Restriction map for
pFN21AE4053
(Circular) (Six-base) MAPSORT of: pFN21AE4053.seq Check: 2980 from: 1 to: 5605
pFN21AE4053 5605 bp
With 182 enzymes: SgfI *
March 28, 2012 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2200 2200
Size: 5605
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3668 5052 5320 1632
Size: 2036 1384 268 1917
Fragments arranged by size:
2036 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 2095 4504 5481 1203
Size: 892 2409 977 1327
Fragments arranged by size:
2409 1327 977 892
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5605
AlwNI CAG_nnn'CTG
Cuts at: 2278 2437 2851 3348 4920 2278
Size: 159 414 497 1572 2963
Fragments arranged by size:
2963 1572 497 414 159
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 1870
Size: 73 987 4545
Fragments arranged by size:
4545 987 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 2323 2926 4381 618
Size: 325 217 627 500 36 603 1455 1842
Fragments arranged by size:
1842 1455 627 603 500 325 217 36
BanII G_rGCy'C
Cuts at: 1391 2046 2924 1391
Size: 655 878 4072
Fragments arranged by size:
4072 878 655
BbeI G_GCGC'C
Cuts at: 2291 2291
Size: 5605
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 928
Size: 971 186 4448
Fragments arranged by size:
4448 971 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2369 2403 3796 3830 1903
Size: 34 432 34 1393 34 3678
Fragments arranged by size:
3678 1393 432 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 4216 136
Size: 107 122 71 631 605 568 1976 1525
Fragments arranged by size:
1976 1525 631 605 568 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4822 1790
Size: 1876 1156 2573
Fragments arranged by size:
2573 1876 1156
BmgBI CAC'GTC
Cuts at: 1318 2083 2098 2827 1318
Size: 765 15 729 4096
Fragments arranged by size:
4096 765 729 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2467 3336 4294
Size: 416 53 83 399 24 1050 869 958
Cuts at: 4294 442
Size: 1753
Fragments arranged by size:
1753 1050 958 869 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2528 4265 1239
Size: 1289 1737 2579
Fragments arranged by size:
2579 1737 1289
Bpu10I CC'TnA_GC
Cuts at: 2792 2792
Size: 5605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 3730 4595 4893 2525
Size: 1205 865 298 3237
Fragments arranged by size:
3237 1205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 4268 882
Size: 617 1092 1677 2219
Fragments arranged by size:
2219 1677 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2288 2628 3412 3794
Size: 53 83 186 936 755 340 784 382
Cuts at: 3794 4429 275
Size: 635 1451
Fragments arranged by size:
1451 936 784 755 635 382 340 186
83 53
BsaWI w'CCGG_w
Cuts at: 2027 2157 4038 4436 4710 4857 5268 2027
Size: 130 1881 398 274 147 411 2364
Fragments arranged by size:
2364 1881 411 398 274 147 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 1186
Size: 1339 4266
BseYI C'CCAG_C
Cuts at: 4808 5304 4808
Size: 496 5109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 2825 1163
Size: 573 453 636 3943
Fragments arranged by size:
3943 636 573 453
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2162 3055 3816 4844 1540
Size: 275 186 161 893 761 1028 2301
Fragments arranged by size:
2301 1028 893 761 275 186 161
BsiHKAI G_wGCw'C
Cuts at: 3666 3751 4822 3666
Size: 85 1071 4449
Fragments arranged by size:
4449 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2924 3666 3751 4822 1391
Size: 399 256 878 742 85 1071 2174
Fragments arranged by size:
2174 1071 878 742 399 256 85
BspEI T'CCGG_A
Cuts at: 2027 5268 2027
Size: 3241 2364
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 2200 2890 2904 2960 844
Size: 1232 124 690 14 56 3489
Fragments arranged by size:
3489 1232 690 124 56 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2326 2684 4249 4436 1700
Size: 110 516 358 1565 187 2869
Fragments arranged by size:
2869 1565 516 358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2223 2518 2935 3302 3687 3704 5156 5242
Size: 251 295 417 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 417 385 367 346 295 251
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2228 2631 513
Size: 552 939 224 403 3487
Fragments arranged by size:
3487 939 552 403 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2764 3121 3915 3935 2764
Size: 357 794 20 4434
Fragments arranged by size:
4434 794 357 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2776 2776
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2409 2604
Size: 54 1425 50 275 18 351 228 195
Cuts at: 2604 2724 3052 3941 5570 8
Size: 120 328 889 1629 43
Fragments arranged by size:
1629 1425 889 351 328 275 228 195
120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3535 1353
Size: 2182 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2795 4192 4566 4712 387
Size: 1064 138 1206 1397 374 146 1280
Fragments arranged by size:
1397 1280 1206 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 3668 4265 5052 5320 1239
Size: 393 896 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 896 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2110 2317 2848 2110
Size: 207 531 4867
Fragments arranged by size:
4867 531 207
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2291 2399 2437 2477 2583 4752 1678
Size: 613 108 38 40 106 2169 2531
Fragments arranged by size:
2531 2169 613 108 106 40 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373
Size: 32 135 32 2674 32 42 32 2626
Fragments arranged by size:
2674 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2611 2893 2949 3195 669
Size: 988 281 673 282 56 246 3079
Fragments arranged by size:
3079 988 673 282 281 246 56
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KasI G'GCGC_C
Cuts at: 2287 2287
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2680 4719 4903 1305
Size: 1375 2039 184 2007
Fragments arranged by size:
2039 2007 1375 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3783
Fragments arranged by size:
3783 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2825 3563 3922 4081 518
Size: 648 1659 738 359 159 2042
Fragments arranged by size:
2042 1659 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 2125 2230 2350 2633 2839 3021 3698
Size: 510 9 105 120 283 206 182 677
Cuts at: 3698 4846 5091 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 510 283 245 206 182
120 105 9
NarI GG'CG_CC
Cuts at: 2288 2288
Size: 5605
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2073 1348
Size: 725 4880
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 2851 1108
Size: 1668 75 3862
Fragments arranged by size:
3862 1668 75
PfoI T'CCnGG_A
Cuts at: 2061 2061
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2317 2110
Size: 207 5398
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2139 2274 2433 2865 2901 2957 830
Size: 1309 135 159 432 36 56 3478
Fragments arranged by size:
3478 1309 432 159 135 56 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2125 2350 2125
Size: 225 5380
SacII CC_GC'GG
Cuts at: 2231 2634 2231
Size: 403 5202
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SanDI GG'GwC_CC
Cuts at: 2110 2110
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2901 2957 2901
Size: 56 5549
ScaI AGT'ACT
Cuts at: 2732 3853 5565 2732
Size: 1121 1712 2772
Fragments arranged by size:
2772 1712 1121
SexAI A'CCwGG_T
Cuts at: 2319 2319
Size: 5605
SfcI C'TryA_G
Cuts at: 826 1045 2135 2270 2429 2861 2897 2953 4088
Size: 219 1090 135 159 432 36 56 1135
Cuts at: 4088 4769 4960 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1090 681 432 219 191 159
135 56 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 2240 2240
Size: 5605
SfoI GGC'GCC
Cuts at: 2289 2289
Size: 5605
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 3709 4610 4872 820
Size: 197 926 597 1169 901 262 1553
Fragments arranged by size:
1553 1169 926 901 597 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2004 3044 513
Size: 552 939 1040 3074
Fragments arranged by size:
3074 1040 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2449 3632 3649 3802 3987 4432 325
Size: 1255 869 1183 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1183 869 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 3851 5563
Size: 274 80 33 51 1443 753 1121 1712
Cuts at: 5563 96
Size: 138
Fragments arranged by size:
1712 1443 1121 753 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 2711 1316
Size: 1395 4210
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI
SrfI SwaI