Restriction map for
pFN21AE4110
(Circular) (Six-base) MAPSORT of: pFN21AE4110.seq Check: 3951 from: 1 to: 6148
pFN21AE4110 6148 bp
With 182 enzymes: SgfI *
March 28, 2012 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3283 3283
Size: 6148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 2066 3469 2066
Size: 1403 4745
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3296 4211 5595 5863 1632
Size: 1664 915 1384 268 1917
Fragments arranged by size:
1917 1664 1384 915 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 3099 5047 6024 1203
Size: 1896 1948 977 1327
Fragments arranged by size:
1948 1896 1327 977
AgeI A'CCGG_T
Cuts at: 2809 4979 2809
Size: 2170 3978
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 3104 1166
Size: 1938 4210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 2663 3457 3683 3835 5944 1493
Size: 1170 794 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 794 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3351 3473 1870
Size: 73 1408 122 4545
Fragments arranged by size:
4545 1408 122 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 3469 4924 618
Size: 325 217 627 279 1403 1455 1842
Fragments arranged by size:
1842 1455 1403 627 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2754 3001 3254 3360 3414 3446 3467 1391
Size: 1363 247 253 106 54 32 21 4072
Fragments arranged by size:
4072 1363 253 247 106 54 32 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2221 4759 136
Size: 107 122 71 631 605 549 2538 1525
Fragments arranged by size:
2538 1525 631 605 549 122 107 71
BglII A'GATC_T
Cuts at: 2319 2688 6143 2319
Size: 369 3455 2324
Fragments arranged by size:
3455 2324 369
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 4209 5365 1790
Size: 2419 1156 2573
Fragments arranged by size:
2573 2419 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2285 3879 4837
Size: 416 53 83 399 24 868 1594 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
1753 1594 958 868 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3321 3321
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2243 2429 2741 4808 1239
Size: 938 66 186 312 2067 2579
Fragments arranged by size:
2579 2067 938 312 186 66
Bpu10I CC'TnA_GC
Cuts at: 3440 3440
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2668 4273 5138 5436 2668
Size: 1605 865 298 3380
Fragments arranged by size:
3380 1605 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4811 882
Size: 617 3312 2219
Fragments arranged by size:
3312 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 3939 493
Size: 711 1898 837 2702
Fragments arranged by size:
2702 1898 837 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2809 4581 4979 5253 5400 5811 2809
Size: 1772 398 274 147 411 3146
Fragments arranged by size:
3146 1772 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3194 3224 1178
Size: 30 165 30 1791 30 4102
Fragments arranged by size:
4102 1791 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2303 2492 2738 2963 3215 1186
Size: 988 129 189 246 225 252 4119
Fragments arranged by size:
4119 988 252 246 225 189 129
BseYI C'CCAG_C
Cuts at: 2375 2681 2830 2856 3039 3324 5351 5847 2375
Size: 306 149 26 183 285 2027 496 2676
Fragments arranged by size:
2676 2027 496 306 285 183 149 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3124 3188 1163
Size: 573 1388 64 4123
Fragments arranged by size:
4123 1388 573 64
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2310 2385 2707 2754 3001 3446 4209 4294 5365
Size: 75 322 47 247 445 763 85 1071
Cuts at: 5365 2310
Size: 3093
Fragments arranged by size:
3093 1071 763 445 322 247 85 75
47
BsmI GAATG_Cn'
Cuts at: 3659 3752 3659
Size: 93 6055
BsmBI CGTCTCn'nnnn_
Cuts at: 3244 3244
Size: 6148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2385 2707 2754 3001 3254 3360
Size: 399 520 75 322 47 247 253 106
Cuts at: 3360 3414 3446 3467 4209 4294 5365 1391
Size: 54 32 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 322 253 247
106 85 75 54 47 32 21
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3283 3503 844
Size: 2439 220 3489
Fragments arranged by size:
3489 2439 220
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 2694 2809 4792 4979 1700
Size: 110 270 614 115 1983 187 2869
Fragments arranged by size:
2869 1983 614 270 187 115 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 3328 1184
Size: 2144 4004
BstYI r'GATC_y
Cuts at: 1972 2319 2688 3478 3845 4230 4247 5699 5785
Size: 347 369 790 367 385 17 1452 86
Cuts at: 5785 5797 6143 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 790 385 369 367 347 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2072 2579 2072
Size: 507 5641
BtgI C'CryG_G
Cuts at: 513 1065 2004 3127 3144 513
Size: 552 939 1123 17 3517
Fragments arranged by size:
3517 1123 939 552 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3372 3664 4458 4478 3372
Size: 292 794 20 5042
Fragments arranged by size:
5042 794 292 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2536 5155 809
Size: 507 1220 2619 1802
Fragments arranged by size:
2619 1802 1220 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3194 3595
Size: 54 1425 50 275 18 268 1096 401
Cuts at: 3595 4484 6113 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1096 889 401 275 268 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4078 1353
Size: 2725 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2752 2999 3444 2752
Size: 247 445 5456
Fragments arranged by size:
5456 445 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2243 2429 2741 3296 4211 4808
Size: 393 545 66 186 312 555 915 597
Cuts at: 4808 5595 5863 1239
Size: 787 268 1524
Fragments arranged by size:
1524 915 787 597 555 545 393 312
268 186 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 3385 2531
Size: 854 5294
EcoO109I rG'GnC_Cy
Cuts at: 2863 2863
Size: 6148
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2341 2992 2341
Size: 651 5497
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 2257 5295 1678
Size: 579 3038 2531
Fragments arranged by size:
3038 2531 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 3345 3377 4789
Size: 32 135 32 732 32 1009 32 1412
Cuts at: 4789 4821 4863 4895 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1412 1009 732 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2047 3492 3738 669
Size: 988 281 109 1445 246 3079
Fragments arranged by size:
3079 1445 988 281 246 109
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 2070 3473 2070
Size: 1403 4745
MfeI C'AATT_G
Cuts at: 2621 3747 2621
Size: 1126 5022
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2898 5262 5446 1305
Size: 1593 2364 184 2007
Fragments arranged by size:
2364 2007 1593 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3104 4106 4465 4624 518
Size: 648 1938 1002 359 159 2042
Fragments arranged by size:
2042 1938 1002 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2263 2420 2872 3138 3172 3564 4241
Size: 621 36 157 452 266 34 392 677
Cuts at: 4241 5389 5634 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 621 452 392 266 245
157 36 34
NcoI C'CATG_G
Cuts at: 513 1065 2004 3127 513
Size: 552 939 1123 3534
Fragments arranged by size:
3534 1123 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 3506 5051 1132
Size: 97 2277 1545 2229
Fragments arranged by size:
2277 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PfoI T'CCnGG_A
Cuts at: 2406 2406
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PmlI CAC'GTG
Cuts at: 3102 3102
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2877 3500 830
Size: 2047 623 3478
Fragments arranged by size:
3478 2047 623
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2227 2263 3138 2227
Size: 36 875 5237
Fragments arranged by size:
5237 875 36
SacI G_AGCT'C
Cuts at: 2754 3001 3446 2754
Size: 247 445 5456
Fragments arranged by size:
5456 445 247
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2428 2873 3496 4631 5312 5503 826
Size: 219 1383 445 623 1135 681 191 1471
Fragments arranged by size:
1471 1383 1135 681 623 445 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2647 4252 5153 5415 820
Size: 197 926 704 1605 901 262 1553
Fragments arranged by size:
1605 1553 926 901 704 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2004 3127 3587 513
Size: 552 939 1123 460 3074
Fragments arranged by size:
3074 1123 939 552 460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3094 3316 4175 4192 4345 4530 4975
Size: 1255 1514 222 859 17 153 185 445
Cuts at: 4975 325
Size: 1498
Fragments arranged by size:
1514 1498 1255 859 445 222 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4394 6106 96
Size: 274 80 33 51 1443 2417 1712 138
Fragments arranged by size:
2417 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3194 3226 6108 6140 3194
Size: 32 2882 32 3202
Fragments arranged by size:
3202 2882 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 2590 2944 3484 2590
Size: 354 540 5254
Fragments arranged by size:
5254 540 354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 2278 2404 3064 2224
Size: 54 126 660 5308
Fragments arranged by size:
5308 660 126 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 4277 4277
Size: 6148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI