Restriction map for
pFN21AE4049
(Circular) (Six-base) MAPSORT of: pFN21AE4049.seq Check: 9272 from: 1 to: 5539
pFN21AE4049 5539 bp
With 182 enzymes: SgfI *
March 28, 2012 13:04 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2421 3349 278
Size: 53 83 186 936 885 928 2468
Fragments arranged by size:
2468 936 928 885 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 2838 3666 4039 1563
Size: 1275 828 373 3063
Fragments arranged by size:
3063 1275 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3602 4986 5254 1632
Size: 1970 1384 268 1917
Fragments arranged by size:
1970 1917 1384 268
AfeI AGC'GCT
Cuts at: 2683 2683
Size: 5539
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 2667 4438 5415 1203
Size: 1464 1771 977 1327
Fragments arranged by size:
1771 1464 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 2215 3282 4854 2215
Size: 1067 1572 2900
Fragments arranged by size:
2900 1572 1067
ApaI G_GGCC'C
Cuts at: 2233 2233
Size: 5539
ApaLI G'TGCA_C
Cuts at: 2072 3596 4752 2072
Size: 1524 1156 2859
Fragments arranged by size:
2859 1524 1156
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2287 2481 2634 2864 1870
Size: 73 70 274 194 153 230 4545
Fragments arranged by size:
4545 274 230 194 153 73 70
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 2860 4315 618
Size: 325 217 627 342 731 1455 1842
Fragments arranged by size:
1842 1455 731 627 342 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2233 2858 1391
Size: 622 220 625 4072
Fragments arranged by size:
4072 625 622 220
BbeI G_GCGC'C
Cuts at: 2133 2133
Size: 5539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2233 2482 3600 4756 1790
Size: 286 157 249 1118 1156 2573
Fragments arranged by size:
2573 1156 1118 286 249 157
BmgBI CAC'GTC
Cuts at: 1318 2044 1318
Size: 726 4813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2801 4199 1239
Size: 1562 1398 2579
Fragments arranged by size:
2579 1562 1398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2773 3664 4529 4827 2773
Size: 891 865 298 3485
Fragments arranged by size:
3485 891 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2670 3330 493
Size: 711 1466 660 2702
Fragments arranged by size:
2702 1466 711 660
BsaBI GATnn'nnATC
Cuts at: 2606 5533 2606
Size: 2927 2612
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 2418 2769 3346
Size: 53 83 186 936 597 288 351 577
Cuts at: 3346 3728 4363 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 597 577 382 351 288
186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5539
BseYI C'CCAG_C
Cuts at: 2085 2225 4742 5238 2085
Size: 140 2517 496 2386
Fragments arranged by size:
2517 2386 496 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2228 2351 2450 1163
Size: 573 420 72 123 99 4252
Fragments arranged by size:
4252 573 420 123 99 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 3600 3685 4756 2076
Size: 1524 85 1071 2859
Fragments arranged by size:
2859 1524 1071 85
BsmI GAATG_Cn'
Cuts at: 2539 3050 3143 2539
Size: 511 93 4935
Fragments arranged by size:
4935 511 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2076 2233 2482 2858 3600 3685
Size: 399 223 63 157 249 376 742 85
Cuts at: 3685 4756 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 249 223 157
85 63
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 2548 3428 5158 2548
Size: 880 1730 2929
Fragments arranged by size:
2929 1730 880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2894 844
Size: 2050 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 2127 2269 2385 3426 1394
Size: 429 239 65 142 116 1041 3507
Fragments arranged by size:
3507 1041 429 239 142 116 65
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4183 4370 1700
Size: 110 2373 187 2869
Fragments arranged by size:
2869 2373 187 110
BsrGI T'GTAC_A
Cuts at: 96 2349 96
Size: 2253 3286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 2075 2369 3599 4611 2075
Size: 294 1230 1012 3003
Fragments arranged by size:
3003 1230 1012 294
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2215 794
Size: 1421 4118
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 2146 1184
Size: 962 4577
BstYI r'GATC_y
Cuts at: 1972 2869 3236 3621 3638 5090 5176 5188 5534
Size: 897 367 385 17 1452 86 12 346
Cuts at: 5534 1972
Size: 1977
Fragments arranged by size:
1977 1452 897 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2644 513
Size: 552 939 147 493 3408
Fragments arranged by size:
3408 939 552 493 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2289 487
Size: 683 56 1063 3737
Fragments arranged by size:
3737 1063 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3849 3869 3055
Size: 794 20 4725
Fragments arranged by size:
4725 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2986 3875
Size: 54 1425 50 275 18 741 415 889
Cuts at: 3875 5504 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 741 415 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 1353
Size: 2116 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 4126 4500 4646 387
Size: 1064 138 447 2090 374 146 1280
Fragments arranged by size:
2090 1280 1064 447 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2801 3602 4199 4986 5254 1239
Size: 393 1169 801 597 787 268 1524
Fragments arranged by size:
1524 1169 801 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2369
Size: 5539
EcoO109I rG'GnC_Cy
Cuts at: 2229 2457 2229
Size: 228 5311
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2133 2685 4686 1678
Size: 455 552 2001 2531
Fragments arranged by size:
2531 2001 552 455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 4180 4212 4254
Size: 32 135 32 1241 32 1335 32 42
Cuts at: 4254 4286 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 1241 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 2590 2883 3129 669
Size: 988 281 400 252 293 246 3079
Fragments arranged by size:
3079 988 400 293 281 252 246
HindIII A'AGCT_T
Cuts at: 748 2543 748
Size: 1795 3744
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2129 2129
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4653 4837 1305
Size: 3348 184 2007
Fragments arranged by size:
3348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 3497 3856 4015 518
Size: 648 1434 897 359 159 2042
Fragments arranged by size:
2042 1434 897 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2107 2116 2212 2646 2955 3632 4780
Size: 483 18 9 96 434 309 677 1148
Cuts at: 4780 5025 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 483 434 309 245 96
18 9
NarI GG'CG_CC
Cuts at: 2130 2130
Size: 5539
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PmlI CAC'GTG
Cuts at: 2670 2670
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2406 3608 3640 5121 5153 2374
Size: 32 1202 32 1481 32 2760
Fragments arranged by size:
2760 1481 1202 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2457 2457
Size: 5539
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PspOMI G'GGCC_C
Cuts at: 2229 2229
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2106 2190 2744 2891 830
Size: 1276 84 554 147 3478
Fragments arranged by size:
3478 1276 554 147 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2107 2116 2212 2107
Size: 9 96 5434
Fragments arranged by size:
5434 96 9
SacII CC_GC'GG
Cuts at: 2647 2647
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SanDI GG'GwC_CC
Cuts at: 2457 2457
Size: 5539
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 2705 3787 5499 2705
Size: 1082 1712 2745
Fragments arranged by size:
2745 1712 1082
SexAI A'CCwGG_T
Cuts at: 2142 2142
Size: 5539
SfcI C'TryA_G
Cuts at: 826 1045 2102 2186 2740 2887 4022 4703 4894
Size: 219 1057 84 554 147 1135 681 191
Cuts at: 4894 826
Size: 1471
Fragments arranged by size:
1471 1135 1057 681 554 219 191 147
84
SfoI GGC'GCC
Cuts at: 2131 2131
Size: 5539
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2015 2483 2636 2866 1872
Size: 143 468 153 230 4545
Fragments arranged by size:
4545 468 230 153 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 3643 4544 4806 820
Size: 197 926 809 891 901 262 1553
Fragments arranged by size:
1553 926 901 891 809 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 1517
Size: 858 4681
StyI C'CwwG_G
Cuts at: 513 1065 2004 2331 2574 2978 513
Size: 552 939 327 243 404 3074
Fragments arranged by size:
3074 939 552 404 327 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 3566 3583 3736 3921 4366 325
Size: 1255 464 1522 17 153 185 445 1498
Fragments arranged by size:
1522 1498 1255 464 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 2703 3785
Size: 274 80 33 51 1443 372 354 1082
Cuts at: 3785 5497 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1082 372 354 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 2498 2728 1316
Size: 1182 230 4127
Fragments arranged by size:
4127 1182 230
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2013 2481 2634 2864 1870
Size: 143 468 153 230 4545
Fragments arranged by size:
4545 468 230 153 143
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2419 3347 276
Size: 53 83 186 936 885 928 2468
Fragments arranged by size:
2468 936 928 885 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI RsrII SacI SapI SfiI
SrfI SwaI XcmI