Restriction map for
pFN21AE4102
(Circular) (Six-base) MAPSORT of: pFN21AE4102.seq Check: 9956 from: 1 to: 6118
pFN21AE4102 6118 bp
With 182 enzymes: SgfI *
March 28, 2012 13:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2182 4181 5565 5833 1632
Size: 550 1999 1384 268 1917
Fragments arranged by size:
1999 1917 1384 550 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2744 1184
Size: 1560 4558
AlwNI CAG_nnn'CTG
Cuts at: 2314 3223 3410 3861 5433 2314
Size: 909 187 451 1572 2999
Fragments arranged by size:
2999 1572 909 451 187
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2430 2554 3427 3653 3805 5914 1493
Size: 937 124 873 226 152 2109 1697
Fragments arranged by size:
2109 1697 937 873 226 152 124
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
AvrII C'CTAG_G
Cuts at: 3022 3022
Size: 6118
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3439 4894 618
Size: 325 217 627 1652 1455 1842
Fragments arranged by size:
1842 1652 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2454 3437 1391
Size: 1063 983 4072
Fragments arranged by size:
4072 1063 983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2831 3107 928
Size: 971 932 276 3939
Fragments arranged by size:
3939 971 932 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2140 4010 5220 2140
Size: 1870 1210 3038
Fragments arranged by size:
3038 1870 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2335 2343 2902 6113 2335
Size: 8 559 3211 2340
Fragments arranged by size:
3211 2340 559 8
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2103 4179 5335 1790
Size: 313 2076 1156 2573
Fragments arranged by size:
2573 2076 1156 313
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 3067 3234
Size: 416 53 83 399 24 658 992 167
Cuts at: 3234 3849 4807 442
Size: 615 958 1753
Fragments arranged by size:
1753 992 958 658 615 416 399 167
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2231 4778 1239
Size: 845 147 2547 2579
Fragments arranged by size:
2579 2547 845 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2476 4243 5108 5406 2468
Size: 8 1767 865 298 3180
Fragments arranged by size:
3180 1767 865 298 8
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2272 2835 1186
Size: 868 218 563 4469
Fragments arranged by size:
4469 868 563 218
BseYI C'CCAG_C
Cuts at: 2211 3216 5321 5817 2211
Size: 1005 2105 496 2512
Fragments arranged by size:
2512 2105 1005 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2380 2750 1163
Size: 573 644 370 4531
Fragments arranged by size:
4531 644 573 370
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4179 4264 5335 4179
Size: 85 1071 4962
Fragments arranged by size:
4962 1071 85
BsmI GAATG_Cn'
Cuts at: 2290 3629 3722 2290
Size: 1339 93 4686
Fragments arranged by size:
4686 1339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2454 3437 4179 4264 5335 1391
Size: 399 313 351 983 742 85 1071 2174
Fragments arranged by size:
2174 1071 983 742 399 351 313 85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4613 4787 65
Size: 1183 3365 174 1396
Fragments arranged by size:
3365 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2351 96
Size: 2255 3863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 2140 2335 2343 2902 3448 3815 4200 4217
Size: 168 195 8 559 546 367 385 17
Cuts at: 4217 5669 5755 5767 6113 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 559 546 385 367 346 195
168 86 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4627
Fragments arranged by size:
4627 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 3634 4428 4448 3634
Size: 794 20 5304
Fragments arranged by size:
5304 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3046 3565 4454
Size: 54 1425 50 275 18 1216 519 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1216 889 519 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 2622 3176 4048 1353
Size: 974 295 554 872 3423
Fragments arranged by size:
3423 974 872 554 295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3168 4705 5079 5225 387
Size: 1064 138 1579 1537 374 146 1280
Fragments arranged by size:
1579 1537 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2182 2231 4181 4778 5565 5833
Size: 393 452 98 49 1950 597 787 268
Cuts at: 5833 1239
Size: 1524
Fragments arranged by size:
1950 1524 787 597 452 393 268 98
49
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2036
Size: 6118
EcoO109I rG'GnC_Cy
Cuts at: 3203 3203
Size: 6118
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2462 3131 2462
Size: 669 5449
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2708 2740 2887 2919 2991
Size: 32 135 32 1136 32 147 32 72
Cuts at: 2991 3023 3125 3157 4759 4791 4833 4865 1373
Size: 32 102 32 1602 32 42 32 2626
Fragments arranged by size:
2626 1602 1136 147 135 102 72 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3165 3717 3165
Size: 552 5566
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4076 4435 4594 518
Size: 648 2910 359 159 2042
Fragments arranged by size:
2910 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3534 4211 5359 5604 1606
Size: 1928 677 1148 245 2120
Fragments arranged by size:
2120 1928 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4627
Fragments arranged by size:
4627 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PfoI T'CCnGG_A
Cuts at: 2132 2132
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3057 4187 4219 5700 5732 3025
Size: 32 1130 32 1481 32 3411
Fragments arranged by size:
3411 1481 1130 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3203 3203
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3291 3688 3291
Size: 397 5721
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 2069 4366 6078 2069
Size: 2297 1712 2109
Fragments arranged by size:
2297 2109 1712
SfcI C'TryA_G
Cuts at: 826 1045 3466 4601 5282 5473 826
Size: 219 2421 1135 681 191 1471
Fragments arranged by size:
2421 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 2455 4222 5123 5385 820
Size: 197 926 504 8 1767 901 262 1553
Fragments arranged by size:
1767 1553 926 901 504 262 197 8
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 2852 4042 5
Size: 47 2800 1190 2081
Fragments arranged by size:
2800 2081 1190 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2004 2993 3022 3557 513
Size: 552 939 989 29 535 3074
Fragments arranged by size:
3074 989 939 552 535 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3263 4145 4162 4315 4500 4945
Size: 1255 1506 177 882 17 153 185 445
Cuts at: 4945 325
Size: 1498
Fragments arranged by size:
1506 1498 1255 882 445 185 177 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2351 4364
Size: 274 80 33 51 1443 90 284 2013
Cuts at: 4364 6076 96
Size: 1712 138
Fragments arranged by size:
2013 1712 1443 284 274 138 90 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2902 2934 3082 3114 6078 6110 2902
Size: 32 148 32 2964 32 2910
Fragments arranged by size:
2964 2910 148 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 2143 2338 2434 2899 3454 2143
Size: 195 96 465 555 4807
Fragments arranged by size:
4807 555 465 195 96
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 3373 4247 3373
Size: 874 5244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI