Restriction map for
pFN21AE4057
(Circular) (Six-base) MAPSORT of: pFN21AE4057.seq Check: 7389 from: 1 to: 5596
pFN21AE4057 5596 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2701 2701
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3659 5043 5311 1632
Size: 2027 1384 268 1917
Fragments arranged by size:
2027 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2117 2312 1184
Size: 933 195 4468
Fragments arranged by size:
4468 933 195
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2327 2359 2510 2542 2871 2903 2327
Size: 32 151 32 329 32 5020
Fragments arranged by size:
5020 329 151 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2149 3339 4911 2149
Size: 1190 1572 2834
Fragments arranged by size:
2834 1572 1190
ApaI G_GGCC'C
Cuts at: 2265 2265
Size: 5596
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2921 1870
Size: 73 978 4545
Fragments arranged by size:
4545 978 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 2917 4372 618
Size: 325 217 627 1058 72 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 217 72
BanII G_rGCy'C
Cuts at: 1391 2265 2466 2589 2615 2915 1391
Size: 874 201 123 26 300 4072
Fragments arranged by size:
4072 874 300 201 123 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BbvCI CC'TCA_GC
Cuts at: 2174 2316 2174
Size: 142 5454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3787 3821 1903
Size: 34 50 34 1766 34 3678
Fragments arranged by size:
3678 1766 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 4207 136
Size: 107 122 71 631 605 373 2162 1525
Fragments arranged by size:
2162 1525 631 605 373 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 2609 2769 3013 2609
Size: 160 244 5192
Fragments arranged by size:
5192 244 160
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2265 3657 4813 1790
Size: 333 142 1392 1156 2573
Fragments arranged by size:
2573 1392 1156 333 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2805 2805
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2675 2858 4256 1239
Size: 1112 324 183 1398 2579
Fragments arranged by size:
2579 1398 1112 324 183
Bpu10I CC'TnA_GC
Cuts at: 2174 2316 2174
Size: 142 5454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 2482 4259 882
Size: 617 714 269 1777 2219
Fragments arranged by size:
2219 1777 714 617 269
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 2369 5590 2369
Size: 3221 2375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3403 3785 4420
Size: 53 83 186 936 479 1391 382 635
Cuts at: 4420 275
Size: 1451
Fragments arranged by size:
1451 1391 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2382 4029 4427 4701 4848 5259 2382
Size: 1647 398 274 147 411 2719
Fragments arranged by size:
2719 1647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2510 2540 1178
Size: 30 165 30 1107 30 4234
Fragments arranged by size:
4234 1107 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2471 2474 2518 2660 2672 2690 2693
Size: 1147 138 3 44 142 12 18 3
Cuts at: 2693 2702 2781 1186
Size: 9 79 4001
Fragments arranged by size:
4001 1147 142 138 79 44 18 12
9 3 3
BseYI C'CCAG_C
Cuts at: 2088 4799 5295 2088
Size: 2711 496 2389
Fragments arranged by size:
2711 2389 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2729 1163
Size: 573 993 4030
Fragments arranged by size:
4030 993 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2021 2074 3046 3807 4835 1540
Size: 275 186 20 53 972 761 1028 2301
Fragments arranged by size:
2301 1028 972 761 275 186 53 20
BsiHKAI G_wGCw'C
Cuts at: 2466 2589 2615 2642 3657 3742 4813 2466
Size: 123 26 27 1015 85 1071 3249
Fragments arranged by size:
3249 1071 1015 123 85 27 26
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2265 2466 2589 2615 2642 2915
Size: 399 333 142 201 123 26 27 273
Cuts at: 2915 3657 3742 4813 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 333 273 201 142
123 85 27 26
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2701 2951 844
Size: 1857 250 3489
Fragments arranged by size:
3489 1857 250
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2056 4240 4427 1700
Size: 110 210 36 2184 187 2869
Fragments arranged by size:
2869 2184 210 187 110 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 2407 1184
Size: 1223 4373
BstXI CCAn_nnnn'nTGG
Cuts at: 2882 2882
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591
Size: 954 367 385 17 1452 86 12 346
Cuts at: 5591 1972
Size: 1977
Fragments arranged by size:
1977 1452 954 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2763 2763
Size: 5596
BtgI C'CryG_G
Cuts at: 513 1065 2015 513
Size: 552 950 4094
Fragments arranged by size:
4094 950 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2039 487
Size: 683 56 813 4044
Fragments arranged by size:
4044 813 683 56
BtsI GCAGTG_nn'
Cuts at: 2453 2784 3112 3906 3926 2453
Size: 331 328 794 20 4123
Fragments arranged by size:
4123 794 331 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 4603 809
Size: 507 1089 2198 1802
Fragments arranged by size:
2198 1802 1089 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 2071 3043
Size: 54 1425 50 275 18 188 53 972
Cuts at: 3043 3932 5561 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 972 889 275 188 54 53
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2018 2071 3043 1537
Size: 275 206 53 972 4090
Fragments arranged by size:
4090 972 275 206 53
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2385 3526 1353
Size: 837 195 1141 3423
Fragments arranged by size:
3423 1141 837 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2464 2587 2613 2464
Size: 123 26 5447
Fragments arranged by size:
5447 123 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2675 2858 3659 4256 5043 5311
Size: 393 719 324 183 801 597 787 268
Cuts at: 5311 1239
Size: 1524
Fragments arranged by size:
1524 801 787 719 597 393 324 268
183
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 2186
Size: 5596
EcoO109I rG'GnC_Cy
Cuts at: 2226 2326 2416 2860 2226
Size: 100 90 444 4962
Fragments arranged by size:
4962 444 100 90
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2054 2503 4743 1678
Size: 376 449 2240 2531
Fragments arranged by size:
2531 2240 449 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2189 2221 2806
Size: 32 135 32 578 32 7 32 585
Cuts at: 2806 2838 4237 4269 4311 4343 1373
Size: 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1399 585 578 135 42 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 4710 4894 1305
Size: 882 2523 184 2007
Fragments arranged by size:
2523 2007 882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3774
Fragments arranged by size:
3774 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2471 2851 3554 3913 4072 518
Size: 648 1305 380 703 359 159 2042
Fragments arranged by size:
2042 1305 703 648 380 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2362 2512 2653 3012 3689 4837 5082
Size: 411 345 150 141 359 677 1148 245
Cuts at: 5082 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 411 359 345 245 150
141
NaeI GCC'GGC
Cuts at: 2022 2058 2022
Size: 36 5560
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NgoMIV G'CCGG_C
Cuts at: 2020 2056 2020
Size: 36 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 2071 3043 2071
Size: 972 4624
NspI r_CATG'y
Cuts at: 1132 1229 2432 2954 4499 1132
Size: 97 1203 522 1545 2229
Fragments arranged by size:
2229 1545 1203 522 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PfoI T'CCnGG_A
Cuts at: 2328 2328
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2542 3665 3697 5178 5210 2510
Size: 32 1123 32 1481 32 2896
Fragments arranged by size:
2896 1481 1123 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2326 2416 2326
Size: 90 5506
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PspOMI G'GGCC_C
Cuts at: 2261 2261
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2205 2448 2948 830
Size: 1375 243 500 3478
Fragments arranged by size:
3478 1375 500 243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2512 2653 2512
Size: 141 5455
RsrII CG'GwC_CG
Cuts at: 2209 2209
Size: 5596
SacI G_AGCT'C
Cuts at: 2466 2589 2615 2466
Size: 123 26 5447
Fragments arranged by size:
5447 123 26
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2190 2190
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2201 2444 2944 4079 4760 4951 826
Size: 219 1156 243 500 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 681 500 243 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 2056 1700
Size: 356 5240
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2580 3035 513
Size: 552 1515 455 3074
Fragments arranged by size:
3074 1515 552 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 2426 2437 3623 3640 3793 3978
Size: 1255 509 337 11 1186 17 153 185
Cuts at: 3978 4423 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1186 509 445 337 185 153
17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 2168 3725 2168
Size: 1557 4039
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI SanDI SexAI
SfiI SfoI SrfI SwaI XcmI