Restriction map for
pFN21AE4091
(Circular) (Six-base) MAPSORT of: pFN21AE4091.seq Check: 1680 from: 1 to: 6079
pFN21AE4091 6079 bp
With 182 enzymes: SgfI *
March 28, 2012 13:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3129 4142 5526 5794 1632
Size: 1497 1013 1384 268 1917
Fragments arranged by size:
1917 1497 1384 1013 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 4978 5955 1203
Size: 3775 977 1327
Fragments arranged by size:
3775 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 2128 2504 2852 3145 3822 5394 2128
Size: 376 348 293 677 1572 2813
Fragments arranged by size:
2813 1572 677 376 348 293
ApaI G_GGCC'C
Cuts at: 3127 3127
Size: 6079
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 2635 3388 3614 3766 5875 1493
Size: 1142 753 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 753 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3404 1870
Size: 73 1461 4545
Fragments arranged by size:
4545 1461 73
BamHI G'GATC_C
Cuts at: 2381 3409 3776 2381
Size: 1028 367 4684
Fragments arranged by size:
4684 1028 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 4855 618
Size: 325 217 627 1613 1455 1842
Fragments arranged by size:
1842 1613 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 2454 3127 3398 1391
Size: 628 435 673 271 4072
Fragments arranged by size:
4072 673 628 435 271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2550 2584 2959 2993 4270 4304 1903
Size: 34 613 34 375 34 1277 34 3678
Fragments arranged by size:
3678 1277 613 375 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 2042 4690
Size: 107 122 71 631 605 358 12 2648
Cuts at: 4690 136
Size: 1525
Fragments arranged by size:
2648 1525 631 605 358 122 107 71
12
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 2573 2766 3127 4140 5296 1790
Size: 783 193 361 1013 1156 2573
Fragments arranged by size:
2573 1156 1013 783 361 193
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2255 3810 4768
Size: 416 53 83 399 24 838 1555 958
Cuts at: 4768 442
Size: 1753
Fragments arranged by size:
1753 1555 958 838 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 2228
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2836 3024 4739 1239
Size: 1597 188 1715 2579
Fragments arranged by size:
2579 1715 1597 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4204 5069 5367 4204
Size: 865 298 4916
Fragments arranged by size:
4916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2202 3022 4742 882
Size: 617 703 820 1720 2219
Fragments arranged by size:
2219 1720 820 703 617
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4512 4910 5184 5331 5742 4512
Size: 398 274 147 411 4849
Fragments arranged by size:
4849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5854
Fragments arranged by size:
5854 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2187 2220 1186
Size: 1001 33 5045
Fragments arranged by size:
5045 1001 33
BseYI C'CCAG_C
Cuts at: 2018 2121 2331 5282 5778 2018
Size: 103 210 2951 496 2319
Fragments arranged by size:
2951 2319 496 210 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3158 1163
Size: 573 1422 4084
Fragments arranged by size:
4084 1422 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3529 4290 5318 1540
Size: 275 186 48 1480 761 1028 2301
Fragments arranged by size:
2301 1480 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2454 4140 4225 5296 2454
Size: 1686 85 1071 3237
Fragments arranged by size:
3237 1686 1071 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2454 2573 2766 3127 3398 4140
Size: 399 229 435 119 193 361 271 742
Cuts at: 4140 4225 5296 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 361 271 229
193 119 85
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3434 844
Size: 2590 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2652 2992 4574 4748 65
Size: 1183 1404 340 1582 174 1396
Fragments arranged by size:
1582 1404 1396 1183 340 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3133 4723 4910 1700
Size: 110 1323 1590 187 2869
Fragments arranged by size:
2869 1590 1323 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2504 3145 794
Size: 1710 641 3728
Fragments arranged by size:
3728 1710 641
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 3244 1184
Size: 2060 4019
BstYI r'GATC_y
Cuts at: 1972 2381 3409 3776 4161 4178 5630 5716 5728
Size: 409 1028 367 385 17 1452 86 12
Cuts at: 5728 6074 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1028 409 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 3294 513
Size: 552 939 36 1254 3298
Fragments arranged by size:
3298 1254 939 552 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 2805 3595 4389 4409 2805
Size: 790 794 20 4475
Fragments arranged by size:
4475 794 790 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3242 5086 809
Size: 507 1926 1844 1802
Fragments arranged by size:
1926 1844 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2095 2395
Size: 54 1425 50 275 18 216 49 300
Cuts at: 2395 3184 3526 4415 6044 8
Size: 789 342 889 1629 43
Fragments arranged by size:
1629 1425 889 789 342 300 275 216
54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3526 1537
Size: 275 234 1480 4090
Fragments arranged by size:
4090 1480 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2589 4009 1353
Size: 707 529 1420 3423
Fragments arranged by size:
3423 1420 707 529
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2452 2452
Size: 6079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2836 3024 3129 4142 4739 5526 5794
Size: 393 1204 188 105 1013 597 787 268
Cuts at: 5794 1239
Size: 1524
Fragments arranged by size:
1524 1204 1013 787 597 393 268 188
105
EcoO109I rG'GnC_Cy
Cuts at: 3124 3235 3124
Size: 111 5968
EcoRI G'AATT_C
Cuts at: 2635 3388 3766 2635
Size: 753 378 4948
Fragments arranged by size:
4948 753 378
EcoRV GAT'ATC
Cuts at: 2269 2269
Size: 6079
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 2025 5226 1678
Size: 347 3201 2531
Fragments arranged by size:
3201 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3266 3298 4720 4752 4794
Size: 32 135 32 1694 32 1422 32 42
Cuts at: 4794 4826 1373
Size: 32 2626
Fragments arranged by size:
2626 1694 1422 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3423 3669 669
Size: 988 281 1485 246 3079
Fragments arranged by size:
3079 1485 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2338 2409 2886 5193 5377 1305
Size: 1033 71 477 2307 184 2007
Fragments arranged by size:
2307 2007 1033 477 184 71
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2397 3186 10
Size: 54 1425 343 565 789 2903
Fragments arranged by size:
2903 1425 789 565 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4037 4396 4555 518
Size: 648 2871 359 159 2042
Fragments arranged by size:
2871 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 3208 3495 4172 5320 5565 1606
Size: 436 1166 287 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 436 287 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3294 513
Size: 552 939 1290 3298
Fragments arranged by size:
3298 1290 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 2046 3526 2046
Size: 1480 4599
NspI r_CATG'y
Cuts at: 1132 1229 2498 2980 3094 3437 4982 1132
Size: 97 1269 482 114 343 1545 2229
Fragments arranged by size:
2229 1545 1269 482 343 114 97
PasI CC'CwG_GG
Cuts at: 1348 2573 1348
Size: 1225 4854
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 3120 3193 1108
Size: 1212 800 73 3994
Fragments arranged by size:
3994 1212 800 73
PfoI T'CCnGG_A
Cuts at: 3231 3231
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3235 3235
Size: 6079
PshAI GACnn'nnGTC
Cuts at: 1613 2886 1613
Size: 1273 4806
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 3123 3123
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2191 3431 830
Size: 1361 1240 3478
Fragments arranged by size:
3478 1361 1240
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 3208 3208
Size: 6079
SacI G_AGCT'C
Cuts at: 2454 2454
Size: 6079
SacII CC_GC'GG
Cuts at: 2043 2043
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 6079
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SexAI A'CCwGG_T
Cuts at: 2832 2832
Size: 6079
SfcI C'TryA_G
Cuts at: 826 1045 2187 2508 3427 4562 5243 5434 826
Size: 219 1142 321 919 1135 681 191 1471
Fragments arranged by size:
1471 1142 1135 919 681 321 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4183 5084 5346 820
Size: 197 926 2240 901 262 1553
Fragments arranged by size:
2240 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 2498 2980 3437 1132
Size: 1366 482 457 3774
Fragments arranged by size:
3774 1366 482 457
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 3192 3240 3294 3518 513
Size: 552 939 1188 48 54 224 3074
Fragments arranged by size:
3074 1188 939 552 224 54 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4325 6037 96
Size: 274 80 33 51 1443 2348 1712 138
Fragments arranged by size:
2348 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2772 2804 3153 3185 6039 6071 2772
Size: 32 349 32 2854 32 2780
Fragments arranged by size:
2854 2780 349 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 2629 3415 2629
Size: 786 5293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 2537 2317
Size: 220 5859
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 2135 4208 2135
Size: 2073 4006
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SfiI SfoI SrfI SwaI