI G'GGCC_C
Cuts at: 2706 2706
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2160 3396 3656 830
Size: 1330 1236 260 3478
Fragments arranged by size:
3478 1330 1236 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2351 2578 2761 3095 2351
Size: 227 183 334 5560
Fragments arranged by size:
5560 334 227 183
SacI G_AGCT'C
Cuts at: 2171 2370 2388 3042 2171
Size: 199 18 654 5433
Fragments arranged by size:
5433 654 199 18
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SapI GCTCTTCn'nnn_
Cuts at: 2176 2176
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SfcI C'TryA_G
Cuts at: 826 1045 2156 2316 2851 3392 3421 3652 4787
Size: 219 1111 160 535 541 29 231 1135
Cuts at: 4787 5468 5659 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 541 535 231 219
191 160 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 2711 3631 1872
Size: 839 920 4545
Fragments arranged by size:
4545 920 839
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3185 4408 5309 5571 820
Size: 197 926 1242 1223 901 262 1553
Fragments arranged by size:
1553 1242 1223 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SrfI GCCC'GGGC
Cuts at: 2711 2711
Size: 6304
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 2411 3314 3511 1517
Size: 894 903 197 4310
Fragments arranged by size:
4310 903 894 197
StyI C'CwwG_G
Cuts at: 513 1065 2244 2469 2952 3506 3743 513
Size: 552 1179 225 483 554 237 3074
Fragments arranged by size:
3074 1179 554 552 483 237 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 2318 2975 3242 3272 4331 4348
Size: 1255 501 237 657 267 30 1059 17
Cuts at: 4348 4501 4686 5131 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1059 657 501 445 267 237
185 153 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4550 6262 96
Size: 274 80 33 51 1443 2573 1712 138
Fragments arranged by size:
2573 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6264 6296 6264
Size: 32 6272
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 2709 3629 1870
Size: 839 920 4545
Fragments arranged by size:
4545 920 839
XmnI GAAnn'nnTTC
Cuts at: 4433 4433
Size: 6304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SwaI XcmI