Restriction map for
pFN21AE4034
(Circular) (Six-base) MAPSORT of: pFN21AE4034.seq Check: 5130 from: 1 to: 5437
pFN21AE4034 5437 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2614 2780 1937
Size: 677 166 4594
Fragments arranged by size:
4594 677 166
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3500 4884 5152 1632
Size: 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 4321
AlwNI CAG_nnn'CTG
Cuts at: 2407 2506 2569 3180 4752 2407
Size: 99 63 611 1572 3092
Fragments arranged by size:
3092 1572 611 99 63
ApaI G_GGCC'C
Cuts at: 2027 2497 2027
Size: 470 4967
ApaLI G'TGCA_C
Cuts at: 2702 3494 4650 2702
Size: 792 1156 3489
Fragments arranged by size:
3489 1156 792
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 2189 2762 1870
Size: 73 225 21 573 4545
Fragments arranged by size:
4545 573 225 73 21
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2048 2696 2758 4213
Size: 325 217 627 221 40 648 62 1455
Cuts at: 4213 618
Size: 1842
Fragments arranged by size:
1842 1455 648 627 325 221 217 62
40
BanII G_rGCy'C
Cuts at: 1391 2027 2069 2497 2730 2756 1391
Size: 636 42 428 233 26 4072
Fragments arranged by size:
4072 636 428 233 42 26
BbeI G_GCGC'C
Cuts at: 2012 2052 2012
Size: 40 5397
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2096 928
Size: 971 197 4269
Fragments arranged by size:
4269 971 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2497 2706 3498 4654 1790
Size: 237 470 209 792 1156 2573
Fragments arranged by size:
2573 1156 792 470 237 209
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4126 442
Size: 416 53 83 399 24 1751 958 1753
Fragments arranged by size:
1753 1751 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 4097 1239
Size: 1001 1857 2579
Fragments arranged by size:
2579 1857 1001
Bpu10I CC'TnA_GC
Cuts at: 2570 2570
Size: 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2049 3244 3626
Size: 53 83 186 936 476 40 1195 382
Cuts at: 3626 4261 275
Size: 635 1451
Fragments arranged by size:
1451 1195 936 635 476 382 186 83
53 40
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2222 2237 2720 1186
Size: 1027 9 15 483 3903
Fragments arranged by size:
3903 1027 483 15 9
BseYI C'CCAG_C
Cuts at: 2020 2041 2062 2121 2251 2608 4640 5136 2020
Size: 21 21 59 130 357 2032 496 2321
Fragments arranged by size:
2321 2032 496 357 130 59 21 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 1163
Size: 573 756 4108
Fragments arranged by size:
4108 756 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 2104 2110 2887 3648 4676
Size: 275 186 17 86 6 777 761 1028
Cuts at: 4676 1540
Size: 2301
Fragments arranged by size:
2301 1028 777 761 275 186 86 17
6
BsiHKAI G_wGCw'C
Cuts at: 2076 2279 2706 2730 3498 3583 4654 2076
Size: 203 427 24 768 85 1071 2859
Fragments arranged by size:
2859 1071 768 427 203 85 24
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2069 2076 2279 2497 2706 2730
Size: 399 237 42 7 203 218 209 24
Cuts at: 2730 2756 3498 3583 4654 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 237 218 209 203
85 42 26 24 7
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2415 2544 2792 844
Size: 1544 27 129 248 3489
Fragments arranged by size:
3489 1544 248 129 27
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 3324 1394
Size: 429 293 1208 3507
Fragments arranged by size:
3507 1208 429 293
BsrDI GCAATG_nn'
Cuts at: 65 1248 2661 3932 4106 65
Size: 1183 1413 1271 174 1396
Fragments arranged by size:
1413 1396 1271 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 4081 4268 1700
Size: 110 402 1869 187 2869
Fragments arranged by size:
2869 1869 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 794
Size: 1721 3716
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5437
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 2587
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2767 3134 3519 3536 4988 5074 5086 5432
Size: 795 367 385 17 1452 86 12 346
Cuts at: 5432 1972
Size: 1977
Fragments arranged by size:
1977 1452 795 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2052 2104 513
Size: 552 939 8 40 52 3846
Fragments arranged by size:
3846 939 552 52 40 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 2625 1554
Size: 1071 4366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2540 4444 809
Size: 507 1224 1904 1802
Fragments arranged by size:
1904 1802 1224 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2101 2107
Size: 54 1425 50 275 18 185 86 6
Cuts at: 2107 2553 2884 3773 5402 8
Size: 446 331 889 1629 43
Fragments arranged by size:
1629 1425 889 446 331 275 185 86
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2015 2101 2107 2884 1537
Size: 275 203 86 6 777 4090
Fragments arranged by size:
4090 777 275 203 86 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2682 3367 1353
Size: 728 601 685 3423
Fragments arranged by size:
3423 728 685 601
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2728 2728
Size: 5437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 3500 4097 4884 5152 1239
Size: 393 608 1260 597 787 268 1524
Fragments arranged by size:
1524 1260 787 608 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 5437
EcoO109I rG'GnC_Cy
Cuts at: 2024 2185 2527 2566 2675 2024
Size: 161 342 39 109 4786
Fragments arranged by size:
4786 342 161 109 39
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 5437
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 2012 2042 2052 2122 2219 4584 1678
Size: 334 30 10 70 97 2365 2531
Fragments arranged by size:
2531 2365 334 97 70 30 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4078 4110 4152 4184 1373
Size: 32 135 32 2506 32 42 32 2626
Fragments arranged by size:
2626 2506 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KasI G'GCGC_C
Cuts at: 2008 2048 2008
Size: 40 5397
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4551 4735 1305
Size: 3246 184 2007
Fragments arranged by size:
3246 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 10
Size: 54 1425 343 723 2892
Fragments arranged by size:
2892 1425 723 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 3395 3754 3913 518
Size: 648 1419 810 359 159 2042
Fragments arranged by size:
2042 1419 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2020 2054 2106 2311 2602 2635 2853
Size: 408 6 34 52 205 291 33 218
Cuts at: 2853 3530 4678 4923 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 408 291 245 218 205
52 34 33 6
NaeI GCC'GGC
Cuts at: 2214 2214
Size: 5437
NarI GG'CG_CC
Cuts at: 2009 2049 2009
Size: 40 5397
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NgoMIV G'CCGG_C
Cuts at: 2212 2212
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2015 2884 2015
Size: 869 4568
NspI r_CATG'y
Cuts at: 1132 1229 2795 4340 1132
Size: 97 1566 1545 2229
Fragments arranged by size:
2229 1566 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2490 1348
Size: 1142 4295
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2185 2527 2566 2675 2185
Size: 342 39 109 4947
Fragments arranged by size:
4947 342 109 39
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PspOMI G'GGCC_C
Cuts at: 2023 2493 2023
Size: 470 4967
PstI C_TGCA'G
Cuts at: 830 2601 2674 2789 830
Size: 1771 73 115 3478
Fragments arranged by size:
3478 1771 115 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
SacI G_AGCT'C
Cuts at: 2730 2730
Size: 5437
SacII CC_GC'GG
Cuts at: 2015 2055 2107 2015
Size: 40 52 5345
Fragments arranged by size:
5345 52 40
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SanDI GG'GwC_CC
Cuts at: 2185 2185
Size: 5437
SapI GCTCTTCn'nnn_
Cuts at: 2081 2682 2081
Size: 601 4836
SbfI CC_TGCA'GG
Cuts at: 2674 2789 2674
Size: 115 5322
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2597 2615 2670 2785 3920 4601 4792
Size: 219 1552 18 55 115 1135 681 191
Cuts at: 4792 826
Size: 1471
Fragments arranged by size:
1552 1471 1135 681 219 191 115 55
18
SfoI GGC'GCC
Cuts at: 2010 2050 2010
Size: 40 5397
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2764 1872
Size: 892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 2138 1517
Size: 621 4816
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2361 2876 513
Size: 552 939 24 333 515 3074
Fragments arranged by size:
3074 939 552 515 333 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2490 3464 3481 3634 3819 4264 325
Size: 1255 910 974 17 153 185 445 1498
Fragments arranged by size:
1498 1255 974 910 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2762 1870
Size: 892 4545
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SexAI SfiI SrfI SwaI XcmI