Restriction map for
pFN21AE4108
(Circular) (Six-base) MAPSORT of: pFN21AE4108.seq Check: 1705 from: 1 to: 6139
pFN21AE4108 6139 bp
With 182 enzymes: SgfI *
March 28, 2012 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2707 2707
Size: 6139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2991 3949 278
Size: 53 83 186 936 1455 958 2468
Fragments arranged by size:
2468 1455 958 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 2061 3460 2061
Size: 1399 4740
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3116 4202 5586 5854 1632
Size: 1484 1086 1384 268 1917
Fragments arranged by size:
1917 1484 1384 1086 268
AfeI AGC'GCT
Cuts at: 2216 2216
Size: 6139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 2666 3012 3075 5038 6015 1203
Size: 1463 346 63 1963 977 1327
Fragments arranged by size:
1963 1463 1327 977 346 63
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 2669 2999 3146 1166
Size: 1503 330 147 4159
Fragments arranged by size:
4159 1503 330 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 2521 3284 3394 3882 5454 2521
Size: 763 110 488 1572 3206
Fragments arranged by size:
3206 1572 763 488 110
ApaI G_GGCC'C
Cuts at: 2509 2954 2509
Size: 445 5694
ApaLI G'TGCA_C
Cuts at: 2406 3202 4196 5352 2406
Size: 796 994 1156 3193
Fragments arranged by size:
3193 1156 994 796
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 3041 3404 3464 1870
Size: 73 813 285 363 60 4545
Fragments arranged by size:
4545 813 363 285 73 60
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2061 2475 3460 4915 618
Size: 325 217 627 274 414 985 1455 1842
Fragments arranged by size:
1842 1455 985 627 414 325 274 217
BanII G_rGCy'C
Cuts at: 1391 2185 2257 2509 2694 2763 2902 2954 3458
Size: 794 72 252 185 69 139 52 504
Cuts at: 3458 1391
Size: 4072
Fragments arranged by size:
4072 794 504 252 185 139 72 69
52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2983 928
Size: 971 1084 4084
Fragments arranged by size:
4084 1084 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2443 2477 4330 4364 1903
Size: 34 506 34 1853 34 3678
Fragments arranged by size:
3678 1853 506 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 2484 1548
Size: 936 5203
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2504 4750 136
Size: 107 122 71 631 605 832 2246 1525
Fragments arranged by size:
2246 1525 832 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 2606 3062 3556 2606
Size: 456 494 5189
Fragments arranged by size:
5189 494 456
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2233 2410 2509 2749 2954 3206 3403
Size: 358 85 177 99 240 205 252 197
Cuts at: 3403 4200 5356 1790
Size: 797 1156 2573
Fragments arranged by size:
2573 1156 797 358 252 240 205 197
177 99 85
BmgBI CAC'GTC
Cuts at: 1318 2083 2353 1318
Size: 765 270 5104
Fragments arranged by size:
5104 765 270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3870 4828 442
Size: 416 53 83 399 24 2453 958 1753
Fragments arranged by size:
2453 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3325
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2636 4799 1239
Size: 851 546 2163 2579
Fragments arranged by size:
2579 2163 851 546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3212 4264 5129 5427 3212
Size: 1052 865 298 3924
Fragments arranged by size:
3924 1052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4802 882
Size: 617 3303 2219
Fragments arranged by size:
3303 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2411 2667 3001 3343 3930 493
Size: 711 1207 256 334 342 587 2702
Fragments arranged by size:
2702 1207 711 587 342 334 256
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2651 2988 3946 4328
Size: 53 83 186 936 1118 337 958 382
Cuts at: 4328 4963 275
Size: 635 1451
Fragments arranged by size:
1451 1118 958 936 635 382 337 186
83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2609 2639 1178
Size: 30 165 30 1206 30 4678
Fragments arranged by size:
4678 1206 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2945 3287 3392 1186
Size: 979 780 342 105 3933
Fragments arranged by size:
3933 979 780 342 105
BseYI C'CCAG_C
Cuts at: 2956 5342 5838 2956
Size: 2386 496 3257
Fragments arranged by size:
3257 2386 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3377 1163
Size: 573 1641 3925
Fragments arranged by size:
3925 1641 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3589 4350 5378 1540
Size: 275 186 1111 477 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 477 275 186
BsiHKAI G_wGCw'C
Cuts at: 2410 2763 3006 3206 4200 4285 5356 2410
Size: 353 243 200 994 85 1071 3193
Fragments arranged by size:
3193 1071 994 353 243 200 85
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
BsmBI CGTCTCn'nnnn_
Cuts at: 2073 3296 2073
Size: 1223 4916
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2185 2233 2257 2410 2509 2694
Size: 399 358 37 48 24 153 99 185
Cuts at: 2694 2749 2763 2902 2954 3006 3206 3403 3458
Size: 55 14 139 52 52 200 197 55
Cuts at: 3458 4200 4285 5356 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 200 197 185
153 139 99 85 55 55 52 52
48 37 24 14
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2263 2610 2707 3090 3420 3494 844
Size: 1229 190 347 97 383 330 74 3489
Fragments arranged by size:
3489 1229 383 347 330 190 97 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3244 4026 1394
Size: 429 1421 782 3507
Fragments arranged by size:
3507 1421 782 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2591 4634 4808 65
Size: 1183 1343 2043 174 1396
Fragments arranged by size:
2043 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4783 4970 1700
Size: 110 2973 187 2869
Fragments arranged by size:
2973 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2523 3015 96
Size: 2427 492 3220
Fragments arranged by size:
3220 2427 492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 2147 3416 4199 5211 2147
Size: 1269 783 1012 3075
Fragments arranged by size:
3075 1269 1012 783
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2414 3394 794
Size: 1620 980 3539
Fragments arranged by size:
3539 1620 980
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 3430 2434
Size: 996 5143
BstYI r'GATC_y
Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134
Size: 1497 367 385 17 1452 86 12 346
Cuts at: 6134 1972
Size: 1977
Fragments arranged by size:
1977 1497 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2944 2944
Size: 6139
BtgI C'CryG_G
Cuts at: 513 1065 2004 2493 513
Size: 552 939 489 4159
Fragments arranged by size:
4159 939 552 489
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2375 487
Size: 683 56 1149 4251
Fragments arranged by size:
4251 1149 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3655 4449 4469 2890
Size: 765 794 20 4560
Fragments arranged by size:
4560 794 765 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DraIII CAC_nnn'GTG
Cuts at: 2237 2414 2237
Size: 177 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2577 2736
Size: 54 1425 50 275 18 342 405 159
Cuts at: 2736 2878 3109 3165 3216 3345 3586 4475 6104
Size: 142 231 56 51 129 241 889 1629
Cuts at: 6104 8
Size: 43
Fragments arranged by size:
1629 1425 889 405 342 275 241 231
159 142 129 56 54 51 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 3586 1537
Size: 275 1297 477 4090
Fragments arranged by size:
4090 1297 477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3092 3179 4069 1353
Size: 1739 87 890 3423
Fragments arranged by size:
3423 1739 890 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2237 3167 4726 5100 5246 387
Size: 1064 138 648 930 1559 374 146 1280
Fragments arranged by size:
1559 1280 1064 930 648 374 146 138
EcoICRI GAG'CTC
Cuts at: 2761 2761
Size: 6139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2636 3116 4202 4799 5586 5854
Size: 393 458 546 480 1086 597 787 268
Cuts at: 5854 1239
Size: 1524
Fragments arranged by size:
1524 1086 787 597 546 480 458 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2290 2717 2290
Size: 427 5712
EcoO109I rG'GnC_Cy
Cuts at: 2505 2506 2599 2950 2951 3409 2505
Size: 1 93 351 1 458 5235
Fragments arranged by size:
5235 458 351 93 1 1
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2218 2575 2732 3372 5286 1678
Size: 540 357 157 640 1914 2531
Fragments arranged by size:
2531 1914 640 540 357 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 4780 4812 4854
Size: 32 135 32 1049 32 2127 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 2127 1049 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 2065 3464 2065
Size: 1399 4740
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5253 5437 1305
Size: 3948 184 2007
Fragments arranged by size:
3948 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2579 3167 3218 3347
Size: 54 1425 343 342 405 588 51 129
Cuts at: 3347 10
Size: 2802
Fragments arranged by size:
2802 1425 588 405 343 342 129 54
51
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 2669 2999 3146 4097 4456 4615
Size: 648 1393 110 330 147 951 359 159
Cuts at: 4615 518
Size: 2042
Fragments arranged by size:
2042 1393 951 648 359 330 159 147
110
MspA1I CmG'CkG
Cuts at: 1606 2031 2187 2323 2593 2814 3281 3319 3555
Size: 425 156 136 270 221 467 38 236
Cuts at: 3555 4232 5380 5625 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 467 425 270 245 236
221 156 136 38
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4648
Fragments arranged by size:
4648 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3109 3586 3109
Size: 477 5662
NspI r_CATG'y
Cuts at: 1132 1229 2434 2558 3016 3079 3497 5042 1132
Size: 97 1205 124 458 63 418 1545 2229
Fragments arranged by size:
2229 1545 1205 458 418 124 97 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 3012 3075 5038 3012
Size: 63 1963 4113
Fragments arranged by size:
4113 1963 63
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6139
PfoI T'CCnGG_A
Cuts at: 2715 2715
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PmlI CAC'GTG
Cuts at: 2667 3001 3343 2667
Size: 334 342 5463
Fragments arranged by size:
5463 342 334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2599 3409 2599
Size: 810 5329
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PspOMI G'GGCC_C
Cuts at: 2505 2950 2505
Size: 445 5694
PspXI vC'TCGA_Gb
Cuts at: 3404 3404
Size: 6139
PstI C_TGCA'G
Cuts at: 830 2036 2291 2517 2704 2849 3491 830
Size: 1206 255 226 187 145 642 3478
Fragments arranged by size:
3478 1206 642 255 226 187 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2031 2593 2814 2031
Size: 562 221 5356
Fragments arranged by size:
5356 562 221
SacI G_AGCT'C
Cuts at: 2763 2763
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SapI GCTCTTCn'nnn_
Cuts at: 3179 3179
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 2291 3491 2291
Size: 1200 4939
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SfcI C'TryA_G
Cuts at: 826 1045 2032 2287 2513 2700 2845 3364 3487
Size: 219 987 255 226 187 145 519 123
Cuts at: 3487 4622 5303 5494 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 987 681 519 255 226 219
191 187 145 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 2504 2504
Size: 6139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3191 3404 4243 5144 5406 820
Size: 197 926 1248 213 839 901 262 1553
Fragments arranged by size:
1553 1248 926 901 839 262 213 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 2867 3093 1517
Size: 1350 226 4563
Fragments arranged by size:
4563 1350 226
StyI C'CwwG_G
Cuts at: 513 1065 2004 3219 3578 513
Size: 552 939 1215 359 3074
Fragments arranged by size:
3074 1215 939 552 359
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2393 2493 3140 3416 4166 4183 4336
Size: 1255 813 100 647 276 750 17 153
Cuts at: 4336 4521 4966 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 813 750 647 445 276 185
153 100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2365 2523
Size: 274 80 33 51 1443 147 241 158
Cuts at: 2523 3015 3210 4385 6097 96
Size: 492 195 1175 1712 138
Fragments arranged by size:
1712 1443 1175 492 274 241 195 158
147 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2609 2641 6099 6131 2609
Size: 32 3458 32 2617
Fragments arranged by size:
3458 2617 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2354 1316
Size: 1038 5101
XbaI T'CTAG_A
Cuts at: 2329 3475 2329
Size: 1146 4993
XhoI C'TCGA_G
Cuts at: 1943 3404 1943
Size: 1461 4678
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 2378 4268 2378
Size: 1890 4249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2989 3947 276
Size: 53 83 186 936 1455 958 2468
Fragments arranged by size:
2468 1455 958 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII
SanDI SexAI SfoI SrfI SwaI XcmI