Restriction map for
pFN21AE4066
(Circular) (Six-base) MAPSORT of: pFN21AE4066.seq Check: 4472 from: 1 to: 5743
pFN21AE4066 5743 bp
With 182 enzymes: SgfI *
March 28, 2012 13:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2739 3086 1937
Size: 802 347 4594
Fragments arranged by size:
4594 802 347
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2616 3806 5190 5458 1632
Size: 431 553 1190 1384 268 1917
Fragments arranged by size:
1917 1384 1190 553 431 268
AfeI AGC'GCT
Cuts at: 2756 2939 2756
Size: 183 5560
AflII C'TTAA_G
Cuts at: 820 1017 2371 820
Size: 197 1354 4192
Fragments arranged by size:
4192 1354 197
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 2287 4472 2287
Size: 2185 3558
AleI CACnn'nnGTG
Cuts at: 1166 2515 1166
Size: 1349 4394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2488 2656 3486 5058 2488
Size: 168 830 1572 3173
Fragments arranged by size:
3173 1572 830 168
ApaI G_GGCC'C
Cuts at: 2200 2354 2876 2200
Size: 154 522 5067
Fragments arranged by size:
5067 522 154
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2094 2148 2199 2269 3068 1870
Size: 73 133 18 54 51 70 799 4545
Fragments arranged by size:
4545 799 133 73 70 54 51 18
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 3064 4519 618
Size: 325 217 627 338 939 1455 1842
Fragments arranged by size:
1842 1455 939 627 338 325 217
BanII G_rGCy'C
Cuts at: 1391 2200 2354 2370 2450 2466 2579 2876 3062
Size: 809 154 16 80 16 113 297 186
Cuts at: 3062 1391
Size: 4072
Fragments arranged by size:
4072 809 297 186 154 113 80 16
16
BbeI G_GCGC'C
Cuts at: 2129 2129
Size: 5743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4772
BbvCI CC'TCA_GC
Cuts at: 2934 2934
Size: 5743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3934 3968 1903
Size: 34 50 34 1913 34 3678
Fragments arranged by size:
3678 1913 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2211 4354
Size: 107 122 71 631 605 437 102 2143
Cuts at: 4354 136
Size: 1525
Fragments arranged by size:
2143 1525 631 605 437 122 107 102
71
BglII A'GATC_T
Cuts at: 2667 5738 2667
Size: 3071 2672
BlpI GC'TnA_GC
Cuts at: 2294 3160 2294
Size: 866 4877
Bme1580I G_kGCm'C
Cuts at: 1790 2200 2354 2876 3804 4960 1790
Size: 410 154 522 928 1156 2573
Fragments arranged by size:
2573 1156 928 522 410 154
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2473 4403 1239
Size: 1234 1930 2579
Fragments arranged by size:
2579 1930 1234
Bpu10I CC'TnA_GC
Cuts at: 2934 2934
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1994 3868 4733 5031 1994
Size: 1874 865 298 2706
Fragments arranged by size:
2706 1874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 2283 3550 3932
Size: 53 83 186 936 593 157 1267 382
Cuts at: 3932 4567 275
Size: 635 1451
Fragments arranged by size:
1451 1267 936 635 593 382 186 157
83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5518
Fragments arranged by size:
5518 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2186 2722 2832 1186
Size: 997 3 536 110 4097
Fragments arranged by size:
4097 997 536 110 3
BseYI C'CCAG_C
Cuts at: 2121 2902 2910 4946 5442 2121
Size: 781 8 2036 496 2422
Fragments arranged by size:
2422 2036 781 496 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 1163
Size: 573 653 4517
Fragments arranged by size:
4517 653 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2466 3804 3889 4960 2370
Size: 96 1338 85 1071 3153
Fragments arranged by size:
3153 1338 1071 96 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2200 2354 2370 2450 2466 2579 2876
Size: 399 410 154 16 80 16 113 297
Cuts at: 2876 3062 3804 3889 4960 1391
Size: 186 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 297 186 154
113 85 80 16 16
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3024 3632 5362 3024
Size: 608 1730 3405
Fragments arranged by size:
3405 1730 608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2210 4387 4574 1700
Size: 110 400 2177 187 2869
Fragments arranged by size:
2869 2177 400 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 2274 1925
Size: 349 5394
BssSI C'ACGA_G
Cuts at: 2890 3803 4815 2890
Size: 913 1012 3818
Fragments arranged by size:
3818 1012 913
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5743
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2517 2572 2517
Size: 55 5688
BstYI r'GATC_y
Cuts at: 1972 2337 2653 2667 3073 3440 3825 3842 5294
Size: 365 316 14 406 367 385 17 1452
Cuts at: 5294 5380 5392 5738 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 406 385 367 365 346 316
86 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2192 2225 513
Size: 552 1127 33 4031
Fragments arranged by size:
4031 1127 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 2522 2772 2783 3259 4053 4073 2510
Size: 12 250 11 476 794 20 4180
Fragments arranged by size:
4180 794 476 250 20 12 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2978 3010
Size: 54 1425 50 275 18 378 770 32
Cuts at: 3010 3190 4079 5708 8
Size: 180 889 1629 43
Fragments arranged by size:
1629 1425 889 770 378 275 180 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2588 3673 1353
Size: 1235 1085 3423
Fragments arranged by size:
3423 1235 1085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2368 2464 2368
Size: 96 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2473 2616 3806 4403 5190 5458
Size: 393 431 410 143 1190 597 787 268
Cuts at: 5458 1239
Size: 1524
Fragments arranged by size:
1524 1190 787 597 431 410 393 268
143
EcoO109I rG'GnC_Cy
Cuts at: 2203 2350 2485 2785 2852 2873 2203
Size: 147 135 300 67 21 5073
Fragments arranged by size:
5073 300 147 135 67 21
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2129 2758 2941 4890 1678
Size: 451 629 183 1949 2531
Fragments arranged by size:
2531 1949 629 451 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 3006 3038 4384
Size: 32 135 32 701 32 701 32 1346
Cuts at: 4384 4416 4458 4490 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1346 701 701 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2125 2125
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 4857 5041 1305
Size: 861 2691 184 2007
Fragments arranged by size:
2691 2007 861 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2515 3701 4060 4219 518
Size: 648 1349 1186 359 159 2042
Fragments arranged by size:
2042 1349 1186 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2194 2227 2260 2313 2385 2430 2906 2974
Size: 588 33 33 53 72 45 476 68
Cuts at: 2974 3159 3836 4984 5229 1606
Size: 185 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 588 476 245 185 72
68 53 45 33 33
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 5743
NarI GG'CG_CC
Cuts at: 2126 2126
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5191
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2029 2683 2920 3101 4646 1132
Size: 97 800 654 237 181 1545 2229
Fragments arranged by size:
2229 1545 800 654 237 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 4363
PfoI T'CCnGG_A
Cuts at: 2450 2450
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2485 2785 2485
Size: 300 5443
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2196 2350 2872 2196
Size: 154 522 5067
Fragments arranged by size:
5067 522 154
PstI C_TGCA'G
Cuts at: 830 2517 3095 830
Size: 1687 578 3478
Fragments arranged by size:
3478 1687 578
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2906 2974 2906
Size: 68 5675
SacI G_AGCT'C
Cuts at: 2370 2466 2370
Size: 96 5647
SacII CC_GC'GG
Cuts at: 2195 2228 2195
Size: 33 5710
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SapI GCTCTTCn'nnn_
Cuts at: 2588 2588
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2177 3991 5703 2177
Size: 1814 1712 2217
Fragments arranged by size:
2217 1814 1712
SexAI A'CCwGG_T
Cuts at: 2481 2481
Size: 5743
SfcI C'TryA_G
Cuts at: 826 1045 2513 3036 3091 4226 4907 5098 826
Size: 219 1468 523 55 1135 681 191 1471
Fragments arranged by size:
1471 1468 1135 681 523 219 191 55
SfoI GGC'GCC
Cuts at: 2127 2127
Size: 5743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2078 2096 2150 2201 2271 3070 1872
Size: 206 18 54 51 70 799 4545
Fragments arranged by size:
4545 799 206 70 54 51 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2009 2371 3847 4748 5010 820
Size: 197 926 66 362 1476 901 262 1553
Fragments arranged by size:
1553 1476 926 901 362 262 197 66
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 2920 3101 1132
Size: 1788 181 3774
Fragments arranged by size:
3774 1788 181
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2712 1517
Size: 1195 4548
StyI C'CwwG_G
Cuts at: 513 1065 2805 2965 3182 513
Size: 552 1740 160 217 3074
Fragments arranged by size:
3074 1740 552 217 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3989 5701
Size: 274 80 33 51 1443 198 1814 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1814 1712 1443 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 2076 2094 2148 2199 2269 3068 1870
Size: 206 18 54 51 70 799 4545
Fragments arranged by size:
4545 799 206 70 54 51 18
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SfiI SrfI SwaI XcmI