Restriction map for
pFN21AE4056
(Circular) (Six-base) MAPSORT of: pFN21AE4056.seq Check: 5761 from: 1 to: 5635
pFN21AE4056 5635 bp
With 182 enzymes: SgfI *
March 28, 2012 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2716 3445 278
Size: 53 83 186 936 1180 729 2468
Fragments arranged by size:
2468 1180 936 729 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2514 2978 1937
Size: 577 464 4594
Fragments arranged by size:
4594 577 464
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3698 5082 5350 1632
Size: 2066 1384 268 1917
Fragments arranged by size:
2066 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 2451 4534 5511 1203
Size: 1248 2083 977 1327
Fragments arranged by size:
2083 1327 1248 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaI G_GGCC'C
Cuts at: 2389 2930 2389
Size: 541 5094
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2944 3170 3322 5431 1493
Size: 1451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 2438 2582 2960 1870
Size: 73 445 50 144 378 4545
Fragments arranged by size:
4545 445 378 144 73 50
AvrII C'CTAG_G
Cuts at: 2636 2860 2636
Size: 224 5411
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2275 2956 4411 618
Size: 325 217 627 488 681 1455 1842
Fragments arranged by size:
1842 1455 681 627 488 325 217
BanII G_rGCy'C
Cuts at: 1391 2389 2432 2439 2445 2622 2930 2954 1391
Size: 998 43 7 6 177 308 24 4072
Fragments arranged by size:
4072 998 308 177 43 24 7 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2297 2389 2930 3696 4852 1790
Size: 234 273 92 541 766 1156 2573
Fragments arranged by size:
2573 1156 766 541 273 234 92
BmgBI CAC'GTC
Cuts at: 1318 2454 1318
Size: 1136 4499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2239 2392 2408
Size: 416 53 83 399 24 822 153 16
Cuts at: 2408 3366 4324 442
Size: 958 958 1753
Fragments arranged by size:
1753 958 958 822 416 399 153 83
53 24 16
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2774 4295 1239
Size: 1535 1521 2579
Fragments arranged by size:
2579 1535 1521
Bpu10I CC'TnA_GC
Cuts at: 2616 2747 2616
Size: 131 5504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2769 4298 882
Size: 617 1270 1529 2219
Fragments arranged by size:
2219 1529 1270 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2713 3442 3824 4459
Size: 53 83 186 936 1180 729 382 635
Cuts at: 4459 275
Size: 1451
Fragments arranged by size:
1451 1180 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2667 4068 4466 4740 4887 5298 2667
Size: 1401 398 274 147 411 3004
Fragments arranged by size:
3004 1401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2388 2418 2664 2694 1178
Size: 30 165 30 985 30 246 30 4119
Fragments arranged by size:
4119 985 246 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 1186
Size: 1336 4299
BseYI C'CCAG_C
Cuts at: 2256 2297 2533 2563 2759 2824 4838 5334 2256
Size: 41 236 30 196 65 2014 496 2557
Fragments arranged by size:
2557 2014 496 236 196 65 41 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5062
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2720 2777 3085 3846 4874 1540
Size: 275 186 719 57 308 761 1028 2301
Fragments arranged by size:
2301 1028 761 719 308 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2082 3696 3781 4852 2082
Size: 1614 85 1071 2865
Fragments arranged by size:
2865 1614 1071 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
BsmBI CGTCTCn'nnnn_
Cuts at: 2189 2189
Size: 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2082 2297 2389 2432 2439 2445
Size: 399 234 58 215 92 43 7 6
Cuts at: 2445 2622 2930 2954 3696 3781 4852 1391
Size: 177 308 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 234 215 177
92 85 58 43 24 7 6
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 2904 3524 5254 2904
Size: 620 1730 3285
Fragments arranged by size:
3285 1730 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2287 2990 844
Size: 1289 154 703 3489
Fragments arranged by size:
3489 1289 703 154
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2135 2311 4130 4304 65
Size: 1183 887 176 1819 174 1396
Fragments arranged by size:
1819 1396 1183 887 176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2282 2577 2719 4279 4466 1700
Size: 110 472 295 142 1560 187 2869
Fragments arranged by size:
2869 1560 472 295 187 142 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 2055 4439 2055
Size: 2384 3251
BstEII G'GTnAC_C
Cuts at: 1184 2035 1184
Size: 851 4784
BstYI r'GATC_y
Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630
Size: 993 367 385 17 1452 86 12 346
Cuts at: 5630 1972
Size: 1977
Fragments arranged by size:
1977 1452 993 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2343 2683 513
Size: 552 939 339 340 3465
Fragments arranged by size:
3465 939 552 340 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 2080 3151 3945 3965 2080
Size: 1071 794 20 3750
Fragments arranged by size:
3750 1071 794 20
ClaI AT'CG_AT
Cuts at: 1554 2415 1554
Size: 861 4774
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DraIII CAC_nnn'GTG
Cuts at: 2596 2596
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2370 2717
Size: 54 1425 50 275 18 354 186 347
Cuts at: 2717 2774 3082 3971 5600 8
Size: 57 308 889 1629 43
Fragments arranged by size:
1629 1425 889 354 347 308 275 186
57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2717 2774 3082 1537
Size: 275 905 57 308 4090
Fragments arranged by size:
4090 905 308 275 57
EarI CTCTTCn'nnn_
Cuts at: 1353 3565 1353
Size: 2212 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2774 3698 4295 5082 5350 1239
Size: 393 1142 924 597 787 268 1524
Fragments arranged by size:
1524 1142 924 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2551 2927 2551
Size: 376 5259
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FseI GG_CCGG'CC
Cuts at: 2581 2723 2581
Size: 142 5493
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2523 4782 1678
Size: 845 2259 2531
Fragments arranged by size:
2531 2259 845
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 2507 2539 4276
Size: 32 135 32 816 32 87 32 1737
Cuts at: 4276 4308 4350 4382 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1737 816 135 87 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 2380 2979 3225 669
Size: 988 281 130 312 599 246 3079
Fragments arranged by size:
3079 988 599 312 281 246 130
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 2158 3234 2158
Size: 1076 4559
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 2372 10
Size: 54 1425 343 354 186 3273
Fragments arranged by size:
3273 1425 354 343 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3593 3952 4111 518
Size: 648 1755 672 359 159 2042
Fragments arranged by size:
2042 1755 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2301 2779 2794 2876 3051 3728 4876 5121
Size: 695 478 15 82 175 677 1148 245
Cuts at: 5121 1606
Size: 2120
Fragments arranged by size:
2120 1148 695 677 478 245 175 82
15
NaeI GCC'GGC
Cuts at: 2284 2579 2721 2284
Size: 295 142 5198
Fragments arranged by size:
5198 295 142
NcoI C'CATG_G
Cuts at: 513 1065 2004 2683 513
Size: 552 939 679 3465
Fragments arranged by size:
3465 939 679 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 2282 2577 2719 2282
Size: 295 142 5198
Fragments arranged by size:
5198 295 142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 2774 3082 2774
Size: 308 5327
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PspOMI G'GGCC_C
Cuts at: 2385 2926 2385
Size: 541 5094
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2301 2876 2301
Size: 575 5060
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2542 2983 4118 4799 4990 826
Size: 219 1497 441 1135 681 191 1471
Fragments arranged by size:
1497 1471 1135 681 441 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2390 2962 1872
Size: 518 572 4545
Fragments arranged by size:
4545 572 518
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SrfI GCCC'GGGC
Cuts at: 2390 2390
Size: 5635
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 2679 1517
Size: 1162 4473
StyI C'CwwG_G
Cuts at: 513 1065 2004 2636 2683 2724 2860 3074 513
Size: 552 939 632 47 41 136 214 3074
Fragments arranged by size:
3074 939 632 552 214 136 47 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2664 2696 5595 5627 2664
Size: 32 2899 32 2672
Fragments arranged by size:
2899 2672 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2388 2960 1870
Size: 518 572 4545
Fragments arranged by size:
4545 572 518
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2714 3443 276
Size: 53 83 186 936 1180 729 2468
Fragments arranged by size:
2468 1180 936 729 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SwaI XcmI