Restriction map for
pFN21AE4031
(Circular) (Six-base) MAPSORT of: pFN21AE4031.seq Check: 8273 from: 1 to: 5431
pFN21AE4031 5431 bp
With 182 enzymes: SgfI *
March 28, 2012 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2060 2165 2174 2455 2060
Size: 105 9 281 5036
Fragments arranged by size:
5036 281 105 9
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2070 2248 2752 2070
Size: 178 504 4749
Fragments arranged by size:
4749 504 178
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2531 2579 3494 4878 5146 1632
Size: 899 48 915 1384 268 1917
Fragments arranged by size:
1917 1384 915 899 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 4330 5307 1203
Size: 3127 977 1327
Fragments arranged by size:
3127 1327 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 2100 4160 2100
Size: 2060 3371
AleI CACnn'nnGTG
Cuts at: 1166 2090 1166
Size: 924 4507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2330 2516 1184
Size: 1146 186 4099
Fragments arranged by size:
4099 1146 186
AlwNI CAG_nnn'CTG
Cuts at: 2124 3174 4746 2124
Size: 1050 1572 2809
Fragments arranged by size:
2809 1572 1050
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2740 2966 3118 5227 1493
Size: 1247 226 152 2109 1697
Fragments arranged by size:
2109 1697 1247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 1870
Size: 73 813 4545
Fragments arranged by size:
4545 813 73
BamHI G'GATC_C
Cuts at: 2523 2761 3128 2523
Size: 238 367 4826
Fragments arranged by size:
4826 367 238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2248 2314 2689 2752
Size: 325 217 627 283 178 66 375 63
Cuts at: 2752 4207 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 375 325 283 217 178
66 63
BanII G_rGCy'C
Cuts at: 1391 2750 1391
Size: 1359 4072
BbeI G_GCGC'C
Cuts at: 2693 2693
Size: 5431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2533 2567 3622 3656 1903
Size: 34 596 34 1055 34 3678
Fragments arranged by size:
3678 1055 596 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4042 136
Size: 107 122 71 631 605 2370 1525
Fragments arranged by size:
2370 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2533 2848 2533
Size: 315 5116
Bme1580I G_kGCm'C
Cuts at: 1790 3492 4648 1790
Size: 1702 1156 2573
Fragments arranged by size:
2573 1702 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3162 4120 442
Size: 416 53 83 399 24 1745 958 1753
Fragments arranged by size:
1753 1745 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2501 4091 1239
Size: 923 339 1590 2579
Fragments arranged by size:
2579 1590 923 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3556 4421 4719 2636
Size: 920 865 298 3348
Fragments arranged by size:
3348 920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 4094 882
Size: 617 831 1764 2219
Fragments arranged by size:
2219 1764 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 2177 2367 5425 2177
Size: 190 3058 2183
Fragments arranged by size:
3058 2183 190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2690 3238 3620
Size: 53 83 186 936 549 608 548 382
Cuts at: 3620 4255 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 608 549 548 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3864 4262 4536 4683 5094 3864
Size: 398 274 147 411 4201
Fragments arranged by size:
4201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2241 2271 1178
Size: 30 165 30 838 30 4338
Fragments arranged by size:
4338 838 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2435 2447 2639 1186
Size: 973 276 12 192 3978
Fragments arranged by size:
3978 973 276 192 12
BseYI C'CCAG_C
Cuts at: 4634 5130 4634
Size: 496 4935
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2083 2090 2687 1163
Size: 573 347 7 597 3907
Fragments arranged by size:
3907 597 573 347 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2547 2881 3642 4670 1540
Size: 275 186 546 334 761 1028 2301
Fragments arranged by size:
2301 1028 761 546 334 275 186
BsiHKAI G_wGCw'C
Cuts at: 3492 3577 4648 3492
Size: 85 1071 4275
Fragments arranged by size:
4275 1071 85
BsmI GAATG_Cn'
Cuts at: 2942 3035 2942
Size: 93 5338
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2750 3492 3577 4648 1391
Size: 399 960 742 85 1071 2174
Fragments arranged by size:
2174 1071 960 742 399 85
BspEI T'CCGG_A
Cuts at: 5094 5094
Size: 5431
BspHI T'CATG_A
Cuts at: 2212 3320 5050 2212
Size: 1108 1730 2593
Fragments arranged by size:
2593 1730 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2060 2165 2174 2348 2455 2786 844
Size: 1216 105 9 174 107 331 3489
Fragments arranged by size:
3489 1216 331 174 107 105 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2695 3318 1394
Size: 429 872 623 3507
Fragments arranged by size:
3507 872 623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3926 4100 65
Size: 1183 2678 174 1396
Fragments arranged by size:
2678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4075 4262 1700
Size: 110 2265 187 2869
Fragments arranged by size:
2869 2265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2124 794
Size: 1330 4101
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 2092 1184
Size: 908 4523
BstXI CCAn_nnnn'nTGG
Cuts at: 2375 2375
Size: 5431
BstYI r'GATC_y
Cuts at: 1972 2523 2628 2761 3128 3513 3530 4982 5068
Size: 551 105 133 367 385 17 1452 86
Cuts at: 5068 5080 5426 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 551 385 367 346 133 105
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2088 2415 513
Size: 552 1023 327 3529
Fragments arranged by size:
3529 1023 552 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2554 487
Size: 683 56 1328 3364
Fragments arranged by size:
3364 1328 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3741 3761 2947
Size: 794 20 4617
Fragments arranged by size:
4617 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DraIII CAC_nnn'GTG
Cuts at: 2673 2673
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396
Size: 54 1425 50 275 18 1048 889 1629
Cuts at: 5396 8
Size: 43
Fragments arranged by size:
1629 1425 1048 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3361 1353
Size: 2008 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4018 4392 4538 387
Size: 1064 138 2429 374 146 1280
Fragments arranged by size:
2429 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2501 2531 2579 3494 4091 4878
Size: 393 530 339 30 48 915 597 787
Cuts at: 4878 5146 1239
Size: 268 1524
Fragments arranged by size:
1524 915 787 597 530 393 339 268
48 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2116 2636 2116
Size: 520 4911
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5431
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2693 4578 1678
Size: 1015 1885 2531
Fragments arranged by size:
2531 1885 1015
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 4072 4104 4146
Size: 32 135 32 514 32 1954 32 42
Cuts at: 4146 4178 1373
Size: 32 2626
Fragments arranged by size:
2626 1954 514 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2775 3021 669
Size: 988 281 72 765 246 3079
Fragments arranged by size:
3079 988 765 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KasI G'GCGC_C
Cuts at: 2689 2689
Size: 5431
KpnI G_GTAC'C
Cuts at: 2074 2252 2756 2074
Size: 178 504 4749
Fragments arranged by size:
4749 504 178
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4545 4729 1305
Size: 3240 184 2007
Fragments arranged by size:
3240 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2312 2373 3389 3748 3907 518
Size: 648 924 222 61 1016 359 159 2042
Fragments arranged by size:
2042 1016 924 648 359 222 159 61
MspA1I CmG'CkG
Cuts at: 1606 2067 2417 2529 2847 3524 4672 4917 1606
Size: 461 350 112 318 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 461 350 318 245 112
NarI GG'CG_CC
Cuts at: 2690 2690
Size: 5431
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4879
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2789 4334 1132
Size: 97 1560 1545 2229
Fragments arranged by size:
2229 1560 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2120 2171 2202 2783 830
Size: 1290 51 31 581 3478
Fragments arranged by size:
3478 1290 581 51 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
SacII CC_GC'GG
Cuts at: 2418 2418
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SbfI CC_TGCA'GG
Cuts at: 2171 2783 2171
Size: 612 4819
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SfcI C'TryA_G
Cuts at: 826 1045 2116 2167 2198 2707 2779 3914 4595
Size: 219 1071 51 31 509 72 1135 681
Cuts at: 4595 4786 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1071 681 509 219 191 72
51 31
SfoI GGC'GCC
Cuts at: 2691 2691
Size: 5431
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2758 1872
Size: 886 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 3535 4436 4698 820
Size: 197 926 708 884 901 262 1553
Fragments arranged by size:
1553 926 901 884 708 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 2256 1517
Size: 739 4692
StyI C'CwwG_G
Cuts at: 513 1065 2870 513
Size: 552 1805 3074
Fragments arranged by size:
3074 1805 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2198 3458 3475 3628 3813 4258
Size: 1255 531 87 1260 17 153 185 445
Cuts at: 4258 325
Size: 1498
Fragments arranged by size:
1498 1260 1255 531 445 185 153 87
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 3677 5389
Size: 274 80 33 51 1443 225 1475 1712
Cuts at: 5389 96
Size: 138
Fragments arranged by size:
1712 1475 1443 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 5391 5423 2348
Size: 32 3011 32 2356
Fragments arranged by size:
3011 2356 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2756 1870
Size: 886 4545
XmnI GAAnn'nnTTC
Cuts at: 2564 3560 2564
Size: 996 4435
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI