Restriction map for
pFN21AE4094
(Circular) (Six-base) MAPSORT of: pFN21AE4094.seq Check: 485 from: 1 to: 6025
pFN21AE4094 6025 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 3368 1937
Size: 810 621 4594
Fragments arranged by size:
4594 810 621
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 3093 4152 4525 1563
Size: 1530 1059 373 3063
Fragments arranged by size:
3063 1530 1059 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4088 5472 5740 1632
Size: 2456 1384 268 1917
Fragments arranged by size:
2456 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 2627 3316 1166
Size: 1461 689 3875
Fragments arranged by size:
3875 1461 689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3053 1184
Size: 1869 4156
AlwNI CAG_nnn'CTG
Cuts at: 2041 2521 2735 2981 3768 5340 2041
Size: 480 214 246 787 1572 2726
Fragments arranged by size:
2726 1572 787 480 246 214
ApaLI G'TGCA_C
Cuts at: 3132 4082 5238 3132
Size: 950 1156 3919
Fragments arranged by size:
3919 1156 950
ApoI r'AATT_y
Cuts at: 1493 2178 2351 3334 3560 3712 5821 1493
Size: 685 173 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 685 226 173 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 3350 1870
Size: 73 1407 4545
Fragments arranged by size:
4545 1407 73
BamHI G'GATC_C
Cuts at: 3355 3722 3355
Size: 367 5658
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2602 2629 3346 4801 618
Size: 325 217 627 815 27 717 1455 1842
Fragments arranged by size:
1842 1455 815 717 627 325 217 27
BanII G_rGCy'C
Cuts at: 1391 2565 3344 1391
Size: 1174 779 4072
Fragments arranged by size:
4072 1174 779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 928
Size: 971 433 4621
Fragments arranged by size:
4621 971 433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2381 2415 2944 2978 4216 4250 1903
Size: 34 444 34 529 34 1238 34 3678
Fragments arranged by size:
3678 1238 529 444 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3165 3442 3165
Size: 277 5748
Bme1580I G_kGCm'C
Cuts at: 1790 3136 4086 5242 1790
Size: 1346 950 1156 2573
Fragments arranged by size:
2573 1346 1156 950
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3169 3756 4714
Size: 416 53 83 399 24 1752 587 958
Cuts at: 4714 442
Size: 1753
Fragments arranged by size:
1753 1752 958 587 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2543 3244 4685 1239
Size: 1304 701 1441 2579
Fragments arranged by size:
2579 1441 1304 701
Bpu10I CC'TnA_GC
Cuts at: 2709 2709
Size: 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4688 882
Size: 617 3189 2219
Fragments arranged by size:
3189 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3832 4214 4849 275
Size: 53 83 186 936 2299 382 635 1451
Fragments arranged by size:
2299 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3102 3132 1178
Size: 30 165 30 1699 30 4071
Fragments arranged by size:
4071 1699 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 5112
BseYI C'CCAG_C
Cuts at: 2704 5228 5724 2704
Size: 2524 496 3005
Fragments arranged by size:
3005 2524 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2709 1163
Size: 573 973 4479
Fragments arranged by size:
4479 973 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3136 4086 4171 5242 3136
Size: 950 85 1071 3919
Fragments arranged by size:
3919 1071 950 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2565 3136 3344 4086 4171 5242 1391
Size: 399 775 571 208 742 85 1071 2174
Fragments arranged by size:
2174 1071 775 742 571 399 208 85
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2695 3233 3380 844
Size: 1851 538 147 3489
Fragments arranged by size:
3489 1851 538 147
BsrBI CCG'CTC
Cuts at: 1394 1823 3912 1394
Size: 429 2089 3507
Fragments arranged by size:
3507 2089 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2473 4669 4856 1700
Size: 110 663 2196 187 2869
Fragments arranged by size:
2869 2196 663 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2933 794
Size: 2139 3886
BstBI TT'CG_AA
Cuts at: 2323 4829 2323
Size: 2506 3519
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2572 2764 3355 3722 4107 4124 5576 5662
Size: 600 192 591 367 385 17 1452 86
Cuts at: 5662 5674 6020 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 600 591 385 367 346 192
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 2352 3541 4335 4355 2352
Size: 1189 794 20 4022
Fragments arranged by size:
4022 1189 794 20
ClaI AT'CG_AT
Cuts at: 1554 2954 1554
Size: 1400 4625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3250 3472 4361
Size: 54 1425 50 275 18 1420 222 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1420 889 275 222 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 3955 1353
Size: 1083 1519 3423
Fragments arranged by size:
3423 1519 1083
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 3244 4088 4685 5472 5740 1239
Size: 393 911 701 844 597 787 268 1524
Fragments arranged by size:
1524 911 844 787 701 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3240 3240
Size: 6025
EcoO109I rG'GnC_Cy
Cuts at: 2123 2897 2123
Size: 774 5251
EcoRI G'AATT_C
Cuts at: 2178 3334 3712 2178
Size: 1156 378 4491
Fragments arranged by size:
4491 1156 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2324 2324
Size: 6025
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 5172 1678
Size: 3494 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2694 4666 4698 4740
Size: 32 135 32 1090 32 1972 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 1972 1090 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 3369 3615 669
Size: 988 281 286 1145 246 3079
Fragments arranged by size:
3079 1145 988 286 281 246
HindIII A'AGCT_T
Cuts at: 748 2072 748
Size: 1324 4701
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5139 5323 1305
Size: 3834 184 2007
Fragments arranged by size:
3834 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3252 10
Size: 54 1425 343 1420 2783
Fragments arranged by size:
2783 1425 1420 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 3023 3035 3316 3983 4342 4501
Size: 648 1461 396 12 281 667 359 159
Cuts at: 4501 518
Size: 2042
Fragments arranged by size:
2042 1461 667 648 396 359 281 159
12
MspA1I CmG'CkG
Cuts at: 1606 2492 2735 2933 3441 4118 5266 5511 1606
Size: 886 243 198 508 677 1148 245 2120
Fragments arranged by size:
2120 1148 886 677 508 245 243 198
NaeI GCC'GGC
Cuts at: 2475 2475
Size: 6025
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NgoMIV G'CCGG_C
Cuts at: 2473 2473
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2263 3034 3383 4928 1132
Size: 97 1034 771 349 1545 2229
Fragments arranged by size:
2229 1545 1034 771 349 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PfoI T'CCnGG_A
Cuts at: 2138 2138
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2123 2897 2123
Size: 774 5251
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2505 2977 3244 3377 830
Size: 1675 472 267 133 3478
Fragments arranged by size:
3478 1675 472 267 133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2492 2735 2933 2492
Size: 243 198 5584
Fragments arranged by size:
5584 243 198
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SanDI GG'GwC_CC
Cuts at: 2897 2897
Size: 6025
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 2099 2878 4273 5985 2099
Size: 779 1395 1712 2139
Fragments arranged by size:
2139 1712 1395 779
SfcI C'TryA_G
Cuts at: 826 1045 2501 2748 2973 3240 3373 4508 5189
Size: 219 1456 247 225 267 133 1135 681
Cuts at: 5189 5380 826
Size: 191 1471
Fragments arranged by size:
1471 1456 1135 681 267 247 225 219
191 133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 2263 3034 3383 1132
Size: 1131 771 349 3774
Fragments arranged by size:
3774 1131 771 349
SspI AAT'ATT
Cuts at: 5 52 2146 3949 5
Size: 47 2094 1803 2081
Fragments arranged by size:
2094 2081 1803 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 3106 3464 513
Size: 552 939 1102 358 3074
Fragments arranged by size:
3074 1102 939 552 358
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2680 3119 4052 4069 4222 4407 4852
Size: 1255 1100 439 933 17 153 185 445
Cuts at: 4852 325
Size: 1498
Fragments arranged by size:
1498 1255 1100 933 445 439 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 2876 4271
Size: 274 80 33 51 1443 120 779 1395
Cuts at: 4271 5983 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1395 779 274 138 120 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 3102 3134 5985 6017 2134
Size: 32 936 32 2851 32 2142
Fragments arranged by size:
2851 2142 936 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3009 1316
Size: 1693 4332
XbaI T'CTAG_A
Cuts at: 2767 3361 2767
Size: 594 5431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3049 3146 3049
Size: 97 5928
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI