Restriction map for
pFN21AE4037
(Circular) (Six-base) MAPSORT of: pFN21AE4037.seq Check: 4220 from: 1 to: 6268
pFN21AE4037 6268 bp
With 182 enzymes: SgfI *
March 28, 2012 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2968 2968
Size: 6268
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2543 3611 1937
Size: 606 1068 4594
Fragments arranged by size:
4594 1068 606
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 2710 3375 4331 5715 5983 1632
Size: 870 208 665 956 1384 268 1917
Fragments arranged by size:
1917 1384 956 870 665 268 208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 3306 5167 6144 1203
Size: 2103 1861 977 1327
Fragments arranged by size:
2103 1861 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AlwNI CAG_nnn'CTG
Cuts at: 2510 2887 3134 4011 5583 2510
Size: 377 247 877 1572 3195
Fragments arranged by size:
3195 1572 877 377 247
ApaI G_GGCC'C
Cuts at: 2704 2704
Size: 6268
ApaLI G'TGCA_C
Cuts at: 2159 4325 5481 2159
Size: 2166 1156 2946
Fragments arranged by size:
2946 2166 1156
ApoI r'AATT_y
Cuts at: 1493 3275 3538 3577 3803 3955 6064 1493
Size: 1782 263 39 226 152 2109 1697
Fragments arranged by size:
2109 1782 1697 263 226 152 39
AseI AT'TA_AT
Cuts at: 160 1032 4822 160
Size: 872 3790 1606
Fragments arranged by size:
3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 3251 3593 1870
Size: 73 1308 342 4545
Fragments arranged by size:
4545 1308 342 73
AvrII C'CTAG_G
Cuts at: 2695 2695
Size: 6268
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2600 2681 3589 5044
Size: 325 217 627 306 507 81 908 1455
Cuts at: 5044 618
Size: 1842
Fragments arranged by size:
1842 1455 908 627 507 325 306 217
81
BanII G_rGCy'C
Cuts at: 1391 2704 2847 3245 3421 3587 1391
Size: 1313 143 398 176 166 4072
Fragments arranged by size:
4072 1313 398 176 166 143
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 6268
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5297
BbvCI CC'TCA_GC
Cuts at: 2234 2234
Size: 6268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4459 4493 1903
Size: 34 2522 34 3678
Fragments arranged by size:
3678 2522 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2947 3118 4160 5370 2947
Size: 171 1042 1210 3845
Fragments arranged by size:
3845 1210 1042 171
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4879 136
Size: 107 122 71 631 605 337 2870 1525
Fragments arranged by size:
2870 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 3685 3685
Size: 6268
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2704 4329 5485 1790
Size: 373 541 1625 1156 2573
Fragments arranged by size:
2573 1625 1156 541 373
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2993 3469 3999
Size: 416 53 83 399 24 1576 476 530
Cuts at: 3999 4957 442
Size: 958 1753
Fragments arranged by size:
1753 1576 958 530 476 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2831 4928 1239
Size: 1592 2097 2579
Fragments arranged by size:
2579 2097 1592
Bpu10I CC'TnA_GC
Cuts at: 2234 2748 3244 2234
Size: 514 496 5258
Fragments arranged by size:
5258 514 496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3169 4393 5258 5556 3169
Size: 1224 865 298 3881
Fragments arranged by size:
3881 1224 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3354 4931 882
Size: 617 1855 1577 2219
Fragments arranged by size:
2219 1855 1577 617
BsaAI yAC'GTr
Cuts at: 493 1204 2124 4059 493
Size: 711 920 1935 2702
Fragments arranged by size:
2702 1935 920 711
BsaBI GATnn'nnATC
Cuts at: 6262 6262
Size: 6268
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2175 4075 4457
Size: 53 83 186 936 561 81 1900 382
Cuts at: 4457 5092 275
Size: 635 1451
Fragments arranged by size:
1900 1451 936 635 561 382 186 83
81 53
BsaWI w'CCGG_w
Cuts at: 3219 4701 5099 5373 5520 5931 3219
Size: 1482 398 274 147 411 3556
Fragments arranged by size:
3556 1482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6043
Fragments arranged by size:
6043 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 3173 1186
Size: 1633 354 4281
Fragments arranged by size:
4281 1633 354
BseYI C'CCAG_C
Cuts at: 2365 2526 2677 3120 3503 5471 5967 2365
Size: 161 151 443 383 1968 496 2666
Fragments arranged by size:
2666 1968 496 443 383 161 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5695
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 2847 3245 3421 4329 4414 5485 2163
Size: 684 398 176 908 85 1071 2946
Fragments arranged by size:
2946 1071 908 684 398 176 85
BsiWI C'GTAC_G
Cuts at: 2124 2124
Size: 6268
BsmI GAATG_Cn'
Cuts at: 2263 3779 3872 2263
Size: 1516 93 4659
Fragments arranged by size:
4659 1516 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2704 2847 3245 3421 3587 4329
Size: 399 373 541 143 398 176 166 742
Cuts at: 4329 4414 5485 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 541 399 398 373 176
166 143 85
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 4157 5887 4157
Size: 1730 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 2601 2968 3623 844
Size: 1392 365 367 655 3489
Fragments arranged by size:
3489 1392 655 367 365
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2740 4155 1394
Size: 429 343 574 1415 3507
Fragments arranged by size:
3507 1415 574 429 343
BsrDI GCAATG_nn'
Cuts at: 65 1248 3468 4763 4937 65
Size: 1183 2220 1295 174 1396
Fragments arranged by size:
2220 1396 1295 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4912 5099 1700
Size: 110 3102 187 2869
Fragments arranged by size:
3102 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2786 794
Size: 1992 4276
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 2830 2206
Size: 624 5644
BstYI r'GATC_y
Cuts at: 1972 2355 3598 3965 4350 4367 5819 5905 5917
Size: 383 1243 367 385 17 1452 86 12
Cuts at: 5917 6263 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1243 385 383 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3097 3097
Size: 6268
BtgI C'CryG_G
Cuts at: 513 1065 2004 2811 513
Size: 552 939 807 3970
Fragments arranged by size:
3970 939 807 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 2560 3382 3784 4578 4598 2560
Size: 822 402 794 20 4230
Fragments arranged by size:
4230 822 794 402 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DraIII CAC_nnn'GTG
Cuts at: 2962 2962
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3522 3715 4604
Size: 54 1425 50 275 18 1692 193 889
Cuts at: 4604 6233 8
Size: 1629 43
Fragments arranged by size:
1692 1629 1425 889 275 193 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 4198 1353
Size: 2041 804 3423
Fragments arranged by size:
3423 2041 804
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 2816 4855 5229 5375 387
Size: 1064 138 438 789 2039 374 146 1280
Fragments arranged by size:
2039 1280 1064 789 438 374 146 138
EcoICRI GAG'CTC
Cuts at: 2845 3243 3419 2845
Size: 398 176 5694
Fragments arranged by size:
5694 398 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 2710 2831 3375 4331 4928 5715
Size: 393 870 208 121 544 956 597 787
Cuts at: 5715 5983 1239
Size: 268 1524
Fragments arranged by size:
1524 956 870 787 597 544 393 268
208 121
EcoO109I rG'GnC_Cy
Cuts at: 2523 2700 2523
Size: 177 6091
EcoRI G'AATT_C
Cuts at: 3538 3577 3955 3538
Size: 39 378 5851
Fragments arranged by size:
5851 378 39
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 2097 5415 1678
Size: 419 3318 2531
Fragments arranged by size:
3318 2531 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3332 3364 4909 4941 4983
Size: 32 135 32 1760 32 1545 32 42
Cuts at: 4983 5015 1373
Size: 32 2626
Fragments arranged by size:
2626 1760 1545 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3612 3858 669
Size: 988 281 1674 246 3079
Fragments arranged by size:
3079 1674 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3106 748
Size: 2358 3910
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 6268
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 3867 3867
Size: 6268
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2090 2187 2238 5382 5566 1305
Size: 785 97 51 3144 184 2007
Fragments arranged by size:
3144 2007 785 184 97 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3524 10
Size: 54 1425 343 1692 2754
Fragments arranged by size:
2754 1692 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4226 4585 4744 518
Size: 648 3060 359 159 2042
Fragments arranged by size:
3060 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2365 3028 3120 3464 3684 4361 5509
Size: 627 132 663 92 344 220 677 1148
Cuts at: 5509 5754 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 663 627 344 245 220
132 92
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 6268
NcoI C'CATG_G
Cuts at: 513 1065 2004 2811 513
Size: 552 939 807 3970
Fragments arranged by size:
3970 939 807 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 3310 3626 5171 1132
Size: 97 2081 316 1545 2229
Fragments arranged by size:
2229 2081 1545 316 97
PasI CC'CwG_GG
Cuts at: 1348 2170 1348
Size: 822 5446
PciI A'CATG_T
Cuts at: 3306 5167 3306
Size: 1861 4407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2523 2523
Size: 6268
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PspOMI G'GGCC_C
Cuts at: 2700 2700
Size: 6268
PspXI vC'TCGA_Gb
Cuts at: 3251 3251
Size: 6268
PstI C_TGCA'G
Cuts at: 830 3360 3620 830
Size: 2530 260 3478
Fragments arranged by size:
3478 2530 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
PvuII CAG'CTG
Cuts at: 2233 2365 3028 2233
Size: 132 663 5473
Fragments arranged by size:
5473 663 132
SacI G_AGCT'C
Cuts at: 2847 3245 3421 2847
Size: 398 176 5694
Fragments arranged by size:
5694 398 176
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SanDI GG'GwC_CC
Cuts at: 2523 2523
Size: 6268
SapI GCTCTTCn'nnn_
Cuts at: 3394 3394
Size: 6268
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 2544 2879 3356 3459 3616 4751 5432
Size: 219 1499 335 477 103 157 1135 681
Cuts at: 5432 5623 826
Size: 191 1471
Fragments arranged by size:
1499 1471 1135 681 477 335 219 191
157 103
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 6268
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3184 3251 4372 5273 5535 820
Size: 197 926 1241 67 1121 901 262 1553
Fragments arranged by size:
1553 1241 1121 926 901 262 197 67
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3626 1132
Size: 2494 3774
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 2078 2694 3249 1517
Size: 561 616 555 4536
Fragments arranged by size:
4536 616 561 555
StyI C'CwwG_G
Cuts at: 513 1065 2004 2695 2811 3707 513
Size: 552 939 691 116 896 3074
Fragments arranged by size:
3074 939 896 691 552 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 2623 2669 4295 4312 4465 4650
Size: 1255 462 581 46 1626 17 153 185
Cuts at: 4650 5095 325
Size: 445 1498
Fragments arranged by size:
1626 1498 1255 581 462 445 185 153
46 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3075 4514 6226
Size: 274 80 33 51 1443 1098 1439 1712
Cuts at: 6226 96
Size: 138
Fragments arranged by size:
1712 1443 1439 1098 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6228 6260 6228
Size: 32 6236
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6268
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2617 2617
Size: 6268
XhoI C'TCGA_G
Cuts at: 1943 3251 1943
Size: 1308 4960
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 2225 4397 2225
Size: 2172 4096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BplI BsmBI BstZ17I
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PsrI RsrII SacII SexAI
SfiI SrfI SwaI