Restriction map for
pFN21AE4090
(Circular) (Six-base) MAPSORT of: pFN21AE4090.seq Check: 5077 from: 1 to: 6073
pFN21AE4090 6073 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2268 3883 278
Size: 53 83 186 936 732 1615 2468
Fragments arranged by size:
2468 1615 936 732 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2994 3416 1937
Size: 1057 422 4594
Fragments arranged by size:
4594 1057 422
Acc65I G'GTAC_C
Cuts at: 3313 3394 3313
Size: 81 5992
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4136 5520 5788 1632
Size: 2504 1384 268 1917
Fragments arranged by size:
2504 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5876
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 3368 1166
Size: 2202 3871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6073
AlwNI CAG_nnn'CTG
Cuts at: 3816 5388 3816
Size: 1572 4501
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 3382 3608 3760 5869 1493
Size: 1889 226 152 2109 1697
Fragments arranged by size:
2109 1889 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4627 160
Size: 872 3595 1606
Fragments arranged by size:
3595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2351 2411 2489 3398 1870
Size: 73 408 60 78 909 4545
Fragments arranged by size:
4545 909 408 78 73 60
BamHI G'GATC_C
Cuts at: 3403 3770 3403
Size: 367 5706
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2184 2344 2777 3097
Size: 325 217 627 229 168 160 433 320
Cuts at: 3097 3313 3340 3394 4849 618
Size: 216 27 54 1455 1842
Fragments arranged by size:
1842 1455 627 433 325 320 229 217
216 168 160 54 27
BanII G_rGCy'C
Cuts at: 1391 2420 3131 3392 1391
Size: 1029 711 261 4072
Fragments arranged by size:
4072 1029 711 261
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2260 2473 2851 928
Size: 971 361 213 378 4150
Fragments arranged by size:
4150 971 378 361 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4684 136
Size: 107 122 71 631 605 3012 1525
Fragments arranged by size:
3012 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6068 6068
Size: 6073
BlpI GC'TnA_GC
Cuts at: 3490 3490
Size: 6073
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2780 3177 3343 4134 5290 1790
Size: 231 759 397 166 791 1156 2573
Fragments arranged by size:
2573 1156 791 759 397 231 166
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2783 3804 4762
Size: 416 53 83 399 24 1366 1021 958
Cuts at: 4762 442
Size: 1753
Fragments arranged by size:
1753 1366 1021 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2566 3296 4733 1239
Size: 1327 730 1437 2579
Fragments arranged by size:
2579 1437 1327 730
Bpu10I CC'TnA_GC
Cuts at: 3318 3318
Size: 6073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4198 5063 5361 4198
Size: 865 298 4910
Fragments arranged by size:
4910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4736 882
Size: 617 3237 2219
Fragments arranged by size:
3237 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2254 3864 493
Size: 711 1050 1610 2702
Fragments arranged by size:
2702 1610 1050 711
BsaBI GATnn'nnATC
Cuts at: 6067 6067
Size: 6073
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2202 2265 3880 4262
Size: 53 83 186 936 669 63 1615 382
Cuts at: 4262 4897 275
Size: 635 1451
Fragments arranged by size:
1615 1451 936 669 635 382 186 83
63 53
BsaWI w'CCGG_w
Cuts at: 2225 4506 4904 5178 5325 5736 2225
Size: 2281 398 274 147 411 2562
Fragments arranged by size:
2562 2281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2633 2663 1178
Size: 30 165 30 597 30 603 30 4588
Fragments arranged by size:
4588 603 597 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2936 1186
Size: 1750 4323
BseYI C'CCAG_C
Cuts at: 2095 2128 2192 2296 2938 3233 5276 5772 2095
Size: 33 64 104 642 295 2043 496 2396
Fragments arranged by size:
2396 2043 642 496 295 104 64 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5500
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2143 2182 2246 2518 3523 4284
Size: 275 186 142 39 64 272 1005 761
Cuts at: 4284 5312 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 1005 761 275 272 186 142
64 39
BsiHKAI G_wGCw'C
Cuts at: 2055 2211 2420 2439 4134 4219 5290 2055
Size: 156 209 19 1695 85 1071 2838
Fragments arranged by size:
2838 1695 1071 209 156 85 19
BsmI GAATG_Cn'
Cuts at: 3584 3677 3584
Size: 93 5980
BsmBI CGTCTCn'nnnn_
Cuts at: 2072 2473 2486 2636 2072
Size: 401 13 150 5509
Fragments arranged by size:
5509 401 150 13
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2055 2211 2420 2439 2780 3131
Size: 399 231 34 156 209 19 341 351
Cuts at: 3131 3177 3343 3392 4134 4219 5290 1391
Size: 46 166 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 341 231 209
166 156 85 49 46 34 19
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3428 844
Size: 2584 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2260 2567 3960 1394
Size: 429 221 216 307 1393 3507
Fragments arranged by size:
3507 1393 429 307 221 216
BsrDI GCAATG_nn'
Cuts at: 65 1248 3141 4568 4742 65
Size: 1183 1893 1427 174 1396
Fragments arranged by size:
1893 1427 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2181 2187 2245 2552 4717 4904 1700
Size: 110 371 6 58 307 2165 187 2869
Fragments arranged by size:
2869 2165 371 307 187 110 58 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 2087 2132 2159 2429 1925
Size: 162 45 27 270 5569
Fragments arranged by size:
5569 270 162 45 27
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6073
BstBI TT'CG_AA
Cuts at: 4877 4877
Size: 6073
BstEII G'GTnAC_C
Cuts at: 1184 3361 1184
Size: 2177 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 2228 2594 2824 2228
Size: 366 230 5477
Fragments arranged by size:
5477 366 230
BstYI r'GATC_y
Cuts at: 1972 3403 3770 4155 4172 5624 5710 5722 6068
Size: 1431 367 385 17 1452 86 12 346
Cuts at: 6068 1972
Size: 1977
Fragments arranged by size:
1977 1452 1431 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2137 513
Size: 552 939 8 125 4449
Fragments arranged by size:
4449 939 552 125 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5334
Fragments arranged by size:
5334 683 56
BtsI GCAGTG_nn'
Cuts at: 3589 4383 4403 3589
Size: 794 20 5259
Fragments arranged by size:
5259 794 20
ClaI AT'CG_AT
Cuts at: 1554 2739 1554
Size: 1185 4888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6038
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2140 2243
Size: 54 1425 50 275 18 231 79 103
Cuts at: 2243 2826 3520 4409 6038 8
Size: 583 694 889 1629 43
Fragments arranged by size:
1629 1425 889 694 583 275 231 103
79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2140 2243 3520 1537
Size: 275 328 103 1277 4090
Fragments arranged by size:
4090 1277 328 275 103
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 2966 3222 4003 1353
Size: 918 695 256 781 3423
Fragments arranged by size:
3423 918 781 695 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4660 5034 5180 387
Size: 1064 138 3071 374 146 1280
Fragments arranged by size:
3071 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2418 2418
Size: 6073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2566 3296 4136 4733 5520 5788 1239
Size: 393 934 730 840 597 787 268 1524
Fragments arranged by size:
1524 934 840 787 730 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2324 2276
Size: 48 6025
EcoO109I rG'GnC_Cy
Cuts at: 2125 2893 2125
Size: 768 5305
EcoRI G'AATT_C
Cuts at: 3382 3760 3382
Size: 378 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 6073
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 2359 2991 5220 1678
Size: 681 632 2229 2531
Fragments arranged by size:
2531 2229 681 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4714 4746 4788 4820 1373
Size: 32 135 32 3142 32 42 32 2626
Fragments arranged by size:
3142 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3010 3417 3663 669
Size: 988 281 1072 407 246 3079
Fragments arranged by size:
3079 1072 988 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3317 3398 3317
Size: 81 5992
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5187 5371 1305
Size: 3882 184 2007
Fragments arranged by size:
3882 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2828 10
Size: 54 1425 343 996 3255
Fragments arranged by size:
3255 1425 996 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 3368 4031 4390 4549 518
Size: 648 1803 399 663 359 159 2042
Fragments arranged by size:
2042 1803 663 648 399 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2139 2389 2572 3489 4166 5314 5559
Size: 408 125 250 183 917 677 1148 245
Cuts at: 5559 1606
Size: 2120
Fragments arranged by size:
2120 1148 917 677 408 250 245 183
125
NaeI GCC'GGC
Cuts at: 2247 2554 2247
Size: 307 5766
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4582
Fragments arranged by size:
4582 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NgoMIV G'CCGG_C
Cuts at: 2245 2552 2245
Size: 307 5766
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 2140 3520 2140
Size: 1380 4693
NspI r_CATG'y
Cuts at: 1132 1229 3259 3431 4976 1132
Size: 97 2030 172 1545 2229
Fragments arranged by size:
2229 2030 1545 172 97
PasI CC'CwG_GG
Cuts at: 1348 3347 1348
Size: 1999 4074
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6073
PfoI T'CCnGG_A
Cuts at: 2106 2271 2106
Size: 165 5908
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PmlI CAC'GTG
Cuts at: 2254 2254
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2125
Size: 6073
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PstI C_TGCA'G
Cuts at: 830 2545 3425 830
Size: 1715 880 3478
Fragments arranged by size:
3478 1715 880
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 2389 2389
Size: 6073
SacI G_AGCT'C
Cuts at: 2420 2420
Size: 6073
SacII CC_GC'GG
Cuts at: 2015 2140 2015
Size: 125 5948
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SanDI GG'GwC_CC
Cuts at: 2125 2125
Size: 6073
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SexAI A'CCwGG_T
Cuts at: 3357 3357
Size: 6073
SfcI C'TryA_G
Cuts at: 826 1045 2541 3075 3421 4556 5237 5428 826
Size: 219 1496 534 346 1135 681 191 1471
Fragments arranged by size:
1496 1471 1135 681 534 346 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 3400 1872
Size: 1528 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 4177 5078 5340 820
Size: 197 926 546 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 546 262 197
SnaBI TAC'GTA
Cuts at: 493 3864 493
Size: 3371 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3431 1132
Size: 2299 3774
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6073
StyI C'CwwG_G
Cuts at: 513 1065 2004 3512 513
Size: 552 939 1508 3074
Fragments arranged by size:
3074 1508 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3101 4100 4117 4270 4455 4900 325
Size: 1255 1521 999 17 153 185 445 1498
Fragments arranged by size:
1521 1498 1255 999 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4319 6031 96
Size: 274 80 33 51 1443 2342 1712 138
Fragments arranged by size:
2342 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2633 2665 2814 2846 6033 6065 2000
Size: 32 601 32 149 32 3187 32 2008
Fragments arranged by size:
3187 2008 601 149 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2935 3295 3333 2935
Size: 360 38 5675
Fragments arranged by size:
5675 360 38
XhoI C'TCGA_G
Cuts at: 1943 2489 1943
Size: 546 5527
XmaI C'CCGG_G
Cuts at: 1870 3398 1870
Size: 1528 4545
XmnI GAAnn'nnTTC
Cuts at: 4202 4202
Size: 6073
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2266 3881 276
Size: 53 83 186 936 732 1615 2468
Fragments arranged by size:
2468 1615 936 732 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI