Restriction map for
pFN21AE4040
(Circular) (Six-base) MAPSORT of: pFN21AE4040.seq Check: 2398 from: 1 to: 5494
pFN21AE4040 5494 bp
With 182 enzymes: SgfI *
March 28, 2012 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 3557 4941 5209 1632
Size: 1124 801 1384 268 1917
Fragments arranged by size:
1917 1384 1124 801 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 2559 4393 5370 1203
Size: 1356 1834 977 1327
Fragments arranged by size:
1834 1356 1327 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5494
AlwNI CAG_nnn'CTG
Cuts at: 2167 3237 4809 2167
Size: 1070 1572 2852
Fragments arranged by size:
2852 1572 1070
ApaI G_GGCC'C
Cuts at: 2097 2097
Size: 5494
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2630 2690 2803 3029 3181 5290 1493
Size: 1137 60 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1137 226 152 113 60
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 2819 1870
Size: 73 246 630 4545
Fragments arranged by size:
4545 630 246 73
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2243 2815 4270 618
Size: 325 217 627 456 572 1455 1842
Fragments arranged by size:
1842 1455 627 572 456 325 217
BanII G_rGCy'C
Cuts at: 1391 2097 2286 2595 2813 1391
Size: 706 189 309 218 4072
Fragments arranged by size:
4072 706 309 218 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2233 2267 3685 3719 1903
Size: 34 296 34 1418 34 3678
Fragments arranged by size:
3678 1418 296 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3386 4596 2508
Size: 878 1210 3406
Fragments arranged by size:
3406 1210 878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2115 2131 4105
Size: 107 122 71 631 605 443 16 1974
Cuts at: 4105 136
Size: 1525
Fragments arranged by size:
1974 1525 631 605 443 122 107 71
16
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2911 2911
Size: 5494
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2136 3555 4711 1790
Size: 307 39 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 307 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 3225 4183
Size: 416 53 83 399 24 661 1147 958
Cuts at: 4183 442
Size: 1753
Fragments arranged by size:
1753 1147 958 661 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2480 2692 4154 1239
Size: 950 291 212 1462 2579
Fragments arranged by size:
2579 1462 950 291 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 882
Size: 617 2658 2219
Fragments arranged by size:
2658 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 3285 493
Size: 711 1356 725 2702
Fragments arranged by size:
2702 1356 725 711
BsaBI GATnn'nnATC
Cuts at: 5488 5488
Size: 5494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3301 3683 4318 275
Size: 53 83 186 936 1768 382 635 1451
Fragments arranged by size:
1768 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3927 4325 4599 4746 5157 3927
Size: 398 274 147 411 4264
Fragments arranged by size:
4264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 1178
Size: 30 165 30 870 30 4369
Fragments arranged by size:
4369 870 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5494
BseYI C'CCAG_C
Cuts at: 2041 2099 2220 4697 5193 2041
Size: 58 121 2477 496 2342
Fragments arranged by size:
2477 2342 496 121 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4921
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 2944 3705 4733 1540
Size: 275 186 139 804 761 1028 2301
Fragments arranged by size:
2301 1028 804 761 275 186 139
BsiHKAI G_wGCw'C
Cuts at: 2595 3555 3640 4711 2595
Size: 960 85 1071 3378
Fragments arranged by size:
3378 1071 960 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2136 2286 2595 2813 3555 3640
Size: 399 307 39 150 309 218 742 85
Cuts at: 3640 4711 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 309 307 218 150
85 39
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2849 844
Size: 2005 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2194 3381 1394
Size: 429 371 1187 3507
Fragments arranged by size:
3507 1187 429 371
BsrDI GCAATG_nn'
Cuts at: 65 1248 2680 3989 4163 65
Size: 1183 1432 1309 174 1396
Fragments arranged by size:
1432 1396 1309 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4138 4325 1700
Size: 110 2328 187 2869
Fragments arranged by size:
2869 2328 187 110
BsrGI T'GTAC_A
Cuts at: 96 2562 96
Size: 2466 3028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5494
BstBI TT'CG_AA
Cuts at: 2634 4298 2634
Size: 1664 3830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2654 2824 3191 3576 3593 5045 5131 5143
Size: 682 170 367 385 17 1452 86 12
Cuts at: 5143 5489 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 682 385 367 346 170 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2272 2272
Size: 5494
BtgI C'CryG_G
Cuts at: 513 1065 2004 2089 2128 2413 513
Size: 552 939 85 39 285 3594
Fragments arranged by size:
3594 939 552 285 85 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2045 2467 487
Size: 683 56 819 422 3514
Fragments arranged by size:
3514 819 683 422 56
BtsI GCAGTG_nn'
Cuts at: 2582 3010 3804 3824 2582
Size: 428 794 20 4252
Fragments arranged by size:
4252 794 428 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2368 2403 529
Size: 35 1804 35 3620
Fragments arranged by size:
3620 1804 35 35
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2941 3830
Size: 54 1425 50 275 18 307 804 889
Cuts at: 3830 5459 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 804 307 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2137 2941 1537
Size: 275 325 804 4090
Fragments arranged by size:
4090 804 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2583 3424 1353
Size: 1230 841 3423
Fragments arranged by size:
3423 1230 841
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2399 4081 4455 4601 387
Size: 1064 138 435 375 1682 374 146 1280
Fragments arranged by size:
1682 1280 1064 435 375 374 146 138
EcoICRI GAG'CTC
Cuts at: 2593 2593
Size: 5494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2480 2692 2756 3557 4154 4941
Size: 393 557 291 212 64 801 597 787
Cuts at: 4941 5209 1239
Size: 268 1524
Fragments arranged by size:
1524 801 787 597 557 393 291 268
212 64
EcoO109I rG'GnC_Cy
Cuts at: 2094 2177 2320 2374 2546 2094
Size: 83 143 54 172 5042
Fragments arranged by size:
5042 172 143 83 54
EcoRI G'AATT_C
Cuts at: 2690 2803 3181 2690
Size: 113 378 5003
Fragments arranged by size:
5003 378 113
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 4641 1678
Size: 2963 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2768 2800 4135
Size: 32 135 32 701 32 463 32 1335
Cuts at: 4135 4167 4209 4241 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1335 701 463 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2643 4608 4792 1305
Size: 1338 1965 184 2007
Fragments arranged by size:
2007 1965 1338 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3672
Fragments arranged by size:
3672 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 3452 3811 3970 518
Size: 648 1434 852 359 159 2042
Fragments arranged by size:
2042 1434 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2091 2130 2167 2220 2910 3587 4735
Size: 414 71 39 37 53 690 677 1148
Cuts at: 4735 4980 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 690 677 414 245 71 53
39 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4003
Fragments arranged by size:
4003 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2852 4397 1132
Size: 97 1623 1545 2229
Fragments arranged by size:
2229 1623 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PfoI T'CCnGG_A
Cuts at: 2179 2179
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PmlI CAC'GTG
Cuts at: 2560 2560
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2177 2320 2374 2177
Size: 143 54 5297
Fragments arranged by size:
5297 143 54
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PspOMI G'GGCC_C
Cuts at: 2093 2093
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2019 2577 2846 830
Size: 1189 558 269 3478
Fragments arranged by size:
3478 1189 558 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
PvuII CAG'CTG
Cuts at: 2020 2020
Size: 5494
SacI G_AGCT'C
Cuts at: 2595 2595
Size: 5494
SacII CC_GC'GG
Cuts at: 2092 2131 2092
Size: 39 5455
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SanDI GG'GwC_CC
Cuts at: 2177 2374 2177
Size: 197 5297
SapI GCTCTTCn'nnn_
Cuts at: 2583 2583
Size: 5494
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2015 2573 2842 3977 4658 4849 826
Size: 219 970 558 269 1135 681 191 1471
Fragments arranged by size:
1471 1135 970 681 558 269 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3598 4499 4761 820
Size: 197 926 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5494
StyI C'CwwG_G
Cuts at: 513 1065 2004 2933 513
Size: 552 939 929 3074
Fragments arranged by size:
3074 939 929 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 3740 5452
Size: 274 80 33 51 1443 585 1178 1712
Cuts at: 5452 96
Size: 138
Fragments arranged by size:
1712 1443 1178 585 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5454 5486 5454
Size: 32 5462
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI
SfoI SrfI SwaI XcmI