Restriction map for
pFN21AE4079
(Circular) (Six-base) MAPSORT of: pFN21AE4079.seq Check: 511 from: 1 to: 5962
pFN21AE4079 5962 bp
With 182 enzymes: SgfI *
March 28, 2012 13:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2250 2526 3042 4025 5409 5677 1632
Size: 554 64 276 516 983 1384 268 1917
Fragments arranged by size:
1917 1384 983 554 516 276 268 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2381 4861 5838 1203
Size: 1178 2480 977 1327
Fragments arranged by size:
2480 1327 1178 977
AgeI A'CCGG_T
Cuts at: 2555 4793 2555
Size: 2238 3724
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2859 3271 3497 3649 5758 1493
Size: 1366 412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1366 412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2625 3179 4516 160
Size: 872 1593 554 1337 1606
Fragments arranged by size:
1606 1593 1337 872 554
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 3287 1870
Size: 73 1344 4545
Fragments arranged by size:
4545 1344 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2730 3281 1391
Size: 1339 551 4072
Fragments arranged by size:
4072 1339 551
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2169 2238 2829 928
Size: 971 270 69 591 4061
Fragments arranged by size:
4061 971 591 270 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2416 3854 5064 2416
Size: 1438 1210 3314
Fragments arranged by size:
3314 1438 1210
BclI T'GATC_A
Cuts at: 1548 2710 1548
Size: 1162 4800
BfrBI ATG'CAT
Cuts at: 3184 3184
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2593 2656 5957 2593
Size: 63 3301 2598
Fragments arranged by size:
3301 2598 63
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5179 1790
Size: 2233 1156 2573
Fragments arranged by size:
2573 2233 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2824 3693 4651
Size: 416 53 83 399 24 1407 869 958
Cuts at: 4651 442
Size: 1753
Fragments arranged by size:
1753 1407 958 869 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2277 3168 4622 1239
Size: 1038 891 1454 2579
Fragments arranged by size:
2579 1454 1038 891
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2419 2555 4395 4793 5067 5214 5625 2419
Size: 136 1840 398 274 147 411 2756
Fragments arranged by size:
2756 1840 411 398 274 147 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3141 3171 1178
Size: 30 165 30 1738 30 3969
Fragments arranged by size:
3969 1738 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5962
BseYI C'CCAG_C
Cuts at: 2431 5165 5661 2431
Size: 2734 496 2732
Fragments arranged by size:
2734 2732 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3016 3412 4173 5201 1540
Size: 275 186 1015 396 761 1028 2301
Fragments arranged by size:
2301 1028 1015 761 396 275 186
BsiHKAI G_wGCw'C
Cuts at: 2698 2730 4023 4108 5179 2698
Size: 32 1293 85 1071 3481
Fragments arranged by size:
3481 1293 1071 85 32
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2698 2730 3281 4023 4108 5179 1391
Size: 399 908 32 551 742 85 1071 2174
Fragments arranged by size:
2174 1071 908 742 551 399 85 32
BspEI T'CCGG_A
Cuts at: 2419 5625 2419
Size: 3206 2756
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3317 844
Size: 2473 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2555 3015 3213 4606 4793 1700
Size: 110 745 460 198 1393 187 2869
Fragments arranged by size:
2869 1393 745 460 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 3040 96
Size: 2944 3018
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 3115 4766 3115
Size: 1651 4311
BstEII G'GTnAC_C
Cuts at: 1184 2972 1184
Size: 1788 4174
BstXI CCAn_nnnn'nTGG
Cuts at: 2746 2746
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2593 2656 3292 3659 4044 4061 5513 5599
Size: 621 63 636 367 385 17 1452 86
Cuts at: 5599 5611 5957 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 636 621 385 367 346 86
63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2017 513
Size: 552 952 4458
Fragments arranged by size:
4458 952 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 2409 3478 4272 4292 2029
Size: 380 1069 794 20 3699
Fragments arranged by size:
3699 1069 794 380 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3013 3409 4298
Size: 54 1425 50 275 18 1183 396 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1183 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 3409 1537
Size: 275 1201 396 4090
Fragments arranged by size:
4090 1201 396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2449 2718 3080 3892 1353
Size: 1096 269 362 812 3423
Fragments arranged by size:
3423 1096 812 362 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2728 2728
Size: 5962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2250 2277 2526 3042 3168 4025
Size: 393 554 64 27 249 516 126 857
Cuts at: 4025 4622 5409 5677 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 857 787 597 554 516 393 268
249 126 64 27
EcoO109I rG'GnC_Cy
Cuts at: 2150 2941 2150
Size: 791 5171
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2381 2381
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 2567 2599 3141
Size: 32 135 32 596 32 367 32 542
Cuts at: 3141 3173 4603 4635 4677 4709 1373
Size: 32 1430 32 42 32 2626
Fragments arranged by size:
2626 1430 596 542 367 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 2566 3561 2566
Size: 995 4967
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 2786 3059 3920 4279 4438 518
Size: 648 1578 42 273 861 359 159 2042
Fragments arranged by size:
2042 1578 861 648 359 273 159 42
MspA1I CmG'CkG
Cuts at: 1606 2058 3378 4055 5203 5448 1606
Size: 452 1320 677 1148 245 2120
Fragments arranged by size:
2120 1320 1148 677 452 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NsiI A_TGCA'T
Cuts at: 3186 3186
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 3320 4865 1132
Size: 97 2091 1545 2229
Fragments arranged by size:
2229 2091 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2150 2150
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 3314 830
Size: 2484 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2058 2058
Size: 5962
SacI G_AGCT'C
Cuts at: 2730 2730
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SapI GCTCTTCn'nnn_
Cuts at: 2718 2718
Size: 5962
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2082 2881 3310 4445 5126 5317 826
Size: 219 1037 799 429 1135 681 191 1471
Fragments arranged by size:
1471 1135 1037 799 681 429 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4066 4967 5229 820
Size: 197 926 2123 901 262 1553
Fragments arranged by size:
2123 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 2852 3886 5
Size: 47 2800 1034 2081
Fragments arranged by size:
2800 2081 1034 47
StuI AGG'CCT
Cuts at: 1517 3007 1517
Size: 1490 4472
StyI C'CwwG_G
Cuts at: 513 1065 3401 513
Size: 552 2336 3074
Fragments arranged by size:
3074 2336 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3204 3989 4006 4159 4344 4789 325
Size: 1255 1624 785 17 153 185 445 1498
Fragments arranged by size:
1624 1498 1255 785 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2460 3040 4208
Size: 274 80 33 51 1443 483 580 1168
Cuts at: 4208 5920 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1168 580 483 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2401 2433 3092 3124 5922 5954 2401
Size: 32 659 32 2798 32 2409
Fragments arranged by size:
2798 2409 659 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2062
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI