Restriction map for
pFN21AE4115
(Circular) (Six-base) MAPSORT of: pFN21AE4115.seq Check: 4255 from: 1 to: 6169
pFN21AE4115 6169 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 4232 5616 5884 1632
Size: 845 1755 1384 268 1917
Fragments arranged by size:
1917 1755 1384 845 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 2391 5068 6045 1203
Size: 1188 2677 977 1327
Fragments arranged by size:
2677 1327 1188 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 3453 4898 3453
Size: 1445 4724
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 2106 2692 3912 5484 2106
Size: 586 1220 1572 2791
Fragments arranged by size:
2791 1572 1220 586
ApaI G_GGCC'C
Cuts at: 2267 2267
Size: 6169
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 3478 3704 3856 5965 1493
Size: 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 3494 1870
Size: 73 1551 4545
Fragments arranged by size:
4545 1551 73
BamHI G'GATC_C
Cuts at: 2550 3105 3499 3866 2550
Size: 555 394 367 4853
Fragments arranged by size:
4853 555 394 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3490 4945 618
Size: 325 217 627 1703 1455 1842
Fragments arranged by size:
1842 1703 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2067 2267 2703 2845 3044 3488 1391
Size: 676 200 436 142 199 444 4072
Fragments arranged by size:
4072 676 444 436 200 199 142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3101 928
Size: 971 1202 3996
Fragments arranged by size:
3996 1202 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3197 4061 5271 3197
Size: 864 1210 4095
Fragments arranged by size:
4095 1210 864
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2218 4780 136
Size: 107 122 71 631 605 546 2562 1525
Fragments arranged by size:
2562 1525 631 605 546 122 107 71
BglII A'GATC_T
Cuts at: 2848 6164 2848
Size: 3316 2853
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2267 3291 4230 5386 1790
Size: 477 1024 939 1156 2573
Fragments arranged by size:
2573 1156 1024 939 477
BmgBI CAC'GTC
Cuts at: 1318 2911 1318
Size: 1593 4576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2680 2911 3082
Size: 416 53 83 399 24 1263 231 171
Cuts at: 3082 3140 3217 3900 4858 442
Size: 58 77 683 958 1753
Fragments arranged by size:
1753 1263 958 683 416 399 231 171
83 77 58 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2735 3166 4829 1239
Size: 842 654 431 1663 2579
Fragments arranged by size:
2579 1663 842 654 431
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 6169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4294 5159 5457 4294
Size: 865 298 5006
Fragments arranged by size:
5006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2373 4832 882
Size: 617 874 2459 2219
Fragments arranged by size:
2459 2219 874 617
BsaAI yAC'GTr
Cuts at: 493 1204 3960 493
Size: 711 2756 2702
Fragments arranged by size:
2756 2702 711
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3976 4358 4993 275
Size: 53 83 186 936 2443 382 635 1451
Fragments arranged by size:
2443 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2961 4602 5000 5274 5421 5832 2961
Size: 1641 398 274 147 411 3298
Fragments arranged by size:
3298 1641 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2976 3006 1178
Size: 30 165 30 1573 30 4341
Fragments arranged by size:
4341 1573 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2480 3046 1186
Size: 1234 60 566 4309
Fragments arranged by size:
4309 1234 566 60
BseYI C'CCAG_C
Cuts at: 3045 5372 5868 3045
Size: 2327 496 3346
Fragments arranged by size:
3346 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 1163
Size: 573 580 5016
Fragments arranged by size:
5016 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2379 2703 4230 4315 5386 2067
Size: 312 324 1527 85 1071 2850
Fragments arranged by size:
2850 1527 1071 324 312 85
BsmI GAATG_Cn'
Cuts at: 3680 3773 3680
Size: 93 6076
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2267 2379 2703 2845 3044 3291
Size: 399 277 200 112 324 142 199 247
Cuts at: 3291 3488 4230 4315 5386 1391
Size: 197 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 277 247 200
199 197 142 112 85
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 2214 3524 844
Size: 1210 160 1310 3489
Fragments arranged by size:
3489 1310 1210 160
BsrBI CCG'CTC
Cuts at: 1394 1823 2365 4056 1394
Size: 429 542 1691 3507
Fragments arranged by size:
3507 1691 542 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4813 5000 1700
Size: 110 3003 187 2869
Fragments arranged by size:
3003 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 2399 4229 5241 2399
Size: 1830 1012 3327
Fragments arranged by size:
3327 1830 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6169
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 2041 2384 1184
Size: 857 343 4969
Fragments arranged by size:
4969 857 343
BstYI r'GATC_y
Cuts at: 1972 2550 2710 2848 3105 3499 3866 4251 4268
Size: 578 160 138 257 394 367 385 17
Cuts at: 4268 5720 5806 5818 6164 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 578 394 385 367 346 257
160 138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2795 2795
Size: 6169
BtgI C'CryG_G
Cuts at: 513 1065 2302 2380 3358 513
Size: 552 1237 78 978 3324
Fragments arranged by size:
3324 1237 978 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 3685 4479 4499 3685
Size: 794 20 5355
Fragments arranged by size:
5355 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 2940 3473 2940
Size: 533 5636
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3006 3032 5176 809
Size: 507 1690 26 2144 1802
Fragments arranged by size:
2144 1802 1690 507 26
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2226 3087
Size: 54 1425 50 275 18 265 131 861
Cuts at: 3087 3616 4505 6134 8
Size: 529 889 1629 43
Fragments arranged by size:
1629 1425 889 861 529 275 265 131
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2327 2622 2649 4099 1353
Size: 678 296 295 27 1450 3423
Fragments arranged by size:
3423 1450 678 296 295 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4756 5130 5276 387
Size: 1064 138 3167 374 146 1280
Fragments arranged by size:
3167 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2065 2701 2065
Size: 636 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2477 2735 3166 4232 4829 5616
Size: 393 449 396 258 431 1066 597 787
Cuts at: 5616 5884 1239
Size: 268 1524
Fragments arranged by size:
1524 1066 787 597 449 431 396 393
268 258
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2402
Size: 6169
EcoO109I rG'GnC_Cy
Cuts at: 2264 3134 3243 2264
Size: 870 109 5190
Fragments arranged by size:
5190 870 109
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2306 2306
Size: 6169
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 5316 1678
Size: 3638 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2535 2567 2688 2720 2905
Size: 32 135 32 963 32 121 32 185
Cuts at: 2905 2937 3439 3471 4810 4842 4884 4916 1373
Size: 32 502 32 1339 32 42 32 2626
Fragments arranged by size:
2626 1339 963 502 185 135 121 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2908 3513 3759 669
Size: 988 281 798 172 605 246 3079
Fragments arranged by size:
3079 988 798 605 281 246 172
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5283 5467 1305
Size: 3978 184 2007
Fragments arranged by size:
3978 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2097 2228 3089 10
Size: 54 1425 343 265 131 861 3090
Fragments arranged by size:
3090 1425 861 343 265 131 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3231 3284 4127 4486 4645 518
Size: 648 2065 53 843 359 159 2042
Fragments arranged by size:
2065 2042 843 648 359 159 53
MspA1I CmG'CkG
Cuts at: 1606 2278 2572 3585 4262 5410 5655 1606
Size: 672 294 1013 677 1148 245 2120
Fragments arranged by size:
2120 1148 1013 677 672 294 245
NcoI C'CATG_G
Cuts at: 513 1065 2302 3358 513
Size: 552 1237 1056 3324
Fragments arranged by size:
3324 1237 1056 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3527 5072 1132
Size: 97 2298 1545 2229
Fragments arranged by size:
2298 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2267 1348
Size: 919 5250
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PfoI T'CCnGG_A
Cuts at: 2516 2516
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3134 3134
Size: 6169
PshAI GACnn'nnGTC
Cuts at: 1613 2888 1613
Size: 1275 4894
PsiI TTA'TAA
Cuts at: 3345 3739 3345
Size: 394 5775
PspOMI G'GGCC_C
Cuts at: 2263 2263
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2319 2754 3521 830
Size: 1489 435 767 3478
Fragments arranged by size:
3478 1489 767 435
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 2278 2572 2278
Size: 294 5875
SacI G_AGCT'C
Cuts at: 2067 2703 2067
Size: 636 5533
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SapI GCTCTTCn'nnn_
Cuts at: 2327 2327
Size: 6169
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 2019 4417 6129 2019
Size: 2398 1712 2059
Fragments arranged by size:
2398 2059 1712
SfcI C'TryA_G
Cuts at: 826 1045 2315 2750 3028 3517 4652 5333 5524
Size: 219 1270 435 278 489 1135 681 191
Cuts at: 5524 826
Size: 1471
Fragments arranged by size:
1471 1270 1135 681 489 435 278 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 3496 1872
Size: 1624 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4273 5174 5436 820
Size: 197 926 2330 901 262 1553
Fragments arranged by size:
2330 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 3389 4093 5
Size: 47 3337 704 2081
Fragments arranged by size:
3337 2081 704 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6169
StyI C'CwwG_G
Cuts at: 513 1065 2302 3358 3608 513
Size: 552 1237 1056 250 3074
Fragments arranged by size:
3074 1237 1056 552 250
SwaI ATTT'AAAT
Cuts at: 2940 2940
Size: 6169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3199 4196 4213 4366 4551 4996 325
Size: 1255 1619 997 17 153 185 445 1498
Fragments arranged by size:
1619 1498 1255 997 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2017 4415 6127
Size: 274 80 33 51 1443 40 2398 1712
Cuts at: 6127 96
Size: 138
Fragments arranged by size:
2398 1712 1443 274 138 80 51 40
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3195 3227 6129 6161 3195
Size: 32 2902 32 3203
Fragments arranged by size:
3203 2902 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2686 1316
Size: 1370 4799
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 3494 1870
Size: 1624 4545
XmnI GAAnn'nnTTC
Cuts at: 4298 4298
Size: 6169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI XcmI