Restriction map for
pFN21AE4076
(Circular) (Six-base) MAPSORT of: pFN21AE4076.seq Check: 7311 from: 1 to: 5911
pFN21AE4076 5911 bp
With 182 enzymes: SgfI *
March 28, 2012 13:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2585 3254 1937
Size: 648 669 4594
Fragments arranged by size:
4594 669 648
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2788 3189 3974 5358 5626 1632
Size: 1156 401 785 1384 268 1917
Fragments arranged by size:
1917 1384 1156 785 401 268
AfeI AGC'GCT
Cuts at: 2936 2936
Size: 5911
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 3654 5226 3654
Size: 1572 4339
ApaLI G'TGCA_C
Cuts at: 3968 5124 3968
Size: 1156 4755
ApoI r'AATT_y
Cuts at: 1493 2950 3220 3446 3598 5707 1493
Size: 1457 270 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 270 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2180 2360 2420 3178 3236 1870
Size: 73 228 9 180 60 758 58 4545
Fragments arranged by size:
4545 758 228 180 73 60 58 9
AvrII C'CTAG_G
Cuts at: 2752 2752
Size: 5911
BamHI G'GATC_C
Cuts at: 2756 3241 3608 2756
Size: 485 367 5059
Fragments arranged by size:
5059 485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2325 2405 2484 3232 4687
Size: 325 217 627 538 80 79 748 1455
Cuts at: 4687 618
Size: 1842
Fragments arranged by size:
1842 1455 748 627 538 325 217 80
79
BanII G_rGCy'C
Cuts at: 1391 3230 1391
Size: 1839 4072
BbeI G_GCGC'C
Cuts at: 2329 2409 2329
Size: 80 5831
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3009 3162 928
Size: 971 1110 153 3677
Fragments arranged by size:
3677 1110 971 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 4522 136
Size: 107 122 71 631 605 356 2494 1525
Fragments arranged by size:
2494 1525 631 605 356 122 107 71
BglII A'GATC_T
Cuts at: 2517 2847 5906 2517
Size: 330 3059 2522
Fragments arranged by size:
3059 2522 330
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2487 3972 5128 1790
Size: 697 1485 1156 2573
Fragments arranged by size:
2573 1485 1156 697
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2968 3642 4600
Size: 416 53 83 399 24 1551 674 958
Cuts at: 4600 442
Size: 1753
Fragments arranged by size:
1753 1551 958 674 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4571 1239
Size: 3332 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 2406 2897 4574 882
Size: 617 669 238 491 1677 2219
Fragments arranged by size:
2219 1677 669 617 491 238
BsaAI yAC'GTr
Cuts at: 493 1204 3702 493
Size: 711 2498 2702
Fragments arranged by size:
2702 2498 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2102 2326 2406
Size: 53 83 186 936 528 41 224 80
Cuts at: 2406 3718 4100 4735 275
Size: 1312 382 635 1451
Fragments arranged by size:
1451 1312 936 635 528 382 224 186
83 80 53 41
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2901 2931 1178
Size: 30 165 30 1498 30 4158
Fragments arranged by size:
4158 1498 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 1186
Size: 1594 4317
BseYI C'CCAG_C
Cuts at: 2480 5114 5610 2480
Size: 2634 496 2781
Fragments arranged by size:
2781 2634 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2251 2458 1163
Size: 573 515 207 4616
Fragments arranged by size:
4616 573 515 207
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2301 3361 4122 5150 1540
Size: 275 186 300 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 300 275 186
BsiHKAI G_wGCw'C
Cuts at: 3972 4057 5128 3972
Size: 85 1071 4755
Fragments arranged by size:
4755 1071 85
BsmI GAATG_Cn'
Cuts at: 3422 3515 3422
Size: 93 5818
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 2417
Size: 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2487 3230 3972 4057 5128 1391
Size: 399 697 743 742 85 1071 2174
Fragments arranged by size:
2174 1071 743 742 697 399 85
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 2902 3800 5530 2902
Size: 898 1730 3283
Fragments arranged by size:
3283 1730 898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 3266 844
Size: 1313 1109 3489
Fragments arranged by size:
3489 1313 1109
BsrBI CCG'CTC
Cuts at: 1394 1823 2055 2240 2398 3798 1394
Size: 429 232 185 158 1400 3507
Fragments arranged by size:
3507 1400 429 232 185 158
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2119 2279 2300 2375 4555 4742 1700
Size: 110 309 160 21 75 2180 187 2869
Fragments arranged by size:
2869 2180 309 187 160 110 75 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 2185 2476 1925
Size: 260 291 5360
Fragments arranged by size:
5360 291 260
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2520 794
Size: 1726 4185
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 2480
Size: 5911
BstYI r'GATC_y
Cuts at: 1972 2517 2756 2847 2875 3241 3608 3993 4010
Size: 545 239 91 28 366 367 385 17
Cuts at: 4010 5462 5548 5560 5906 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 545 385 367 366 346 239
91 86 28 17 12
BstZ17I GTA'TAC
Cuts at: 2586 2586
Size: 5911
BtgI C'CryG_G
Cuts at: 513 1065 2023 2133 2190 2225 513
Size: 552 958 110 57 35 4199
Fragments arranged by size:
4199 958 552 110 57 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 2823 3427 4221 4241 2464
Size: 359 604 794 20 4134
Fragments arranged by size:
4134 794 604 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2020 2281 2298
Size: 54 1425 50 275 18 190 261 17
Cuts at: 2298 3358 4247 5876 8
Size: 1060 889 1629 43
Fragments arranged by size:
1629 1425 1060 889 275 261 190 54
50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2298 3358 1537
Size: 275 486 1060 4090
Fragments arranged by size:
4090 1060 486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3841 1353
Size: 2488 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2247 4498 4872 5018 387
Size: 1064 138 474 184 2251 374 146 1280
Fragments arranged by size:
2251 1280 1064 474 374 184 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2788 3189 3974 4571 5358 5626 1239
Size: 393 1156 401 785 597 787 268 1524
Fragments arranged by size:
1524 1156 787 785 597 401 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 3097 2333
Size: 764 5147
EcoO109I rG'GnC_Cy
Cuts at: 3147 3147
Size: 5911
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 3048 2539
Size: 509 5402
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2329 2409 2938 5058 1678
Size: 651 80 529 2120 2531
Fragments arranged by size:
2531 2120 651 529 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3161 3193 4552 4584 4626
Size: 32 135 32 1589 32 1359 32 42
Cuts at: 4626 4658 1373
Size: 32 2626
Fragments arranged by size:
2626 1589 1359 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3255 3501 669
Size: 988 281 1152 165 246 3079
Fragments arranged by size:
3079 1152 988 281 246 165
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3090 3501 3090
Size: 411 5500
KasI G'GCGC_C
Cuts at: 2325 2405 2325
Size: 80 5831
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5025 5209 1305
Size: 3720 184 2007
Fragments arranged by size:
3720 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4089
Fragments arranged by size:
4089 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 2961 3869 4228 4387 518
Size: 648 1512 283 908 359 159 2042
Fragments arranged by size:
2042 1512 908 648 359 283 159
MspA1I CmG'CkG
Cuts at: 1606 2135 2158 2192 2227 2596 3327 4004 5152
Size: 529 23 34 35 369 731 677 1148
Cuts at: 5152 5397 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 731 677 529 369 245 35
34 23
NaeI GCC'GGC
Cuts at: 2121 2281 2302 2377 2121
Size: 160 21 75 5655
Fragments arranged by size:
5655 160 75 21
NarI GG'CG_CC
Cuts at: 2326 2406 2326
Size: 80 5831
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NgoMIV G'CCGG_C
Cuts at: 2119 2279 2300 2375 2119
Size: 160 21 75 5655
Fragments arranged by size:
5655 160 75 21
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2290 1108
Size: 1182 4729
PfoI T'CCnGG_A
Cuts at: 2331 2630 2331
Size: 299 5612
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PstI C_TGCA'G
Cuts at: 830 2274 2917 3263 830
Size: 1444 643 346 3478
Fragments arranged by size:
3478 1444 643 346
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2596 2596
Size: 5911
SacII CC_GC'GG
Cuts at: 2136 2193 2228 2136
Size: 57 35 5819
Fragments arranged by size:
5819 57 35
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2270 2913 3259 4394 5075 5266 826
Size: 219 1225 643 346 1135 681 191 1471
Fragments arranged by size:
1471 1225 1135 681 643 346 219 191
SfoI GGC'GCC
Cuts at: 2327 2407 2327
Size: 80 5831
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 2362 3238 1872
Size: 490 876 4545
Fragments arranged by size:
4545 876 490
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5911
StyI C'CwwG_G
Cuts at: 513 1065 2752 3098 3350 513
Size: 552 1687 346 252 3074
Fragments arranged by size:
3074 1687 552 346 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3093 3938 3955 4108 4293 4738 325
Size: 1255 1513 845 17 153 185 445 1498
Fragments arranged by size:
1513 1498 1255 845 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5869 96
Size: 274 80 33 51 1443 2180 1712 138
Fragments arranged by size:
2180 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2867 2899 5871 5903 2867
Size: 32 2972 32 2875
Fragments arranged by size:
2972 2875 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 2360 3236 1870
Size: 490 876 4545
Fragments arranged by size:
4545 876 490
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbvCI BfrBI Bpu10I
BsiWI Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI