Restriction map for
pFN21AE4126
(Circular) (Six-base) MAPSORT of: pFN21AE4126.seq Check: 3959 from: 1 to: 6256
pFN21AE4126 6256 bp
With 182 enzymes: SgfI *
March 28, 2012 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2713 3049 3109 2713
Size: 336 60 5860
Fragments arranged by size:
5860 336 60
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3599 1937
Size: 1662 4594
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2852 4319 5703 5971 1632
Size: 1220 1467 1384 268 1917
Fragments arranged by size:
1917 1467 1384 1220 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 2944 5087 2944
Size: 2143 4113
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 2020 2575 2632 2662 3424 3518 3999 5571 2020
Size: 555 57 30 762 94 481 1572 2705
Fragments arranged by size:
2705 1572 762 555 481 94 57 30
ApaI G_GGCC'C
Cuts at: 2235 3089 3099 3237 3537 2235
Size: 854 10 138 300 4954
Fragments arranged by size:
4954 854 300 138 10
ApaLI G'TGCA_C
Cuts at: 2353 4313 5469 2353
Size: 1960 1156 3140
Fragments arranged by size:
3140 1960 1156
ApoI r'AATT_y
Cuts at: 1493 3565 3791 3943 6052 1493
Size: 2072 226 152 2109 1697
Fragments arranged by size:
2109 2072 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2238 2643 3081 3479 3581 1870
Size: 73 204 91 405 438 398 102 4545
Fragments arranged by size:
4545 438 405 398 204 102 91 73
BamHI G'GATC_C
Cuts at: 2242 2703 3586 3953 2242
Size: 461 883 367 4545
Fragments arranged by size:
4545 883 461 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2890 2941 3150 3577
Size: 325 217 627 387 716 51 209 427
Cuts at: 3577 5032 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 716 627 427 387 325 217
209 51
BanII G_rGCy'C
Cuts at: 1391 2058 2163 2235 2316 2715 3089 3099 3114
Size: 667 105 72 81 399 374 10 15
Cuts at: 3114 3237 3279 3411 3537 3575 1391
Size: 123 42 132 126 38 4072
Fragments arranged by size:
4072 667 399 374 132 126 123 105
81 72 42 38 15 10
BbeI G_GCGC'C
Cuts at: 2178 2178
Size: 6256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2817 3460 928
Size: 971 918 643 3724
Fragments arranged by size:
3724 971 918 643
BbvCI CC'TCA_GC
Cuts at: 2201 2201
Size: 6256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2975 3009 4447 4481 1903
Size: 34 1038 34 1438 34 3678
Fragments arranged by size:
3678 1438 1038 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2465 2773 2782
Size: 107 122 71 631 605 793 308 9
Cuts at: 2782 4867 136
Size: 2085 1525
Fragments arranged by size:
2085 1525 793 631 605 308 122 107
71 9
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 2843 3551 3673 2843
Size: 708 122 5426
Fragments arranged by size:
5426 708 122
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2235 2357 2893 3089 3099 3133 3153
Size: 357 88 122 536 196 10 34 20
Cuts at: 3153 3237 3537 4317 5473 1790
Size: 84 300 780 1156 2573
Fragments arranged by size:
2573 1156 780 536 357 300 196 122
88 84 34 20 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2063 3169 3203
Size: 416 53 83 399 24 646 1106 34
Cuts at: 3203 3987 4945 442
Size: 784 958 1753
Fragments arranged by size:
1753 1106 958 784 646 416 399 83
53 34 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2234 2330 2459 2738 2747 3284 3425
Size: 803 192 96 129 279 9 537 141
Cuts at: 3425 4916 1239
Size: 1491 2579
Fragments arranged by size:
2579 1491 803 537 279 192 141 129
96 9
Bpu10I CC'TnA_GC
Cuts at: 2201 2201
Size: 6256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 4381 5246 5544 2393
Size: 1988 865 298 3105
Fragments arranged by size:
3105 1988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2548 4919 882
Size: 617 1049 2371 2219
Fragments arranged by size:
2371 2219 1049 617
BsaAI yAC'GTr
Cuts at: 493 1204 4047 493
Size: 711 2843 2702
Fragments arranged by size:
2843 2702 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 3198 4063 4445
Size: 53 83 186 936 642 1023 865 382
Cuts at: 4445 5080 275
Size: 635 1451
Fragments arranged by size:
1451 1023 936 865 642 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2944 4689 5087 5361 5508 5919 2944
Size: 1745 398 274 147 411 3281
Fragments arranged by size:
3281 1745 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6031
Fragments arranged by size:
6031 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2617 2666 2750 2852 2986 3467 1186
Size: 853 578 49 84 102 134 481 3975
Fragments arranged by size:
3975 853 578 481 134 102 84 49
BseYI C'CCAG_C
Cuts at: 2421 2448 2821 3229 3324 3417 5459 5955 2421
Size: 27 373 408 95 93 2042 496 2722
Fragments arranged by size:
2722 2042 496 408 373 95 93 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2365 3244 1163
Size: 573 629 879 4175
Fragments arranged by size:
4175 879 629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2306 3706 4467 5495 1540
Size: 275 186 305 1400 761 1028 2301
Fragments arranged by size:
2301 1400 1028 761 305 275 186
BsiHKAI G_wGCw'C
Cuts at: 2316 2357 2715 3114 3552 4317 4402 5473 2316
Size: 41 358 399 438 765 85 1071 3099
Fragments arranged by size:
3099 1071 765 438 399 358 85 41
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
BsmBI CGTCTCn'nnnn_
Cuts at: 2640 3205 2640
Size: 565 5691
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2147 2163 2235 2316 2357 2715
Size: 399 268 89 16 72 81 41 358
Cuts at: 2715 2893 3089 3099 3114 3133 3153 3237 3279
Size: 178 196 10 15 19 20 84 42
Cuts at: 3279 3411 3537 3552 3575 4317 4402 5473 1391
Size: 132 126 15 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 268 196 178
132 126 89 85 84 81 72 42
41 23 20 19 16 15 15 10
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 2713 3049 3109 3611 844
Size: 1201 668 336 60 502 3489
Fragments arranged by size:
3489 1201 668 502 336 60
BsrBI CCG'CTC
Cuts at: 1394 1823 2383 4143 1394
Size: 429 560 1760 3507
Fragments arranged by size:
3507 1760 560 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3131 4751 4925 65
Size: 1183 1883 1620 174 1396
Fragments arranged by size:
1883 1620 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2847 2944 4900 5087 1700
Size: 110 1037 97 1956 187 2869
Fragments arranged by size:
2869 1956 1037 187 110 97
BsrGI T'GTAC_A
Cuts at: 96 3426 96
Size: 3330 2926
BssHII G'CGCG_C
Cuts at: 1925 2224 2282 3169 1925
Size: 299 58 887 5012
Fragments arranged by size:
5012 887 299 58
BssSI C'ACGA_G
Cuts at: 2378 4316 5328 2378
Size: 1938 1012 3306
Fragments arranged by size:
3306 1938 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2352 2662 794
Size: 1558 310 4388
Fragments arranged by size:
4388 1558 310
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2443 2825 1184
Size: 1259 382 4615
Fragments arranged by size:
4615 1259 382
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2905 2050
Size: 855 5401
BstYI r'GATC_y
Cuts at: 1972 2242 2508 2703 3586 3953 4338 4355 5807
Size: 270 266 195 883 367 385 17 1452
Cuts at: 5807 5893 5905 6251 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 883 385 367 346 270 266
195 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3379 3379
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 2004 2296 2692 2919 3218 513
Size: 552 939 292 396 227 299 3551
Fragments arranged by size:
3551 939 552 396 299 292 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 2807 3772 4566 4586 2807
Size: 965 794 20 4477
Fragments arranged by size:
4477 965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DraIII CAC_nnn'GTG
Cuts at: 3187 3187
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2086 2303
Size: 54 1425 50 275 18 177 79 217
Cuts at: 2303 2616 3253 3703 4592 6221 8
Size: 313 637 450 889 1629 43
Fragments arranged by size:
1629 1425 889 637 450 313 275 217
177 79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2303 3703 1537
Size: 275 491 1400 4090
Fragments arranged by size:
4090 1400 491 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 2865 3449 3537 4186 1353
Size: 968 544 584 88 649 3423
Fragments arranged by size:
3423 968 649 584 544 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2314 2713 3112 2314
Size: 399 399 5458
Fragments arranged by size:
5458 399 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2234 2330 2459 2738 2747 2852
Size: 393 410 192 96 129 279 9 105
Cuts at: 2852 3284 3425 4319 4916 5703 5971 1239
Size: 432 141 894 597 787 268 1524
Fragments arranged by size:
1524 894 787 597 432 410 393 279
268 192 141 129 105 96 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2567 2720 2567
Size: 153 6103
EcoO109I rG'GnC_Cy
Cuts at: 2065 2103 2572 2774 2926 2933 3085 3233 3234
Size: 38 469 202 152 7 152 148 1
Cuts at: 3234 3260 3489 3533 3534 2065
Size: 26 229 44 1 4787
Fragments arranged by size:
4787 469 229 202 152 152 148 44
38 26 7 1 1
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FspI TGC'GCA
Cuts at: 2492 4762 2492
Size: 2270 3986
HaeII r_GCGC'y
Cuts at: 1678 2178 2390 2407 2633 3024 3331 5403 1678
Size: 500 212 17 226 391 307 2072 2531
Fragments arranged by size:
2531 2072 500 391 307 226 212 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2995 3027 3362 3394 4897
Size: 32 135 32 1423 32 335 32 1503
Cuts at: 4897 4929 4971 5003 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1503 1423 335 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2174 2174
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 10
Size: 54 1425 343 786 3648
Fragments arranged by size:
3648 1425 786 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 4214 4573 4732 518
Size: 648 1446 1602 359 159 2042
Fragments arranged by size:
2042 1602 1446 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2130 2205 2308 2337 2425 2659 2821
Size: 411 113 75 103 29 88 234 162
Cuts at: 2821 3672 4349 5497 5742 1606
Size: 851 677 1148 245 2120
Fragments arranged by size:
2120 1148 851 677 411 245 234 162
113 103 88 75 29
NaeI GCC'GGC
Cuts at: 2849 2849
Size: 6256
NarI GG'CG_CC
Cuts at: 2175 2175
Size: 6256
NcoI C'CATG_G
Cuts at: 513 1065 2004 2692 513
Size: 552 939 688 4077
Fragments arranged by size:
4077 939 688 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 2847 2847
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 2303 3703 2303
Size: 1400 4856
NspI r_CATG'y
Cuts at: 1132 1229 3614 5159 1132
Size: 97 2385 1545 2229
Fragments arranged by size:
2385 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2771 2885 3335 1348
Size: 1423 114 450 4269
Fragments arranged by size:
4269 1423 450 114
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2821 1108
Size: 1713 4543
PfoI T'CCnGG_A
Cuts at: 2112 2706 2715 3285 2112
Size: 594 9 570 5083
Fragments arranged by size:
5083 594 570 9
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2933 3260 3489 2065
Size: 868 327 229 4832
Fragments arranged by size:
4832 868 327 229
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 2231 3085 3095 3233 3533 2231
Size: 854 10 138 300 4954
Fragments arranged by size:
4954 854 300 138 10
PspXI vC'TCGA_Gb
Cuts at: 3479 3479
Size: 6256
PstI C_TGCA'G
Cuts at: 830 2702 2748 2802 3608 830
Size: 1872 46 54 806 3478
Fragments arranged by size:
3478 1872 806 54 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2017 2425 2821 2017
Size: 408 396 5452
Fragments arranged by size:
5452 408 396
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 6256
SacI G_AGCT'C
Cuts at: 2316 2715 3114 2316
Size: 399 399 5458
Fragments arranged by size:
5458 399 399
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SanDI GG'GwC_CC
Cuts at: 3260 3260
Size: 6256
SapI GCTCTTCn'nnn_
Cuts at: 2321 2321
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 2748 3608 2748
Size: 860 5396
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SfcI C'TryA_G
Cuts at: 826 1045 2567 2698 2744 2798 3397 3604 4739
Size: 219 1522 131 46 54 599 207 1135
Cuts at: 4739 5420 5611 826
Size: 681 191 1471
Fragments arranged by size:
1522 1471 1135 681 599 219 207 191
131 54 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 2465 2782 2465
Size: 317 5939
SfoI GGC'GCC
Cuts at: 2176 2176
Size: 6256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2240 3083 3583 1872
Size: 368 843 500 4545
Fragments arranged by size:
4545 843 500 368
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2643 3479 4360 5261 5523
Size: 197 926 465 235 836 881 901 262
Cuts at: 5523 820
Size: 1553
Fragments arranged by size:
1553 926 901 881 836 465 262 235
197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 3305 1517
Size: 1788 4468
StyI C'CwwG_G
Cuts at: 513 1065 2004 2692 3695 513
Size: 552 939 688 1003 3074
Fragments arranged by size:
3074 1003 939 688 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 2477 2813 4283 4300 4453 4638
Size: 1255 762 135 336 1470 17 153 185
Cuts at: 4638 5083 325
Size: 445 1498
Fragments arranged by size:
1498 1470 1255 762 445 336 185 153
135 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3426 4502 6214
Size: 274 80 33 51 1443 1449 1076 1712
Cuts at: 6214 96
Size: 138
Fragments arranged by size:
1712 1449 1443 1076 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2947 2979 6216 6248 2947
Size: 32 3237 32 2955
Fragments arranged by size:
3237 2955 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 2643 3479 1943
Size: 700 836 4720
Fragments arranged by size:
4720 836 700
XmaI C'CCGG_G
Cuts at: 1870 2238 3081 3581 1870
Size: 368 843 500 4545
Fragments arranged by size:
4545 843 500 368
XmnI GAAnn'nnTTC
Cuts at: 4385 4385
Size: 6256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PsrI SacII SexAI SrfI SwaI XcmI