Restriction map for
pFN21AE4103
(Circular) (Six-base) MAPSORT of: pFN21AE4103.seq Check: 7184 from: 1 to: 6121
pFN21AE4103 6121 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3106 3298 4184 5568 5836 1632
Size: 1474 192 886 1384 268 1917
Fragments arranged by size:
1917 1474 1384 886 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 2622 5020 5997 1203
Size: 1419 2398 977 1327
Fragments arranged by size:
2398 1419 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 2262 1166
Size: 1096 5025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 2479 2638 3295 3864 5436 2479
Size: 159 657 569 1572 3164
Fragments arranged by size:
3164 1572 657 569 159
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2684 2993 3203 3233 3430 3656 3808 5917
Size: 1191 309 210 30 197 226 152 2109
Cuts at: 5917 1493
Size: 1697
Fragments arranged by size:
2109 1697 1191 309 226 210 197 152
30
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 2880 3446 1870
Size: 73 937 566 4545
Fragments arranged by size:
4545 937 566 73
BamHI G'GATC_C
Cuts at: 2126 3451 3818 2126
Size: 1325 367 4429
Fragments arranged by size:
4429 1325 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3442 4897 618
Size: 325 217 627 1655 1455 1842
Fragments arranged by size:
1842 1655 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2948 3440 1391
Size: 1557 492 4072
Fragments arranged by size:
4072 1557 492
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2990 3304 928
Size: 971 1091 314 3745
Fragments arranged by size:
3745 1091 971 314
BbvCI CC'TCA_GC
Cuts at: 2795 2795
Size: 6121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4312 4346 1903
Size: 34 50 34 2291 34 3678
Fragments arranged by size:
3678 2291 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BfrBI ATG'CAT
Cuts at: 2227 2227
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3411 4732 136
Size: 107 122 71 631 605 1739 1321 1525
Fragments arranged by size:
1739 1525 1321 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2410 3282 6116 2410
Size: 872 2834 2415
Fragments arranged by size:
2834 2415 872
BlpI GC'TnA_GC
Cuts at: 2501 3538 2501
Size: 1037 5084
Bme1580I G_kGCm'C
Cuts at: 1790 4182 5338 1790
Size: 2392 1156 2573
Fragments arranged by size:
2573 2392 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2942 3852 4810
Size: 416 53 83 399 24 1525 910 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1753 1525 958 910 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2675 4781 1239
Size: 1436 2106 2579
Fragments arranged by size:
2579 2106 1436
Bpu10I CC'TnA_GC
Cuts at: 2795 2795
Size: 6121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 3026 4246 5111 5409 2549
Size: 477 1220 865 298 3261
Fragments arranged by size:
3261 1220 865 477 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2776 3912 493
Size: 711 1572 1136 2702
Fragments arranged by size:
2702 1572 1136 711
BsaBI GATnn'nnATC
Cuts at: 2172 6115 2172
Size: 3943 2178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3276 4554 4952 5226 5373 5784 3276
Size: 1278 398 274 147 411 3613
Fragments arranged by size:
3613 1278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2894 2924 1178
Size: 30 165 30 1491 30 4375
Fragments arranged by size:
4375 1491 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2007 2108 2333 1186
Size: 821 101 225 4974
Fragments arranged by size:
4974 821 225 101
BseYI C'CCAG_C
Cuts at: 2631 5324 5820 2631
Size: 2693 496 2932
Fragments arranged by size:
2932 2693 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2723 1163
Size: 573 987 4561
Fragments arranged by size:
4561 987 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 4182 4267 5338 2274
Size: 1908 85 1071 3057
Fragments arranged by size:
3057 1908 1071 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2948 3440 4182 4267 5338 1391
Size: 399 484 674 492 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 492 484 399 85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3476 844
Size: 2632 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 4616 4790 65
Size: 1183 789 2579 174 1396
Fragments arranged by size:
2579 1396 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2470 3295 794
Size: 1676 825 3620
Fragments arranged by size:
3620 1676 825
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2126 2410 3282 3451 3818 4203 4220 5672
Size: 154 284 872 169 367 385 17 1452
Cuts at: 5672 5758 5770 6116 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 872 385 367 346 284 169
154 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5569
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 2050 2480 3637 4431 4451 2050
Size: 430 1157 794 20 3720
Fragments arranged by size:
3720 1157 794 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 3184 3216
Size: 54 1425 50 275 18 414 940 32
Cuts at: 3216 3568 4457 6086 8
Size: 352 889 1629 43
Fragments arranged by size:
1629 1425 940 889 414 352 275 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 3060 4051 1353
Size: 1080 627 991 3423
Fragments arranged by size:
3423 1080 991 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2675 3106 3298 4184 4781 5568 5836
Size: 393 1043 431 192 886 597 787 268
Cuts at: 5836 1239
Size: 1524
Fragments arranged by size:
1524 1043 886 787 597 431 393 268
192
EcoNI CCTnn'n_nnAGG
Cuts at: 3155 3155
Size: 6121
EcoO109I rG'GnC_Cy
Cuts at: 2710 3356 2710
Size: 646 5475
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
EcoRV GAT'ATC
Cuts at: 2596 2596
Size: 6121
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 4762 4794 4836
Size: 32 135 32 889 32 2269 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2626 2269 889 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2489 2769 748
Size: 1741 280 4100
Fragments arranged by size:
4100 1741 280
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 3186 3218 10
Size: 54 1425 343 414 940 32 2913
Fragments arranged by size:
2913 1425 940 414 343 54 32
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 2262 4079 4438 4597 518
Size: 648 1066 30 1817 359 159 2042
Fragments arranged by size:
2042 1817 1066 648 359 159 30
MspA1I CmG'CkG
Cuts at: 1606 2085 2638 3537 4214 5362 5607 1606
Size: 479 553 899 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 677 553 479 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5569
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NsiI A_TGCA'T
Cuts at: 2229 2229
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3479 5024 1132
Size: 97 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2710 3356 2710
Size: 646 5475
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PstI C_TGCA'G
Cuts at: 830 2077 3473 830
Size: 1247 1396 3478
Fragments arranged by size:
3478 1396 1247
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2085 2638 2085
Size: 553 5568
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SapI GCTCTTCn'nnn_
Cuts at: 3060 3060
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2073 2737 2811 3469 4604 5285 5476
Size: 219 1028 664 74 658 1135 681 191
Cuts at: 5476 826
Size: 1471
Fragments arranged by size:
1471 1135 1028 681 664 658 219 191
74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2564 3005 4225 5126 5388 820
Size: 197 926 621 441 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 621 441 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 3560 513
Size: 552 2495 3074
Fragments arranged by size:
3074 2495 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 4148 4165 4318 4503 4948 325
Size: 1255 761 1807 17 153 185 445 1498
Fragments arranged by size:
1807 1498 1255 761 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4367 6079 96
Size: 274 80 33 51 1443 2390 1712 138
Fragments arranged by size:
2390 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2316 2348 6081 6113 2316
Size: 32 3733 32 2324
Fragments arranged by size:
3733 2324 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 2364 2688 4250 2364
Size: 324 1562 4235
Fragments arranged by size:
4235 1562 324
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI