Restriction map for
pFN21AE4101
(Circular) (Six-base) MAPSORT of: pFN21AE4101.seq Check: 9406 from: 1 to: 6118
pFN21AE4101 6118 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2463 3461 1937
Size: 526 998 4594
Fragments arranged by size:
4594 998 526
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2514 3003 3342 4181 5565 5833 1632
Size: 882 489 339 839 1384 268 1917
Fragments arranged by size:
1917 1384 882 839 489 339 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 2308 5017 5994 1203
Size: 1105 2709 977 1327
Fragments arranged by size:
2709 1327 1105 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 3188 3359 3861 5433 3188
Size: 171 502 1572 3873
Fragments arranged by size:
3873 1572 502 171
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2164 2405 2512 3427 3653 3805 5914 1493
Size: 671 241 107 915 226 152 2109 1697
Fragments arranged by size:
2109 1697 915 671 241 226 152 107
AseI AT'TA_AT
Cuts at: 160 1032 2384 4672 160
Size: 872 1352 2288 1606
Fragments arranged by size:
2288 1606 1352 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3439 4894 618
Size: 325 217 627 1652 1455 1842
Fragments arranged by size:
1842 1652 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2830 3437 1391
Size: 1439 607 4072
Fragments arranged by size:
4072 1439 607
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3063 928
Size: 971 1164 3983
Fragments arranged by size:
3983 1164 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 4179 5335 1790
Size: 2389 1156 2573
Fragments arranged by size:
2573 2389 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3849 4807 442
Size: 416 53 83 399 24 2432 958 1753
Fragments arranged by size:
2432 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2474 1056
Size: 1418 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2374 4778 1239
Size: 1135 2404 2579
Fragments arranged by size:
2579 2404 1135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2304 3093 4243 5108 5406 2304
Size: 789 1150 865 298 3016
Fragments arranged by size:
3016 1150 865 789 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6118
BseYI C'CCAG_C
Cuts at: 2523 2836 5321 5817 2523
Size: 313 2485 496 2824
Fragments arranged by size:
2824 2485 496 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2578 3134 1163
Size: 573 842 556 4147
Fragments arranged by size:
4147 842 573 556
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3069 4179 4264 5335 3069
Size: 1110 85 1071 3852
Fragments arranged by size:
3852 1110 1071 85
BsmI GAATG_Cn'
Cuts at: 3229 3629 3722 3229
Size: 400 93 5625
Fragments arranged by size:
5625 400 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2023 2023
Size: 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2830 3069 3437 4179 4264 5335 1391
Size: 399 1040 239 368 742 85 1071 2174
Fragments arranged by size:
2174 1071 1040 742 399 368 239 85
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 2533 2898 4007 5737 2533
Size: 365 1109 1730 2914
Fragments arranged by size:
2914 1730 1109 365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2449 3473 844
Size: 1322 283 1024 3489
Fragments arranged by size:
3489 1322 1024 283
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2815 4613 4787 65
Size: 1183 1567 1798 174 1396
Fragments arranged by size:
1798 1567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 3161 3448 3815 4200 4217 5669 5755 5767
Size: 1189 287 367 385 17 1452 86 12
Cuts at: 5767 6113 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1189 385 367 346 287 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 3132 513
Size: 552 939 114 1014 3499
Fragments arranged by size:
3499 1014 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 3634 4428 4448 3382
Size: 252 794 20 5052
Fragments arranged by size:
5052 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 2137 3422 2137
Size: 1285 4833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2633 2853 5125 809
Size: 507 1317 220 2272 1802
Fragments arranged by size:
2272 1802 1317 507 220
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3079 3565 4454
Size: 54 1425 50 275 18 1249 486 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1249 889 486 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4048 1353
Size: 2695 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 2514 3003 3342 4181 4778 5565
Size: 393 742 140 489 339 839 597 787
Cuts at: 5565 5833 1239
Size: 268 1524
Fragments arranged by size:
1524 839 787 742 597 489 393 339
268 140
EcoO109I rG'GnC_Cy
Cuts at: 2023 2059 2023
Size: 36 6082
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2435 3309 2435
Size: 874 5244
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2817 2849 4759 4791 4833
Size: 32 135 32 1245 32 1910 32 42
Cuts at: 4833 4865 1373
Size: 32 2626
Fragments arranged by size:
2626 1910 1245 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2764 2858 3199 3462 3708 669
Size: 988 281 826 94 341 263 246 3079
Fragments arranged by size:
3079 988 826 341 281 263 246 94
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2281 2359 2548 3147 3154 3424 5232 5416
Size: 976 78 189 599 7 270 1808 184
Cuts at: 5416 1305
Size: 2007
Fragments arranged by size:
2007 1808 976 599 270 189 184 78
7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3081 10
Size: 54 1425 343 1249 3047
Fragments arranged by size:
3047 1425 1249 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 4076 4435 4594 518
Size: 648 1147 1763 359 159 2042
Fragments arranged by size:
2042 1763 1147 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3118 3534 4211 5359 5604 1606
Size: 1512 416 677 1148 245 2120
Fragments arranged by size:
2120 1512 1148 677 416 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2118 3132 513
Size: 552 939 114 1014 3499
Fragments arranged by size:
3499 1014 939 552 114
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 2470 1052
Size: 1418 4700
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 2312 3476 5021 1132
Size: 97 1083 1164 1545 2229
Fragments arranged by size:
2229 1545 1164 1083 97
PacI TTA_AT'TAA
Cuts at: 2388 2388
Size: 6118
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 2308 5017 2308
Size: 2709 3409
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2059
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 3118 3118
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 3466 4601 5282 5473 826
Size: 219 2421 1135 681 191 1471
Fragments arranged by size:
2421 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2319 3108 4222 5123 5385 820
Size: 197 926 376 789 1114 901 262 1553
Fragments arranged by size:
1553 1114 926 901 789 376 262 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 2114 4042 5
Size: 47 2062 1928 2081
Fragments arranged by size:
2081 2062 1928 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 2742 3132 3301 3557 513
Size: 552 939 114 624 390 169 256 3074
Fragments arranged by size:
3074 939 624 552 390 256 169 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4145 4162 4315 4500 4945 325
Size: 1255 2565 17 153 185 445 1498
Fragments arranged by size:
2565 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 2924 4364
Size: 274 80 33 51 1443 69 878 1440
Cuts at: 4364 6076 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1440 878 274 138 80 69
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2295 2327 6078 6110 2295
Size: 32 3751 32 2303
Fragments arranged by size:
3751 2303 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2223 3130 2223
Size: 907 5211
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PacI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI