Restriction map for
pFN21AE4061
(Circular) (Six-base) MAPSORT of: pFN21AE4061.seq Check: 702 from: 1 to: 5707
pFN21AE4061 5707 bp
With 182 enzymes: SgfI *
March 28, 2012 13:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 3770 5154 5422 1632
Size: 662 1476 1384 268 1917
Fragments arranged by size:
1917 1476 1384 662 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2599 3450 5022 2599
Size: 851 1572 3284
Fragments arranged by size:
3284 1572 851
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2057 2078 2432 2654 2852 2897 2918 3016
Size: 564 21 354 222 198 45 21 98
Cuts at: 3016 3242 3394 5503 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 564 354 226 222 198 152
98 45 21 21
AseI AT'TA_AT
Cuts at: 160 1032 2274 4261 160
Size: 872 1242 1987 1606
Fragments arranged by size:
1987 1606 1242 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2770 3026 1391
Size: 1379 256 4072
Fragments arranged by size:
4072 1379 256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4736
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BfrBI ATG'CAT
Cuts at: 2848 2848
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 2589 3124 2589
Size: 535 5172
Bme1580I G_kGCm'C
Cuts at: 1790 2972 3768 4924 1790
Size: 1182 796 1156 2573
Fragments arranged by size:
2573 1182 1156 796
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4367 1239
Size: 3128 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 2296 4910 5406 2296
Size: 2614 496 2597
Fragments arranged by size:
2614 2597 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2770 3768 3853 4924 2770
Size: 998 85 1071 3553
Fragments arranged by size:
3553 1071 998 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2770 2972 3026 3768 3853 4924 1391
Size: 399 980 202 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 980 742 399 202 85 54
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2584 3062 844
Size: 1740 478 3489
Fragments arranged by size:
3489 1740 478
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 2547 96
Size: 2451 3256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2121 3037 3404 3789 3806 5258 5344 5356
Size: 149 916 367 385 17 1452 86 12
Cuts at: 5356 5702 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 916 385 367 346 149 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2883 513
Size: 552 1818 3337
Fragments arranged by size:
3337 1818 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2483 3223 4017 4037 2483
Size: 740 794 20 4153
Fragments arranged by size:
4153 794 740 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672
Size: 54 1425 50 275 18 1324 889 1629
Cuts at: 5672 8
Size: 43
Fragments arranged by size:
1629 1425 1324 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 2616 2868 3637 1353
Size: 960 303 252 769 3423
Fragments arranged by size:
3423 960 769 303 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2768 2768
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 3770 4367 5154 5422 1239
Size: 393 662 1476 597 787 268 1524
Fragments arranged by size:
1524 1476 787 662 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2596 2878 2596
Size: 282 5425
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
EcoRV GAT'ATC
Cuts at: 2254 2632 2254
Size: 378 5329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2432 2432
Size: 5707
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 2071 2966 4854 1678
Size: 393 895 1888 2531
Fragments arranged by size:
2531 1888 895 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 2697 2715 3306 2697
Size: 18 591 5098
Fragments arranged by size:
5098 591 18
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2831 4821 5005 1305
Size: 1526 1990 184 2007
Fragments arranged by size:
2007 1990 1526 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4024 4183 518
Size: 648 2499 359 159 2042
Fragments arranged by size:
2499 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2296 2763 2944 3123 3800 4948 5193 1606
Size: 690 467 181 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 690 677 467 245 181 179
NcoI C'CATG_G
Cuts at: 513 1065 2883 513
Size: 552 1818 3337
Fragments arranged by size:
3337 1818 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NsiI A_TGCA'T
Cuts at: 2850 2850
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2596 2878 2596
Size: 282 5425
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 2130 3277 2130
Size: 1147 4560
PstI C_TGCA'G
Cuts at: 830 2949 3059 830
Size: 2119 110 3478
Fragments arranged by size:
3478 2119 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2296 2944 2296
Size: 648 5059
SacI G_AGCT'C
Cuts at: 2770 2770
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 2244 2945 3055 4190 4871 5062 826
Size: 219 1199 701 110 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 701 681 219 191 110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 2381 1517
Size: 864 4843
StyI C'CwwG_G
Cuts at: 513 1065 2497 2883 3146 513
Size: 552 1432 386 263 3074
Fragments arranged by size:
3074 1432 552 386 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 3751 3904 4089 4534 325
Size: 1255 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 3953 5665
Size: 274 80 33 51 1443 570 1406 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1443 1406 570 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 2150 3043 2150
Size: 893 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2452 2890 2452
Size: 438 5269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 3836 2012
Size: 1824 3883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI