Restriction map for
pFN21AE4062
(Circular) (Six-base) MAPSORT of: pFN21AE4062.seq Check: 6912 from: 1 to: 5605
pFN21AE4062 5605 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2740 2740
Size: 5605
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 3668 5052 5320 1632
Size: 477 1559 1384 268 1917
Fragments arranged by size:
1917 1559 1384 477 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 2484 1166
Size: 1318 4287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 2591 2713 2755 3348 4920 2591
Size: 122 42 593 1572 3276
Fragments arranged by size:
3276 1572 593 122 42
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2099 2914 3140 3292 5401 1493
Size: 606 815 226 152 2109 1697
Fragments arranged by size:
2109 1697 815 606 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 1870
Size: 73 987 4545
Fragments arranged by size:
4545 987 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2862 2924 1391
Size: 1471 62 4072
Fragments arranged by size:
4072 1471 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2798 2832 3796 3830 1903
Size: 34 861 34 964 34 3678
Fragments arranged by size:
3678 964 861 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 2535 1548
Size: 987 4618
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 2525 2562 2856 3022 2525
Size: 37 294 166 5108
Fragments arranged by size:
5108 294 166 37
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4822 1790
Size: 1876 1156 2573
Fragments arranged by size:
2573 1876 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2680 4265 1239
Size: 1441 1585 2579
Fragments arranged by size:
2579 1585 1441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2432 4268 882
Size: 617 933 1836 2219
Fragments arranged by size:
2219 1836 933 617
BsaAI yAC'GTr
Cuts at: 493 1204 2267 3396 493
Size: 711 1063 1129 2702
Fragments arranged by size:
2702 1129 1063 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2232 4038 4436 4710 4857 5268 2232
Size: 1806 398 274 147 411 2569
Fragments arranged by size:
2569 1806 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2002 2032 1178
Size: 30 165 30 599 30 4751
Fragments arranged by size:
4751 599 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2179 1186
Size: 993 4612
BseYI C'CCAG_C
Cuts at: 4808 5304 4808
Size: 496 5109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2213 2604 2615 1163
Size: 573 318 159 391 11 4153
Fragments arranged by size:
4153 573 391 318 159 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2430 2568 2862 3666 3751 4822 2430
Size: 138 294 804 85 1071 3213
Fragments arranged by size:
3213 1071 804 294 138 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 2568 2862 2924 3666 3751 4822
Size: 399 640 138 294 62 742 85 1071
Cuts at: 4822 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 640 399 294 138 85
62
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2235 2740 2960 844
Size: 1391 505 220 3489
Fragments arranged by size:
3489 1391 505 220
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 3492 1394
Size: 429 320 1349 3507
Fragments arranged by size:
3507 1349 429 320
BsrDI GCAATG_nn'
Cuts at: 65 1248 2259 4100 4274 65
Size: 1183 1011 1841 174 1396
Fragments arranged by size:
1841 1396 1183 1011 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 2435 3665 4677 2435
Size: 1230 1012 3363
Fragments arranged by size:
3363 1230 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2740 2755 794
Size: 1946 15 3644
Fragments arranged by size:
3644 1946 15
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2293 2337 2935 3302 3687 3704 5156 5242
Size: 321 44 598 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 598 385 367 346 321 86
44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5053
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 3121 3915 3935 3121
Size: 794 20 4791
Fragments arranged by size:
4791 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3052 3941
Size: 54 1425 50 275 18 675 547 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 675 547 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 2414 2609 3535 1353
Size: 965 96 195 926 3423
Fragments arranged by size:
3423 965 926 195 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2860 2860
Size: 5605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2680 3668 4265 5052 5320 1239
Size: 393 477 571 988 597 787 268 1524
Fragments arranged by size:
1524 988 787 597 571 477 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2080 2080
Size: 5605
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 5605
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373
Size: 32 135 32 2674 32 42 32 2626
Fragments arranged by size:
2674 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2507 10
Size: 54 1425 343 675 3108
Fragments arranged by size:
3108 1425 675 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2484 2501 3563 3922 4081 518
Size: 648 1318 17 1062 359 159 2042
Fragments arranged by size:
2042 1318 1062 648 359 159 17
MspA1I CmG'CkG
Cuts at: 1606 2416 2484 3021 3698 4846 5091 1606
Size: 810 68 537 677 1148 245 2120
Fragments arranged by size:
2120 1148 810 677 537 245 68
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5053
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2524 2731 2963 4508 1132
Size: 97 1295 207 232 1545 2229
Fragments arranged by size:
2229 1545 1295 232 207 97
PasI CC'CwG_GG
Cuts at: 1348 2299 1348
Size: 951 4654
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2419 2713 1108
Size: 1311 294 4000
Fragments arranged by size:
4000 1311 294
PfoI T'CCnGG_A
Cuts at: 2544 2544
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PmlI CAC'GTG
Cuts at: 2267 2267
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2469 2699 2736 2760 2957 830
Size: 1639 230 37 24 197 3478
Fragments arranged by size:
3478 1639 230 197 37 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2416 2484 2416
Size: 68 5537
SacI G_AGCT'C
Cuts at: 2862 2862
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SapI GCTCTTCn'nnn_
Cuts at: 2414 2414
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2699 2957 2699
Size: 258 5347
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2465 2695 2722 2732 2756 2953 4088
Size: 219 1420 230 27 10 24 197 1135
Cuts at: 4088 4769 4960 826
Size: 681 191 1471
Fragments arranged by size:
1471 1420 1135 681 230 219 197 191
27 24 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2731 2963 1132
Size: 1599 232 3774
Fragments arranged by size:
3774 1599 232
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 2183 1517
Size: 666 4939
StyI C'CwwG_G
Cuts at: 513 1065 3044 513
Size: 552 1979 3074
Fragments arranged by size:
3074 1979 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3632 3649 3802 3987 4432 325
Size: 1255 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2002 2034 5565 5597 2002
Size: 32 3531 32 2010
Fragments arranged by size:
3531 2010 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI