Restriction map for
pFN21AE4043
(Circular) (Six-base) MAPSORT of: pFN21AE4043.seq Check: 1274 from: 1 to: 5503
pFN21AE4043 5503 bp
With 182 enzymes: SgfI *
March 28, 2012 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2437 2437
Size: 5503
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4950 5218 1632
Size: 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 268
AfeI AGC'GCT
Cuts at: 2108 2108
Size: 5503
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 2765 2797 2483
Size: 32 250 32 5189
Fragments arranged by size:
5189 250 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2068 2452 3246 4818 2068
Size: 384 794 1572 2753
Fragments arranged by size:
2753 1572 794 384
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2812 3038 3190 5299 1493
Size: 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 1870
Size: 73 885 4545
Fragments arranged by size:
4545 885 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2824 4279 618
Size: 325 217 627 1037 1455 1842
Fragments arranged by size:
1842 1455 1037 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2822 1391
Size: 1431 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 2722 928
Size: 971 471 352 3709
Fragments arranged by size:
3709 971 471 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3694 3728 1903
Size: 34 50 34 1673 34 3678
Fragments arranged by size:
3678 1673 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 2404 1548
Size: 856 4647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2571 5498 2571
Size: 2927 2576
BlpI GC'TnA_GC
Cuts at: 2920 2920
Size: 5503
Bme1580I G_kGCm'C
Cuts at: 1790 3564 4720 1790
Size: 1774 1156 2573
Fragments arranged by size:
2573 1774 1156
BmgBI CAC'GTC
Cuts at: 1318 2101 1318
Size: 783 4720
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 4192 442
Size: 416 53 83 399 24 1817 958 1753
Fragments arranged by size:
1817 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2546 4163 1239
Size: 806 501 1617 2579
Fragments arranged by size:
2579 1617 806 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2239 4166 882
Size: 617 740 1927 2219
Fragments arranged by size:
2219 1927 740 617
BsaAI yAC'GTr
Cuts at: 493 1204 3294 493
Size: 711 2090 2702
Fragments arranged by size:
2702 2090 711
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 3310 3692 4327
Size: 53 83 186 936 498 1279 382 635
Cuts at: 4327 275
Size: 1451
Fragments arranged by size:
1451 1279 936 635 498 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3936 4334 4608 4755 5166 3936
Size: 398 274 147 411 4273
Fragments arranged by size:
4273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2738 2768 1178
Size: 30 165 30 786 30 519 30 3913
Fragments arranged by size:
3913 786 519 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2238 2585 1186
Size: 1052 347 4104
Fragments arranged by size:
4104 1052 347
BseYI C'CCAG_C
Cuts at: 4706 5202 4706
Size: 496 5007
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 1163
Size: 573 756 4174
Fragments arranged by size:
4174 756 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2515 2953 3714 4742 1540
Size: 275 186 514 438 761 1028 2301
Fragments arranged by size:
2301 1028 761 514 438 275 186
BsiHKAI G_wGCw'C
Cuts at: 3564 3649 4720 3564
Size: 85 1071 4347
Fragments arranged by size:
4347 1071 85
BsmI GAATG_Cn'
Cuts at: 2677 3014 3107 2677
Size: 337 93 5073
Fragments arranged by size:
5073 337 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2543 2543
Size: 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2822 3564 3649 4720 1391
Size: 399 1032 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 742 399 85
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 2034 3392 5122 2034
Size: 1358 1730 2415
Fragments arranged by size:
2415 1730 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2437 2858 844
Size: 1593 421 3489
Fragments arranged by size:
3489 1593 421
BsrBI CCG'CTC
Cuts at: 1394 1823 3390 1394
Size: 429 1567 3507
Fragments arranged by size:
3507 1567 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 3998 4172 65
Size: 1183 901 1849 174 1396
Fragments arranged by size:
1849 1396 1183 901 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 2366 4147 4334 1700
Size: 110 239 317 1781 187 2869
Fragments arranged by size:
2869 1781 317 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 2714 3563 4575 2714
Size: 849 1012 3642
Fragments arranged by size:
3642 1012 849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2452 794
Size: 1658 3845
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2571 2718 2833 3200 3585 3602 5054 5140
Size: 599 147 115 367 385 17 1452 86
Cuts at: 5140 5152 5498 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 599 385 367 346 147 115
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2077 513
Size: 552 1012 3939
Fragments arranged by size:
3939 1012 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 2119 2692 3019 3813 3833 2119
Size: 573 327 794 20 3789
Fragments arranged by size:
3789 794 573 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2456 2491 529
Size: 35 1892 35 3541
Fragments arranged by size:
3541 1892 35 35
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 809
Size: 507 3194 1802
Fragments arranged by size:
3194 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2512 2652 2950
Size: 54 1425 50 275 18 682 140 298
Cuts at: 2950 3839 5468 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 682 298 275 140 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2512 2950 1537
Size: 275 700 438 4090
Fragments arranged by size:
4090 700 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3433 1353
Size: 2080 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2546 3566 4163 4950 5218 1239
Size: 393 413 501 1020 597 787 268 1524
Fragments arranged by size:
1524 1020 787 597 501 413 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2600 2600
Size: 5503
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 2056 2110 4650 1678
Size: 378 54 2540 2531
Fragments arranged by size:
2540 2531 378 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2738 2770 4144 4176 4218
Size: 32 135 32 1166 32 1374 32 42
Cuts at: 4218 4250 1373
Size: 32 2626
Fragments arranged by size:
2626 1374 1166 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 3093 669
Size: 988 281 909 246 3079
Fragments arranged by size:
3079 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 4617 4801 1305
Size: 837 2475 184 2007
Fragments arranged by size:
2475 2007 837 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 10
Size: 54 1425 343 822 2859
Fragments arranged by size:
2859 1425 822 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3461 3820 3979 518
Size: 648 2295 359 159 2042
Fragments arranged by size:
2295 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2919 3596 4744 4989 1606
Size: 1313 677 1148 245 2120
Fragments arranged by size:
2120 1313 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2077 513
Size: 552 1012 3939
Fragments arranged by size:
3939 1012 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2804 2861 4406 1132
Size: 97 1575 57 1545 2229
Fragments arranged by size:
2229 1575 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PfoI T'CCnGG_A
Cuts at: 2523 2544 2523
Size: 21 5482
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2042 1613
Size: 429 5074
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2457 2855 830
Size: 1627 398 3478
Fragments arranged by size:
3478 1627 398
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 2660 3751 5463 2660
Size: 1091 1712 2700
Fragments arranged by size:
2700 1712 1091
SexAI A'CCwGG_T
Cuts at: 2397 2397
Size: 5503
SfcI C'TryA_G
Cuts at: 826 1045 2453 2851 3986 4667 4858 826
Size: 219 1408 398 1135 681 191 1471
Fragments arranged by size:
1471 1408 1135 681 398 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 2049 1700
Size: 349 5154
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3607 4508 4770 820
Size: 197 926 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2077 2942 513
Size: 552 1012 865 3074
Fragments arranged by size:
3074 1012 865 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3530 3547 3700 3885 4330 325
Size: 1255 1950 17 153 185 445 1498
Fragments arranged by size:
1950 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2658 3749 5461
Size: 274 80 33 51 1443 681 1091 1712
Cuts at: 5461 96
Size: 138
Fragments arranged by size:
1712 1443 1091 681 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2527 2257
Size: 270 5233
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI