Restriction map for
pFN21AE4113
(Circular) (Six-base) MAPSORT of: pFN21AE4113.seq Check: 686 from: 1 to: 6160
pFN21AE4113 6160 bp
With 182 enzymes: SgfI *
March 28, 2012 13:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 3060 3481 3060
Size: 421 5739
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4223 5607 5875 1632
Size: 2591 1384 268 1917
Fragments arranged by size:
2591 1917 1384 268
AfeI AGC'GCT
Cuts at: 2621 2621
Size: 6160
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 2433 5059 6036 1203
Size: 1230 2626 977 1327
Fragments arranged by size:
2626 1327 1230 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3252 3284 3252
Size: 32 6128
AlwNI CAG_nnn'CTG
Cuts at: 2698 2797 3903 5475 2698
Size: 99 1106 1572 3383
Fragments arranged by size:
3383 1572 1106 99
ApaI G_GGCC'C
Cuts at: 2277 2277
Size: 6160
ApaLI G'TGCA_C
Cuts at: 2258 4217 5373 2258
Size: 1959 1156 3045
Fragments arranged by size:
3045 1959 1156
ApoI r'AATT_y
Cuts at: 1493 2382 2856 3469 3695 3847 5956 1493
Size: 889 474 613 226 152 2109 1697
Fragments arranged by size:
2109 1697 889 613 474 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2278 2606 2715 3185 3485 1870
Size: 73 335 328 109 470 300 4545
Fragments arranged by size:
4545 470 335 328 300 109 73
BamHI G'GATC_C
Cuts at: 3230 3490 3857 3230
Size: 260 367 5533
Fragments arranged by size:
5533 367 260
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2296 3015 3060 3481
Size: 325 217 627 282 227 719 45 421
Cuts at: 3481 4936 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 719 627 421 325 282 227
217 45
BanII G_rGCy'C
Cuts at: 1391 2183 2247 2277 2406 2607 2842 3177 3447
Size: 792 64 30 129 201 235 335 270
Cuts at: 3447 3479 1391
Size: 32 4072
Fragments arranged by size:
4072 792 335 270 235 201 129 64
32 30
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 6160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2329 2740 3024 928
Size: 971 430 411 284 4064
Fragments arranged by size:
4064 971 430 411 284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2654 3132
Size: 107 122 71 631 605 337 645 478
Cuts at: 3132 4771 136
Size: 1639 1525
Fragments arranged by size:
1639 1525 645 631 605 478 337 122
107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2277 2301 4221 5377 1790
Size: 472 15 24 1920 1156 2573
Fragments arranged by size:
2573 1920 1156 472 24 15
BmgBI CAC'GTC
Cuts at: 1318 2257 2398 1318
Size: 939 141 5080
Fragments arranged by size:
5080 939 141
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2776 2893 3426
Size: 416 53 83 399 24 1359 117 533
Cuts at: 3426 3891 4849 442
Size: 465 958 1753
Fragments arranged by size:
1753 1359 958 533 465 416 399 117
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2561 2720 2939 3121 4820 1239
Size: 1322 159 219 182 1699 2579
Fragments arranged by size:
2579 1699 1322 219 182 159
Bpu10I CC'TnA_GC
Cuts at: 3441 3441
Size: 6160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3161 4285 5150 5448 3161
Size: 1124 865 298 3873
Fragments arranged by size:
3873 1124 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2352 2918 4823 882
Size: 617 853 566 1905 2219
Fragments arranged by size:
2219 1905 853 617 566
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 3967 4349 4984
Size: 53 83 186 936 537 1897 382 635
Cuts at: 4984 275
Size: 1451
Fragments arranged by size:
1897 1451 936 635 537 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5935
Fragments arranged by size:
5935 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 2891 2936 3385 1186
Size: 1429 276 45 449 3961
Fragments arranged by size:
3961 1429 449 276 45
BseYI C'CCAG_C
Cuts at: 2108 2509 2625 3121 5363 5859 2108
Size: 401 116 496 2242 496 2409
Fragments arranged by size:
2409 2242 496 496 401 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3380 1163
Size: 573 1644 3943
Fragments arranged by size:
3943 1644 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 3610 4371 5399 1540
Size: 275 186 42 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2247 2262 3177 3395 3447 4221 4306 5377 2247
Size: 15 915 218 52 774 85 1071 3030
Fragments arranged by size:
3030 1071 915 774 218 85 52 15
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2247 2262 2277 2301 2406 2607
Size: 399 393 64 15 15 24 105 201
Cuts at: 2607 2842 3177 3395 3447 3479 4221 4306 5377
Size: 235 335 218 52 32 742 85 1071
Cuts at: 5377 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 393 335 235 218
201 105 85 64 52 32 24 15
15
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3515 844
Size: 2671 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 2589 2688 4804 4991 1700
Size: 110 256 523 99 2116 187 2869
Fragments arranged by size:
2869 2116 523 256 187 110 99
BsrGI T'GTAC_A
Cuts at: 96 2436 96
Size: 2340 3820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2797 794
Size: 2003 4157
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 3230 3435 3490 3857 4242 4259 5711 5797
Size: 1258 205 55 367 385 17 1452 86
Cuts at: 5797 5809 6155 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1258 385 367 346 205 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2043 2076 3064 513
Size: 552 939 33 6 33 988 3609
Fragments arranged by size:
3609 988 939 552 33 33 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 3099 3368 3676 4470 4490 3099
Size: 269 308 794 20 4769
Fragments arranged by size:
4769 794 308 269 20
ClaI AT'CG_AT
Cuts at: 1554 2451 1554
Size: 897 5263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2250 2475
Size: 54 1425 50 275 18 210 210 225
Cuts at: 2475 2952 3297 3607 4496 6125 8
Size: 477 345 310 889 1629 43
Fragments arranged by size:
1629 1425 889 477 345 310 275 225
210 210 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 3607 1537
Size: 275 228 1567 4090
Fragments arranged by size:
4090 1567 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 4090 1353
Size: 2737 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 2381 2792 4747 5121 5267
Size: 1064 138 438 354 411 1955 374 146
Cuts at: 5267 387
Size: 1280
Fragments arranged by size:
1955 1280 1064 438 411 374 354 146
138
EcoICRI GAG'CTC
Cuts at: 2245 3175 3445 2245
Size: 930 270 4960
Fragments arranged by size:
4960 930 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2561 2720 2939 3121 4223 4820 5607
Size: 393 929 159 219 182 1102 597 787
Cuts at: 5607 5875 1239
Size: 268 1524
Fragments arranged by size:
1524 1102 929 787 597 393 268 219
182 159
EcoO109I rG'GnC_Cy
Cuts at: 2273 2274 2722 2998 2273
Size: 1 448 276 5435
Fragments arranged by size:
5435 448 276 1
EcoRI G'AATT_C
Cuts at: 2856 3469 3847 2856
Size: 613 378 5169
Fragments arranged by size:
5169 613 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 3394 2332
Size: 1062 5098
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2037 2073 2115 2129 2623 2895 3073 5307
Size: 359 36 42 14 494 272 178 2234
Cuts at: 5307 1678
Size: 2531
Fragments arranged by size:
2531 2234 494 359 272 178 42 36
14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373
Size: 32 135 32 3229 32 42 32 2626
Fragments arranged by size:
3229 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 3504 3750 669
Size: 988 281 595 971 246 3079
Fragments arranged by size:
3079 988 971 595 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 6160
KpnI G_GTAC'C
Cuts at: 3064 3485 3064
Size: 421 5739
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2885 5274 5458 1305
Size: 1580 2389 184 2007
Fragments arranged by size:
2389 2007 1580 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 10
Size: 54 1425 343 645 3693
Fragments arranged by size:
3693 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4118 4477 4636 518
Size: 648 2952 359 159 2042
Fragments arranged by size:
2952 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2045 3066 3576 4253 5401 5646 1606
Size: 433 6 1021 510 677 1148 245 2120
Fragments arranged by size:
2120 1148 1021 677 510 433 245 6
NaeI GCC'GGC
Cuts at: 2068 2591 2068
Size: 523 5637
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 6160
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4669
Fragments arranged by size:
4669 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NgoMIV G'CCGG_C
Cuts at: 2066 2589 2066
Size: 523 5637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 2040 3607 2040
Size: 1567 4593
NspI r_CATG'y
Cuts at: 1132 1229 2437 2828 3518 5063 1132
Size: 97 1208 391 690 1545 2229
Fragments arranged by size:
2229 1545 1208 690 391 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6160
PciI A'CATG_T
Cuts at: 2433 5059 2433
Size: 2626 3534
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PfoI T'CCnGG_A
Cuts at: 3309 3309
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3419 1613
Size: 1806 4354
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspOMI G'GGCC_C
Cuts at: 2273 2273
Size: 6160
PstI C_TGCA'G
Cuts at: 830 3512 830
Size: 2682 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 6160
SacI G_AGCT'C
Cuts at: 2247 3177 3447 2247
Size: 930 270 4960
Fragments arranged by size:
4960 930 270
SacII CC_GC'GG
Cuts at: 2040 2046 3067 2040
Size: 6 1021 5133
Fragments arranged by size:
5133 1021 6
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 3508 4643 5324 5515 826
Size: 219 2463 1135 681 191 1471
Fragments arranged by size:
2463 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2654 2654
Size: 6160
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 6160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 2066 1700
Size: 366 5794
SmaI CCC'GGG
Cuts at: 1872 2280 3487 1872
Size: 408 1207 4545
Fragments arranged by size:
4545 1207 408
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3176 4264 5165 5427 820
Size: 197 926 1233 1088 901 262 1553
Fragments arranged by size:
1553 1233 1088 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 2004 3599 513
Size: 552 939 1595 3074
Fragments arranged by size:
3074 1595 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4187 4204 4357 4542 4987 325
Size: 1255 2607 17 153 185 445 1498
Fragments arranged by size:
2607 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 2436 2499
Size: 274 80 33 51 1443 453 6 63
Cuts at: 2499 3156 4406 6118 96
Size: 657 1250 1712 138
Fragments arranged by size:
1712 1443 1250 657 453 274 138 80
63 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 6120 6152 3347
Size: 32 2741 32 3355
Fragments arranged by size:
3355 2741 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2740 3307 2740
Size: 567 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 2278 3485 1870
Size: 408 1207 4545
Fragments arranged by size:
4545 1207 408
XmnI GAAnn'nnTTC
Cuts at: 4289 4289
Size: 6160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI RsrII SacI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
PvuII SanDI SapI SexAI SrfI SwaI