Restriction map for
pFN21AE4030
(Circular) (Six-base) MAPSORT of: pFN21AE4030.seq Check: 8953 from: 1 to: 5416
pFN21AE4030 5416 bp
With 182 enzymes: SgfI *
March 28, 2012 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2108 2182 2622 2108
Size: 74 440 4902
Fragments arranged by size:
4902 440 74
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2737 2737
Size: 5416
AclI AA'CG_TT
Cuts at: 1563 2715 3543 3916 1563
Size: 1152 828 373 3063
Fragments arranged by size:
3063 1152 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 3479 4863 5131 1632
Size: 746 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1101 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 2157 4315 5292 1203
Size: 954 2158 977 1327
Fragments arranged by size:
2158 1327 977 954
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 4145 4145
Size: 5416
AleI CACnn'nnGTG
Cuts at: 1166 2261 2648 1166
Size: 1095 387 3934
Fragments arranged by size:
3934 1095 387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AlwNI CAG_nnn'CTG
Cuts at: 2497 2614 3159 4731 2497
Size: 117 545 1572 3182
Fragments arranged by size:
3182 1572 545 117
ApaLI G'TGCA_C
Cuts at: 2339 3473 4629 2339
Size: 1134 1156 3126
Fragments arranged by size:
3126 1156 1134
ApoI r'AATT_y
Cuts at: 1493 2725 2951 3103 5212 1493
Size: 1232 226 152 2109 1697
Fragments arranged by size:
2109 1697 1232 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2741 1870
Size: 73 798 4545
Fragments arranged by size:
4545 798 73
BamHI G'GATC_C
Cuts at: 2746 3113 2746
Size: 367 5049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2194 2404 2471 2737 4192
Size: 325 217 627 407 210 67 266 1455
Cuts at: 4192 618
Size: 1842
Fragments arranged by size:
1842 1455 627 407 325 266 217 210
67
BanII G_rGCy'C
Cuts at: 1391 2735 1391
Size: 1344 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 1903
Size: 34 1670 34 3678
Fragments arranged by size:
3678 1670 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4518 3308
Size: 1210 4206
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2059 2063 2684
Size: 107 122 71 631 605 387 4 621
Cuts at: 2684 4027 136
Size: 1343 1525
Fragments arranged by size:
1525 1343 631 621 605 387 122 107
71 4
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2037 2833 2037
Size: 796 4620
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2343 2409 2476 2552 3477 4633 1790
Size: 423 130 66 67 76 925 1156 2573
Fragments arranged by size:
2573 1156 925 423 130 76 67 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4105 442
Size: 416 53 83 399 24 1730 958 1753
Fragments arranged by size:
1753 1730 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2135 2162 4076 1239
Size: 866 30 27 1914 2579
Fragments arranged by size:
2579 1914 866 30 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 882
Size: 617 2580 2219
Fragments arranged by size:
2580 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 3207 493
Size: 711 1059 944 2702
Fragments arranged by size:
2702 1059 944 711
BsaBI GATnn'nnATC
Cuts at: 5410 5410
Size: 5416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3223 3605 4240 275
Size: 53 83 186 936 1690 382 635 1451
Fragments arranged by size:
1690 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 2618 2648 1178
Size: 30 165 30 675 30 510 30 3946
Fragments arranged by size:
3946 675 510 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2684 1186
Size: 1498 3918
BseYI C'CCAG_C
Cuts at: 2426 2582 4619 5115 2426
Size: 156 2037 496 2727
Fragments arranged by size:
2727 2037 496 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4843
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2866 3627 4655 1540
Size: 275 186 57 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2343 3477 3562 4633 2343
Size: 1134 85 1071 3126
Fragments arranged by size:
3126 1134 1071 85
BsmI GAATG_Cn'
Cuts at: 2927 3020 2927
Size: 93 5323
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2343 2409 2476 2552 2735 3477
Size: 399 423 130 66 67 76 183 742
Cuts at: 3477 3562 4633 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 399 183 130 85
76 67 66
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 3305 5035 3305
Size: 1730 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 2182 2622 2771 844
Size: 1264 74 440 149 3489
Fragments arranged by size:
3489 1264 440 149 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 1394
Size: 429 1480 3507
Fragments arranged by size:
3507 1480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 3911 4085 65
Size: 1183 1323 1340 174 1396
Fragments arranged by size:
1396 1340 1323 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 2062 2127 4060 4247 1700
Size: 110 243 9 65 1933 187 2869
Fragments arranged by size:
2869 1933 243 187 110 65 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5416
