Restriction map for
pFN21AE4124
(Circular) (Six-base) MAPSORT of: pFN21AE4124.seq Check: 1885 from: 1 to: 6241
pFN21AE4124 6241 bp
With 182 enzymes: SgfI *
March 28, 2012 13:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2913 4051 278
Size: 53 83 186 936 1377 1138 2468
Fragments arranged by size:
2468 1377 1138 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4304 5688 5956 1632
Size: 2672 1384 268 1917
Fragments arranged by size:
2672 1917 1384 268
AfeI AGC'GCT
Cuts at: 3254 3471 3254
Size: 217 6024
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 5140 6117 1203
Size: 3937 977 1327
Fragments arranged by size:
3937 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 2276 4970 2276
Size: 2694 3547
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2609 2993 1184
Size: 1425 384 4432
Fragments arranged by size:
4432 1425 384
AlwNI CAG_nnn'CTG
Cuts at: 2300 2999 3984 5556 2300
Size: 699 985 1572 2985
Fragments arranged by size:
2985 1572 985 699
ApaI G_GGCC'C
Cuts at: 3152 3235 3152
Size: 83 6158
ApaLI G'TGCA_C
Cuts at: 4298 5454 4298
Size: 1156 5085
ApoI r'AATT_y
Cuts at: 1493 3550 3776 3928 6037 1493
Size: 2057 226 152 2109 1697
Fragments arranged by size:
2109 2057 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 2266 3176 3317 3566 1870
Size: 73 323 910 141 249 4545
Fragments arranged by size:
4545 910 323 249 141 73
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2714 3209 3562 5017 618
Size: 325 217 627 927 495 353 1455 1842
Fragments arranged by size:
1842 1455 927 627 495 353 325 217
BanII G_rGCy'C
Cuts at: 1391 2187 3152 3176 3196 3235 3560 1391
Size: 796 965 24 20 39 325 4072
Fragments arranged by size:
4072 965 796 325 39 24 20
BbeI G_GCGC'C
Cuts at: 3213 3213
Size: 6241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5270
BbvCI CC'TCA_GC
Cuts at: 2546 2546
Size: 6241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2588 2622 3170 3204 4432
Size: 34 50 34 567 34 548 34 1228
Cuts at: 4432 4466 1903
Size: 34 3678
Fragments arranged by size:
3678 1228 567 548 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3051 4852 136
Size: 107 122 71 631 605 1379 1801 1525
Fragments arranged by size:
1801 1525 1379 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2833 6236 2833
Size: 3403 2838
BlpI GC'TnA_GC
Cuts at: 3658 3658
Size: 6241
Bme1580I G_kGCm'C
Cuts at: 1790 2575 3152 3235 3269 4302 5458 1790
Size: 785 577 83 34 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 785 577 83 34
BmgBI CAC'GTC
Cuts at: 1318 2338 1318
Size: 1020 5221
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3972 4930 442
Size: 416 53 83 399 24 2555 958 1753
Fragments arranged by size:
2555 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2172 2204 2172
Size: 32 6209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2353 3009 3269 3277 3470 4901 1239
Size: 903 211 656 260 8 193 1431 2579
Fragments arranged by size:
2579 1431 903 656 260 211 193 8
Bpu10I CC'TnA_GC
Cuts at: 2546 2546
Size: 6241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2741 4366 5231 5529 2240
Size: 501 1625 865 298 2952
Fragments arranged by size:
2952 1625 865 501 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2160 2469 2780 4904 882
Size: 617 661 309 311 2124 2219
Fragments arranged by size:
2219 2124 661 617 311 309
BsaAI yAC'GTr
Cuts at: 493 1204 3184 3264 4032 493
Size: 711 1980 80 768 2702
Fragments arranged by size:
2702 1980 768 711 80
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2910 3210 4048 4430
Size: 53 83 186 936 1377 300 838 382
Cuts at: 4430 5065 275
Size: 635 1451
Fragments arranged by size:
1451 1377 936 838 635 382 300 186
83 53
BsaWI w'CCGG_w
Cuts at: 4674 5072 5346 5493 5904 4674
Size: 398 274 147 411 5011
Fragments arranged by size:
5011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2095 2125 2742 2772 1178
Size: 30 165 30 692 30 617 30 4647
Fragments arranged by size:
4647 692 617 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2100 2103 2261 2495 2729 2798 3335 3367
Size: 914 3 158 234 234 69 537 32
Cuts at: 3367 1186
Size: 4060
Fragments arranged by size:
4060 914 537 234 234 158 69 32
3
BseYI C'CCAG_C
Cuts at: 2231 2330 3151 5444 5940 2231
Size: 99 821 2293 496 2532
Fragments arranged by size:
2532 2293 821 496 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2579 2989 3017 3116 3329 1163
Size: 573 843 410 28 99 213 4075
Fragments arranged by size:
4075 843 573 410 213 99 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 2452 3142 3183 3263 3537 4302 4387 5458
Size: 199 690 41 80 274 765 85 1071
Cuts at: 5458 2253
Size: 3036
Fragments arranged by size:
3036 1071 765 690 274 199 85 80
41
BsmI GAATG_Cn'
Cuts at: 3752 3845 3752
Size: 93 6148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2253 2452 2575 3142 3152 3176
Size: 399 397 66 199 123 567 10 24
Cuts at: 3176 3183 3196 3235 3263 3269 3537 3560 4302
Size: 7 13 39 28 6 268 23 742
Cuts at: 4302 4387 5458 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 397 268 199
123 85 66 39 28 24 23 13
10 7 6
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 2145 4130 5860 2145
Size: 1985 1730 2526
Fragments arranged by size:
2526 1985 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2308 3519 3596 844
Size: 1464 1211 77 3489
Fragments arranged by size:
3489 1464 1211 77
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 4128 1394
Size: 429 1618 687 3507
Fragments arranged by size:
3507 1618 687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2878 4736 4910 65
Size: 1183 1630 1858 174 1396
Fragments arranged by size:
1858 1630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4885 5072 1700
Size: 110 3075 187 2869
Fragments arranged by size:
3075 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 2597 4301 5313 2597
Size: 1704 1012 3525
Fragments arranged by size:
3525 1704 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 794
Size: 1529 4712
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 2221 2421 3106 3247 2221
Size: 200 685 141 5215
Fragments arranged by size:
5215 685 200 141
BstYI r'GATC_y
Cuts at: 1972 2833 3571 3938 4323 4340 5792 5878 5890
Size: 861 738 367 385 17 1452 86 12
Cuts at: 5890 6236 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 861 738 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2293 2741 2293
Size: 448 5793
BtgI C'CryG_G
Cuts at: 513 1065 2515 3049 3105 3205 513
Size: 552 1450 534 56 100 3549
Fragments arranged by size:
3549 1450 552 534 100 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2434 2650 487
Size: 683 56 1208 216 4078
Fragments arranged by size:
4078 1208 683 216 56
BtsI GCAGTG_nn'
Cuts at: 2328 2495 2619 2891 3757 4551 4571 2328
Size: 167 124 272 866 794 20 3998
Fragments arranged by size:
3998 866 794 272 167 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2518 2802
Size: 54 1425 50 275 18 181 507 284
Cuts at: 2802 2815 3052 3224 3688 4577 6206 8
Size: 13 237 172 464 889 1629 43
Fragments arranged by size:
1629 1425 889 507 464 284 275 237
181 172 54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2472 2775 3389 4171 1353
Size: 804 315 303 614 782 3423
Fragments arranged by size:
3423 804 782 614 315 303
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4828 5202 5348 387
Size: 1064 138 3239 374 146 1280
Fragments arranged by size:
3239 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2353 3009 3269 3277 3470 4304
Size: 393 510 211 656 260 8 193 834
Cuts at: 4304 4901 5688 5956 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 834 787 656 597 510 393 268
260 211 193 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 3239 3394 2597
Size: 642 155 5444
Fragments arranged by size:
5444 642 155
EcoO109I rG'GnC_Cy
Cuts at: 2043 2270 2297 2708 3148 3231 3232 3283 2043
Size: 227 27 411 440 83 1 51 5001
Fragments arranged by size:
5001 440 411 227 83 51 27 1
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 3099 3213 3256 3473 5388 1678
Size: 1421 114 43 217 1915 2531
Fragments arranged by size:
2531 1915 1421 217 114 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2190 2222 2262 2294 2753
Size: 32 135 32 618 32 40 32 459
Cuts at: 2753 2785 4882 4914 4956 4988 1373
Size: 32 2097 32 42 32 2626
Fragments arranged by size:
2626 2097 618 459 135 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3585 3831 669
Size: 988 281 1647 246 3079
Fragments arranged by size:
3079 1647 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KasI G'GCGC_C
Cuts at: 3209 3209
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5355 5539 1305
Size: 4050 184 2007
Fragments arranged by size:
4050 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2013 2520 2817 3054 3226
Size: 54 1425 343 181 507 297 237 172
Cuts at: 3226 10
Size: 3025
Fragments arranged by size:
3025 1425 507 343 297 237 181 172
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 4199 4558 4717 518
Size: 648 978 2055 359 159 2042
Fragments arranged by size:
2055 2042 978 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2916 2955 2965 2987 3062 3155 3429 3517
Size: 1310 39 10 22 75 93 274 88
Cuts at: 3517 3657 4334 5482 5727 1606
Size: 140 677 1148 245 2120
Fragments arranged by size:
2120 1310 1148 677 274 245 140 93
88 75 39 22 10
NarI GG'CG_CC
Cuts at: 3210 3210
Size: 6241
NcoI C'CATG_G
Cuts at: 513 1065 3105 3205 513
Size: 552 2040 100 3549
Fragments arranged by size:
3549 2040 552 100
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 2143 2504 2801 2859 3599 5144 1132
Size: 97 914 361 297 58 740 1545 2229
Fragments arranged by size:
2229 1545 914 740 361 297 97 58
PasI CC'CwG_GG
Cuts at: 1348 2576 1348
Size: 1228 5013
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6241
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PmlI CAC'GTG
Cuts at: 3184 3264 3184
Size: 80 6161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2270 2297 3283 2270
Size: 27 986 5228
Fragments arranged by size:
5228 986 27
PshAI GACnn'nnGTC
Cuts at: 1613 2461 1613
Size: 848 5393
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PspOMI G'GGCC_C
Cuts at: 3148 3231 3148
Size: 83 6158
PstI C_TGCA'G
Cuts at: 830 2614 2964 3147 3160 3357 3417 3516 3593
Size: 1784 350 183 13 197 60 99 77
Cuts at: 3593 830
Size: 3478
Fragments arranged by size:
3478 1784 350 197 183 99 77 60
13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2965 2987 3062 3155 3517 2965
Size: 22 75 93 362 5689
Fragments arranged by size:
5689 362 93 75 22
RsrII CG'GwC_CG
Cuts at: 2769 2769
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SanDI GG'GwC_CC
Cuts at: 2270 2270
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 2374 4489 6201 2374
Size: 2115 1712 2414
Fragments arranged by size:
2414 2115 1712
SfcI C'TryA_G
Cuts at: 826 1045 2610 2960 3143 3156 3298 3353 3413
Size: 219 1565 350 183 13 142 55 60
Cuts at: 3413 3512 3589 4724 5405 5596 826
Size: 99 77 1135 681 191 1471
Fragments arranged by size:
1565 1471 1135 681 350 219 191 183
142 99 77 60 55 13
SfoI GGC'GCC
Cuts at: 3211 3211
Size: 6241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 2268 3568 1872
Size: 396 1300 4545
Fragments arranged by size:
4545 1300 396
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 2720 4345 5246 5508 820
Size: 197 926 312 465 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 465 312 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 2603 1517
Size: 1086 5155
StyI C'CwwG_G
Cuts at: 513 1065 3105 3205 3240 3390 3680 513
Size: 552 2040 100 35 150 290 3074
Fragments arranged by size:
3074 2040 552 290 150 100 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2757 4268 4285 4438 4623 5068 325
Size: 1255 1177 1511 17 153 185 445 1498
Fragments arranged by size:
1511 1498 1255 1177 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2372 4487 6199
Size: 274 80 33 51 1443 395 2115 1712
Cuts at: 6199 96
Size: 138
Fragments arranged by size:
2115 1712 1443 395 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2742 2774 6201 6233 2742
Size: 32 3427 32 2750
Fragments arranged by size:
3427 2750 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6241
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2377
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 2266 3566 1870
Size: 396 1300 4545
Fragments arranged by size:
4545 1300 396
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2911 4049 276
Size: 53 83 186 936 1377 1138 2468
Fragments arranged by size:
2468 1377 1138 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI SacI
SacII SapI SexAI SfiI SrfI SwaI