Restriction map for
pFN21AE4112
(Circular) (Six-base) MAPSORT of: pFN21AE4112.seq Check: 5633 from: 1 to: 6154
pFN21AE4112 6154 bp
With 182 enzymes: SgfI *
March 28, 2012 13:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2105 2179 2568 2875 3273 3497 1937
Size: 168 74 389 307 398 224 4594
Fragments arranged by size:
4594 398 389 307 224 168 74
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4217 5601 5869 1632
Size: 2585 1384 268 1917
Fragments arranged by size:
2585 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3119 820
Size: 197 2102 3855
Fragments arranged by size:
3855 2102 197
AflIII A'CryG_T
Cuts at: 1203 5053 6030 1203
Size: 3850 977 1327
Fragments arranged by size:
3850 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AlwNI CAG_nnn'CTG
Cuts at: 3320 3897 5469 3320
Size: 577 1572 4005
Fragments arranged by size:
4005 1572 577
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 2081 2885 3463 3689 3841 5950 1493
Size: 588 804 578 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 588 578 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 3479 1870
Size: 73 1536 4545
Fragments arranged by size:
4545 1536 73
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3475 4930 618
Size: 325 217 627 1688 1455 1842
Fragments arranged by size:
1842 1688 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2509 3473 1391
Size: 1118 964 4072
Fragments arranged by size:
4072 1118 964
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3416 3450 4345 4379 1903
Size: 34 50 34 1395 34 895 34 3678
Fragments arranged by size:
3678 1395 895 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BfrBI ATG'CAT
Cuts at: 2848 3358 2848
Size: 510 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4765 136
Size: 107 122 71 631 605 3093 1525
Fragments arranged by size:
3093 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2830 6149 2830
Size: 3319 2835
BlpI GC'TnA_GC
Cuts at: 3571 3571
Size: 6154
Bme1580I G_kGCm'C
Cuts at: 1790 2842 2932 4215 5371 1790
Size: 1052 90 1283 1156 2573
Fragments arranged by size:
2573 1283 1156 1052 90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3885 4843 442
Size: 416 53 83 399 24 2468 958 1753
Fragments arranged by size:
2468 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3204 4814 1239
Size: 1965 1610 2579
Fragments arranged by size:
2579 1965 1610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4279 5144 5442 4279
Size: 865 298 4991
Fragments arranged by size:
4991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4817 882
Size: 617 3318 2219
Fragments arranged by size:
3318 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3945 493
Size: 711 2741 2702
Fragments arranged by size:
2741 2702 711
BsaBI GATnn'nnATC
Cuts at: 6148 6148
Size: 6154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3961 4343 4978 275
Size: 53 83 186 936 2428 382 635 1451
Fragments arranged by size:
2428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4587 4985 5259 5406 5817 4587
Size: 398 274 147 411 4924
Fragments arranged by size:
4924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 1178
Size: 30 165 30 1059 30 4840
Fragments arranged by size:
4840 1059 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2169 1186
Size: 983 5171
BseYI C'CCAG_C
Cuts at: 2408 2465 5357 5853 2408
Size: 57 2892 496 2709
Fragments arranged by size:
2892 2709 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2571 1163
Size: 573 835 4746
Fragments arranged by size:
4746 835 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2509 2872 4215 4300 5371 2509
Size: 363 1343 85 1071 3292
Fragments arranged by size:
3292 1343 1071 363 85
BsmI GAATG_Cn'
Cuts at: 3665 3758 3665
Size: 93 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2509 2842 2872 2932 3473 4215 4300
Size: 399 719 333 30 60 541 742 85
Cuts at: 4300 5371 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 719 541 399 333 85
60 30
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 2254 4043 5773 2254
Size: 1789 1730 2635
Fragments arranged by size:
2635 1789 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3509 844
Size: 2665 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4041 1394
Size: 429 2218 3507
Fragments arranged by size:
3507 2218 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4649 4823 65
Size: 1183 3401 174 1396
Fragments arranged by size:
3401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4798 4985 1700
Size: 110 2988 187 2869
Fragments arranged by size:
2988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 2241 4214 5226 2241
Size: 1973 1012 3169
Fragments arranged by size:
3169 1973 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3320 794
Size: 2526 3628
BstBI TT'CG_AA
Cuts at: 3232 4958 3232
Size: 1726 4428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstYI r'GATC_y
Cuts at: 1972 2621 2830 3484 3851 4236 4253 5705 5791
Size: 649 209 654 367 385 17 1452 86
Cuts at: 5791 5803 6149 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 654 649 385 367 346 209
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 3670 4464 4484 2410
Size: 1260 794 20 4080
Fragments arranged by size:
4080 1260 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2626 3090 3601
Size: 54 1425 50 275 18 796 464 511
Cuts at: 3601 4490 6119 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 796 511 464 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4084 1353
Size: 2731 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4741 5115 5261 387
Size: 1064 138 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2507 2507
Size: 6154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3204 4217 4814 5601 5869 1239
Size: 393 1572 1013 597 787 268 1524
Fragments arranged by size:
1572 1524 1013 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3463 3841 3463
Size: 378 5776
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 5301 1678
Size: 3623 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2741 2773 2896 2928 4795
Size: 32 135 32 1169 32 123 32 1867
Cuts at: 4795 4827 4869 4901 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1867 1169 135 123 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 3498 3744 669
Size: 988 281 223 1337 246 3079
Fragments arranged by size:
3079 1337 988 281 246 223
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2330 3386 5268 5452 1305
Size: 1025 1056 1882 184 2007
Fragments arranged by size:
2007 1882 1056 1025 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3092 10
Size: 54 1425 343 1260 3072
Fragments arranged by size:
3072 1425 1260 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 4112 4471 4630 518
Size: 648 1005 1941 359 159 2042
Fragments arranged by size:
2042 1941 1005 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2465 3320 3346 3570 4247 5395 5640 1606
Size: 859 855 26 224 677 1148 245 2120
Fragments arranged by size:
2120 1148 859 855 677 245 224 26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5602
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NsiI A_TGCA'T
Cuts at: 2850 3360 2850
Size: 510 5644
NspI r_CATG'y
Cuts at: 1132 1229 2784 3272 3512 5057 1132
Size: 97 1555 488 240 1545 2229
Fragments arranged by size:
2229 1555 1545 488 240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6154
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2286 4223 4255 5736 5768 2254
Size: 32 1937 32 1481 32 2640
Fragments arranged by size:
2640 1937 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2224 1613
Size: 611 5543
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PstI C_TGCA'G
Cuts at: 830 3325 3506 830
Size: 2495 181 3478
Fragments arranged by size:
3478 2495 181
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
PvuII CAG'CTG
Cuts at: 2465 3320 3346 2465
Size: 855 26 5273
Fragments arranged by size:
5273 855 26
SacI G_AGCT'C
Cuts at: 2509 2509
Size: 6154
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 2131 4402 6114 2131
Size: 2271 1712 2171
Fragments arranged by size:
2271 2171 1712
SfcI C'TryA_G
Cuts at: 826 1045 3321 3502 4637 5318 5509 826
Size: 219 2276 181 1135 681 191 1471
Fragments arranged by size:
2276 1471 1135 681 219 191 181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 3481 1872
Size: 1609 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3119 4258 5159 5421 820
Size: 197 926 1176 1139 901 262 1553
Fragments arranged by size:
1553 1176 1139 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 3026 4078 5
Size: 47 2974 1052 2081
Fragments arranged by size:
2974 2081 1052 47
StuI AGG'CCT
Cuts at: 1517 3181 1517
Size: 1664 4490
StyI C'CwwG_G
Cuts at: 513 1065 2010 2109 3593 513
Size: 552 945 99 1484 3074
Fragments arranged by size:
3074 1484 945 552 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4181 4198 4351 4536 4981 325
Size: 1255 2601 17 153 185 445 1498
Fragments arranged by size:
2601 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2129 4400 6112
Size: 274 80 33 51 1443 152 2271 1712
Cuts at: 6112 96
Size: 138
Fragments arranged by size:
2271 1712 1443 274 152 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6114 6146 6114
Size: 32 6122
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3407 3407
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3479 1870
Size: 1609 4545
XmnI GAAnn'nnTTC
Cuts at: 2131 2689 4283 2131
Size: 558 1594 4002
Fragments arranged by size:
4002 1594 558
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI