Restriction map for
pFN21AE4109
(Circular) (Six-base) MAPSORT of: pFN21AE4109.seq Check: 9608 from: 1 to: 6142
pFN21AE4109 6142 bp
With 182 enzymes: SgfI *
March 28, 2012 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2225 3426 2225
Size: 1201 4941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3269 4205 5589 5857 1632
Size: 1637 936 1384 268 1917
Fragments arranged by size:
1917 1637 1384 936 268
AfeI AGC'GCT
Cuts at: 2609 2609
Size: 6142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 2420 1166
Size: 1254 4888
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2508 1184
Size: 1324 4818
AlwNI CAG_nnn'CTG
Cuts at: 2803 3349 3418 3885 5457 2803
Size: 546 69 467 1572 3488
Fragments arranged by size:
3488 1572 546 467 69
ApaI G_GGCC'C
Cuts at: 2222 2222
Size: 6142
ApaLI G'TGCA_C
Cuts at: 2224 2951 4199 5355 2224
Size: 727 1248 1156 3011
Fragments arranged by size:
3011 1248 1156 727
ApoI r'AATT_y
Cuts at: 1493 3451 3677 3829 5938 1493
Size: 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2873 2873
Size: 6142
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2199 2633 2986 3377 3389 3467
Size: 73 71 185 434 353 391 12 78
Cuts at: 3467 1870
Size: 4545
Fragments arranged by size:
4545 434 391 353 185 78 73 71
12
BamHI G'GATC_C
Cuts at: 3472 3839 3472
Size: 367 5775
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2257 2265 3069 3463 4918
Size: 325 217 627 470 8 804 394 1455
Cuts at: 4918 618
Size: 1842
Fragments arranged by size:
1842 1455 804 627 470 394 325 217
8
BanII G_rGCy'C
Cuts at: 1391 2015 2222 2319 2442 3024 3135 3396 3461
Size: 624 207 97 123 582 111 261 65
Cuts at: 3461 1391
Size: 4072
Fragments arranged by size:
4072 624 582 261 207 123 111 97
65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5171
BbvCI CC'TCA_GC
Cuts at: 2248 3173 2248
Size: 925 5217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3207 3241 4333 4367 1903
Size: 34 1270 34 1092 34 3678
Fragments arranged by size:
3678 1270 1092 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2526 2786 2988
Size: 107 122 71 631 605 854 260 202
Cuts at: 2988 4753 136
Size: 1765 1525
Fragments arranged by size:
1765 1525 854 631 605 260 202 122
107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 2744 2822 2978 3559 2744
Size: 78 156 581 5327
Fragments arranged by size:
5327 581 156 78
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2222 2228 2268 2955 3074 4203 5359
Size: 330 102 6 40 687 119 1129 1156
Cuts at: 5359 1790
Size: 2573
Fragments arranged by size:
2573 1156 1129 687 330 119 102 40
6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3873 4831 442
Size: 416 53 83 399 24 2456 958 1753
Fragments arranged by size:
2456 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2693 3116 3371 3380 4802 1239
Size: 1454 423 255 9 1422 2579
Fragments arranged by size:
2579 1454 1422 423 255 9
Bpu10I CC'TnA_GC
Cuts at: 2248 2723 3173 2248
Size: 475 450 5217
Fragments arranged by size:
5217 475 450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 4267 5132 5430 2588
Size: 1679 865 298 3300
Fragments arranged by size:
3300 1679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2842 3103 3933 493
Size: 711 1638 261 830 2702
Fragments arranged by size:
2702 1638 830 711 261
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2529 2778 3949 4331
Size: 53 83 186 936 996 249 1171 382
Cuts at: 4331 4966 275
Size: 635 1451
Fragments arranged by size:
1451 1171 996 936 635 382 249 186
83 53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 1178
Size: 30 165 30 748 30 5139
Fragments arranged by size:
5139 748 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3143 3368 1186
Size: 1957 225 3960
Fragments arranged by size:
3960 1957 225
BseYI C'CCAG_C
Cuts at: 2177 2213 2269 2916 5345 5841 2177
Size: 36 56 647 2429 496 2478
Fragments arranged by size:
2478 2429 647 496 56 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3101 3356 1163
Size: 573 1365 255 3949
Fragments arranged by size:
3949 1365 573 255
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2282 3592 4353 5381 1540
Size: 275 186 281 1310 761 1028 2301
Fragments arranged by size:
2301 1310 1028 761 281 275 186
BsiHKAI G_wGCw'C
Cuts at: 2228 2442 2955 3024 3135 3141 3396 4203 4288
Size: 214 513 69 111 6 255 807 85
Cuts at: 4288 5359 2228
Size: 1071 3011
Fragments arranged by size:
3011 1071 807 513 255 214 111 85
69 6
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
BsmBI CGTCTCn'nnnn_
Cuts at: 2536 2536
Size: 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2120 2222 2228 2268 2319 2442
Size: 399 225 105 102 6 40 51 123
Cuts at: 2442 2955 3024 3074 3135 3141 3396 3461 4203
Size: 513 69 50 61 6 255 65 742
Cuts at: 4203 4288 5359 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 399 255 225 123
105 102 85 69 65 61 51 50
40 6 6
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 2490 3045 3123 3222 3426 3497 844
Size: 1381 265 555 78 99 204 71 3489
Fragments arranged by size:
3489 1381 555 265 204 99 78 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 4029 1394
Size: 429 455 1751 3507
Fragments arranged by size:
3507 1751 455 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2419 4786 4973 1700
Size: 110 609 2367 187 2869
Fragments arranged by size:
2869 2367 609 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 2727 2729 2873 1925
Size: 802 2 144 5194
Fragments arranged by size:
5194 802 144 2
BssSI C'ACGA_G
Cuts at: 3023 3064 3134 4202 5214 3023
Size: 41 70 1068 1012 3951
Fragments arranged by size:
3951 1068 1012 70 41
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2803 2899 2959 3418 794
Size: 2009 96 60 459 3518
Fragments arranged by size:
3518 2009 459 96 60
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 3001 1184
Size: 1817 4325
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 2092
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2304 3472 3839 4224 4241 5693 5779 5791
Size: 332 1168 367 385 17 1452 86 12
Cuts at: 5791 6137 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1168 385 367 346 332 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2292 2932 513
Size: 552 1227 640 3723
Fragments arranged by size:
3723 1227 640 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2767 3232 487
Size: 683 56 1541 465 3397
Fragments arranged by size:
3397 1541 683 465 56
BtsI GCAGTG_nn'
Cuts at: 3658 4452 4472 3658
Size: 794 20 5328
Fragments arranged by size:
5328 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DraIII CAC_nnn'GTG
Cuts at: 2938 2938
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2279 2289
Size: 54 1425 50 275 18 270 179 10
Cuts at: 2289 2769 2787 3027 3589 4478 6107 8
Size: 480 18 240 562 889 1629 43
Fragments arranged by size:
1629 1425 889 562 480 275 270 240
179 54 50 43 18 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2279 3589 1537
Size: 275 467 1310 4090
Fragments arranged by size:
4090 1310 467 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 3401 4072 1353
Size: 971 1077 671 3423
Fragments arranged by size:
3423 1077 971 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2831 4729 5103 5249 387
Size: 1064 138 1242 1898 374 146 1280
Fragments arranged by size:
1898 1280 1242 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2440 3022 3133 3394 2440
Size: 582 111 261 5188
Fragments arranged by size:
5188 582 261 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2693 3116 3269 3371 3380 4205 4802
Size: 393 1061 423 153 102 9 825 597
Cuts at: 4802 5589 5857 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1061 825 787 597 423 393 268
153 102 9
EcoO109I rG'GnC_Cy
Cuts at: 2218 2218
Size: 6142
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 3041 3050 4648 3041
Size: 9 1598 4535
Fragments arranged by size:
4535 1598 9
HaeII r_GCGC'y
Cuts at: 1678 2611 2858 3095 5289 1678
Size: 933 247 237 2194 2531
Fragments arranged by size:
2531 2194 933 247 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4783 4815 4857 4889 1373
Size: 32 135 32 3211 32 42 32 2626
Fragments arranged by size:
3211 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2944 3486 3732 669
Size: 988 281 1006 542 246 3079
Fragments arranged by size:
3079 1006 988 542 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2559 5256 5440 1305
Size: 1254 2697 184 2007
Fragments arranged by size:
2697 2007 1254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 10
Size: 54 1425 343 459 3861
Fragments arranged by size:
3861 1425 459 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 2765 3086 4100 4459 4618 518
Size: 648 1254 345 321 1014 359 159 2042
Fragments arranged by size:
2042 1254 1014 648 359 345 321 159
MspA1I CmG'CkG
Cuts at: 1606 2273 2287 2800 3304 3340 3426 3558 4235
Size: 667 14 513 504 36 86 132 677
Cuts at: 4235 5383 5628 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 667 513 504 245 132
86 36 14
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5590
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 2279 3589 2279
Size: 1310 4832
NspI r_CATG'y
Cuts at: 1132 1229 2432 3500 5045 1132
Size: 97 1203 1068 1545 2229
Fragments arranged by size:
2229 1545 1203 1068 97
PasI CC'CwG_GG
Cuts at: 1348 3265 1348
Size: 1917 4225
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2403 2938 1108
Size: 1295 535 4312
Fragments arranged by size:
4312 1295 535
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PmlI CAC'GTG
Cuts at: 2842 2842
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 2488 4211 4243 5724 5756 2456
Size: 32 1723 32 1481 32 2842
Fragments arranged by size:
2842 1723 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspOMI G'GGCC_C
Cuts at: 2218 2218
Size: 6142
PspXI vC'TCGA_Gb
Cuts at: 2633 2633
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2487 2832 3345 3494 830
Size: 1657 345 513 149 3478
Fragments arranged by size:
3478 1657 513 345 149
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2800 3340 2800
Size: 540 5602
SacI G_AGCT'C
Cuts at: 2442 3024 3135 3396 2442
Size: 582 111 261 5188
Fragments arranged by size:
5188 582 261 111
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SapI GCTCTTCn'nnn_
Cuts at: 2324 3401 2324
Size: 1077 5065
SbfI CC_TGCA'GG
Cuts at: 2487 2832 3494 2487
Size: 345 662 5135
Fragments arranged by size:
5135 662 345
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2483 2828 3341 3490 4625 5306 5497
Size: 219 1438 345 513 149 1135 681 191
Cuts at: 5497 826
Size: 1471
Fragments arranged by size:
1471 1438 1135 681 513 345 219 191
149
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 2419 1700
Size: 719 5423
SmaI CCC'GGG
Cuts at: 1872 2016 2201 3379 3469 1872
Size: 144 185 1178 90 4545
Fragments arranged by size:
4545 1178 185 144 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2603 2633 4246 5147 5409 820
Size: 197 926 660 30 1613 901 262 1553
Fragments arranged by size:
1613 1553 926 901 660 262 197 30
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 2432 3500 1132
Size: 1300 1068 3774
Fragments arranged by size:
3774 1300 1068
SrfI GCCC'GGGC
Cuts at: 2016 2016
Size: 6142
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 2372 2412 2631 2834 1517
Size: 855 40 219 203 4825
Fragments arranged by size:
4825 855 219 203 40
StyI C'CwwG_G
Cuts at: 513 1065 2112 2253 2433 2997 3581 513
Size: 552 1047 141 180 564 584 3074
Fragments arranged by size:
3074 1047 584 564 552 180 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 2176 2191 2283 4169 4186 4339
Size: 1255 504 92 15 92 1886 17 153
Cuts at: 4339 4524 4969 325
Size: 185 445 1498
Fragments arranged by size:
1886 1498 1255 504 445 185 153 92
92 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4388 6100 96
Size: 274 80 33 51 1443 2411 1712 138
Fragments arranged by size:
2411 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2151 2183 6102 6134 2151
Size: 32 3919 32 2159
Fragments arranged by size:
3919 2159 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2935 2935
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 2633 1943
Size: 690 5452
XmaI C'CCGG_G
Cuts at: 1870 2014 2199 3377 3467 1870
Size: 144 185 1178 90 4545
Fragments arranged by size:
4545 1178 185 144 90
XmnI GAAnn'nnTTC
Cuts at: 2471 4271 2471
Size: 1800 4342
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SwaI