Restriction map for
pFN21AE4073
(Circular) (Six-base) MAPSORT of: pFN21AE4073.seq Check: 2957 from: 1 to: 5851
pFN21AE4073 5851 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2465 2601 2465
Size: 136 5715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2115 3661 278
Size: 53 83 186 936 579 1546 2468
Fragments arranged by size:
2468 1546 936 579 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2380 2548 3914 5298 5566 1632
Size: 748 168 1366 1384 268 1917
Fragments arranged by size:
1917 1384 1366 748 268 168
AfeI AGC'GCT
Cuts at: 2393 2393
Size: 5851
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 2844 4750 5727 1203
Size: 1641 1906 977 1327
Fragments arranged by size:
1906 1641 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 3051 3594 5166 3051
Size: 543 1572 3736
Fragments arranged by size:
3736 1572 543
ApaI G_GGCC'C
Cuts at: 2673 2811 2908 2673
Size: 138 97 5616
Fragments arranged by size:
5616 138 97
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3160 3386 3538 5647 1493
Size: 1667 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 226 152
AscI GG'CGCG_CC
Cuts at: 2745 2821 3030 2745
Size: 76 209 5566
Fragments arranged by size:
5566 209 76
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 3125 3176 1870
Size: 73 981 201 51 4545
Fragments arranged by size:
4545 981 201 73 51
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 2990 3120 3172 4627
Size: 325 217 627 466 737 130 52 1455
Cuts at: 4627 618
Size: 1842
Fragments arranged by size:
1842 1455 737 627 466 325 217 130
52
BanII G_rGCy'C
Cuts at: 1391 2324 2408 2673 2811 2889 2908 2925 3170
Size: 933 84 265 138 78 19 17 245
Cuts at: 3170 1391
Size: 4072
Fragments arranged by size:
4072 933 265 245 138 84 78 19
17
BbeI G_GCGC'C
Cuts at: 2994 2994
Size: 5851
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4042 4076 1903
Size: 34 2105 34 3678
Fragments arranged by size:
3678 2105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2179 4462 136
Size: 107 122 71 631 605 507 2283 1525
Fragments arranged by size:
2283 1525 631 605 507 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 3268 3268
Size: 5851
Bme1580I G_kGCm'C
Cuts at: 1790 2546 2673 2811 2877 2908 3125 3912 5068
Size: 756 127 138 66 31 217 787 1156
Cuts at: 5068 1790
Size: 2573
Fragments arranged by size:
2573 1156 787 756 217 138 127 66
31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 2143 3582
Size: 416 53 83 399 24 658 68 1439
Cuts at: 3582 4540 442
Size: 958 1753
Fragments arranged by size:
1753 1439 958 658 416 399 83 68
53 24
BmtI G_CTAG'C
Cuts at: 1056 2652 1056
Size: 1596 4255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2422 2873 4511 1239
Size: 1183 451 1638 2579
Fragments arranged by size:
2579 1638 1183 451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 3976 4841 5139 2513
Size: 1463 865 298 3225
Fragments arranged by size:
3225 1463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2034 4514 882
Size: 617 535 2480 2219
Fragments arranged by size:
2480 2219 617 535
BsaAI yAC'GTr
Cuts at: 493 1204 2845 3642 493
Size: 711 1641 797 2702
Fragments arranged by size:
2702 1641 797 711
BsaBI GATnn'nnATC
Cuts at: 2705 5845 2705
Size: 3140 2711
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2991 3658 4040
Size: 53 83 186 936 579 879 667 382
Cuts at: 4040 4675 275
Size: 635 1451
Fragments arranged by size:
1451 936 879 667 635 579 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3000 4284 4682 4956 5103 5514 3000
Size: 1284 398 274 147 411 3337
Fragments arranged by size:
3337 1284 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5626
Fragments arranged by size:
5626 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2957 3098 3101 1186
Size: 1771 141 3 3936
Fragments arranged by size:
3936 1771 141 3
BseYI C'CCAG_C
Cuts at: 2117 2161 2907 5054 5550 2117
Size: 44 746 2147 496 2418
Fragments arranged by size:
2418 2147 746 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2585 3005 1163
Size: 573 618 231 420 4009
Fragments arranged by size:
4009 618 573 420 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2183 2189 2473 2557 2769 2816
Size: 275 186 182 6 284 84 212 47
Cuts at: 2816 3301 4062 5090 1540
Size: 485 761 1028 2301
Fragments arranged by size:
2301 1028 761 485 284 275 212 186
182 84 47 6
BsiHKAI G_wGCw'C
Cuts at: 2324 2408 3912 3997 5068 2324
Size: 84 1504 85 1071 3107
Fragments arranged by size:
3107 1504 1071 85 84
BsiWI C'GTAC_G
Cuts at: 2841 2841
Size: 5851
BsmI GAATG_Cn'
Cuts at: 3362 3455 3362
Size: 93 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2324 2408 2546 2673 2811 2877 2889
Size: 399 534 84 138 127 138 66 12
Cuts at: 2889 2908 2925 3125 3170 3912 3997 5068 1391
Size: 19 17 200 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 534 399 200 138 138
127 85 84 66 45 19 17 12
BspEI T'CCGG_A
Cuts at: 3000 5514 3000
Size: 2514 3337
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2465 2601 3206 844
Size: 1621 136 605 3489
Fragments arranged by size:
3489 1621 605 136
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 3738 1394
Size: 429 455 1460 3507
Fragments arranged by size:
3507 1460 455 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 2270 2340 2508 2947 4495 4682
Size: 110 355 105 70 168 439 1548 187
Cuts at: 4682 1700
Size: 2869
Fragments arranged by size:
2869 1548 439 355 187 168 110 105
70
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 2745 2821 3030 1925
Size: 820 76 209 4746
Fragments arranged by size:
4746 820 209 76
BssSI C'ACGA_G
Cuts at: 2072 3911 4923 2072
Size: 1839 1012 3000
Fragments arranged by size:
3000 1839 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2332 3051 794
Size: 1538 719 3594
Fragments arranged by size:
3594 1538 719
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 2713 2641
Size: 72 5779
BstYI r'GATC_y
Cuts at: 1972 3015 3181 3548 3933 3950 5402 5488 5500
Size: 1043 166 367 385 17 1452 86 12
Cuts at: 5500 5846 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1043 385 367 346 166 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2183 2557 2712 2763 2769 2778
Size: 552 939 179 374 155 51 6 9
Cuts at: 2778 2802 3108 3133 513
Size: 24 306 25 3231
Fragments arranged by size:
3231 939 552 374 306 179 155 51
25 24 9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2733 487
Size: 683 56 1507 3605
Fragments arranged by size:
3605 1507 683 56
BtsI GCAGTG_nn'
Cuts at: 3367 4161 4181 3367
Size: 794 20 5037
Fragments arranged by size:
5037 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2197 2232 529
Size: 35 1633 35 4148
Fragments arranged by size:
4148 1633 35 35
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DraIII CAC_nnn'GTG
Cuts at: 2332 3025 2332
Size: 693 5158
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2180 2186
Size: 54 1425 50 275 18 234 116 6
Cuts at: 2186 2470 2499 2554 2631 2766 3298 4187 5816
Size: 284 29 55 77 135 532 889 1629
Cuts at: 5816 8
Size: 43
Fragments arranged by size:
1629 1425 889 532 284 275 234 135
116 77 55 54 50 43 29 18
6
EagI C'GGCC_G
Cuts at: 1537 1812 2180 2186 2470 2554 2766 3298 1537
Size: 275 368 6 284 84 212 532 4090
Fragments arranged by size:
4090 532 368 284 275 212 84 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 3781 1353
Size: 1317 1111 3423
Fragments arranged by size:
3423 1317 1111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2705 4438 4812 4958 387
Size: 1064 138 1116 1733 374 146 1280
Fragments arranged by size:
1733 1280 1116 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2322 2406 2322
Size: 84 5767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2380 2422 2548 2873 3914 4511 5298
Size: 393 748 42 126 325 1041 597 787
Cuts at: 5298 5566 1239
Size: 268 1524
Fragments arranged by size:
1524 1041 787 748 597 393 325 268
126 42
EcoO109I rG'GnC_Cy
Cuts at: 2618 2669 2807 2808 2618
Size: 51 138 1 5661
Fragments arranged by size:
5661 138 51 1
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 2395 2515 2994 4998 1678
Size: 717 120 479 2004 2531
Fragments arranged by size:
2531 2004 717 479 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2085 4492 4524 4566
Size: 32 135 32 481 32 2407 32 42
Cuts at: 4566 4598 1373
Size: 32 2626
Fragments arranged by size:
2626 2407 481 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KasI G'GCGC_C
Cuts at: 2990 2990
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 4965 5149 1305
Size: 1128 2532 184 2007
Fragments arranged by size:
2532 2007 1128 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 2501 10
Size: 54 1425 343 234 435 3360
Fragments arranged by size:
3360 1425 435 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 2735 2738 2960 3809 4168 4327
Size: 648 1545 24 3 222 849 359 159
Cuts at: 4327 518
Size: 2042
Fragments arranged by size:
2042 1545 849 648 359 222 159 24
3
MspA1I CmG'CkG
Cuts at: 1606 2041 2121 2185 2400 2523 2559 2765 2771
Size: 435 80 64 215 123 36 206 6
Cuts at: 2771 2804 2902 3051 3110 3135 3267 3944 5092
Size: 33 98 149 59 25 132 677 1148
Cuts at: 5092 5337 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 435 245 215 206 149
132 123 98 80 64 59 36 33
25 6
NaeI GCC'GGC
Cuts at: 2167 2949 2167
Size: 782 5069
NarI GG'CG_CC
Cuts at: 2991 2991
Size: 5851
NcoI C'CATG_G
Cuts at: 513 1065 2004 2778 513
Size: 552 939 774 3586
Fragments arranged by size:
3586 939 774 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NgoMIV G'CCGG_C
Cuts at: 2165 2947 2165
Size: 782 5069
NheI G'CTAG_C
Cuts at: 1052 2648 1052
Size: 1596 4255
NotI GC'GGCC_GC
Cuts at: 2186 2470 2554 2766 3298 2186
Size: 284 84 212 532 4739
Fragments arranged by size:
4739 532 284 212 84
NspI r_CATG'y
Cuts at: 1132 1229 3209 4754 1132
Size: 97 1980 1545 2229
Fragments arranged by size:
2229 1980 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5851
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2062 3025 1108
Size: 954 963 3934
Fragments arranged by size:
3934 963 954
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2669 2807 2904 2669
Size: 138 97 5616
Fragments arranged by size:
5616 138 97
PstI C_TGCA'G
Cuts at: 830 3047 3203 830
Size: 2217 156 3478
Fragments arranged by size:
3478 2217 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
PvuII CAG'CTG
Cuts at: 2121 2400 3051 2121
Size: 279 651 4921
Fragments arranged by size:
4921 651 279
SacI G_AGCT'C
Cuts at: 2324 2408 2324
Size: 84 5767
SacII CC_GC'GG
Cuts at: 2186 2560 2766 2772 2805 3111 3136 2186
Size: 374 206 6 33 306 25 4901
Fragments arranged by size:
4901 374 306 206 33 25 6
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SexAI A'CCwGG_T
Cuts at: 2328 2328
Size: 5851
SfcI C'TryA_G
Cuts at: 826 1045 3043 3199 4334 5015 5206 826
Size: 219 1998 156 1135 681 191 1471
Fragments arranged by size:
1998 1471 1135 681 219 191 156
SfoI GGC'GCC
Cuts at: 2992 2992
Size: 5851
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 2270 2340 2508 1700
Size: 570 70 168 5043
Fragments arranged by size:
5043 570 168 70
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 3955 4856 5118 820
Size: 197 926 585 1427 901 262 1553
Fragments arranged by size:
1553 1427 926 901 585 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2004 2634 2664 2778 3290 513
Size: 552 939 630 30 114 512 3074
Fragments arranged by size:
3074 939 630 552 512 114 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3028 3878 3895 4048 4233 4678 325
Size: 1255 1448 850 17 153 185 445 1498
Fragments arranged by size:
1498 1448 1255 850 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4097 5809 96
Size: 274 80 33 51 1443 2120 1712 138
Fragments arranged by size:
2120 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 1316
Size: 795 5056
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 2867 3980 2867
Size: 1113 4738
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2113 3659 276
Size: 53 83 186 936 579 1546 2468
Fragments arranged by size:
2468 1546 936 579 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SanDI SapI SfiI SrfI SwaI XcmI