Restriction map for
pFN21AE4070
(Circular) (Six-base) MAPSORT of: pFN21AE4070.seq Check: 1674 from: 1 to: 5824
pFN21AE4070 5824 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2643 2843 3887 5271 5539 1632
Size: 749 262 200 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 749 268 262 200
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2534 3111 4723 5700 1203
Size: 1331 577 1612 977 1327
Fragments arranged by size:
1612 1331 1327 977 577
AgeI A'CCGG_T
Cuts at: 2114 2898 4655 2114
Size: 784 1757 3283
Fragments arranged by size:
3283 1757 784
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2181 2181
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3107 3881 5037 3107
Size: 774 1156 3894
Fragments arranged by size:
3894 1156 774
ApoI r'AATT_y
Cuts at: 1493 2500 2829 2842 3133 3359 3511 5620 1493
Size: 1007 329 13 291 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 329 291 226 152 13
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 3149 1870
Size: 73 795 411 4545
Fragments arranged by size:
4545 795 411 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 3145 4600 618
Size: 325 217 627 231 1127 1455 1842
Fragments arranged by size:
1842 1455 1127 627 325 231 217
BanII G_rGCy'C
Cuts at: 1391 2181 2745 3143 1391
Size: 790 564 398 4072
Fragments arranged by size:
4072 790 564 398
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2436 2571 2973 928
Size: 971 537 135 402 3779
Fragments arranged by size:
3779 971 537 402 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2621 5819 2621
Size: 3198 2626
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2181 3033 3111 3885 5041 1790
Size: 391 852 78 774 1156 2573
Fragments arranged by size:
2573 1156 852 774 391 78
BmgBI CAC'GTC
Cuts at: 1318 2025 1318
Size: 707 5117
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3555 4513 442
Size: 416 53 83 399 24 2138 958 1753
Fragments arranged by size:
2138 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2646 3041 4484 1239
Size: 855 552 395 1443 2579
Fragments arranged by size:
2579 1443 855 552 395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2601 4487 882
Size: 617 1102 1886 2219
Fragments arranged by size:
2219 1886 1102 617
BsaAI yAC'GTr
Cuts at: 493 1204 3112 3615 493
Size: 711 1908 503 2702
Fragments arranged by size:
2702 1908 711 503
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2114 2898 4257 4655 4929 5076 5487 2114
Size: 784 1359 398 274 147 411 2451
Fragments arranged by size:
2451 1359 784 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5599
Fragments arranged by size:
5599 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5824
BseYI C'CCAG_C
Cuts at: 5027 5523 5027
Size: 496 5328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3028 1163
Size: 573 1292 3959
Fragments arranged by size:
3959 1292 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3111 3885 3970 5041 3111
Size: 774 85 1071 3894
Fragments arranged by size:
3894 1071 774 85
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2745 3033 3111 3143 3885 3970
Size: 399 391 564 288 78 32 742 85
Cuts at: 3970 5041 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 564 399 391 288 85
78 32
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2382 3050 3179 844
Size: 1538 668 129 3489
Fragments arranged by size:
3489 1538 668 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2944 3711 1394
Size: 429 1121 767 3507
Fragments arranged by size:
3507 1121 767 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 2632 2898 3084 4468 4655 1700
Size: 110 304 518 266 186 1384 187 2869
Fragments arranged by size:
2869 1384 518 304 266 187 186 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3051 794
Size: 2257 3567
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 3037 1184
Size: 1853 3971
BstYI r'GATC_y
Cuts at: 1972 2440 2621 3154 3521 3906 3923 5375 5461
Size: 468 181 533 367 385 17 1452 86
Cuts at: 5461 5473 5819 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 533 468 385 367 346 181
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 3340 4134 4154 3340
Size: 794 20 5010
Fragments arranged by size:
5010 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 3051 3051
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3271 4160 5789
Size: 54 1425 50 275 18 1441 889 1629
Cuts at: 5789 8
Size: 43
Fragments arranged by size:
1629 1441 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3754 1353
Size: 2401 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2381 2643 2646 2843 3041 3887
Size: 393 462 287 262 3 197 198 846
Cuts at: 3887 4484 5271 5539 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 846 787 597 462 393 287 268
262 198 197 3
EcoO109I rG'GnC_Cy
Cuts at: 2177 2371 2511 2177
Size: 194 140 5490
Fragments arranged by size:
5490 194 140
EcoRI G'AATT_C
Cuts at: 2500 3133 3511 2500
Size: 633 378 4813
Fragments arranged by size:
4813 633 378
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2344 4971 1678
Size: 666 2627 2531
Fragments arranged by size:
2627 2531 666
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2600 2632 3058
Size: 32 135 32 677 32 319 32 426
Cuts at: 3058 3090 4465 4497 4539 4571 1373
Size: 32 1375 32 42 32 2626
Fragments arranged by size:
2626 1375 677 426 319 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2851 3168 3414 669
Size: 988 281 913 317 246 3079
Fragments arranged by size:
3079 988 913 317 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4002
Fragments arranged by size:
4002 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2990 3782 4141 4300 518
Size: 648 861 963 792 359 159 2042
Fragments arranged by size:
2042 963 861 792 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2821 2953 3240 3917 5065 5310 1606
Size: 1215 132 287 677 1148 245 2120
Fragments arranged by size:
2120 1215 1148 677 287 245 132
NaeI GCC'GGC
Cuts at: 2634 3086 2634
Size: 452 5372
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5272
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2632 3084 2632
Size: 452 5372
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2237 3182 4727 1132
Size: 97 1008 945 1545 2229
Fragments arranged by size:
2229 1545 1008 945 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PmlI CAC'GTG
Cuts at: 3112 3112
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2511 2371
Size: 140 5684
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2177 2177
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2952 2958 3176 830
Size: 2122 6 218 3478
Fragments arranged by size:
3478 2122 218 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2821 2953 2821
Size: 132 5692
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 2448 4072 5784 2448
Size: 1624 1712 2488
Fragments arranged by size:
2488 1712 1624
SfcI C'TryA_G
Cuts at: 826 1045 2948 2954 3172 4307 4988 5179 826
Size: 219 1903 6 218 1135 681 191 1471
Fragments arranged by size:
1903 1471 1135 681 219 218 191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2740 3151 1872
Size: 868 411 4545
Fragments arranged by size:
4545 868 411
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2748 3263 513
Size: 552 1683 515 3074
Fragments arranged by size:
3074 1683 552 515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2446 4070 5782
Size: 274 80 33 51 1443 469 1624 1712
Cuts at: 5782 96
Size: 138
Fragments arranged by size:
1712 1624 1443 469 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2002 2034 2796 2828 5784 5816 2002
Size: 32 762 32 2956 32 2010
Fragments arranged by size:
2956 2010 762 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3011 1316
Size: 1695 4129
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2933 2933
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2738 3149 1870
Size: 868 411 4545
Fragments arranged by size:
4545 868 411
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI