Restriction map for
pFN21AE4064
(Circular) (Six-base) MAPSORT of: pFN21AE4064.seq Check: 7643 from: 1 to: 5737
pFN21AE4064 5737 bp
With 182 enzymes: SgfI *
March 28, 2012 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2914 2985 2914
Size: 71 5666
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 3800 5184 5452 1632
Size: 983 1185 1384 268 1917
Fragments arranged by size:
1917 1384 1185 983 268
AfeI AGC'GCT
Cuts at: 2021 2192 2021
Size: 171 5566
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 2913 4636 5613 1203
Size: 1710 1723 977 1327
Fragments arranged by size:
1723 1710 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaI G_GGCC'C
Cuts at: 2257 2656 2968 2257
Size: 399 312 5026
Fragments arranged by size:
5026 399 312
ApaLI G'TGCA_C
Cuts at: 3012 3794 4950 3012
Size: 782 1156 3799
Fragments arranged by size:
3799 1156 782
ApoI r'AATT_y
Cuts at: 1493 2476 3046 3272 3424 5533 1493
Size: 983 570 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 570 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 2615 2984 3062 1870
Size: 73 672 369 78 4545
Fragments arranged by size:
4545 672 369 78 73
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2439 3024 3058 4513 618
Size: 325 217 627 652 585 34 1455 1842
Fragments arranged by size:
1842 1455 652 627 585 325 217 34
BanII G_rGCy'C
Cuts at: 1391 2257 2656 2968 3056 1391
Size: 866 399 312 88 4072
Fragments arranged by size:
4072 866 399 312 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 2603 928
Size: 971 491 213 4062
Fragments arranged by size:
4062 971 491 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3928 3962 1903
Size: 34 50 34 1907 34 3678
Fragments arranged by size:
3678 1907 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 3154 3154
Size: 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2230 2257 2442 2656 2968 3016 3027 3798
Size: 440 27 185 214 312 48 11 771
Cuts at: 3798 4954 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 771 440 312 214 185 48
27 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5737
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2086 2237 2285 2345 4397 1239
Size: 847 151 48 60 2052 2579
Fragments arranged by size:
2579 2052 847 151 60 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4727 5025 3862
Size: 865 298 4574
Fragments arranged by size:
4574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4400 882
Size: 617 2901 2219
Fragments arranged by size:
2901 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2942 3528 493
Size: 711 1738 586 2702
Fragments arranged by size:
2702 1738 711 586
BsaBI GATnn'nnATC
Cuts at: 5731 5731
Size: 5737
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2166 2331 3544
Size: 53 83 186 936 546 87 165 1213
Cuts at: 3544 3926 4561 275
Size: 382 635 1451
Fragments arranged by size:
1451 1213 936 635 546 382 186 165
87 83 53
BsaWI w'CCGG_w
Cuts at: 2199 2352 2379 4170 4568 4842 4989 5400 2199
Size: 153 27 1791 398 274 147 411 2536
Fragments arranged by size:
2536 1791 411 398 274 153 147 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5512
Fragments arranged by size:
5512 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5737
BseYI C'CCAG_C
Cuts at: 2587 4940 5436 2587
Size: 2353 496 2888
Fragments arranged by size:
2888 2353 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2922 1163
Size: 573 1186 3978
Fragments arranged by size:
3978 1186 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 3016 3798 3883 4954 2223
Size: 793 782 85 1071 3006
Fragments arranged by size:
3006 1071 793 782 85
BsiWI C'GTAC_G
Cuts at: 2046 2046
Size: 5737
BsmI GAATG_Cn'
Cuts at: 3248 3341 3248
Size: 93 5644
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 2672
Size: 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2230 2257 2442 2656 2968 3016
Size: 399 433 7 27 185 214 312 48
Cuts at: 3016 3027 3056 3798 3883 4954 1391
Size: 11 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 312 214 185
85 48 29 27 11 7
BspEI T'CCGG_A
Cuts at: 2199 2379 5400 2199
Size: 180 3021 2536
Fragments arranged by size:
3021 2536 180
BspHI T'CATG_A
Cuts at: 3626 5356 3626
Size: 1730 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2914 2985 3092 844
Size: 2070 71 107 3489
Fragments arranged by size:
3489 2070 107 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 3624 1394
Size: 429 236 1565 3507
Fragments arranged by size:
3507 1565 429 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 4232 4406 65
Size: 1183 2984 174 1396
Fragments arranged by size:
2984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2641 4381 4568 1700
Size: 110 831 1740 187 2869
Fragments arranged by size:
2869 1740 831 187 110
BsrGI T'GTAC_A
Cuts at: 96 2415 96
Size: 2319 3418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5737
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2110
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 2751 3067 3434 3819 3836 5288 5374 5386
Size: 779 316 367 385 17 1452 86 12
Cuts at: 5386 5732 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 779 385 367 346 316 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2064 2064
Size: 5737
BtgI C'CryG_G
Cuts at: 513 1065 2388 2733 513
Size: 552 1323 345 3517
Fragments arranged by size:
3517 1323 552 345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 4047 4067 3253
Size: 794 20 4923
Fragments arranged by size:
4923 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2609 4744 809
Size: 507 1293 2135 1802
Fragments arranged by size:
2135 1802 1293 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 3184 4073
Size: 54 1425 50 275 18 750 604 889
Cuts at: 4073 5702 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 750 604 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3667 1353
Size: 2314 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2237 2285 2345 2615 3800 4397
Size: 393 454 151 48 60 270 1185 597
Cuts at: 4397 5184 5452 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1185 787 597 454 393 270 268
151 60 48
EcoO109I rG'GnC_Cy
Cuts at: 2029 2209 2253 2409 2611 2652 2653 2964 2965
Size: 180 44 156 202 41 1 311 1
Cuts at: 2965 2029
Size: 4801
Fragments arranged by size:
4801 311 202 180 156 44 41 1
1
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
EcoRV GAT'ATC
Cuts at: 2492 2492
Size: 5737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 2023 2194 4884 1678
Size: 345 171 2690 2531
Fragments arranged by size:
2690 2531 345 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2650 2682 2893 2925 4378
Size: 32 135 32 1078 32 211 32 1453
Cuts at: 4378 4410 4452 4484 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1453 1078 211 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2530 4851 5035 1305
Size: 1225 2321 184 2007
Fragments arranged by size:
2321 2007 1225 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2918 3695 4054 4213 518
Size: 648 1752 777 359 159 2042
Fragments arranged by size:
2042 1752 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2215 2700 3153 3830 4978 5223 1606
Size: 609 485 453 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 609 485 453 245
NaeI GCC'GGC
Cuts at: 2643 2643
Size: 5737
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5185
NdeI CA'TA_TG
Cuts at: 387 2434 387
Size: 2047 3690
NgoMIV G'CCGG_C
Cuts at: 2641 2641
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 3095 4640 1132
Size: 97 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 97
PacI TTA_AT'TAA
Cuts at: 2814 2814
Size: 5737
PasI CC'CwG_GG
Cuts at: 1348 2959 1348
Size: 1611 4126
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2455 1108
Size: 1347 4390
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PmlI CAC'GTG
Cuts at: 2942 2942
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2029 2209 2409 2611 2029
Size: 180 200 202 5155
Fragments arranged by size:
5155 202 200 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 2504 3307 2504
Size: 803 4934
PspOMI G'GGCC_C
Cuts at: 2253 2652 2964 2253
Size: 399 312 5026
Fragments arranged by size:
5026 399 312
PstI C_TGCA'G
Cuts at: 830 2157 2982 3089 830
Size: 1327 825 107 3478
Fragments arranged by size:
3478 1327 825 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SanDI GG'GwC_CC
Cuts at: 2029 2209 2029
Size: 180 5557
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5737
SfcI C'TryA_G
Cuts at: 826 1045 2153 2572 2978 3085 4220 4901 5092
Size: 219 1108 419 406 107 1135 681 191
Cuts at: 5092 826
Size: 1471
Fragments arranged by size:
1471 1135 1108 681 419 406 219 191
107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 2986 3064 1872
Size: 1114 78 4545
Fragments arranged by size:
4545 1114 78
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3841 4742 5004 820
Size: 197 926 1898 901 262 1553
Fragments arranged by size:
1898 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 3176 513
Size: 552 2111 3074
Fragments arranged by size:
3074 2111 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 3764 3781 3934 4119 4564 325
Size: 1255 1035 1149 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1149 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 3983 5695
Size: 274 80 33 51 1443 438 1568 1712
Cuts at: 5695 96
Size: 138
Fragments arranged by size:
1712 1568 1443 438 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2836 2868 5697 5729 2836
Size: 32 2829 32 2844
Fragments arranged by size:
2844 2829 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 3073 3073
Size: 5737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 2984 3062 1870
Size: 1114 78 4545
Fragments arranged by size:
4545 1114 78
XmnI GAAnn'nnTTC
Cuts at: 2096 3866 2096
Size: 1770 3967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI
NarI NruI NsiI PfoI PspXI PsrI PvuII RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI XcmI