Restriction map for
pFN21AE4107
(Circular) (Six-base) MAPSORT of: pFN21AE4107.seq Check: 9280 from: 1 to: 6139
pFN21AE4107 6139 bp
With 182 enzymes: SgfI *
March 28, 2012 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3396 3396
Size: 6139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2922 3949 278
Size: 53 83 186 936 1386 1027 2468
Fragments arranged by size:
2468 1386 1027 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3411 3482 1937
Size: 1474 71 4594
Fragments arranged by size:
4594 1474 71
Acc65I G'GTAC_C
Cuts at: 2367 2520 3460 2367
Size: 153 940 5046
Fragments arranged by size:
5046 940 153
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4202 5586 5854 1632
Size: 2570 1384 268 1917
Fragments arranged by size:
2570 1917 1384 268
AfeI AGC'GCT
Cuts at: 2159 2159
Size: 6139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 5038 6015 1203
Size: 3835 977 1327
Fragments arranged by size:
3835 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3161 1184
Size: 1977 4162
AlwNI CAG_nnn'CTG
Cuts at: 2049 2170 2578 2653 3882 5454 2049
Size: 121 408 75 1229 1572 2734
Fragments arranged by size:
2734 1572 1229 408 121 75
ApaI G_GGCC'C
Cuts at: 2507 3038 2507
Size: 531 5608
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2831 3254 2831
Size: 423 5716
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 3037 3464 1870
Size: 73 690 404 427 4545
Fragments arranged by size:
4545 690 427 404 73
AvrII C'CTAG_G
Cuts at: 2009 2009
Size: 6139
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2222 2367 2520 2537
Size: 325 217 627 336 99 145 153 17
Cuts at: 2537 2621 2969 3460 4915 618
Size: 84 348 491 1455 1842
Fragments arranged by size:
1842 1455 627 491 348 336 325 217
153 145 99 84 17
BanII G_rGCy'C
Cuts at: 1391 2507 2633 3038 3458 1391
Size: 1116 126 405 420 4072
Fragments arranged by size:
4072 1116 420 405 126
BbeI G_GCGC'C
Cuts at: 2625 2973 2625
Size: 348 5791
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2341 3242 928
Size: 971 442 901 3825
Fragments arranged by size:
3825 971 901 442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2935 2969 4330 4364 1903
Size: 34 50 34 914 34 1361 34 3678
Fragments arranged by size:
3678 1361 914 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2603 2753 3042
Size: 107 122 71 631 605 931 150 289
Cuts at: 3042 3094 3264 4750 136
Size: 52 170 1486 1525
Fragments arranged by size:
1525 1486 931 631 605 289 170 150
122 107 71 52
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2225 2507 2540 3038 3140 4200 5356 1790
Size: 435 282 33 498 102 1060 1156 2573
Fragments arranged by size:
2573 1156 1060 498 435 282 102 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3870 4828 442
Size: 416 53 83 399 24 2453 958 1753
Fragments arranged by size:
2453 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2309 2708 2726 4799 1239
Size: 899 171 399 18 2073 2579
Fragments arranged by size:
2579 2073 899 399 171 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4802 882
Size: 617 3303 2219
Fragments arranged by size:
3303 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3930 493
Size: 711 2726 2702
Fragments arranged by size:
2726 2702 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 2919 2970 3129
Size: 53 83 186 936 1089 297 51 159
Cuts at: 3129 3191 3946 4328 4963 275
Size: 62 755 382 635 1451
Fragments arranged by size:
1451 1089 936 755 635 382 297 186
159 83 62 53 51
BsaWI w'CCGG_w
Cuts at: 3426 4572 4970 5244 5391 5802 3426
Size: 1146 398 274 147 411 3763
Fragments arranged by size:
3763 1146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5914
Fragments arranged by size:
5914 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3326 1186
Size: 2140 3999
BseYI C'CCAG_C
Cuts at: 2263 2716 3031 5342 5838 2263
Size: 453 315 2311 496 2564
Fragments arranged by size:
2564 2311 496 453 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2231 2255 2402 2882 3368 3374 1163
Size: 573 495 24 147 480 486 6 3928
Fragments arranged by size:
3928 573 495 486 480 147 24 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2598 2732 3007 3172 3176 3589
Size: 275 186 597 134 275 165 4 413
Cuts at: 3589 4350 5378 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 597 413 275 275 186
165 134 4
BsiHKAI G_wGCw'C
Cuts at: 2154 3081 4200 4285 5356 2154
Size: 927 1119 85 1071 2937
Fragments arranged by size:
2937 1119 1071 927 85
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2225 2507 2540 2633 3038 3081
Size: 399 364 71 282 33 93 405 43
Cuts at: 3081 3140 3458 4200 4285 5356 1391
Size: 59 318 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 364 318 282
93 85 71 59 43 33
BspEI T'CCGG_A
Cuts at: 3426 5802 3426
Size: 2376 3763
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3351 3396 3494 844
Size: 2507 45 98 3489
Fragments arranged by size:
3489 2507 98 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2752 4026 1394
Size: 429 929 1274 3507
Fragments arranged by size:
3507 1274 929 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2788 3193 3317 4783 4970 1700
Size: 110 978 405 124 1466 187 2869
Fragments arranged by size:
2869 1466 978 405 187 124 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 2479 2481 2724 2761 2813 2831 3155 3254
Size: 554 2 243 37 52 18 324 99
Cuts at: 3254 1925
Size: 4810
Fragments arranged by size:
4810 554 324 243 99 52 37 18
2
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 2653 794
Size: 1376 483 4280
Fragments arranged by size:
4280 1376 483
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 2077 3064 1184
Size: 893 987 4259
Fragments arranged by size:
4259 987 893
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134
Size: 1497 367 385 17 1452 86 12 346
Cuts at: 6134 1972
Size: 1977
Fragments arranged by size:
1977 1497 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3412 3412
Size: 6139
BtgI C'CryG_G
Cuts at: 513 1065 2598 2742 2836 2844 2975 3132 3176
Size: 552 1533 144 94 8 131 157 44
Cuts at: 3176 513
Size: 3476
Fragments arranged by size:
3476 1533 552 157 144 131 94 44
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2208 487
Size: 683 56 982 4418
Fragments arranged by size:
4418 982 683 56
BtsI GCAGTG_nn'
Cuts at: 3138 3655 4449 4469 3138
Size: 517 794 20 4808
Fragments arranged by size:
4808 794 517 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DraIII CAC_nnn'GTG
Cuts at: 2616 2616
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 2466 2595
Size: 54 1425 50 275 18 597 39 129
Cuts at: 2595 2604 2729 2745 2850 3043 3169 3195 3586
Size: 9 125 16 105 193 126 26 391
Cuts at: 3586 4475 6104 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 597 391 275 193 129
126 125 105 54 50 43 39 26
18 16 9
EagI C'GGCC_G
Cuts at: 1537 1812 2595 2729 3169 3586 1537
Size: 275 783 134 440 417 4090
Fragments arranged by size:
4090 783 440 417 275 134
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 4069 1353
Size: 1733 983 3423
Fragments arranged by size:
3423 1733 983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3269 4726 5100 5246 387
Size: 1064 138 1680 1457 374 146 1280
Fragments arranged by size:
1680 1457 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2309 2708 2726 4202 4799 5586
Size: 393 506 171 399 18 1476 597 787
Cuts at: 5586 5854 1239
Size: 268 1524
Fragments arranged by size:
1524 1476 787 597 506 399 393 268
171 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2013 2864 2013
Size: 851 5288
EcoO109I rG'GnC_Cy
Cuts at: 2034 2551 2034
Size: 517 5622
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2161 2625 2759 2934 2973 5286 1678
Size: 483 464 134 175 39 2313 2531
Fragments arranged by size:
2531 2313 483 464 175 134 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4780 4812 4854 4886 1373
Size: 32 135 32 3208 32 42 32 2626
Fragments arranged by size:
3208 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2923 3483 3729 669
Size: 988 281 985 560 246 3079
Fragments arranged by size:
3079 988 985 560 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KasI G'GCGC_C
Cuts at: 2621 2969 2621
Size: 348 5791
KpnI G_GTAC'C
Cuts at: 2371 2524 3464 2371
Size: 153 940 5046
Fragments arranged by size:
5046 940 153
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5253 5437 1305
Size: 3948 184 2007
Fragments arranged by size:
3948 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 3045 10
Size: 54 1425 343 597 616 3104
Fragments arranged by size:
3104 1425 616 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4097 4456 4615 518
Size: 648 2931 359 159 2042
Fragments arranged by size:
2931 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2345 2797 2806 2846 2896 3031 3134
Size: 514 225 452 9 40 50 135 103
Cuts at: 3134 3178 3204 3349 3555 4232 5380 5625 1606
Size: 44 26 145 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 514 452 245 225 206
145 135 103 50 44 40 26 9
NaeI GCC'GGC
Cuts at: 3195 3319 3195
Size: 124 6015
NarI GG'CG_CC
Cuts at: 2622 2970 2622
Size: 348 5791
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5587
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 3193 3317 3193
Size: 124 6015
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 2729 3586 2729
Size: 857 5282
NspI r_CATG'y
Cuts at: 1132 1229 2479 3497 5042 1132
Size: 97 1250 1018 1545 2229
Fragments arranged by size:
2229 1545 1250 1018 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4543
PfoI T'CCnGG_A
Cuts at: 2286 2286
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2034 2034
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PspOMI G'GGCC_C
Cuts at: 2503 3034 2503
Size: 531 5608
PstI C_TGCA'G
Cuts at: 830 2325 2457 2574 2679 2811 2868 3348 3491
Size: 1495 132 117 105 132 57 480 143
Cuts at: 3491 830
Size: 3478
Fragments arranged by size:
3478 1495 480 143 132 132 117 105
57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2120 2797 2806 3031 2120
Size: 677 9 225 5228
Fragments arranged by size:
5228 677 225 9
SacII CC_GC'GG
Cuts at: 2847 3135 3179 2847
Size: 288 44 5807
Fragments arranged by size:
5807 288 44
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SapI GCTCTTCn'nnn_
Cuts at: 3086 3086
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SexAI A'CCwGG_T
Cuts at: 2667 3366 2667
Size: 699 5440
SfcI C'TryA_G
Cuts at: 826 1045 2321 2453 2570 2675 2807 2864 3289
Size: 219 1276 132 117 105 132 57 425
Cuts at: 3289 3344 3487 4622 5303 5494 826
Size: 55 143 1135 681 191 1471
Fragments arranged by size:
1471 1276 1135 681 425 219 191 143
132 132 117 105 57 55
SfiI GGCCn_nnn'nGGCC
Cuts at: 2603 3042 2603
Size: 439 5700
SfoI GGC'GCC
Cuts at: 2623 2971 2623
Size: 348 5791
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 5144 5406 820
Size: 197 926 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 2870 1517
Size: 1194 159 4786
Fragments arranged by size:
4786 1194 159
StyI C'CwwG_G
Cuts at: 513 1065 2009 2064 3435 3578 513
Size: 552 944 55 1371 143 3074
Fragments arranged by size:
3074 1371 944 552 143 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 4166 4183 4336 4521 4966 325
Size: 1255 516 2070 17 153 185 445 1498
Fragments arranged by size:
2070 1498 1255 516 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 4385 6097
Size: 274 80 33 51 1443 396 2012 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
2012 1712 1443 396 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2784 2816 6099 6131 2784
Size: 32 3283 32 2792
Fragments arranged by size:
3283 2792 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2062
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2920 3947 276
Size: 53 83 186 936 1386 1027 2468
Fragments arranged by size:
2468 1386 1027 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI
Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SanDI SrfI
SwaI