Restriction map for
pFN21AE4105
(Circular) (Six-base) MAPSORT of: pFN21AE4105.seq Check: 9842 from: 1 to: 6130
pFN21AE4105 6130 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2312 3363 3473 1937
Size: 375 1051 110 4594
Fragments arranged by size:
4594 1051 375 110
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3089 4193 5577 5845 1632
Size: 1457 1104 1384 268 1917
Fragments arranged by size:
1917 1457 1384 1104 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2663 2742 1184
Size: 1479 79 4572
Fragments arranged by size:
4572 1479 79
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 6098
AlwNI CAG_nnn'CTG
Cuts at: 2072 2377 3873 5445 2072
Size: 305 1496 1572 2757
Fragments arranged by size:
2757 1572 1496 305
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 2020 2047 2348 2835 2875 3019 3439 3665
Size: 527 27 301 487 40 144 420 226
Cuts at: 3665 3817 5926 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 527 487 420 301 226 152
144 40 27
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 1870
Size: 73 1512 4545
Fragments arranged by size:
4545 1512 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2019 2052 2472 2505 2019
Size: 33 420 33 5644
Fragments arranged by size:
5644 420 33 33
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3451 4906 618
Size: 325 217 627 1664 1455 1842
Fragments arranged by size:
1842 1664 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3387 3449 1391
Size: 1996 62 4072
Fragments arranged by size:
4072 1996 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2794 3432 928
Size: 971 895 638 3626
Fragments arranged by size:
3626 971 895 638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4321 4355 1903
Size: 34 50 34 2300 34 3678
Fragments arranged by size:
3678 2300 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BfrBI ATG'CAT
Cuts at: 2907 2907
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3408 6125 3408
Size: 2717 3413
BlpI GC'TnA_GC
Cuts at: 2117 3381 3547 2117
Size: 1264 166 4700
Fragments arranged by size:
4700 1264 166
Bme1580I G_kGCm'C
Cuts at: 1790 2400 4191 5347 1790
Size: 610 1791 1156 2573
Fragments arranged by size:
2573 1791 1156 610
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2403 3861 4819
Size: 416 53 83 399 24 986 1458 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1753 1458 986 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4790 1239
Size: 3551 2579
Bpu10I CC'TnA_GC
Cuts at: 3093 3093
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4255 5120 5418 4255
Size: 865 298 4967
Fragments arranged by size:
4967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3059 4563 4961 5235 5382 5793 3059
Size: 1504 398 274 147 411 3396
Fragments arranged by size:
3396 1504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3180 3210 1178
Size: 30 165 30 1777 30 4098
Fragments arranged by size:
4098 1777 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6130
BseYI C'CCAG_C
Cuts at: 5333 5829 5333
Size: 496 5634
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2837 1163
Size: 573 1101 4456
Fragments arranged by size:
4456 1101 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2431 2694 3067 3387 4191 4276 5347 2431
Size: 263 373 320 804 85 1071 3214
Fragments arranged by size:
3214 1071 804 373 320 263 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2400 2431 2694 3067 3387 3449 4191
Size: 399 610 31 263 373 320 62 742
Cuts at: 4191 4276 5347 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 610 399 373 320 263
85 62 31
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3100 4017 1394
Size: 429 1277 917 3507
Fragments arranged by size:
3507 1277 917 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2909 4625 4799 65
Size: 1183 1661 1716 174 1396
Fragments arranged by size:
1716 1661 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3187 96
Size: 3091 3039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2630 3408 3460 3827 4212 4229 5681 5767
Size: 658 778 52 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 778 658 385 367 346 86
52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2034 2888 513
Size: 552 969 854 3755
Fragments arranged by size:
3755 969 854 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 3646 4440 4460 3646
Size: 794 20 5316
Fragments arranged by size:
5316 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 2860 2860
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3255 3577 4466
Size: 54 1425 50 275 18 1425 322 889
Cuts at: 4466 6095 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 322 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3003 3107 4060 1353
Size: 1650 104 953 3423
Fragments arranged by size:
3423 1650 953 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3192 4717 5091 5237 387
Size: 1064 138 1603 1525 374 146 1280
Fragments arranged by size:
1603 1525 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3385 3385
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3089 4193 4790 5577 5845 1239
Size: 393 1457 1104 597 787 268 1524
Fragments arranged by size:
1524 1457 1104 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2047 3439 3817 2047
Size: 1392 378 4360
Fragments arranged by size:
4360 1392 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 5277 1678
Size: 3599 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2982 3014 4771 4803 4845
Size: 32 135 32 1410 32 1757 32 42
Cuts at: 4845 4877 1373
Size: 32 2626
Fragments arranged by size:
2626 1757 1410 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3001 3474 3720 669
Size: 988 281 1063 473 246 3079
Fragments arranged by size:
3079 1063 988 473 281 246
HindIII A'AGCT_T
Cuts at: 748 3289 748
Size: 2541 3589
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 2565 3729 2565
Size: 1164 4966
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5244 5428 1305
Size: 3939 184 2007
Fragments arranged by size:
3939 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3257 10
Size: 54 1425 343 1425 2883
Fragments arranged by size:
2883 1425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2724 4088 4447 4606 518
Size: 648 1558 1364 359 159 2042
Fragments arranged by size:
2042 1558 1364 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3546 4223 5371 5616 1606
Size: 1940 677 1148 245 2120
Fragments arranged by size:
2120 1940 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5578
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NsiI A_TGCA'T
Cuts at: 2909 2909
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 3488 5033 1132
Size: 97 2259 1545 2229
Fragments arranged by size:
2259 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PfoI T'CCnGG_A
Cuts at: 2318 3081 2318
Size: 763 5367
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 3482 830
Size: 2652 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
SacI G_AGCT'C
Cuts at: 3387 3387
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SapI GCTCTTCn'nnn_
Cuts at: 3107 3107
Size: 6130
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2510 3478 4613 5294 5485 826
Size: 219 1465 968 1135 681 191 1471
Fragments arranged by size:
1471 1465 1135 968 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4234 5135 5397 820
Size: 197 926 2291 901 262 1553
Fragments arranged by size:
2291 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2059 2445 3569 513
Size: 552 994 386 1124 3074
Fragments arranged by size:
3074 1124 994 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4157 4174 4327 4512 4957 325
Size: 1255 2577 17 153 185 445 1498
Fragments arranged by size:
2577 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3187 3399 4376
Size: 274 80 33 51 1443 1210 212 977
Cuts at: 4376 6088 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1210 977 274 212 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 6090 6122 2714
Size: 32 3344 32 2722
Fragments arranged by size:
3344 2722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 2932 3466 2932
Size: 534 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3289 3289
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 2791 4259 2791
Size: 1468 4662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI