Restriction map for
pFN21AE4074
(Circular) (Six-base) MAPSORT of: pFN21AE4074.seq Check: 2646 from: 1 to: 5905
pFN21AE4074 5905 bp
With 182 enzymes: SgfI *
March 28, 2012 13:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3006 3715 278
Size: 53 83 186 936 1470 709 2468
Fragments arranged by size:
2468 1470 936 709 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 3968 5352 5620 1632
Size: 440 1896 1384 268 1917
Fragments arranged by size:
1917 1896 1384 440 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2337 4804 5781 1203
Size: 1134 2467 977 1327
Fragments arranged by size:
2467 1327 1134 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2224 3103 3172 3648 5220 2224
Size: 879 69 476 1572 2909
Fragments arranged by size:
2909 1572 879 476 69
ApaI G_GGCC'C
Cuts at: 2715 2715
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 2643 3235 3602 2643
Size: 592 367 4946
Fragments arranged by size:
4946 592 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2127 3226 4681 618
Size: 325 217 627 340 1099 1455 1842
Fragments arranged by size:
1842 1455 1099 627 340 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2555 2715 2910 3192 3224 1391
Size: 670 494 160 195 282 32 4072
Fragments arranged by size:
4072 670 494 282 195 160 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 2235 1548
Size: 687 5218
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2399 2715 3966 5122 1790
Size: 609 316 1251 1156 2573
Fragments arranged by size:
2573 1251 1156 609 316
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 2635 3636
Size: 416 53 83 399 24 795 423 1001
Cuts at: 3636 4594 442
Size: 958 1753
Fragments arranged by size:
1753 1001 958 795 423 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4565 1239
Size: 3326 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2340 3696 493
Size: 711 1136 1356 2702
Fragments arranged by size:
2702 1356 1136 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3003 3712 4094 4729
Size: 53 83 186 936 1470 709 382 635
Cuts at: 4729 275
Size: 1451
Fragments arranged by size:
1470 1451 936 709 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2385 2529 4338 4736 5010 5157 5568 2385
Size: 144 1809 398 274 147 411 2722
Fragments arranged by size:
2722 1809 411 398 274 147 144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2432 3047 3050 1186
Size: 1246 615 3 4041
Fragments arranged by size:
4041 1246 615 3
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2000 1163
Size: 573 264 5068
Fragments arranged by size:
5068 573 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2555 2763 2910 3192 3966 4051 5122 2555
Size: 208 147 282 774 85 1071 3338
Fragments arranged by size:
3338 1071 774 282 208 147 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2399 2555 2715 2763 2910 3192
Size: 399 271 338 156 160 48 147 282
Cuts at: 3192 3224 3966 4051 5122 1391
Size: 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 282 271 160
156 147 85 48 32
BspEI T'CCGG_A
Cuts at: 2385 5568 2385
Size: 3183 2722
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2641 3792 1394
Size: 429 818 1151 3507
Fragments arranged by size:
3507 1151 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2250 4400 4574 65
Size: 1183 1002 2150 174 1396
Fragments arranged by size:
2150 1396 1183 1002 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 4549 4736 1700
Size: 110 518 2221 187 2869
Fragments arranged by size:
2869 2221 518 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 2800 2800
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2643 2808 3168 3235 3602 3987 4004 5456
Size: 671 165 360 67 367 385 17 1452
Cuts at: 5456 5542 5554 5900 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 671 385 367 360 346 165
86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2225 2225
Size: 5905
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2662 487
Size: 683 56 1436 3730
Fragments arranged by size:
3730 1436 683 56
BtsI GCAGTG_nn'
Cuts at: 2852 2969 3421 4215 4235 2852
Size: 117 452 794 20 4522
Fragments arranged by size:
4522 794 452 117 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2381 4912 809
Size: 507 1065 2531 1802
Fragments arranged by size:
2531 1802 1065 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2802 3352
Size: 54 1425 50 275 18 363 609 550
Cuts at: 3352 4241 5870 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 609 550 363 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3032 3835 1353
Size: 1679 803 3423
Fragments arranged by size:
3423 1679 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2133 4492 4866 5012 387
Size: 1064 138 544 2359 374 146 1280
Fragments arranged by size:
2359 1280 1064 544 374 146 138
EcoICRI GAG'CTC
Cuts at: 2553 2908 3190 2553
Size: 355 282 5268
Fragments arranged by size:
5268 355 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 3968 4565 5352 5620 1239
Size: 393 440 1896 597 787 268 1524
Fragments arranged by size:
1896 1524 787 597 440 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2711 2712 2711
Size: 1 5904
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373
Size: 32 135 32 2974 32 42 32 2626
Fragments arranged by size:
2974 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 2064 3504 2064
Size: 1440 4465
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 10
Size: 54 1425 343 363 3720
Fragments arranged by size:
3720 1425 363 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2546 3080 3863 4222 4381 518
Size: 648 1104 276 534 783 359 159 2042
Fragments arranged by size:
2042 1104 783 648 534 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2961 3321 3998 5146 5391 1606
Size: 1355 360 677 1148 245 2120
Fragments arranged by size:
2120 1355 1148 677 360 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2040 2448 2811 3090 2040
Size: 408 363 279 4855
Fragments arranged by size:
4855 408 363 279
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2711 2711
Size: 5905
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2204 2236 2204
Size: 32 5873
PstI C_TGCA'G
Cuts at: 830 2370 3141 3177 3257 830
Size: 1540 771 36 80 3478
Fragments arranged by size:
3478 1540 771 80 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
SacI G_AGCT'C
Cuts at: 2555 2910 3192 2555
Size: 355 282 5268
Fragments arranged by size:
5268 355 282
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2366 3137 3173 3253 4388 5069 5260
Size: 219 1321 771 36 80 1135 681 191
Cuts at: 5260 826
Size: 1471
Fragments arranged by size:
1471 1321 1135 771 681 219 191 80
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2163 2256 3183 3344 513
Size: 552 1098 93 927 161 3074
Fragments arranged by size:
3074 1098 927 552 161 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 3932 3949 4102 4287 4732 325
Size: 1255 678 1674 17 153 185 445 1498
Fragments arranged by size:
1674 1498 1255 678 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2518 2550 5865 5897 2518
Size: 32 3315 32 2526
Fragments arranged by size:
3315 2526 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2263
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 2475 4034 2475
Size: 1559 4346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3004 3713 276
Size: 53 83 186 936 1470 709 2468
Fragments arranged by size:
2468 1470 936 709 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI