Restriction map for
pFN21AE4032
(Circular) (Six-base) MAPSORT of: pFN21AE4032.seq Check: 9345 from: 1 to: 5431
pFN21AE4032 5431 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2290 2709 3494 4878 5146 1632
Size: 658 419 785 1384 268 1917
Fragments arranged by size:
1917 1384 785 658 419 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 2463 4330 5307 1203
Size: 1260 1867 977 1327
Fragments arranged by size:
1867 1327 1260 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 3174 4746 3174
Size: 1572 3859
ApaI G_GGCC'C
Cuts at: 2287 2287
Size: 5431
ApaLI G'TGCA_C
Cuts at: 2354 3488 4644 2354
Size: 1134 1156 3141
Fragments arranged by size:
3141 1156 1134
ApoI r'AATT_y
Cuts at: 1493 2498 2740 2966 3118 5227 1493
Size: 1005 242 226 152 2109 1697
Fragments arranged by size:
2109 1697 1005 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2127 2756 1870
Size: 73 116 68 629 4545
Fragments arranged by size:
4545 629 116 73 68
BamHI G'GATC_C
Cuts at: 2761 3128 2761
Size: 367 5064
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2093 2161 2195 2752
Size: 325 217 627 222 84 68 34 557
Cuts at: 2752 4207 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 557 325 222 217 84
68 34
BanII G_rGCy'C
Cuts at: 1391 2066 2287 2411 2750 1391
Size: 675 221 124 339 4072
Fragments arranged by size:
4072 675 339 221 124
BbeI G_GCGC'C
Cuts at: 2013 2097 2199 2013
Size: 84 102 5245
Fragments arranged by size:
5245 102 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3622 3656 1903
Size: 34 1685 34 3678
Fragments arranged by size:
3678 1685 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2515 3323 4533 2515
Size: 808 1210 3413
Fragments arranged by size:
3413 1210 808
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BfrBI ATG'CAT
Cuts at: 2661 2661
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2204 2213 2231
Size: 107 122 71 631 605 532 9 18
Cuts at: 2231 2291 2337 4042 136
Size: 60 46 1705 1525
Fragments arranged by size:
1705 1525 631 605 532 122 107 71
60 46 18 9
BglII A'GATC_T
Cuts at: 2705 5426 2705
Size: 2721 2710
BlpI GC'TnA_GC
Cuts at: 2848 2848
Size: 5431
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2287 2358 2486 3492 4648 1790
Size: 376 121 71 128 1006 1156 2573
Fragments arranged by size:
2573 1156 1006 376 128 121 71
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3162 4120 442
Size: 416 53 83 399 24 1745 958 1753
Fragments arranged by size:
1753 1745 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4091 1239
Size: 2852 2579
Bpu10I CC'TnA_GC
Cuts at: 2157 2715 2157
Size: 558 4873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3556 4421 4719 3556
Size: 865 298 4268
Fragments arranged by size:
4268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4094 882
Size: 617 2595 2219
Fragments arranged by size:
2595 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 2526 5425 2526
Size: 2899 2532
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2094 2196 2276
Size: 53 83 186 936 477 84 102 80
Cuts at: 2276 3238 3620 4255 275
Size: 962 382 635 1451
Fragments arranged by size:
1451 962 936 635 477 382 186 102
84 83 80 53
BsaWI w'CCGG_w
Cuts at: 3864 4262 4536 4683 5094 3864
Size: 398 274 147 411 4201
Fragments arranged by size:
4201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5206
Fragments arranged by size:
5206 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5431
BseYI C'CCAG_C
Cuts at: 2165 2180 2591 4634 5130 2165
Size: 15 411 2043 496 2466
Fragments arranged by size:
2466 2043 496 411 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4858
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2044 2208 2386 2396 2881
Size: 275 186 30 13 164 178 10 485
Cuts at: 2881 3642 4670 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 485 275 186 178 164
30 13 10
BsiHKAI G_wGCw'C
Cuts at: 2358 2721 3492 3577 4648 2358
Size: 363 771 85 1071 3141
Fragments arranged by size:
3141 1071 771 363 85
BsiWI C'GTAC_G
Cuts at: 2334 2334
Size: 5431
BsmI GAATG_Cn'
Cuts at: 2942 3035 2942
Size: 93 5338
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2166 2287 2358 2411 2486 2721
Size: 399 276 100 121 71 53 75 235
Cuts at: 2721 2750 3492 3577 4648 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 235 121 100
85 75 71 53 29
BspEI T'CCGG_A
Cuts at: 5094 5094
Size: 5431
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2786 844
Size: 1942 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2022 2247 3318 1394
Size: 429 199 225 1071 3507
Fragments arranged by size:
3507 1071 429 225 199
BsrDI GCAATG_nn'
Cuts at: 65 1248 3926 4100 65
Size: 1183 2678 174 1396
Fragments arranged by size:
2678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2391 2395 4075 4262 1700
Size: 110 581 4 1680 187 2869
Fragments arranged by size:
2869 1680 581 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 2050 2169 1925
Size: 125 119 5187
Fragments arranged by size:
5187 125 119
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5431
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5431
BstYI r'GATC_y
Cuts at: 1972 2068 2705 2761 3128 3513 3530 4982 5068
Size: 96 637 56 367 385 17 1452 86
Cuts at: 5068 5080 5426 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 637 385 367 346 96 86
56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2367 2367
Size: 5431
BtgI C'CryG_G
Cuts at: 513 1065 2004 2106 2139 2199 2208 2262 513
Size: 552 939 102 33 60 9 54 3682
Fragments arranged by size:
3682 939 552 102 60 54 33 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3741 3761 2947
Size: 794 20 4617
Fragments arranged by size:
4617 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2652 4438 809
Size: 507 1336 1786 1802
Fragments arranged by size:
1802 1786 1336 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2041 2205
Size: 54 1425 50 275 18 198 13 164
Cuts at: 2205 2383 2393 2878 3767 5396 8
Size: 178 10 485 889 1629 43
Fragments arranged by size:
1629 1425 889 485 275 198 178 164
54 50 43 18 13 10
EagI C'GGCC_G
Cuts at: 1537 1812 2028 2041 2205 2383 2393 2878 1537
Size: 275 216 13 164 178 10 485 4090
Fragments arranged by size:
4090 485 275 216 178 164 13 10
EarI CTCTTCn'nnn_
Cuts at: 1353 3361 1353
Size: 2008 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4018 4392 4538 387
Size: 1064 138 2429 374 146 1280
Fragments arranged by size:
2429 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2709 3494 4091 4878 5146 1239
Size: 393 658 419 785 597 787 268 1524
Fragments arranged by size:
1524 787 785 658 597 419 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2283 2283
Size: 5431
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2013 2097 2127 2187 2199 4578 1678
Size: 335 84 30 60 12 2379 2531
Fragments arranged by size:
2531 2379 335 84 60 30 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4072 4104 4146 4178 1373
Size: 32 135 32 2500 32 42 32 2626
Fragments arranged by size:
2626 2500 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3021 669
Size: 988 281 837 246 3079
Fragments arranged by size:
3079 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KasI G'GCGC_C
Cuts at: 2009 2093 2195 2009
Size: 84 102 5245
Fragments arranged by size:
5245 102 84
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4545 4729 1305
Size: 3240 184 2007
Fragments arranged by size:
3240 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 3748 3907 518
Size: 648 2223 359 159 2042
Fragments arranged by size:
2223 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2108 2141 2201 2210 2328 2847 3524
Size: 482 20 33 60 9 118 519 677
Cuts at: 3524 4672 4917 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 519 482 245 118 60
33 20 9
NarI GG'CG_CC
Cuts at: 2010 2094 2196 2010
Size: 84 102 5245
Fragments arranged by size:
5245 102 84
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3940
Fragments arranged by size:
3940 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2205 2878 2205
Size: 673 4758
NsiI A_TGCA'T
Cuts at: 2663 2663
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2467 2789 4334 1132
Size: 97 1238 322 1545 2229
Fragments arranged by size:
2229 1545 1238 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 2463 4330 2463
Size: 1867 3564
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PspOMI G'GGCC_C
Cuts at: 2283 2283
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2783 830
Size: 1953 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
SacII CC_GC'GG
Cuts at: 2109 2142 2202 2211 2109
Size: 33 60 9 5329
Fragments arranged by size:
5329 60 33 9
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SfcI C'TryA_G
Cuts at: 826 1045 2637 2779 3914 4595 4786 826
Size: 219 1592 142 1135 681 191 1471
Fragments arranged by size:
1592 1471 1135 681 219 191 142
SfiI GGCCn_nnn'nGGCC
Cuts at: 2213 2213
Size: 5431
SfoI GGC'GCC
Cuts at: 2011 2095 2197 2011
Size: 84 102 5245
Fragments arranged by size:
5245 102 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2758 1872
Size: 886 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3535 4436 4698 820
Size: 197 926 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2004 2870 513
Size: 552 939 866 3074
Fragments arranged by size:
3074 939 866 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3458 3475 3628 3813 4258 325
Size: 1255 1878 17 153 185 445 1498
Fragments arranged by size:
1878 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2575 3677 5389
Size: 274 80 33 51 1443 598 1102 1712
Cuts at: 5389 96
Size: 138
Fragments arranged by size:
1712 1443 1102 598 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5391 5423 5391
Size: 32 5399
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2756 1870
Size: 886 4545
XmnI GAAnn'nnTTC
Cuts at: 3560 3560
Size: 5431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SrfI SwaI XcmI