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2614 794
Size: 1820 3596
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 2317 1184
Size: 1133 4283
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2245 2263 2542 2113
Size: 132 18 279 4987
Fragments arranged by size:
4987 279 132 18
BstYI r'GATC_y
Cuts at: 1972 2746 3113 3498 3515 4967 5053 5065 5411
Size: 774 367 385 17 1452 86 12 346
Cuts at: 5411 1972
Size: 1977
Fragments arranged by size:
1977 1452 774 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 2400 513
Size: 552 939 129 267 3529
Fragments arranged by size:
3529 939 552 267 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2932 3726 3746 2932
Size: 794 20 4602
Fragments arranged by size:
4602 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2136 2152
Size: 54 1425 50 275 18 225 81 16
Cuts at: 2152 2376 2863 3752 5381 8
Size: 224 487 889 1629 43
Fragments arranged by size:
1629 1425 889 487 275 225 224 81
54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2863 1537
Size: 275 243 808 4090
Fragments arranged by size:
4090 808 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 3346 1353
Size: 1993 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2135 2162 2378 3479 4076 4863
Size: 393 473 30 27 216 1101 597 787
Cuts at: 4863 5131 1239
Size: 268 1524
Fragments arranged by size:
1524 1101 787 597 473 393 268 216
30 27
EcoO109I rG'GnC_Cy
Cuts at: 2578 2578
Size: 5416
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 2294 2598 4563 1678
Size: 616 304 1965 2531
Fragments arranged by size:
2531 1965 616 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4057 4089 4131 4163 1373
Size: 32 135 32 2485 32 42 32 2626
Fragments arranged by size:
2626 2485 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2760 3006 669
Size: 988 281 822 246 3079
Fragments arranged by size:
3079 988 822 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KpnI G_GTAC'C
Cuts at: 2741 2741
Size: 5416
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4530 4714 1305
Size: 3225 184 2007
Fragments arranged by size:
3225 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 2378 10
Size: 54 1425 343 306 240 3048
Fragments arranged by size:
3048 1425 343 306 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2261 2648 3374 3733 3892 518
Size: 648 843 252 387 726 359 159 2042
Fragments arranged by size:
2042 843 726 648 387 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2079 2148 2206 2832 3509 4657 4902
Size: 438 35 69 58 626 677 1148 245
Cuts at: 4902 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 626 438 245 69 58
35
NaeI GCC'GGC
Cuts at: 2055 2064 2055
Size: 9 5407
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3925
Fragments arranged by size:
3925 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NgoMIV G'CCGG_C
Cuts at: 2053 2062 2053
Size: 9 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2774 4319 1132
Size: 97 1545 1545 2229
Fragments arranged by size:
2229 1545 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2571 1348
Size: 1223 4193
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PfoI T'CCnGG_A
Cuts at: 2364 2364
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PmlI CAC'GTG
Cuts at: 2263 2263
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2578
Size: 5416
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2193 2610 2768 830
Size: 1363 417 158 3478
Fragments arranged by size:
3478 1363 417 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
PvuII CAG'CTG
Cuts at: 2148 2148
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SanDI GG'GwC_CC
Cuts at: 2578 2578
Size: 5416
SbfI CC_TGCA'GG
Cuts at: 2193 2768 2193
Size: 575 4841
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2189 2606 2656 2764 3899 4580 4771
Size: 219 1144 417 50 108 1135 681 191
Cuts at: 4771 826
Size: 1471
Fragments arranged by size:
1471 1144 1135 681 417 219 191 108
50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4421 4683 820
Size: 197 926 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2774 1132
Size: 1642 3774
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2855 513
Size: 552 939 851 3074
Fragments arranged by size:
3074 939 851 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 3443 3460 3613 3798 4243 325
Size: 1255 693 1170 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1170 693 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3662 5374
Size: 274 80 33 51 1443 117 1568 1712
Cuts at: 5374 96
Size: 138
Fragments arranged by size:
1712 1568 1443 274 138 117 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2333 2365 2406 2438 2618 2650 5376 5408 2333
Size: 32 41 32 180 32 2726 32 2341
Fragments arranged by size:
2726 2341 180 41 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 3545 3545
Size: 5416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI