Restriction map for
pFN21AE4092
(Circular) (Six-base) MAPSORT of: pFN21AE4092.seq Check: 7356 from: 1 to: 6103
pFN21AE4092 6103 bp
With 182 enzymes: SgfI *
March 28, 2012 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3196 3196
Size: 6103
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2415 3913 278
Size: 53 83 186 936 879 1498 2468
Fragments arranged by size:
2468 1498 936 879 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2390 3446 1937
Size: 453 1056 4594
Fragments arranged by size:
4594 1056 453
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2710 2963 3088 3280 4166 5550 5818 1632
Size: 1078 253 125 192 886 1384 268 1917
Fragments arranged by size:
1917 1384 1078 886 268 253 192 125
AflII C'TTAA_G
Cuts at: 820 1017 2405 820
Size: 197 1388 4518
Fragments arranged by size:
4518 1388 197
AflIII A'CryG_T
Cuts at: 1203 3169 5002 5979 1203
Size: 1966 1833 977 1327
Fragments arranged by size:
1966 1833 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 2017 2152 2281 2479 2491 2626 3846 5418 2017
Size: 135 129 198 12 135 1220 1572 2702
Fragments arranged by size:
2702 1572 1220 198 135 135 129 12
ApaI G_GGCC'C
Cuts at: 2833 2833
Size: 6103
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 3013 3185 3412 3638 3790 5899 1493
Size: 1520 172 227 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 227 226 172 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 2232 2247 2307 2987 3428 1870
Size: 73 234 55 15 60 680 441 4545
Fragments arranged by size:
4545 680 441 234 73 60 55 15
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2400 2528 3424 4879
Size: 325 217 627 368 245 128 896 1455
Cuts at: 4879 618
Size: 1842
Fragments arranged by size:
1842 1455 896 627 368 325 245 217
128
BanII G_rGCy'C
Cuts at: 1391 2073 2213 2232 2241 2586 2833 2930 3341
Size: 682 140 19 9 345 247 97 411
Cuts at: 3341 3422 1391
Size: 81 4072
Fragments arranged by size:
4072 682 411 345 247 140 97 81
19 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2954 3404 928
Size: 971 1055 450 3627
Fragments arranged by size:
3627 1055 971 450
BbvCI CC'TCA_GC
Cuts at: 2051 2122 2051
Size: 71 6032
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4294 4328 1903
Size: 34 50 34 2273 34 3678
Fragments arranged by size:
3678 2273 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BfrBI ATG'CAT
Cuts at: 2461 2461
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2799 3380 6098 2799
Size: 581 2718 2804
Fragments arranged by size:
2804 2718 581
BlpI GC'TnA_GC
Cuts at: 2207 3520 2207
Size: 1313 4790
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2403 2833 4164 5320 1790
Size: 370 243 430 1331 1156 2573
Fragments arranged by size:
2573 1331 1156 430 370 243
BmgBI CAC'GTC
Cuts at: 1318 2857 3172 1318
Size: 1539 315 4249
Fragments arranged by size:
4249 1539 315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3834 4792 442
Size: 416 53 83 399 24 2417 958 1753
Fragments arranged by size:
2417 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 2768 2920 3212 4763 1239
Size: 1424 105 152 292 1551 2579
Fragments arranged by size:
2579 1551 1424 292 152 105
Bpu10I CC'TnA_GC
Cuts at: 2051 2122 2051
Size: 71 6032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4228 5093 5391 4228
Size: 865 298 4940
Fragments arranged by size:
4940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 4766 882
Size: 617 855 2412 2219
Fragments arranged by size:
2412 2219 855 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3894 493
Size: 711 1431 1259 2702
Fragments arranged by size:
2702 1431 1259 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2412 3258 3910 4292
Size: 53 83 186 936 879 846 652 382
Cuts at: 4292 4927 275
Size: 635 1451
Fragments arranged by size:
1451 936 879 846 652 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2313 2448 4536 4934 5208 5355 5766 2313
Size: 135 2088 398 274 147 411 2650
Fragments arranged by size:
2650 2088 411 398 274 147 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2876 2906 1178
Size: 30 165 30 1473 30 4375
Fragments arranged by size:
4375 1473 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2684 1186
Size: 1213 285 4605
Fragments arranged by size:
4605 1213 285
BseYI C'CCAG_C
Cuts at: 2090 2619 5306 5802 2090
Size: 529 2687 496 2391
Fragments arranged by size:
2687 2391 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 1163
Size: 573 729 4801
Fragments arranged by size:
4801 729 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3553 4314 5342 1540
Size: 275 186 1552 761 1028 2301
Fragments arranged by size:
2301 1552 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2213 2241 2586 2994 4164 4249 5320 2213
Size: 28 345 408 1170 85 1071 2996
Fragments arranged by size:
2996 1170 1071 408 345 85 28
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2160 2213 2232 2241 2403 2586
Size: 399 283 87 53 19 9 162 183
Cuts at: 2586 2833 2930 2994 3341 3422 4164 4249 5320
Size: 247 97 64 347 81 742 85 1071
Cuts at: 5320 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 347 283 247 183
162 97 87 85 81 64 53 19
9
BspEI T'CCGG_A
Cuts at: 2313 5766 2313
Size: 3453 2650
BspHI T'CATG_A
Cuts at: 3992 5722 3992
Size: 1730 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3196 3458 844
Size: 2352 262 3489
Fragments arranged by size:
3489 2352 262
BsrBI CCG'CTC
Cuts at: 1394 1823 2647 3057 3990 1394
Size: 429 824 410 933 3507
Fragments arranged by size:
3507 933 824 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2290 4747 4934 1700
Size: 110 480 2457 187 2869
Fragments arranged by size:
2869 2457 480 187 110
BsrGI T'GTAC_A
Cuts at: 96 3279 96
Size: 3183 2920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 794
Size: 1361 4742
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 2035 1184
Size: 851 5252
BstXI CCAn_nnnn'nTGG
Cuts at: 2746 3208 2746
Size: 462 5641
BstYI r'GATC_y
Cuts at: 1972 2799 3380 3433 3800 4185 4202 5654 5740
Size: 827 581 53 367 385 17 1452 86
Cuts at: 5740 5752 6098 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 827 581 385 367 346 86
53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2667 2667
Size: 6103
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2410 487
Size: 683 56 1184 4180
Fragments arranged by size:
4180 1184 683 56
BtsI GCAGTG_nn'
Cuts at: 3619 4413 4433 3619
Size: 794 20 5289
Fragments arranged by size:
5289 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DraIII CAC_nnn'GTG
Cuts at: 2155 2155
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2346 2706 2901
Size: 54 1425 50 275 18 516 360 195
Cuts at: 2901 2970 3198 3550 4439 6068 8
Size: 69 228 352 889 1629 43
Fragments arranged by size:
1629 1425 889 516 360 352 275 228
195 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 2447 4033 1353
Size: 872 222 1586 3423
Fragments arranged by size:
3423 1586 872 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2211 2239 2584 2211
Size: 28 345 5730
Fragments arranged by size:
5730 345 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2663 2710 2768 2920 2963 3088 3212
Size: 393 1031 47 58 152 43 125 124
Cuts at: 3212 3280 4166 4763 5550 5818 1239
Size: 68 886 597 787 268 1524
Fragments arranged by size:
1524 1031 886 787 597 393 268 152
125 124 68 58 47 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2368 3137 2368
Size: 769 5334
EcoO109I rG'GnC_Cy
Cuts at: 2698 2829 2698
Size: 131 5972
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 2117 5250 1678
Size: 439 3133 2531
Fragments arranged by size:
3133 2531 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2446 2478 4744 4776 4818
Size: 32 135 32 874 32 2266 32 42
Cuts at: 4818 4850 1373
Size: 32 2626
Fragments arranged by size:
2626 2266 874 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2391 3175 3447 3693 669
Size: 988 281 453 784 272 246 3079
Fragments arranged by size:
3079 988 784 453 281 272 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2052 5217 5401 1305
Size: 747 3165 184 2007
Fragments arranged by size:
3165 2007 747 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2708 2903 3200 10
Size: 54 1425 343 876 195 297 2913
Fragments arranged by size:
2913 1425 876 343 297 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 2921 4061 4420 4579 518
Size: 648 1689 66 1140 359 159 2042
Fragments arranged by size:
2042 1689 1140 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2281 2341 2626 3519 4196 5344 5589 1606
Size: 675 60 285 893 677 1148 245 2120
Fragments arranged by size:
2120 1148 893 677 675 285 245 60
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5551
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NsiI A_TGCA'T
Cuts at: 2463 2463
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2809 3461 5006 1132
Size: 97 1580 652 1545 2229
Fragments arranged by size:
2229 1580 1545 652 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 2491 1108
Size: 1044 339 4720
Fragments arranged by size:
4720 1044 339
PfoI T'CCnGG_A
Cuts at: 2103 2379 3189 2103
Size: 276 810 5017
Fragments arranged by size:
5017 810 276
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PmlI CAC'GTG
Cuts at: 2635 2635
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2698 2698
Size: 6103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PspOMI G'GGCC_C
Cuts at: 2829 2829
Size: 6103
PspXI vC'TCGA_Gb
Cuts at: 2987 2987
Size: 6103
PstI C_TGCA'G
Cuts at: 830 2340 2600 3455 830
Size: 1510 260 855 3478
Fragments arranged by size:
3478 1510 855 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2281 2341 2626 2281
Size: 60 285 5758
Fragments arranged by size:
5758 285 60
SacI G_AGCT'C
Cuts at: 2213 2241 2586 2213
Size: 28 345 5730
Fragments arranged by size:
5730 345 28
SalI G'TCGA_C
Cuts at: 1936 2389 3445 1936
Size: 453 1056 4594
Fragments arranged by size:
4594 1056 453
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SexAI A'CCwGG_T
Cuts at: 2151 2784 2151
Size: 633 5470
SfcI C'TryA_G
Cuts at: 826 1045 2336 2471 2548 2596 3025 3451 4586
Size: 219 1291 135 77 48 429 426 1135
Cuts at: 4586 5267 5458 826
Size: 681 191 1471
Fragments arranged by size:
1471 1291 1135 681 429 426 219 191
135 77 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 2234 2249 2309 3430 1872
Size: 362 15 60 1121 4545
Fragments arranged by size:
4545 1121 362 60 15
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 2987 4207 5108 5370 820
Size: 197 926 462 582 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 582 462 262 197
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 2809 3461 1132
Size: 1677 652 3774
Fragments arranged by size:
3774 1677 652
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 2684 1517
Size: 1167 4936
StyI C'CwwG_G
Cuts at: 513 1065 2369 2587 3542 513
Size: 552 1304 218 955 3074
Fragments arranged by size:
3074 1304 955 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2372 2516 3284 4130 4147 4300 4485
Size: 1255 792 144 768 846 17 153 185
Cuts at: 4485 4930 325
Size: 445 1498
Fragments arranged by size:
1498 1255 846 792 768 445 185 153
144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3279 4349 6061
Size: 274 80 33 51 1443 1302 1070 1712
Cuts at: 6061 96
Size: 138
Fragments arranged by size:
1712 1443 1302 1070 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6063 6095 6063
Size: 32 6071
Tth111I GACn'n_nGTC
Cuts at: 1316 2033 1316
Size: 717 5386
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 2987 1943
Size: 1044 5059
XmaI C'CCGG_G
Cuts at: 1870 2232 2247 2307 3428 1870
Size: 362 15 60 1121 4545
Fragments arranged by size:
4545 1121 362 60 15
XmnI GAAnn'nnTTC
Cuts at: 2765 4232 2765
Size: 1467 4636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2413 3911 276
Size: 53 83 186 936 879 1498 2468
Fragments arranged by size:
2468 1498 936 879 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI