Restriction map for
pFN21AE4046
(Circular) (Six-base) MAPSORT of: pFN21AE4046.seq Check: 9214 from: 1 to: 5524
pFN21AE4046 5524 bp
With 182 enzymes: SgfI *
March 28, 2012 13:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2455 3334 278
Size: 53 83 186 936 919 879 2468
Fragments arranged by size:
2468 936 919 879 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 3587 4971 5239 1632
Size: 846 1109 1384 268 1917
Fragments arranged by size:
1917 1384 1109 846 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 2179 4423 5400 1203
Size: 976 2244 977 1327
Fragments arranged by size:
2244 1327 977 976
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2637 2833 3059 3211 5320 1493
Size: 1144 196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1144 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 2517 4078 160
Size: 872 1485 1561 1606
Fragments arranged by size:
1606 1561 1485 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2849 1870
Size: 73 906 4545
Fragments arranged by size:
4545 906 73
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 4300 618
Size: 325 217 627 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2843 1391
Size: 1452 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4135 136
Size: 107 122 71 631 605 337 2126 1525
Fragments arranged by size:
2126 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 3585 4741 1790
Size: 1795 1156 2573
Fragments arranged by size:
2573 1795 1156
BmgBI CAC'GTC
Cuts at: 1318 2182 1318
Size: 864 4660
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2526 4184 1239
Size: 1287 1658 2579
Fragments arranged by size:
2579 1658 1287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2674 3649 4514 4812 2674
Size: 975 865 298 3386
Fragments arranged by size:
3386 975 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2049 4187 882
Size: 617 550 2138 2219
Fragments arranged by size:
2219 2138 617 550
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2319 2452 3331 3713
Size: 53 83 186 936 786 133 879 382
Cuts at: 3713 4348 275
Size: 635 1451
Fragments arranged by size:
1451 936 879 786 635 382 186 133
83 53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5524
BseYI C'CCAG_C
Cuts at: 2668 4727 5223 2668
Size: 2059 496 2969
Fragments arranged by size:
2969 2059 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4951
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 3735 4763 1540
Size: 275 186 973 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3585 3670 4741 3585
Size: 85 1071 4368
Fragments arranged by size:
4368 1071 85
BsmI GAATG_Cn'
Cuts at: 2797 3035 3128 2797
Size: 238 93 5193
Fragments arranged by size:
5193 238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2843 3585 3670 4741 1391
Size: 399 1053 742 85 1071 2174
Fragments arranged by size:
2174 1071 1053 742 399 85
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 2879 844
Size: 1187 848 3489
Fragments arranged by size:
3489 1187 848
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4168 4355 1700
Size: 110 2358 187 2869
Fragments arranged by size:
2869 2358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 2248 2641 4328 2248
Size: 393 1687 3444
Fragments arranged by size:
3444 1687 393
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519
Size: 882 367 385 17 1452 86 12 346
Cuts at: 5519 1972
Size: 1977
Fragments arranged by size:
1977 1452 882 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 513
Size: 552 939 63 3970
Fragments arranged by size:
3970 939 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2411 487
Size: 683 56 1185 3600
Fragments arranged by size:
3600 1185 683 56
BtsI GCAGTG_nn'
Cuts at: 2602 2770 3040 3834 3854 2602
Size: 168 270 794 20 4272
Fragments arranged by size:
4272 794 270 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DraIII CAC_nnn'GTG
Cuts at: 2276 2276
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2165 4531 809
Size: 507 849 2366 1802
Fragments arranged by size:
2366 1802 849 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2971 3860 5489
Size: 54 1425 50 275 18 1141 889 1629
Cuts at: 5489 8
Size: 43
Fragments arranged by size:
1629 1425 1141 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3454 1353
Size: 2101 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 2526 3587 4184 4971 5239 1239
Size: 393 846 48 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 846 787 597 393 268 48
EcoO109I rG'GnC_Cy
Cuts at: 2685 2685
Size: 5524
EcoRI G'AATT_C
Cuts at: 2637 2833 3211 2637
Size: 196 378 4950
Fragments arranged by size:
4950 378 196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2471 2471
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 4671 1678
Size: 2993 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373
Size: 32 135 32 2593 32 42 32 2626
Fragments arranged by size:
2626 2593 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 2868 3114 669
Size: 988 281 268 662 246 3079
Fragments arranged by size:
3079 988 662 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 2206 3114 2206
Size: 908 4616
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 2148 3123 2148
Size: 975 4549
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3702
Fragments arranged by size:
3702 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2430 3482 3841 4000 518
Size: 648 900 364 1052 359 159 2042
Fragments arranged by size:
2042 1052 900 648 364 359 159
MspA1I CmG'CkG
Cuts at: 1606 2940 3617 4765 5010 1606
Size: 1334 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2067 513
Size: 552 939 63 3970
Fragments arranged by size:
3970 939 552 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2020 2668 2882 4427 1132
Size: 97 791 648 214 1545 2229
Fragments arranged by size:
2229 1545 791 648 214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 2102 3094 2102
Size: 992 4532
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SfcI C'TryA_G
Cuts at: 826 1045 2872 4007 4688 4879 826
Size: 219 1827 1135 681 191 1471
Fragments arranged by size:
1827 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2689 3628 4529 4791 820
Size: 197 926 746 939 901 262 1553
Fragments arranged by size:
1553 939 926 901 746 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 2383 3448 5
Size: 47 2331 1065 2081
Fragments arranged by size:
2331 2081 1065 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5524
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 2963 513
Size: 552 939 63 896 3074
Fragments arranged by size:
3074 939 896 552 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3551 3568 3721 3906 4351 325
Size: 1255 1971 17 153 185 445 1498
Fragments arranged by size:
1971 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 3770 5482
Size: 274 80 33 51 1443 504 1289 1712
Cuts at: 5482 96
Size: 138
Fragments arranged by size:
1712 1443 1289 504 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 2123 3653 2123
Size: 1530 3994
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2453 3332 276
Size: 53 83 186 936 919 879 2468
Fragments arranged by size:
2468 936 919 879 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI