Restriction map for
pFN21AE4095
(Circular) (Six-base) MAPSORT of: pFN21AE4095.seq Check: 7372 from: 1 to: 6109
pFN21AE4095 6109 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 3452 1937
Size: 72 1443 4594
Fragments arranged by size:
4594 1443 72
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2591 2765 2939 4172 5556 5824 1632
Size: 959 174 174 1233 1384 268 1917
Fragments arranged by size:
1917 1384 1233 959 268 174 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 5008 5985 1203
Size: 3805 977 1327
Fragments arranged by size:
3805 1327 977
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6109
AlwNI CAG_nnn'CTG
Cuts at: 2133 2260 2569 3171 3852 5424 2133
Size: 127 309 602 681 1572 2818
Fragments arranged by size:
2818 1572 681 602 309 127
ApaI G_GGCC'C
Cuts at: 2192 2192
Size: 6109
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 2852 3418 3644 3796 5905 1493
Size: 1359 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 1359 566 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 3434 1870
Size: 73 1491 4545
Fragments arranged by size:
4545 1491 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3430 4885 618
Size: 325 217 627 1643 1455 1842
Fragments arranged by size:
1842 1643 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2192 2755 3428 1391
Size: 801 563 673 4072
Fragments arranged by size:
4072 801 673 563
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3223 928
Size: 971 1324 3814
Fragments arranged by size:
3814 1324 971
BbvCI CC'TCA_GC
Cuts at: 2397 2636 2397
Size: 239 5870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2671 2705 4300 4334 1903
Size: 34 734 34 1595 34 3678
Fragments arranged by size:
3678 1595 734 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2017 3006 6104 2017
Size: 989 3098 2022
Fragments arranged by size:
3098 2022 989
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2192 4170 5326 1790
Size: 313 89 1978 1156 2573
Fragments arranged by size:
2573 1978 1156 313 89
BmgBI CAC'GTC
Cuts at: 1318 3202 3406 1318
Size: 1884 204 4021
Fragments arranged by size:
4021 1884 204
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 3040 3840
Size: 416 53 83 399 24 658 965 800
Cuts at: 3840 4798 442
Size: 958 1753
Fragments arranged by size:
1753 965 958 800 658 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2399 2435 2399
Size: 36 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2194 2371 2405 2579 4769 1239
Size: 955 177 34 174 2190 2579
Fragments arranged by size:
2579 2190 955 177 174 34
Bpu10I CC'TnA_GC
Cuts at: 2397 2463 2636 2678 2397
Size: 66 173 42 5828
Fragments arranged by size:
5828 173 66 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2692 2756 4234 5099 5397 2692
Size: 64 1478 865 298 3404
Fragments arranged by size:
3404 1478 865 298 64
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3181 4772 882
Size: 617 1682 1591 2219
Fragments arranged by size:
2219 1682 1591 617
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2247 4542 4940 5214 5361 5772 2247
Size: 2295 398 274 147 411 2584
Fragments arranged by size:
2584 2295 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5884
Fragments arranged by size:
5884 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2414 2594 2763 1186
Size: 928 300 180 169 4532
Fragments arranged by size:
4532 928 300 180 169
BseYI C'CCAG_C
Cuts at: 2133 2661 2878 2995 5312 5808 2133
Size: 528 217 117 2317 496 2434
Fragments arranged by size:
2434 2317 528 496 217 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5536
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3559 4320 5348 1540
Size: 275 186 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4170 4255 5326 4170
Size: 85 1071 4953
Fragments arranged by size:
4953 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3620 3713 3260
Size: 360 93 5656
Fragments arranged by size:
5656 360 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2192 2755 3428 4170 4255 5326
Size: 399 313 89 563 673 742 85 1071
Cuts at: 5326 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 673 563 399 313 89
85
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3464 844
Size: 2620 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4753 4940 1700
Size: 110 2943 187 2869
Fragments arranged by size:
2943 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 2900 4169 5181 2900
Size: 1269 1012 3828
Fragments arranged by size:
3828 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 2727 4913 2727
Size: 2186 3923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 2363 2363
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2017 2914 3006 3439 3806 4191 4208 5660
Size: 45 897 92 433 367 385 17 1452
Cuts at: 5660 5746 5758 6104 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 897 433 385 367 346 92
86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2033 2126 3326 2033
Size: 93 1200 4816
Fragments arranged by size:
4816 1200 93
BtgI C'CryG_G
Cuts at: 513 1065 2362 513
Size: 552 1297 4260
Fragments arranged by size:
4260 1297 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2218 487
Size: 683 56 992 4378
Fragments arranged by size:
4378 992 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 3625 4419 4439 2304
Size: 1321 794 20 3974
Fragments arranged by size:
3974 1321 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3147 3413 3147
Size: 266 5843
DraIII CAC_nnn'GTG
Cuts at: 2094 2094
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 3556 4445
Size: 54 1425 50 275 18 396 1330 889
Cuts at: 4445 6074 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1330 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2571 2607 2915 4039 1353
Size: 1218 36 308 1124 3423
Fragments arranged by size:
3423 1218 1124 308 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4696 5070 5216 387
Size: 1064 138 3107 374 146 1280
Fragments arranged by size:
3107 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2194 2371 2405 2579 2591 2765 2939
Size: 393 562 177 34 174 12 174 174
Cuts at: 2939 4172 4769 5556 5824 1239
Size: 1233 597 787 268 1524
Fragments arranged by size:
1524 1233 787 597 562 393 268 177
174 174 174 34 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 3189 2036
Size: 1153 4956
EcoO109I rG'GnC_Cy
Cuts at: 2178 2188 3124 3322 2178
Size: 10 936 198 4965
Fragments arranged by size:
4965 936 198 10
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2739 2739
Size: 6109
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 2318 2346 5256 1678
Size: 640 28 2910 2531
Fragments arranged by size:
2910 2531 640 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3116 3148 3230 3262 4750
Size: 32 135 32 1544 32 82 32 1488
Cuts at: 4750 4782 4824 4856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1544 1488 135 82 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 5223 5407 1305
Size: 1584 2334 184 2007
Fragments arranged by size:
2334 2007 1584 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 10
Size: 54 1425 343 396 3891
Fragments arranged by size:
3891 1425 396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 2831 4067 4426 4585 518
Size: 648 1389 276 1236 359 159 2042
Fragments arranged by size:
2042 1389 1236 648 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 3525 4202 5350 5595 1606
Size: 1919 677 1148 245 2120
Fragments arranged by size:
2120 1919 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2362 513
Size: 552 1297 4260
Fragments arranged by size:
4260 1297 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3467 5012 1132
Size: 97 2238 1545 2229
Fragments arranged by size:
2238 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6109
PfoI T'CCnGG_A
Cuts at: 2421 3234 2421
Size: 813 5296
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2178 3124 2178
Size: 946 5163
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PspOMI G'GGCC_C
Cuts at: 2188 2188
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2265 3461 830
Size: 1435 1196 3478
Fragments arranged by size:
3478 1435 1196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 2010 2261 3292 3457 4592 5273 5464
Size: 219 965 251 1031 165 1135 681 191
Cuts at: 5464 826
Size: 1471
Fragments arranged by size:
1471 1135 1031 965 681 251 219 191
165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2707 2735 4213 5114 5376 820
Size: 197 926 764 28 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 764 262 197 28
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 3331 1517
Size: 1814 4295
StyI C'CwwG_G
Cuts at: 513 1065 2181 2310 2362 3548 513
Size: 552 1116 129 52 1186 3074
Fragments arranged by size:
3074 1186 1116 552 129 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3071 4136 4153 4306 4491 4936 325
Size: 1255 1491 1065 17 153 185 445 1498
Fragments arranged by size:
1498 1491 1255 1065 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2470 4355 6067
Size: 274 80 33 51 1443 493 1885 1712
Cuts at: 6067 96
Size: 138
Fragments arranged by size:
1885 1712 1443 493 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6069 6101 6069
Size: 32 6077
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI