Restriction map for
pFN21AE4118
(Circular) (Six-base) MAPSORT of: pFN21AE4118.seq Check: 3683 from: 1 to: 6193
pFN21AE4118 6193 bp
With 182 enzymes: SgfI *
March 28, 2012 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2220 2968 2220
Size: 748 5445
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2016 4003 278
Size: 53 83 186 936 480 1987 2468
Fragments arranged by size:
2468 1987 936 480 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3491 4256 5640 5908 1632
Size: 1859 765 1384 268 1917
Fragments arranged by size:
1917 1859 1384 765 268
AfeI AGC'GCT
Cuts at: 2145 2145
Size: 6193
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 5092 6069 1203
Size: 3889 977 1327
Fragments arranged by size:
3889 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 2079 2310 2675 1166
Size: 913 231 365 4684
Fragments arranged by size:
4684 913 365 231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaI G_GGCC'C
Cuts at: 2481 2525 2768 2817 2894 3068 2481
Size: 44 243 49 77 174 5606
Fragments arranged by size:
5606 243 174 77 49 44
ApaLI G'TGCA_C
Cuts at: 3308 4250 5406 3308
Size: 942 1156 4095
Fragments arranged by size:
4095 1156 942
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2772 2772
Size: 6193
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 2767 2816 2918 2933 3061 3183
Size: 73 582 242 49 102 15 128 122
Cuts at: 3183 3518 1870
Size: 335 4545
Fragments arranged by size:
4545 582 335 242 128 122 102 73
49 15
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2612 2834 2950 3398
Size: 325 217 627 334 491 222 116 448
Cuts at: 3398 3465 3514 4969 618
Size: 67 49 1455 1842
Fragments arranged by size:
1842 1455 627 491 448 334 325 222
217 116 67 49
BanII G_rGCy'C
Cuts at: 1391 2346 2481 2501 2525 2768 2817 2894 2979
Size: 955 135 20 24 243 49 77 85
Cuts at: 2979 3068 3100 3512 1391
Size: 89 32 412 4072
Fragments arranged by size:
4072 955 412 243 135 89 85 77
49 32 24 20
BbeI G_GCGC'C
Cuts at: 2125 2616 2954 2125
Size: 491 338 5364
Fragments arranged by size:
5364 491 338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2991 928
Size: 971 1092 4130
Fragments arranged by size:
4130 1092 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2660 4804 136
Size: 107 122 71 631 605 988 2144 1525
Fragments arranged by size:
2144 1525 988 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3240 6188 3240
Size: 2948 3245
BlpI GC'TnA_GC
Cuts at: 3449 3610 3449
Size: 161 6032
Bme1580I G_kGCm'C
Cuts at: 1790 2481 2525 2696 2768 2817 2894 2913 3068
Size: 691 44 171 72 49 77 19 155
Cuts at: 3068 3312 4254 5410 1790
Size: 244 942 1156 2573
Fragments arranged by size:
2573 1156 942 691 244 171 155 77
72 49 44 19
BmgBI CAC'GTC
Cuts at: 1318 2677 1318
Size: 1359 4834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 3924 4882
Size: 416 53 83 399 24 1251 1256 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1256 1251 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2347 2753 3071 3311 3359 3445 4853 1239
Size: 1108 406 318 240 48 86 1408 2579
Fragments arranged by size:
2579 1408 1108 406 318 240 86 48
Bpu10I CC'TnA_GC
Cuts at: 2500 2500
Size: 6193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 2366 2464 3359 4318 5183 5481 2132
Size: 234 98 895 959 865 298 2844
Fragments arranged by size:
2844 959 895 865 298 234 98
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3279 4856 882
Size: 617 1780 1577 2219
Fragments arranged by size:
2219 1780 1577 617
BsaAI yAC'GTr
Cuts at: 493 1204 3301 3984 493
Size: 711 2097 683 2702
Fragments arranged by size:
2702 2097 711 683
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 2122 2613 2951
Size: 53 83 186 936 480 109 491 338
Cuts at: 2951 3200 4000 4382 5017 275
Size: 249 800 382 635 1451
Fragments arranged by size:
1451 936 800 635 491 480 382 338
249 186 109 83 53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2344 2850 3140 3179 1186
Size: 1158 506 290 39 4200
Fragments arranged by size:
4200 1158 506 290 39
BseYI C'CCAG_C
Cuts at: 2553 2779 5396 5892 2553
Size: 226 2617 496 2854
Fragments arranged by size:
2854 2617 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2016 2273 2940 3377 3461 1163
Size: 573 280 257 667 437 84 3895
Fragments arranged by size:
3895 667 573 437 280 257 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2583 2641 2970 3030 3177 3229
Size: 275 186 582 58 329 60 147 52
Cuts at: 3229 3643 4404 5432 1540
Size: 414 761 1028 2301
Fragments arranged by size:
2301 1028 761 582 414 329 275 186
147 60 58 52
BsiHKAI G_wGCw'C
Cuts at: 2346 3132 3312 4254 4339 5410 2346
Size: 786 180 942 85 1071 3129
Fragments arranged by size:
3129 1071 942 786 180 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
BsmBI CGTCTCn'nnnn_
Cuts at: 2813 2813
Size: 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2481 2501 2525 2696 2768 2817
Size: 399 556 135 20 24 171 72 49
Cuts at: 2817 2894 2913 2979 3068 3100 3132 3312 3512
Size: 77 19 66 89 32 32 180 200
Cuts at: 3512 4254 4339 5410 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 200 180 171
135 89 85 77 72 66 49 32
32 24 20 19
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2220 2766 2968 3548 844
Size: 1280 96 546 202 580 3489
Fragments arranged by size:
3489 1280 580 546 202 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2109 2233 2737 3276 3295 3406 4080
Size: 429 286 124 504 539 19 111 674
Cuts at: 4080 1394
Size: 3507
Fragments arranged by size:
3507 674 539 504 429 286 124 111
19
BsrDI GCAATG_nn'
Cuts at: 65 1248 2476 4688 4862 65
Size: 1183 1228 2212 174 1396
Fragments arranged by size:
2212 1396 1228 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2279 2507 3132 3196 3384 4837 5024
Size: 110 469 228 625 64 188 1453 187
Cuts at: 5024 1700
Size: 2869
Fragments arranged by size:
2869 1453 625 469 228 188 187 110
64
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 2619 2772 1925
Size: 694 153 5346
Fragments arranged by size:
5346 694 153
BssSI C'ACGA_G
Cuts at: 3362 4253 5265 3362
Size: 891 1012 4290
Fragments arranged by size:
4290 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6193
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 2396 3049 2396
Size: 653 5540
BstYI r'GATC_y
Cuts at: 1972 3240 3523 3890 4275 4292 5744 5830 5842
Size: 1268 283 367 385 17 1452 86 12
Cuts at: 5842 6188 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1268 385 367 346 283 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2395 2583 2635 2796 2895 3030
Size: 552 939 391 188 52 161 99 135
Cuts at: 3030 3145 3229 513
Size: 115 84 3477
Fragments arranged by size:
3477 939 552 391 188 161 135 115
99 84 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2086 3088 3709 4503 4523 2074
Size: 12 1002 621 794 20 3744
Fragments arranged by size:
3744 1002 794 621 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 2638 2967
Size: 54 1425 50 275 18 750 58 329
Cuts at: 2967 3027 3039 3069 3085 3114 3174 3226 3640
Size: 60 12 30 16 29 60 52 414
Cuts at: 3640 4529 6158 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 750 414 329 275 60
60 58 54 52 50 43 30 29
18 16 12
EagI C'GGCC_G
Cuts at: 1537 1812 2580 2638 2967 3027 3174 3226 3640
Size: 275 768 58 329 60 147 52 414
Cuts at: 3640 1537
Size: 4090
Fragments arranged by size:
4090 768 414 329 275 147 60 58
52
EarI CTCTTCn'nnn_
Cuts at: 1353 4123 1353
Size: 2770 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2529 3347 4780 5154 5300 387
Size: 1064 138 940 818 1433 374 146 1280
Fragments arranged by size:
1433 1280 1064 940 818 374 146 138
EcoICRI GAG'CTC
Cuts at: 2344 2344
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2347 2753 3071 3311 3359 3445 3491
Size: 393 715 406 318 240 48 86 46
Cuts at: 3491 4256 4853 5640 5908 1239
Size: 765 597 787 268 1524
Fragments arranged by size:
1524 787 765 715 597 406 393 318
268 240 86 48 46
EcoO109I rG'GnC_Cy
Cuts at: 2065 2477 2478 2521 2522 2764 2813 2891 3012
Size: 412 1 43 1 242 49 78 121
Cuts at: 3012 2065
Size: 5246
Fragments arranged by size:
5246 412 242 121 78 49 43 1
1
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 3259 2209
Size: 1050 5143
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 2125 2147 2389 2616 2629 2954 3113 3382
Size: 447 22 242 227 13 325 159 269
Cuts at: 3382 5340 1678
Size: 1958 2531
Fragments arranged by size:
2531 1958 447 325 269 242 227 159
22 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4834 4866 4908 4940 1373
Size: 32 135 32 3262 32 42 32 2626
Fragments arranged by size:
3262 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KasI G'GCGC_C
Cuts at: 2121 2612 2950 2121
Size: 491 338 5364
Fragments arranged by size:
5364 491 338
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5491 1305
Size: 4002 184 2007
Fragments arranged by size:
4002 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3041 10
Size: 54 1425 343 1209 3162
Fragments arranged by size:
3162 1425 1209 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2310 2605 2675 4151 4510 4669
Size: 648 913 231 295 70 1476 359 159
Cuts at: 4669 518
Size: 2042
Fragments arranged by size:
2042 1476 913 648 359 295 231 159
70
MspA1I CmG'CkG
Cuts at: 1606 2051 2244 2553 2576 2585 2798 2840 2897
Size: 445 193 309 23 9 213 42 57
Cuts at: 2897 3032 3231 3609 4286 5434 5679 1606
Size: 135 199 378 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 445 378 309 245 213
199 193 135 57 42 23 9
NaeI GCC'GGC
Cuts at: 2509 2509
Size: 6193
NarI GG'CG_CC
Cuts at: 2122 2613 2951 2122
Size: 491 338 5364
Fragments arranged by size:
5364 491 338
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NgoMIV G'CCGG_C
Cuts at: 2507 2507
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 2580 2967 3174 3640 2580
Size: 387 207 466 5133
Fragments arranged by size:
5133 466 387 207
NspI r_CATG'y
Cuts at: 1132 1229 2163 3551 5096 1132
Size: 97 934 1388 1545 2229
Fragments arranged by size:
2229 1545 1388 934 97
PasI CC'CwG_GG
Cuts at: 1348 2061 1348
Size: 713 5480
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PmlI CAC'GTG
Cuts at: 3301 3301
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3311 3343 4262 4294 5775 5807 3254
Size: 32 25 32 919 32 1481 32 3640
Fragments arranged by size:
3640 1481 919 32 32 32 32 25
PpuMI rG'GwC_Cy
Cuts at: 2065 3012 2065
Size: 947 5246
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 2477 2521 2764 2813 2890 3064 2477
Size: 44 243 49 77 174 5606
Fragments arranged by size:
5606 243 174 77 49 44
PstI C_TGCA'G
Cuts at: 830 2081 2421 2763 3545 830
Size: 1251 340 342 782 3478
Fragments arranged by size:
3478 1251 782 342 340
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2553 2576 2553
Size: 23 6170
RsrII CG'GwC_CG
Cuts at: 2872 2872
Size: 6193
SacI G_AGCT'C
Cuts at: 2346 2346
Size: 6193
SacII CC_GC'GG
Cuts at: 2586 2799 2898 3033 3232 2586
Size: 213 99 135 199 5547
Fragments arranged by size:
5547 213 199 135 99
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SanDI GG'GwC_CC
Cuts at: 2065 2065
Size: 6193
SbfI CC_TGCA'GG
Cuts at: 2763 3545 2763
Size: 782 5411
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SexAI A'CCwGG_T
Cuts at: 2295 2295
Size: 6193
SfcI C'TryA_G
Cuts at: 826 1045 2077 2417 2759 3541 4676 5357 5548
Size: 219 1032 340 342 782 1135 681 191
Cuts at: 5548 826
Size: 1471
Fragments arranged by size:
1471 1135 1032 782 681 342 340 219
191
SfoI GGC'GCC
Cuts at: 2123 2614 2952 2123
Size: 491 338 5364
Fragments arranged by size:
5364 491 338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 3132 1700
Size: 1432 4761
SmaI CCC'GGG
Cuts at: 1872 2769 3185 3520 1872
Size: 897 416 335 4545
Fragments arranged by size:
4545 897 416 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2381 2443 3374 4297 5198
Size: 197 926 204 234 62 931 923 901
Cuts at: 5198 5460 820
Size: 262 1553
Fragments arranged by size:
1553 931 926 923 901 262 234 204
197 62
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SrfI GCCC'GGGC
Cuts at: 2769 2769
Size: 6193
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2259 3120 3632 513
Size: 552 939 255 861 512 3074
Fragments arranged by size:
3074 939 861 552 512 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4439 6151 96
Size: 274 80 33 51 1443 2462 1712 138
Fragments arranged by size:
2462 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2787 2787
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 2767 3183 3518 1870
Size: 897 416 335 4545
Fragments arranged by size:
4545 897 416 335
XmnI GAAnn'nnTTC
Cuts at: 2684 3242 3269 3326 4322 2684
Size: 558 27 57 996 4555
Fragments arranged by size:
4555 996 558 57 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2014 4001 276
Size: 53 83 186 936 480 1987 2468
Fragments arranged by size:
2468 1987 936 480 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI
PfoI PspXI PsrI SapI SfiI SwaI