Restriction map for
pFN21AE4088
(Circular) (Six-base) MAPSORT of: pFN21AE4088.seq Check: 8052 from: 1 to: 6061
pFN21AE4088 6061 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2723 4124 5508 5776 1632
Size: 1091 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1091 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2505 3804 5376 2505
Size: 1299 1572 3190
Fragments arranged by size:
3190 1572 1299
ApaI G_GGCC'C
Cuts at: 2826 2826
Size: 6061
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2008 3370 3596 3748 5857 1493
Size: 515 1362 226 152 2109 1697
Fragments arranged by size:
2109 1697 1362 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3386 1870
Size: 73 1443 4545
Fragments arranged by size:
4545 1443 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3382 4837 618
Size: 325 217 627 1595 1455 1842
Fragments arranged by size:
1842 1595 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2424 2814 2826 3004 3380 1391
Size: 1033 390 12 178 376 4072
Fragments arranged by size:
4072 1033 390 376 178 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2020 2180 928
Size: 971 121 160 4809
Fragments arranged by size:
4809 971 160 121
BbvCI CC'TCA_GC
Cuts at: 2403 2403
Size: 6061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 3280 1548
Size: 1732 4329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2100 2826 4122 5278 1790
Size: 251 59 726 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 726 251 59
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 3792 4750
Size: 416 53 83 399 24 655 1720 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1753 1720 958 655 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2723 4721 1239
Size: 1484 1998 2579
Fragments arranged by size:
2579 1998 1484
Bpu10I CC'TnA_GC
Cuts at: 2403 3003 2403
Size: 600 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2934 4186 5051 5349 2934
Size: 1252 865 298 3646
Fragments arranged by size:
3646 1252 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 3279 6055 3279
Size: 2776 3285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2367 4494 4892 5166 5313 5724 2367
Size: 2127 398 274 147 411 2704
Fragments arranged by size:
2704 2127 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2432 3140 3318 1186
Size: 1099 147 708 178 3929
Fragments arranged by size:
3929 1099 708 178 147
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2838 1163
Size: 573 1102 4386
Fragments arranged by size:
4386 1102 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2351 2367 2424 2814 4122 4207 5278 2162
Size: 189 16 57 390 1308 85 1071 2945
Fragments arranged by size:
2945 1308 1071 390 189 85 57 16
BsmI GAATG_Cn'
Cuts at: 2171 3572 3665 2171
Size: 1401 93 4567
Fragments arranged by size:
4567 1401 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2100 2162 2351 2367 2424 2814
Size: 399 251 59 62 189 16 57 390
Cuts at: 2814 2826 3004 3380 4122 4207 5278 1391
Size: 12 178 376 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 376 251 189
178 85 62 59 57 16 12
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 844
Size: 2572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2229 4556 4730 65
Size: 1183 981 2327 174 1396
Fragments arranged by size:
2327 1396 1183 981 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 4705 4892 1700
Size: 110 299 2596 187 2869
Fragments arranged by size:
2869 2596 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2150 794
Size: 1356 4705
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2747 2747
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 2698 3391 3758 4143 4160 5612 5698 5710
Size: 726 693 367 385 17 1452 86 12
Cuts at: 5710 6056 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 726 693 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3577 4371 4391 3166
Size: 411 794 20 4836
Fragments arranged by size:
4836 794 411 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026
Size: 54 1425 50 275 18 1678 889 1629
Cuts at: 6026 8
Size: 43
Fragments arranged by size:
1678 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 3092 3991 1353
Size: 1224 515 899 3423
Fragments arranged by size:
3423 1224 899 515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2422 2812 2422
Size: 390 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2723 4124 4721 5508 5776 1239
Size: 393 1091 1401 597 787 268 1524
Fragments arranged by size:
1524 1401 1091 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2822 2822
Size: 6061
EcoRI G'AATT_C
Cuts at: 2008 3370 3748 2008
Size: 1362 378 4321
Fragments arranged by size:
4321 1362 378
EcoRV GAT'ATC
Cuts at: 2965 2965
Size: 6061
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373
Size: 32 135 32 3130 32 42 32 2626
Fragments arranged by size:
3130 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2454 748
Size: 1706 4355
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3310 3660 3310
Size: 350 5711
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2917 5175 5359 1305
Size: 1071 541 2258 184 2007
Fragments arranged by size:
2258 2007 1071 541 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2766 4019 4378 4537 518
Size: 648 891 709 1253 359 159 2042
Fragments arranged by size:
2042 1253 891 709 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3477 4154 5302 5547 1606
Size: 1871 677 1148 245 2120
Fragments arranged by size:
2120 1871 1148 677 245
NaeI GCC'GGC
Cuts at: 2111 2111
Size: 6061
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5509
NdeI CA'TA_TG
Cuts at: 387 3042 387
Size: 2655 3406
NgoMIV G'CCGG_C
Cuts at: 2109 2109
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3419 4964 1132
Size: 97 2190 1545 2229
Fragments arranged by size:
2229 2190 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2394 2505 1108
Size: 1286 111 4664
Fragments arranged by size:
4664 1286 111
PfoI T'CCnGG_A
Cuts at: 3240 3240
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2822 2822
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2880 3173 3413 830
Size: 2050 293 240 3478
Fragments arranged by size:
3478 2050 293 240
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
SacI G_AGCT'C
Cuts at: 2424 2814 2424
Size: 390 5671
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SapI GCTCTTCn'nnn_
Cuts at: 2577 2577
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2876 3169 3349 3409 4544 5225 5416
Size: 219 1831 293 180 60 1135 681 191
Cuts at: 5416 826
Size: 1471
Fragments arranged by size:
1831 1471 1135 681 293 219 191 180
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2949 4165 5066 5328 820
Size: 197 926 1006 1216 901 262 1553
Fragments arranged by size:
1553 1216 1006 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3026 3985 5
Size: 47 2974 959 2081
Fragments arranged by size:
2974 2081 959 47
StuI AGG'CCT
Cuts at: 1517 3337 1517
Size: 1820 4241
StyI C'CwwG_G
Cuts at: 513 1065 3500 513
Size: 552 2435 3074
Fragments arranged by size:
3074 2435 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4307 6019 96
Size: 274 80 33 51 1443 2330 1712 138
Fragments arranged by size:
2330 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 2078 1316
Size: 762 5299
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2309 2985 4190 2309
Size: 676 1205 4180
Fragments arranged by size:
4180 1205 676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI