Restriction map for
pFN21AE4041
(Circular) (Six-base) MAPSORT of: pFN21AE4041.seq Check: 6378 from: 1 to: 5494
pFN21AE4041 5494 bp
With 182 enzymes: SgfI *
March 28, 2012 13:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2176 2815 2176
Size: 639 4855
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3557 4941 5209 1632
Size: 1925 1384 268 1917
Fragments arranged by size:
1925 1917 1384 268
AfeI AGC'GCT
Cuts at: 2353 2353
Size: 5494
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 2086 2088 4393 5370 1203
Size: 883 2 2305 977 1327
Fragments arranged by size:
2305 1327 977 883 2
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5494
AlwNI CAG_nnn'CTG
Cuts at: 2312 3237 4809 2312
Size: 925 1572 2997
Fragments arranged by size:
2997 1572 925
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2595 2628 2803 3029 3181 5290 1493
Size: 1102 33 175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1102 226 175 152 33
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 1870
Size: 73 876 4545
Fragments arranged by size:
4545 876 73
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2176 2815 4270 618
Size: 325 217 627 389 639 1455 1842
Fragments arranged by size:
1842 1455 639 627 389 325 217
BanII G_rGCy'C
Cuts at: 1391 2274 2751 2813 1391
Size: 883 477 62 4072
Fragments arranged by size:
4072 883 477 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 928
Size: 971 424 4099
Fragments arranged by size:
4099 971 424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3685 3719 1903
Size: 34 1748 34 3678
Fragments arranged by size:
3678 1748 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4105 136
Size: 107 122 71 631 605 2433 1525
Fragments arranged by size:
2433 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2911 2911
Size: 5494
Bme1580I G_kGCm'C
Cuts at: 1790 2153 3555 4711 1790
Size: 363 1402 1156 2573
Fragments arranged by size:
2573 1402 1156 363
BmgBI CAC'GTC
Cuts at: 1318 2167 1318
Size: 849 4645
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2455 2786 3225
Size: 416 53 83 399 24 1038 331 439
Cuts at: 3225 4183 442
Size: 958 1753
Fragments arranged by size:
1753 1038 958 439 416 399 331 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 4154 1239
Size: 1370 1545 2579
Fragments arranged by size:
2579 1545 1370
Bpu10I CC'TnA_GC
Cuts at: 2532 2532
Size: 5494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2765 3619 4484 4782 2264
Size: 501 854 865 298 2976
Fragments arranged by size:
2976 865 854 501 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 882
Size: 617 2658 2219
Fragments arranged by size:
2658 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2089 2260 3285 493
Size: 711 885 171 1025 2702
Fragments arranged by size:
2702 1025 885 711 171
BsaBI GATnn'nnATC
Cuts at: 5488 5488
Size: 5494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2487 3301 3683 4318
Size: 53 83 186 936 954 814 382 635
Cuts at: 4318 275
Size: 1451
Fragments arranged by size:
1451 954 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3927 4325 4599 4746 5157 3927
Size: 398 274 147 411 4264
Fragments arranged by size:
4264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2711 2741 1178
Size: 30 165 30 1308 30 3931
Fragments arranged by size:
3931 1308 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2419 1186
Size: 1096 137 4261
Fragments arranged by size:
4261 1096 137
BseYI C'CCAG_C
Cuts at: 4697 5193 4697
Size: 496 4998
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2240 1163
Size: 573 504 4417
Fragments arranged by size:
4417 573 504
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 2751 3555 3640 4711 2274
Size: 477 804 85 1071 3057
Fragments arranged by size:
3057 1071 804 477 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
BsmBI CGTCTCn'nnnn_
Cuts at: 2157 2157
Size: 5494
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2274 2751 2813 3555 3640 4711
Size: 399 363 121 477 62 742 85 1071
Cuts at: 4711 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 477 399 363 121 85
62
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2188 2849 844
Size: 1344 661 3489
Fragments arranged by size:
3489 1344 661
BsrBI CCG'CTC
Cuts at: 1394 1823 3381 1394
Size: 429 1558 3507
Fragments arranged by size:
3507 1558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2524 3989 4163 65
Size: 1183 1276 1465 174 1396
Fragments arranged by size:
1465 1396 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2500 4138 4325 1700
Size: 110 690 1638 187 2869
Fragments arranged by size:
2869 1638 690 187 110
BsrGI T'GTAC_A
Cuts at: 96 2081 96
Size: 1985 3509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2254 2312 2573 794
Size: 1460 58 261 3715
Fragments arranged by size:
3715 1460 261 58
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2367 2389 2824 3191 3576 3593 5045 5131
Size: 395 22 435 367 385 17 1452 86
Cuts at: 5131 5143 5489 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 435 395 385 367 346 86
22 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4003
Fragments arranged by size:
4003 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2159 487
Size: 683 56 933 3822
Fragments arranged by size:
3822 933 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 3010 3804 3824 2310
Size: 700 794 20 3980
Fragments arranged by size:
3980 794 700 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2681 4501 809
Size: 507 1365 1820 1802
Fragments arranged by size:
1820 1802 1365 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2434 2941
Size: 54 1425 50 275 18 177 427 507
Cuts at: 2941 3830 5459 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 507 427 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3424 1353
Size: 2071 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4081 4455 4601 387
Size: 1064 138 2492 374 146 1280
Fragments arranged by size:
2492 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2272 2749 2272
Size: 477 5017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 3557 4154 4941 5209 1239
Size: 393 977 948 597 787 268 1524
Fragments arranged by size:
1524 977 948 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2595 2803 3181 2595
Size: 208 378 4908
Fragments arranged by size:
4908 378 208
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 2355 4641 1678
Size: 677 2286 2531
Fragments arranged by size:
2531 2286 677
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2374 2406 2456
Size: 32 135 32 470 32 300 32 50
Cuts at: 2456 2488 4135 4167 4209 4241 1373
Size: 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 470 300 135 50 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 2838 3084 669
Size: 988 281 736 164 246 3079
Fragments arranged by size:
3079 988 736 281 246 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2180 2819 2180
Size: 639 4855
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4608 4792 1305
Size: 3303 184 2007
Fragments arranged by size:
3303 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3495
Fragments arranged by size:
3495 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3452 3811 3970 518
Size: 648 2286 359 159 2042
Fragments arranged by size:
2286 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2910 3587 4735 4980 1606
Size: 1304 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 677 245
NaeI GCC'GGC
Cuts at: 2502 2502
Size: 5494
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4003
Fragments arranged by size:
4003 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NgoMIV G'CCGG_C
Cuts at: 2500 2500
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2852 4397 1132
Size: 97 1623 1545 2229
Fragments arranged by size:
2229 1623 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2482 1348
Size: 1134 4360
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PfoI T'CCnGG_A
Cuts at: 2634 2634
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PmlI CAC'GTG
Cuts at: 2089 2260 2089
Size: 171 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2846 830
Size: 2016 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
SacI G_AGCT'C
Cuts at: 2274 2751 2274
Size: 477 5017
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2842 3977 4658 4849 826
Size: 219 1797 1135 681 191 1471
Fragments arranged by size:
1797 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2744 3598 4499 4761 820
Size: 197 926 300 501 854 901 262 1553
Fragments arranged by size:
1553 926 901 854 501 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5494
StyI C'CwwG_G
Cuts at: 513 1065 2004 2933 513
Size: 552 939 929 3074
Fragments arranged by size:
3074 939 929 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 3521 3538 3691 3876 4321 325
Size: 1255 1017 924 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1017 924 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2081 3740 5452
Size: 274 80 33 51 1443 104 1659 1712
Cuts at: 5452 96
Size: 138
Fragments arranged by size:
1712 1659 1443 274 138 104 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2711 2743 5454 5486 2711
Size: 32 2711 32 2719
Fragments arranged by size:
2719 2711 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI