Restriction map for
pFN21AE4086
(Circular) (Six-base) MAPSORT of: pFN21AE4086.seq Check: 3284 from: 1 to: 6052
pFN21AE4086 6052 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3395 1937
Size: 1458 4594
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2839 4115 5499 5767 1632
Size: 1207 1276 1384 268 1917
Fragments arranged by size:
1917 1384 1276 1207 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 2685 4951 5928 1203
Size: 1482 2266 977 1327
Fragments arranged by size:
2266 1482 1327 977
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 2515 4781 2515
Size: 2266 3786
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6052
AlwNI CAG_nnn'CTG
Cuts at: 2115 2602 3795 5367 2115
Size: 487 1193 1572 2800
Fragments arranged by size:
2800 1572 1193 487
ApaI G_GGCC'C
Cuts at: 2343 2343
Size: 6052
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 3361 3587 3739 5848 1493
Size: 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 3377 1870
Size: 73 1434 4545
Fragments arranged by size:
4545 1434 73
BamHI G'GATC_C
Cuts at: 2962 3382 3749 2962
Size: 420 367 5265
Fragments arranged by size:
5265 420 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 4828 618
Size: 325 217 627 1586 1455 1842
Fragments arranged by size:
1842 1586 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2043 2307 2343 2811 3371 1391
Size: 652 264 36 468 560 4072
Fragments arranged by size:
4072 652 560 468 264 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2020 2217 928
Size: 971 121 197 4763
Fragments arranged by size:
4763 971 197 121
BbvCI CC'TCA_GC
Cuts at: 2400 2400
Size: 6052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4663 136
Size: 107 122 71 631 605 2991 1525
Fragments arranged by size:
2991 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6047 6047
Size: 6052
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2343 4113 5269 1790
Size: 310 243 1770 1156 2573
Fragments arranged by size:
2573 1770 1156 310 243
BmgBI CAC'GTC
Cuts at: 1318 2725 1318
Size: 1407 4645
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3037 3783 4741
Size: 416 53 83 399 24 1620 746 958
Cuts at: 4741 442
Size: 1753
Fragments arranged by size:
1753 1620 958 746 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2720 2850 4712 1239
Size: 1481 130 1862 2579
Fragments arranged by size:
2579 1862 1481 130
Bpu10I CC'TnA_GC
Cuts at: 2400 2400
Size: 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4177 5042 5340 4177
Size: 865 298 4889
Fragments arranged by size:
4889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4715 882
Size: 617 3216 2219
Fragments arranged by size:
3216 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3843 493
Size: 711 2639 2702
Fragments arranged by size:
2702 2639 711
BsaBI GATnn'nnATC
Cuts at: 2943 6046 2943
Size: 3103 2949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2077 4485 4883 5157 5304 5715 2077
Size: 2408 398 274 147 411 2414
Fragments arranged by size:
2414 2408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3163 3193 1178
Size: 30 165 30 1760 30 4037
Fragments arranged by size:
4037 1760 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 2591 2594 3040 3163 1186
Size: 1120 285 3 446 123 4075
Fragments arranged by size:
4075 1120 446 285 123 3
BseYI C'CCAG_C
Cuts at: 2790 5255 5751 2790
Size: 2465 496 3091
Fragments arranged by size:
3091 2465 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2188 1163
Size: 573 452 5027
Fragments arranged by size:
5027 573 452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2149 2286 2307 2364 2811 2872 2879 2956
Size: 106 137 21 57 447 61 7 77
Cuts at: 2956 4113 4198 5269 2043
Size: 1157 85 1071 2826
Fragments arranged by size:
2826 1157 1071 447 137 106 85 77
61 57 21 7
BsmI GAATG_Cn'
Cuts at: 2411 3005 3563 3656 2411
Size: 594 558 93 4807
Fragments arranged by size:
4807 594 558 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2100 2149 2286 2307 2343 2364
Size: 399 253 57 49 137 21 36 21
Cuts at: 2364 2811 2872 2879 2956 3371 4113 4198 5269
Size: 447 61 7 77 415 742 85 1071
Cuts at: 5269 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 447 415 399 253 137
85 77 61 57 49 36 21 21
7
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 3407 844
Size: 1184 1379 3489
Fragments arranged by size:
3489 1379 1184
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4696 4883 1700
Size: 110 2886 187 2869
Fragments arranged by size:
2886 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2688 3347 96
Size: 2592 659 2801
Fragments arranged by size:
2801 2592 659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 4112 5124 4112
Size: 1012 5040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2115 794
Size: 1321 4731
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 2080 1184
Size: 896 5156
BstXI CCAn_nnnn'nTGG
Cuts at: 2391 2391
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2074 2962 3248 3382 3749 4134 4151 5603
Size: 102 888 286 134 367 385 17 1452
Cuts at: 5603 5689 5701 6047 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 888 385 367 346 286 134
102 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2958 513
Size: 552 939 954 3607
Fragments arranged by size:
3607 954 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 3568 4362 4382 3568
Size: 794 20 5238
Fragments arranged by size:
5238 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2024 5059 809
Size: 507 708 3035 1802
Fragments arranged by size:
3035 1802 708 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3499 4388 6017
Size: 54 1425 50 275 18 1669 889 1629
Cuts at: 6017 8
Size: 43
Fragments arranged by size:
1669 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 2522 2613 3319 3982 1353
Size: 959 210 91 706 663 3423
Fragments arranged by size:
3423 959 706 663 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4639 5013 5159 387
Size: 1064 138 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2041 2305 2809 2041
Size: 264 504 5284
Fragments arranged by size:
5284 504 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2720 2839 2850 4115 4712 5499 5767
Size: 393 1088 119 11 1265 597 787 268
Cuts at: 5767 1239
Size: 1524
Fragments arranged by size:
1524 1265 1088 787 597 393 268 119
11
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 3145 2909
Size: 236 5816
EcoO109I rG'GnC_Cy
Cuts at: 2112 2340 2112
Size: 228 5824
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2323 3299 2209
Size: 114 976 4962
Fragments arranged by size:
4962 976 114
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 5199 1678
Size: 3521 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2265 2501 2533 3139
Size: 32 135 32 661 32 236 32 606
Cuts at: 3139 3171 3233 3265 4693 4725 4767 4799 1373
Size: 32 62 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 661 606 236 135 62 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2653 3078 5166 5350 1305
Size: 1348 425 2088 184 2007
Fragments arranged by size:
2088 2007 1348 425 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4230
Fragments arranged by size:
4230 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2882 2949 4010 4369 4528 518
Size: 648 891 825 67 1061 359 159 2042
Fragments arranged by size:
2042 1061 891 825 648 359 159 67
MspA1I CmG'CkG
Cuts at: 1606 3468 4145 5293 5538 1606
Size: 1862 677 1148 245 2120
Fragments arranged by size:
2120 1862 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4561
Fragments arranged by size:
4561 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 2689 2712 2719 2845 3410 4955 1132
Size: 97 1460 23 7 126 565 1545 2229
Fragments arranged by size:
2229 1545 1460 565 126 97 23 7
PasI CC'CwG_GG
Cuts at: 1348 2968 1348
Size: 1620 4432
PciI A'CATG_T
Cuts at: 2685 4951 2685
Size: 2266 3786
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2974 1108
Size: 1866 4186
PfoI T'CCnGG_A
Cuts at: 2736 3083 2736
Size: 347 5705
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 2423 3622 2423
Size: 1199 4853
PspOMI G'GGCC_C
Cuts at: 2339 2339
Size: 6052
PstI C_TGCA'G
Cuts at: 830 2598 3094 3404 830
Size: 1768 496 310 3478
Fragments arranged by size:
3478 1768 496 310
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
SacI G_AGCT'C
Cuts at: 2043 2307 2811 2043
Size: 264 504 5284
Fragments arranged by size:
5284 504 264
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SapI GCTCTTCn'nnn_
Cuts at: 2312 2613 2312
Size: 301 5751
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SfcI C'TryA_G
Cuts at: 826 1045 2594 3090 3160 3400 4535 5216 5407
Size: 219 1549 496 70 240 1135 681 191
Cuts at: 5407 826
Size: 1471
Fragments arranged by size:
1549 1471 1135 681 496 240 219 191
70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 3379 1872
Size: 1507 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4156 5057 5319 820
Size: 197 926 2213 901 262 1553
Fragments arranged by size:
2213 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 3208 3328 1517
Size: 1691 120 4241
Fragments arranged by size:
4241 1691 120
StyI C'CwwG_G
Cuts at: 513 1065 2004 3491 513
Size: 552 939 1487 3074
Fragments arranged by size:
3074 1487 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4079 4096 4249 4434 4879 325
Size: 1255 2499 17 153 185 445 1498
Fragments arranged by size:
2499 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 3347 4298
Size: 274 80 33 51 1443 711 659 951
Cuts at: 4298 6010 96
Size: 1712 138
Fragments arranged by size:
1712 1443 951 711 659 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3163 3195 6012 6044 3163
Size: 32 2817 32 3171
Fragments arranged by size:
3171 2817 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3061 3061
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 3377 1870
Size: 1507 4545
XmnI GAAnn'nnTTC
Cuts at: 2066 2886 4181 2066
Size: 820 1295 3937
Fragments arranged by size:
3937 1295 820
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI