Restriction map for
pFN21AE4044
(Circular) (Six-base) MAPSORT of: pFN21AE4044.seq Check: 2190 from: 1 to: 5506
pFN21AE4044 5506 bp
With 182 enzymes: SgfI *
March 28, 2012 13:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3316 278
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2606 3569 4953 5221 1632
Size: 974 963 1384 268 1917
Fragments arranged by size:
1917 1384 974 963 268
AfeI AGC'GCT
Cuts at: 2617 2617
Size: 5506
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 4405 5382 1203
Size: 3202 977 1327
Fragments arranged by size:
3202 1327 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 5474
AlwNI CAG_nnn'CTG
Cuts at: 2059 3249 4821 2059
Size: 1190 1572 2744
Fragments arranged by size:
2744 1572 1190
ApaI G_GGCC'C
Cuts at: 2131 2239 2445 2131
Size: 108 206 5192
Fragments arranged by size:
5192 206 108
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2815 3041 3193 5302 1493
Size: 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2238 2831 1870
Size: 73 138 157 593 4545
Fragments arranged by size:
4545 593 157 138 73
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2136 2491 2555 2827 4282
Size: 325 217 627 349 355 64 272 1455
Cuts at: 4282 618
Size: 1842
Fragments arranged by size:
1842 1455 627 355 349 325 272 217
64
BanII G_rGCy'C
Cuts at: 1391 2131 2239 2445 2452 2825 1391
Size: 740 108 206 7 373 4072
Fragments arranged by size:
4072 740 373 206 108 7
BbeI G_GCGC'C
Cuts at: 2140 2140
Size: 5506
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3697 3731 1903
Size: 34 1760 34 3678
Fragments arranged by size:
3678 1760 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2469 3398 4608 2469
Size: 929 1210 3367
Fragments arranged by size:
3367 1210 929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4117 136
Size: 107 122 71 631 605 2445 1525
Fragments arranged by size:
2445 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2923 2923
Size: 5506
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2239 2309 2445 2558 3567 4723 1790
Size: 341 108 70 136 113 1009 1156 2573
Fragments arranged by size:
2573 1156 1009 341 136 113 108 70
BmgBI CAC'GTC
Cuts at: 1318 2687 1318
Size: 1369 4137
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2449 3237 4195
Size: 416 53 83 399 24 1032 788 958
Cuts at: 4195 442
Size: 1753
Fragments arranged by size:
1753 1032 958 788 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 4166 1239
Size: 1268 1659 2579
Fragments arranged by size:
2579 1659 1268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 4496 4794 3631
Size: 865 298 4343
Fragments arranged by size:
4343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4169 882
Size: 617 2670 2219
Fragments arranged by size:
2670 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3297 493
Size: 711 2093 2702
Fragments arranged by size:
2702 2093 711
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2137 3313 3695 4330
Size: 53 83 186 936 604 1176 382 635
Cuts at: 4330 275
Size: 1451
Fragments arranged by size:
1451 1176 936 635 604 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5281
Fragments arranged by size:
5281 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 1186
Size: 1354 4152
BseYI C'CCAG_C
Cuts at: 2130 2374 2400 2795 4709 5205 2130
Size: 244 26 395 1914 496 2431
Fragments arranged by size:
2431 1914 496 395 244 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2091 2252 1163
Size: 573 355 161 4417
Fragments arranged by size:
4417 573 355 161
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2956 3717 4745 1540
Size: 275 186 955 761 1028 2301
Fragments arranged by size:
2301 1028 955 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2228 3567 3652 4723 2228
Size: 1339 85 1071 3011
Fragments arranged by size:
3011 1339 1071 85
BsmI GAATG_Cn'
Cuts at: 2573 3017 3110 2573
Size: 444 93 4969
Fragments arranged by size:
4969 444 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2228 2239 2309 2445 2452 2558
Size: 399 341 97 11 70 136 7 106
Cuts at: 2558 2825 3567 3652 4723 1391
Size: 267 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 267 136 106
97 85 70 11 7
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 3395 5125 3395
Size: 1730 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2861 844
Size: 2017 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 3393 1394
Size: 429 383 1187 3507
Fragments arranged by size:
3507 1187 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 4001 4175 65
Size: 1183 2753 174 1396
Fragments arranged by size:
2753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4150 4337 1700
Size: 110 2340 187 2869
Fragments arranged by size:
2869 2340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 3566 4578 3566
Size: 1012 4494
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5506
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5506
BstYI r'GATC_y
Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501
Size: 864 367 385 17 1452 86 12 346
Cuts at: 5501 1972
Size: 1977
Fragments arranged by size:
1977 1452 864 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2140 2214 2316 2331 2578 513
Size: 552 939 136 74 102 15 247 3441
Fragments arranged by size:
3441 939 552 247 136 102 74 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4767
Fragments arranged by size:
4767 683 56
BtsI GCAGTG_nn'
Cuts at: 2721 3022 3816 3836 2721
Size: 301 794 20 4391
Fragments arranged by size:
4391 794 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2810 2810
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2953 3842
Size: 54 1425 50 275 18 498 625 889
Cuts at: 3842 5471 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 625 498 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2953 1537
Size: 275 1141 4090
Fragments arranged by size:
4090 1141 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2740 3436 1353
Size: 735 652 696 3423
Fragments arranged by size:
3423 735 696 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2507 2606 3569 4166 4953 5221 1239
Size: 393 875 99 963 597 787 268 1524
Fragments arranged by size:
1524 963 875 787 597 393 268 99
EcoO109I rG'GnC_Cy
Cuts at: 2056 2127 2235 2263 2441 2595 2056
Size: 71 108 28 178 154 4967
Fragments arranged by size:
4967 178 154 108 71 28
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 4012 4012
Size: 5506
HaeII r_GCGC'y
Cuts at: 1678 2140 2372 2619 4653 1678
Size: 462 232 247 2034 2531
Fragments arranged by size:
2531 2034 462 247 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2587 2619 2717
Size: 32 135 32 515 32 468 32 98
Cuts at: 2717 2749 4147 4179 4221 4253 1373
Size: 32 1398 32 42 32 2626
Fragments arranged by size:
2626 1398 515 468 135 98 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3096 669
Size: 988 281 912 246 3079
Fragments arranged by size:
3079 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KasI G'GCGC_C
Cuts at: 2136 2136
Size: 5506
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2126 4620 4804 1305
Size: 821 2494 184 2007
Fragments arranged by size:
2494 2007 821 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 10
Size: 54 1425 343 498 3186
Fragments arranged by size:
3186 1425 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3464 3823 3982 518
Size: 648 2298 359 159 2042
Fragments arranged by size:
2298 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2286 2347 2465 2485 2580 2922 3599
Size: 528 152 61 118 20 95 342 677
Cuts at: 3599 4747 4992 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 528 342 245 152 118
95 61 20
NarI GG'CG_CC
Cuts at: 2137 2137
Size: 5506
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4015
Fragments arranged by size:
4015 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2315 2864 4409 1132
Size: 97 1086 549 1545 2229
Fragments arranged by size:
2229 1545 1086 549 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5506
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5506
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2595 2595
Size: 5506
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5506
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PspOMI G'GGCC_C
Cuts at: 2127 2235 2441 2127
Size: 108 206 5192
Fragments arranged by size:
5192 206 108
PstI C_TGCA'G
Cuts at: 830 2352 2858 830
Size: 1522 506 3478
Fragments arranged by size:
3478 1522 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
PvuII CAG'CTG
Cuts at: 2286 2485 2286
Size: 199 5307
SacII CC_GC'GG
Cuts at: 2581 2581
Size: 5506
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SapI GCTCTTCn'nnn_
Cuts at: 2088 2088
Size: 5506
SbfI CC_TGCA'GG
Cuts at: 2858 2858
Size: 5506
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2348 2854 3989 4670 4861 826
Size: 219 1303 506 1135 681 191 1471
Fragments arranged by size:
1471 1303 1135 681 506 219 191
SfoI GGC'GCC
Cuts at: 2138 2138
Size: 5506
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2240 2833 1872
Size: 368 593 4545
Fragments arranged by size:
4545 593 368
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3610 4511 4773 820
Size: 197 926 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2315 2864 1132
Size: 1183 549 3774
Fragments arranged by size:
3774 1183 549
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 2675 1517
Size: 1158 4348
StyI C'CwwG_G
Cuts at: 513 1065 2004 2945 513
Size: 552 939 941 3074
Fragments arranged by size:
3074 941 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3533 3550 3703 3888 4333 325
Size: 1255 1953 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3752 5464
Size: 274 80 33 51 1443 219 1556 1712
Cuts at: 5464 96
Size: 138
Fragments arranged by size:
1712 1556 1443 274 219 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5466 5498 5466
Size: 32 5474
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2228 2228
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5506
XmaI C'CCGG_G
Cuts at: 1870 2238 2831 1870
Size: 368 593 4545
Fragments arranged by size:
4545 593 368
XmnI GAAnn'nnTTC
Cuts at: 2734 3635 2734
Size: 901 4605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3314 276
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SrfI SwaI